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验证收缩骨骼肌细胞葡萄糖转运蛋白4（GLUT4）基因的表达在转录水平上是否受转录抑制子Ⅱ型HDAC5蛋白（简写为HDAC5）的调节。研究运用运动模拟信号5-氨基-4-甲酰胺咪唑核糖核苷酸（AICAR）和HDACs抑制剂SCRIPTAID孵育骨骼肌细胞。用蛋白印迹技术测定HDAC5蛋白表达,定量RT-PCR法检测GLUT4 mRNA表达。与无AICAR的对照组相比,AICAR组骨骼肌细胞内HDAC5减少了29%,并伴随有124% GLUT4 mRNA的增加,但肌细胞内HDAC5的蛋白总量无变化;使用HDACs抑制剂SCRIPTAID刺激骨骼肌细胞能够明显增加核心组蛋白乙酰化水平和上调GLUT4基因的表达。这些结果提示,通过AICAR和SCRIPTAID分别减少或抑制核HDAC5均能上调骨骼肌细胞GLUT4基因的转录,HDAC5可能在转录水平上对肌肉收缩引起的GLUT4基因的表达起着重要的调节作用。 相似文献
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OBJECTIVE: The study assessed the coping strategies of MEDLIB-L subscribers during an unexpected disruption in the list's service. METHODS: An online survey of MEDLIB-L subscribers was performed following a six-day service outage in August 1999. RESULTS: Respondents' information needs resulted in two distinct coping strategies. Subscribers without a recognized information need or an information need determined to be not pressing coped by waiting out the interruption. Subscribers with pressing information needs turned to alternative methods of resolving these needs. CONCLUSIONS: While most respondents missed the list and the assistance that it provided, many did not feel that the outage required significant coping strategies. The outage was viewed as a "minor stressor" and did not require secondary-level assessment of the availability and suitability of alternative resources. 相似文献
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MacCall SL 《Medical reference services quarterly》1997,16(4):69-74
The metastrategy for World-Wide Web information retrieval was presented as an integrated approach to end-user searching in clinical medicine, although its application may be made to any search for WWW information. Certain distinctions, such as between anticipated and unanticipated clinical information needs and between WWW catalogs for retrieving Web sites and WWW databases for retrieving Web pages, were made. These distinctions are important in the end-user strategy because they help maximize the productivity of the WWW for clinicians. 相似文献
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