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The aim of this study was to assess the effect of stress on a previously acquired motor coordination. Following a longitudinal learning experiment, four participants performed oscillations on a ski simulator, either in normal or stressful conditions. The results showed that the amplitude of the oscillations decreased under stress, but no significant effect was seen regarding coordination, suggesting the strong resistance to stress of overlearned behaviour. Nevertheless, for one participant, a transient regression towards a former stage of learning was observed. This result was consistent with the regression hypothesis formulated by Fuchs (1962). 相似文献
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Linda Desbois Adrien Padirac Shohei Kaneda Anthony J. Genot Yannick Rondelez Didier Hober Dominique Collard Teruo Fujii 《Biomicrofluidics》2012,6(4)
Water-in-oil microdroplets offer microreactors for compartmentalized biochemical reactions with high throughput. Recently, the combination with a sol-gel switch ability, using agarose-in-oil microdroplets, has increased the range of possible applications, allowing for example the capture of amplicons in the gel phase for the preservation of monoclonality during a PCR reaction. Here, we report a new method for generating such agarose-in-oil microdroplets on a microfluidic device, with minimized inlet dead volume, on-chip cooling, and in situ monitoring of biochemical reactions within the gelified microbeads. We used a flow-focusing microchannel network and successfully generated agarose microdroplets at room temperature using the “push-pull” method. This method consists in pushing the oil continuous phase only, while suction is applied to the device outlet. The agarose phase present at the inlet is thus aspirated in the device, and segmented in microdroplets. The cooling system consists of two copper wires embedded in the microfluidic device. The transition from agarose microdroplets to microbeads provides additional stability and facilitated manipulation. We demonstrate the potential of this method by performing on-chip a temperature-triggered DNA isothermal amplification in agarose microbeads. Our device thus provides a new way to generate microbeads with high throughput and no dead volume for biochemical applications. 相似文献
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Christelle Didier 《European Journal of Engineering Education》2000,25(4):325-335
This paper relates to the development of non-technical education (and to ethics) in engineering curricula in Europe and particularly in France. Two projects being followed at the Centre de recherche en éthique de l'ingénieur (CREI) at the Catholic University of Lille (France) are discussed: the first is an engineering ethics course which has been running for 6 years in a state engineering school, where there is a strong emphasis on analysing the ethical issues of the students' first work experience. The second has to do with the writing of a European handbook on engineering ethics which gives us good insight into how engineering ethics as a discipline is shaped by the cultural background of those who develop it (dominant philosophical tradition, religious background, the way the engineering profession is organized and education). Two main approaches seem to be prevalent in Europe today: the first relates to professional ethics as discussed in the USA since the 1950s, the second relates to a new discipline that is developing in Europe, that of the ethics of technology. 相似文献
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Recent experiments on the ski simulator produced ambiguous results and raised unanswered questions concerning the true nature of "novice" behavior and the occurrence of behavioral changes during learning. The aim of the present experiment was to analyze the evolving behavior of three beginners during six practice sessions on a ski simulator The position of the apparatus platform was recorded as time series and used for constructing dynamical models, including stiffness and damping functions. The results showed that novices tended to exploit a Rayleigh damping behavior during the first trials and then transition toward a van der Pol damping. These results replicate previous observations by Nourrit, Delignieres, Caillou, Deschamps, and Lauriot (2003) and suggest the transition to the expert behavior could arise early in practice, when the task is of moderate difficulty. The discussion focuses on the properties of the observed learning dynamics and proposes a global conceptualization for acquiring complex motor skills. 相似文献
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Sheng-yong Wu Hui-hui Jia Didier Hans Jing Lan Li-ying Wang Jing-xue Li Yue-zeng Cai 《Journal of Zhejiang University. Science. B》2009,10(7):499-504
Objective: To demonstrate the validity and reliability of volumetric quantitative computed tomography (vQCT) with multi-slice computed tomography (MSCT) and dual energy X-ray absorptiometry (DXA) for hip bone mineral density (BMD) measurements, and to compare the differences between the two techniques in discriminating postmenopausal women with osteoporosis-related vertebral fractures from those without. Methods: Ninety subjects were enrolled and divided into three groups based on the BMD values of the lumbar spine and/or the femoral neck by DXA. Groups 1 and 2 consisted of postmenopausal women with BMD changes ＜-2SD, with and without radiographically confirmed vertebral fracture (n=11 and 33, respectively).Group 3 comprised normal controls with BMD changes ≥-1SD (n=46). Post-MSCT (GE, LightSpeed16) scan reconstructed images of the abdominal-pelvic region, 1.25 mm thick per slice, were processed by OsteoCAD software to calculate the following parameters: volumetric BMD values of trabecular bone (TRAB), cortical bone (CORT), and integral bone (INTGL) of the left femoral neck, femoral neck axis length (NAL), and minimum cross-section area (mCSA). DXA BMD measurements of the lumbar spine (AP-SPINE) and the left femoral neck (NECK) also were performed for each subject. Results: The values of all seven parameters were significantly lower in subjects of Groups 1 and 2 than in normal postmenopausal women (P＜0.05, respectively).Comparing Groups 1 and 2, 3D-TRAB and 3D-INTGL were significantly lower in postmenopausal women with vertebral fracture(s) [(109.8±9.61) and (243.3±33.0) mg/cm3, respectively] than in those without [(148.9±7.47) and (285.4±17.8) mg/cm3,respectively] (P＜0.05, respectively), but no significant differences were evident in AP-SPINE or NECK BMD. Conclusion: the femoral neck-derived volumetric BMD parameters using vQCT appeared better than the DXA-derived ones in discriminating osteoporotic postmenopausal women with vertebral fractures from those without, vQCT might be useful to evaluate the effect of osteoporotic vertebral fracture status on changes in bone mass in the femoral neck. 相似文献
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ABSTRACT In light of ongoing debates about religious education as hermeneutical, this contribution proposes a ‘hermeneutical-communicative’ (HCM) paradigm for RE through the development of a twofold reflection: (a) a critical (re-)evaluation of the theological and anthropological foundations for RE in light of (b) a context marked by religious and philosophical diversity, disaffiliation and ‘areligiosity’. In this way, the HCM approach proposes an identity for RE that lies at the intersection of ‘hermeneutical’ and ‘dialogical’. Drawing upon theologies of interreligious dialogue, this contribution first analyses four paradigms for RE (exclusivism, inclusivism, pluralism, particularism) and then advocates for a hermeneutical-communicative approach characterised by an emphasis on interreligious ‘literacy’, philosophical and religious hospitality and inter-hermeneutical dialogue. Such a paradigm results in a number of implications for practice, including sensitivity to ‘big questions’ in life, engagement with the Gospels and the faith tradition, respect and appreciation for other avenues in the search for meaning and identity, and attention to the personal growth of young people. 相似文献
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