cDNA cloning and sequence analysis of genome segments S8 from rice black-streaked dwarf virus     

张恒木  陈剑平  薛庆中  雷娟利 《浙江大学学报(A卷英文版)》2002,3(2):216-222

Genome segments S8 of two Chinese isolates of rice black-streaked dwarf virus (RBSDV), one from Zhejiang Province and another from Hebei Province, were amplified by RT-PCR and sequenced. Both segments consisted of 1936 nts in full length (EMBL accession numbers were AJ297431 and AJ297432, respectively) and contained only one big open reading frame which encoded a polypeptide with molecular weight of 68kD. The two Chinese isolates shared 94.0% and 96.5% identity at nucleotide and amino acid level, respectively. They shared 94.5-94.9% and 92.5-92.9% homology with S8 of RBSDV Japanese isolate at nucleotide and amino acid level, respectively; shared 85.1-87.6% and 91.7-91.9% homology with S7 of Italian MRDV (maize rough dwarf virus).  相似文献   

通过组织培养筛选水稻耐盐突变体的初步研究     

严菊强  薛庆中  沈圣泉 《科技通报》1989,(3)

应用组织培养技术筛选耐盐突变体已在许多作物上进行。本试验观察了愈伤组织对不同氯化钠浓度培养基的反应。试图为水稻耐盐性育种制订筛选程序并提供耐盐种质。 1.材料和方法选用本课题组育成的3个晚粳稻品种(Oryza sativa L.)T79110,浙农大45(即T8245),浙农大40(即T8340)供试。每个品种各取10克种子。去壳后置0.2％升汞液  相似文献   

人类基因组计划及其伦理学问题   总被引：1，自引：0，他引：1  

刘庆坡  薛庆中 《中国科学基金》2002,16(3):165-168

本文概述了国际HGP和中国HGP的研究现状，并讨论了由此带来的ELSI 问题。  相似文献