排序方式： 共有7条查询结果，搜索用时 171 毫秒
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Sitthichai IAMSAARD Thawatchai PRABSATTROO Wannisa SUKHORUM Supaporn MUCHIMAPURA Panee SRISAARD Nongnut UABUNDIT Wipawee THUKHAMMEE Jintanaporn WATTANATHORN 《Journal of Zhejiang University. Science. B》2013,14(3):247-252
Objective:To investigate the effect of Anethum graveolens(AG) extracts on the mounting frequency,histology of testis and epididymis,and sperm physiology.Methods:Male rats induced by cold immobilization before treating with vehicle or AG extracts [50,150,and 450 mg/kg body weight(BW)] via gastric tube for consecutive 1,7,and 14 d were examined for mounting frequency,testicular phosphorylation level by immunoblotting,sperm concentration,sperm acrosome reaction,and histological structures of testis and epididymis,respectively.Results:AG(50 mg/kg BW) significantly increased the mounting frequency on Days 1 and 7 compared to the control group.Additionally,rat testis treated with 50 mg/kg BW AG showed high levels of phosphorylated proteins as compared with the control group.In histological analyses,AG extract did not affect the sperm concentration,acrosome reaction,and histological structures of testis and epididymis.Conclusions:AG extract enhances the aphrodisiac activity and is not harmful to sperm and male reproductive organs. 相似文献
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Sitthichai IAMSAARD Supatcharee ARUN Jaturon BURAWAT Wannisa SUKHORUM Jintanaporn WATTANATHORN Somsak NUALKAEW Bungorn SRIPANIDKULCHAI 《Journal of Zhejiang University. Science. B》2014,15(4):405-408
通过对余甘子不同部位（叶、枝和树皮）水提物的总酚含量的测定及体外抗氧化活性的研究,为其体内及临床试验研究奠定了基础。余甘子叶、枝和树皮的水提物皆具有抗氧化活性,其中树皮中的总酚含量最高,抗氧化活性最强。 相似文献
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Sitthichai IAMSAARD Jaturon BURAWAT Pipatpong KANLA Supatcharee ARUN Wannisa SUKHORUM Bungorn SRIPANIDKULCHAI Nongnut UABUNDIT Jintanaporn WATTATHORN Wiphawi HIPKAEO Duriya FONGMOON Hisatake KONDO 《Journal of Zhejiang University. Science. B》2014,15(6):548-555
Background
Ketoconazole (KET), an antifungal drug, has adverse effects on the male reproductive system. Pre-treatments with antioxidant plant against testicular damage induced by KET are required. The flowers of Clitoria ternatea (CT) are proven to have hepatoprotective potential. However, the protective effect on KET-induced testicular damage has not been reported.Objective
To investigate the protective effect of CT flower extracts with antioxidant activity on male reproductive parameters including sperm concentration, serum testosterone level, histopathology of the testis, and testicular tyrosine phosphorylation levels in rats induced with KET.Methods
The antioxidant activity of CT flower extracts was determined using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) assays. Male rats were treated with CT flower extracts (10, 50, or 100 mg/kg BW) or distilled water via a gastric tube for 28 d (preventive period: Days 1–21) and induced by KET (100 mg/kg BW) via intraperitoneal injection for 7 d (induction period: Days 22–28). After the experiment, all animals were examined for the weights of the testis, epididymis plus vas deferens and seminal vesicle, serum testosterone levels, sperm concentration, histological structures and diameter of testis, and testicular tyrosine phosphorylation levels by immunoblotting.Results
The CT flower extracts had capabilities for DPPH scavenging and high reducing power. At 100 mg/kg BW, the extract had no toxic effects on the male reproductive system. Significantly, in CT+KET groups, CT flower extracts (50 and 100 mg/kg BW) alleviated the reduction of reproductive organ weight parameters, testosterone levels, and sperm concentration. In addition, CT flower extracts gave protection from testicular damage in KET-induced rats. Moreover, in the CT100+KET group, CT flower extracts significantly enhanced the expression of a testicular 50-kDa tyrosine phosphorylated protein compared with that of other groups.Conclusions
C. ternatea flower extracts possessing antioxidant activity are not harmful to the male reproductive system and can protect against testicular damage in KET-induced rats. 相似文献4.
Sitthichai IAMSAARD Jaturon BURAWAT Pipatpong KANLA Supatcharee ARUN Wannisa SUKHORUM Bungorn SRIPANIDKULCHAI Nongnut UABUNDIT Jintanaporn WATTATHORN Wiphawi HIPKAEO Duriya FONGMOON Hisatake KONDO 《Journal of Zhejiang University. Science. B》2014,(6)
研究目的:研究蝶豆花提取物的抗氧化活性及其对酮康唑(KET)诱导雄性大鼠睾丸损伤的保护作用。创新要点:对蝶豆花提取物保护酮康唑引起的雄性动物睾丸损伤进行研究,为减轻抗真菌药物酮康唑的生殖毒性提供理论依据和解决途径。研究方法:采用2,2-二苯基-1-苦肼基自由基(DPPH)分析法和亚铁还原能力实验法(FRAP)来测定蝶豆花提取物的抗氧化活性。在为期28天的动物试验中,前21天为预防期,第21至28天为KET诱导期。整个试验期中,对雄性大鼠进行蝶豆花提取物(0、50、100 mg/kg BW)灌胃饲养;在诱导期,同时腹腔注射KET(100 mg/kg BW)。试验结束后,测定睾丸、附睾和输精管以及精囊的重量、血清睾酮水平、精子浓度、组织结构、睾丸的直径和睾丸酪氨酸磷酸化水平。重要结论:蝶豆花提取物具有清除DPPH自由基能力和较高的还原能力,其在100 mg/kg BW下对雄性大鼠生殖系统无毒。50、100 mg/kg BW蝶豆花提取物能改善KET引起的生殖器官重量下降、血清睾酮水平和精子浓度,降低KET引起的睾丸损伤。与其他组相比,100 mg/kg BW蝶豆花提取物能显著提高睾丸中50-kDa的酪氨酸磷酸化蛋白的表达。由此可见,具有抗氧化活性的蝶豆花提取物不会损伤雄性生殖系统,而且具有保护KET诱导大鼠睾丸损伤的作用。 相似文献
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Supatcharee Arun Jaturon Burawat Supataechasit Yannasithinon Wannisa Sukhorum Akgpol Limpongsa Sitthichai Iamsaard 《Journal of Zhejiang University. Science. B》2018,19(12):948-959
Stress affects the male reproductive system and can cause sub-fertility or infertility. Although Phyllanthus emblica L. (PE) extract has been shown to have high antioxidant capacity and protective properties in damaged tissue, the preventive effects of PE extract on testicular function from stress-related impairment have never been demonstrated. This study aimed to investigate the effects of PE aqueous leaf extract on testicular impairment and protein marker changes in rats suffering from chronic stress. Adult male rats were divided into four groups: a control group, a chronic stress (CS) group, and two groups with CS that received different doses of PE extract (50 or 100 mg/kg body weight (BW)). In the treatment groups, the animals were given PE extract daily before stress induction for 42 consecutive days. Stress was induced through immobilization (4 h/d) followed by forced cold swimming (15 min/d). Sperm quality and the histology of the testes and caudal epididymis were examined, as were levels of serum corticosterone, testosterone, and malondialdehyde (MDA). The expressions of testicular steroidogenic acute regulatory (StAR) and tyrosine-phosphorylated proteins were investigated using immuno-Western blot analysis, as these proteins are assumed to play important roles in spermatogenesis and androgen synthesis. The results showed that PE (50 mg/kg BW) significantly increased sperm concentration and testosterone levels, while decreasing corticosterone levels, MDA levels, sperm head abnormalities, and acrosome-reacted sperm in CS rats. In addition, PE at both doses was found to diminish testicular histopathology in the CS rats. We also found that 50 mg/kg BW of PE significantly improved StAR protein expression and altered the intensities of some tyrosine-phosphorylated proteins in testis. We conclude that PE leaf extract at 50 mg/kg BW can prevent testicular damage in rats with CS. 相似文献
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Sitthichai IAMSAARD Supatcharee ARUN Jaturon BURAWAT Wannisa SUKHORUM Jintanaporn WATTANATHORN Somsak NUALKAEW Bungorn SRIPANIDKULCHAI 《Journal of Zhejiang University. Science. B》2014,(4)
通过对余甘子不同部位(叶、枝和树皮)水提物的总酚含量的测定及体外抗氧化活性的研究,为其体内及临床试验研究奠定了基础。余甘子叶、枝和树皮的水提物皆具有抗氧化活性,其中树皮中的总酚含量最高,抗氧化活性最强。 相似文献
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