Roles of paper-based reading ability and ICT-related skills in online reading performance     

Liu  I-Fang  Ko  Hwa-Wei 《Reading and writing》2019,32(4):1037-1059

Reading and Writing - Online reading requires a hybrid of reading and information and communication technology (ICT)-related skills. The purpose of this study was to explore the role of paper-based...  相似文献   

A dielectrophoretic chip with a roughened metal surface for on-chip surface-enhanced Raman scattering analysis of bacteria     

I-Fang Cheng  Chi-Chang Lin  Dong-Yi Lin    Hsien-Chang Chang 《Biomicrofluidics》2010,4(3)

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Antibiotic susceptibility test based on the dielectrophoretic behavior of elongated Escherichia coli with cephalexin treatment     

Chung CC  Cheng IF  Yang WH  Chang HC 《Biomicrofluidics》2011,5(2):21102

This study reports the use of dielectrophoresis (DEP), which determined the crossover frequency (cof) of antibiotic-induced elongation of Escherichia coli (E. coli) with regard to the rapid antibiotic susceptibility test (AST). Different dielectric properties and elongation rates of E. coli are caused by various concentrations of cephalexin treatment. According to the authors' results, significant changes in the cof of bacteria treated with 32 μg∕ml antibiotic for 60 min can be found by using a quadruple electrode array, and the results of DEP-based AST correspond with that of agar dilution method. Utilizing this approach could greatly reduce the period of bacteria growth, and obtain the minimum inhibition concentration of E. coli to cephalexin.  相似文献   

An integrated dielectrophoretic chip for continuous bioparticle filtering, focusing, sorting, trapping, and detecting   总被引：1，自引：0，他引：1  

I-Fang Cheng  Hsien-Chang Chang  Diana Hou    Hsueh-Chia Chang 《Biomicrofluidics》2007,1(2)

Multi-target pathogen detection using heterogeneous medical samples require continuous filtering, sorting, and trapping of debris, bioparticles, and immunocolloids within a diagnostic chip. We present an integrated AC dielectrophoretic (DEP) microfluidic platform based on planar electrodes that form three-dimensional (3D) DEP gates. This platform can continuously perform these tasks with a throughput of 3 μL∕min. Mixtures of latex particles, Escherichia coli Nissle, Lactobacillus, and Candida albicans are sorted and concentrated by these 3D DEP gates. Surface enhanced Raman scattering is used as an on-chip detection method on the concentrated bacteria. A processing rate of 500 bacteria was estimated when 100 μl of a heterogeneous colony of 10⁷ colony forming units ∕ml was processed in a single pass within 30 min.  相似文献   

Ripple structure-generated hybrid electrokinetics for on-chip mixing and separating of functionalized beads     

I-Fang Cheng  Sheng-Chuan Chiang  Cheng-Che Chung  Trai-Ming Yeh  Hsien-Chang Chang 《Biomicrofluidics》2014,8(6)

We present an electrokinetics-based microfluidic platform that is capable of on-chip manipulating, mixing, and separating microparticles through adjusting the interrelated magnitudes of dielectrophoresis and AC electroosmosis. Hybrid electrokinetic phenomenon is generated from an electric field-induced micro-ripple structure made of ultraviolet-curable glue. Size-dependent particle separation and selective removal over the ripple structure is demonstrated successfully. Varying the waveform from sine-wave to square-wave allows generating a fluid convection at specific positions to mix the antibody-functionalized beads and antigen. Potential application in the bead-based immunoassay was also demonstrated for immuno-reaction and subsequently separating the bead-bead aggregate and non-binding beads on-chip.  相似文献