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以结缕草属的马尼拉草、结缕草和中华结缕草进行了试管繁殖的研究.以马尼拉草匍匐茎为外植体,通过芽直接增殖和愈伤组织分化途径形成了2个试管无性系;从结缕草和中华结缕草的实生苗中也筛选出了若干个试管无性系.嫩极丛在1/2MS培养基添加IBA或 NAA0.5 mg/L、IBA0.25mg/L+NAAO.25mg/L上生根,经38 d培养,马尼拉草生根率为100%,结缕草为88%~90%.移栽成活率马尼拉草在95%以上,结缕草高于90%.试管草移栽后生长旺盛。 相似文献
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若干暖季型草坪草育种和组织培养研究进展 总被引:15,自引:0,他引:15
系统地阐述了若干主要暖季型草坪草(狗牙根、野牛草和结缕草)在育种和组织培养上的研究进展,所提供的资料对于我国草坪草的研究和应用既有理论参考价值,更具有现实的指导意义。 相似文献
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试管筛选耐盐的结缕草再生植株 总被引:2,自引:0,他引:2
测定了结缕草(Zoysia japonica)栽培品种Zenith商品种子实生苗的耐盐能力、种子愈伤组织发生的耐盐性、胚性愈伤组织系的耐盐能力及再生能力。在试管中筛选出了比该栽培品种群体耐盐性增强的体细胞无性系变异植株。初步的栽培试验表明,这些变异植株的耐盐性强于对照植株。 相似文献
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柴明良 汪炳良 KIM Jae-yeoul LEE Jong-min KIM Doo-hwan 《Journal of Zhejiang University. Science. B》2003,(3)
INTRODUCTIONCreepingbentgrass (AgrostispalustrisHuds.)isanoutstandingcool seasonspeciesavailableforuseongolfcourseputtinggreens,tees,andcloselymowedfairways.Althoughco lonialbentgrass (AgrostistenuisSibth .Fl.Ox en .)isnotwelladaptedtotheverylowmowingheig… 相似文献
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Embryogenic calli were induced from the seeds of creeping bentgrass (Agrostis palustris Huds.) cv. Regent and colonial bentgrass (Agrostis Tenuis Sibth. Fl. Oxen.) cv. Tiger. The embryogenic calli were precultured on fresh medium for 4-7 days and then co-cultivated with Agrobacterium tumefaciens, LBA4404, which contains plasmid vector-pSBGM harboring bar coding region, synthetic green fluorescent protein (sGFP) coding region and matrix attachment region (MAR). After 3 days of co-cultivation, the calli were washed thoroughly and transferred to MS medium containing 2 mg/L of 2, 4-D, 12-15 mg/L phosphinothricin (PPT) and 250 mg/L of cefotaxime. After 2-3 months of selection, the actively growing calli of 'Regent' and 'Tiger' were transferred to MS medium with 12-15 mg/L PPT and 250 mg/L cefotaxime for regeneration. The putative transformants were maintained on MS medium with 3 mg/L PPT for long period but control died within 1 month. After establishing in greenhouse, the transformants also showed strong resistance to 0.4% of herbicide Basta but control plants died within 2 weeks. Under confocal microscope, both young leaves and roots showed significant GFP expression. PCR analysis revealed the presence of a DNA fragment of GFP gene at the expected size (380 bp) in the transformants and its absence in a randomly selected control plant. 相似文献
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草坪草转基因研究进展 总被引:28,自引:0,他引:28
系统地回顾了草坪草转基因研究的进展。分析了目前转基因技术在草坪草育种中的局限性。对今后草坪草转基因的发展趋势提出了看法。本文所提供的资料对草坪草的研究和应用具有重要的参考价值。 相似文献
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高职园艺技术专业是农业院校的主干专业之一,国内现有高职园艺技术专业人才培养方案及课程设置普遍存在着课程门类太多,专业特色不明显;综合实训环节相对薄弱以及创新创业机制平台缺乏等问题,应采取根据实际情况确立园艺专业的培养目标,建立学科特色;按模块设立课程,推进课程综合化,重点抓好核心课程和综合素质课程建设;重视实践教学,培养学生的就业与创业能力等措施进行改革,才能使高职园艺技术更好地实现人才培养目标,为社会培养更多技能型人才。 相似文献
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