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Objective: To determine the effective nucleotide sites of ribozymes against HCV, and obtain a highly effective, nontoxic and
inexpensive antisense ribozyme specific for HCV. Methods: Two effective ribozymes, targeted to HCV 5’NC region and C region,
were designed and synthesized. Eukaryotic expression vectors, pSV2-gpt. CD-SR (, containing either HCRZNC or HCRZC were constructued
and transfected into MT-2 cells, which had been infected by HCV. Quantitative PCR and hydridization were used to determine
the effect of inhibition of HCV by ribozymes. Results: HCRZNC and HCRZC suppressed the replication of HCV by 54.7% and 62.1%,
respectively. Furthermore, when both ribozymes were cotransfected into cells, they suppressed replication by 78.8%. Conclusion:
Two specific antisense ribozymes have strong inhibitory effects on the replication of HCV in cultured cells, and have better
effect when used together. 相似文献
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Journal of Zhejiang University-SCIENCE B - This study aimed to investigate DNA sequences that are substantially homologous to the corresponding RNA sequence sections of the hepatitis C virus (HCV).... 相似文献
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The new prevalence data regarding the estimated global number of human immunodeficiency virus positive (HIV+) cases, i.e., including people who are either aware or unaware of their HIV infection in 2010, lead many to wonder why the
increase in incidence has reached today’s unprecedented level and escalated within such a short time. This, in spite of prevention
campaigns in countries affected by HIV/acquired immune deficiency syndrome (AIDS) with their urgent messages aimed at preventing
HIV transmission by promoting changes in individual’s behavior. This article analyzes the background of the prevention strategies,
in particular their political, social and legal concepts in terms of human rights, and reveals traits of human behavior not
considered thus far. A radical reappraisal is necessary, at social and legislative levels, as well as options additional to
current concepts. When ethical issues come up, they become blamed for outmoded moralistic positions. However, ignoring the
reality has led to dire consequences from prioritizing individual human rights over society’s collective need to prevent the
spread of HIV. 相似文献
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ＩＮＴＲＯＤＵＣＴＩＯＮＲｉｂｏｚｙｍｅｗａｓｆｉｒｓｔｄｅｓｃｒｉｂｅｄｂｙＣｅｃｈａｎｄＡｌｔｍａｎｉｎ 1 981 .ＴｈｉｓｆｏｒｍｏｆＲＮＡｈａｄｈｉｇｈｌｙｓｐｅｃｉｆｉｃｅｎｄｏｎｕｃｌｅａｓｅａｃｔｉｖｉｔｉｅｓｔｏＲＮＡ (Ｋｒｕｇｅｒｅｔａｌ.,1 982 ) .Ｒｉｂｏｚｙｍｅｓｈａｖｅｔｈｒｅｅｓｔｒｕｃｔｕｒｅｓ:ｈａｍｍｅｒｈｅａｄ ,ｈａｉｒｐｉｎａｎｄａｘｅｒｉｂｏｚｙｍｅｓ.Ａｍｏｎｇｔｈｅｍ ,ｔｈｅｈａｍｍｅｒｈｅａｄｒｉｂｏｚｙｍｅｈａｓａｓｉｍｐｌｅｒｓｅｃｏｎｄａｒｙｓｔｒｕｃ… 相似文献
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