Effect of <Emphasis Type="SmallCaps">l</Emphasis>-Thyroxine on Micronuclei Frequency in Peripheral Blood Lymphocytes in Clinical and Experimental Conditions     

Yogita S. Raut  Uma S. Bhartiya  Purushottam Kand  Rohini W. Hawaldar  Ramesh V. Asopa  Lebana J. Joseph  MGR Rajan 《Indian journal of clinical biochemistry : IJCB》2016,31(1):99-103

The aim was to study the genotoxic effect of high concentration of thyroxine (T₄) in vivo in peripheral blood lymphocytes (PBL) of the patients suffering from thyroid disorders. The effect was compared by performing in vitro experiments with addition of increasing concentration of T₄ (0.125–1 µM) in whole blood samples from healthy donors. Cytokinesis-blocked micronuclei (CBMN) assay method was used to assess the DNA damage in the PBL. The study included 104 patients which were grouped as control (n = 49), hyperthyroid (n = 31) and hypothyroid (n = 24). A significant increase in micronuclei (MN) frequency was observed in hyperthyroid patients when compared with the hypothyroid and euthyroid group thereby suggesting increased genotoxicity in hyperthyroidism (p < 0.001). A significant increase in MN frequency was observed at T₄ concentration of 0.5 µM and above when compared to lower T₄ concentrations (0.125 and 0.25 µM) and basal in in vitro experiments (p = 0.000). The results indicate that the T₄ in normal concentration does not exhibit the genotoxic effect, as observed in both the in vivo and in vitro experiments. The toxicity of T₄ increases at and above 0.5 μM concentration in vitro. Therefore acute T₄ overdose should be handled promptly and effectively so as to avoid the possible genotoxic effect of high concentration of T₄ in vivo.  相似文献   

Antioxidant and lipid lowering activities of Indian black tea     

Ramesh Chander  A. K. Khanna  Kanwal Raj  A. K. Rastogi 《Indian journal of clinical biochemistry : IJCB》2005,20(1):153-159

Indian black tea; CTC leaf and dust, produced by Tata Tea Limited, Kolkata, (India) was studiedin vitro as potential scavenger of oxygen free radicals. Super oxide anions were generated in a system containing xanthine—xanthine oxidase (enzymic system) and by NADH- phenozine methosulphate (non enzymic system). Anions were assayed in terms of uric acid formation and reduction of nitroblue tetrazolium salt, which were shown to be suppressed by tea extracts. Extracts from both leaf and dust also inhibted the formation of hydroxyl radicalsin vitro in the enzymic system comprising hypoxanthine—Cu⁺²—sodium ascorbate and xanthine oxidase and in non enzymic system of deoxyribose—Cu⁺²—sodium ascorbate and H₂O₂ as well as the Cu⁺² induced lipid peroxidation in human low density lipoprotein. Feeding with black tea in normal rats for sixty days increased their antioxidant activity and their liver microsomes were shown to be protected against peroxidation of lipids as stimulated by metal ions with enzymic or non enzymic reactants. Furthermore feeding with tea extracts in normal as well as triton WR—1339 induced hyperlipidemic rats caused decrease in their plasma levels of total cholesterol, phospholipids and triglycerides. The antioxidant and lipid lowering activities of both extracts from CTC leaf and dust tea was comparable and may be due to the presence of natural products like catechin and others.  相似文献   

Status and Interrelationship of Zinc,Copper, Iron,Calcium and Selenium in Prostate Cancer     

Bhupendra Pal Singh  Shailendra Dwivedi  Urmila Dhakad  Ramesh Chandra Murthy  Vimal Kumar Choubey  Apul Goel  Satya Narayan Sankhwar 《Indian journal of clinical biochemistry : IJCB》2016,31(1):50-56

Deficiency or excess of certain trace elements has been considered as risk factor for prostate cancer. This study was aimed to detect differential changes and mutual correlations of selected trace elements in prostate cancer tissue versus benign prostatic hyperplasia tissue. Zinc, copper, iron, calcium and selenium were analysed in histologically proven 15 prostate cancer tissues and 15 benign prostatic hyperplasia tissues using atomic absorption spectrophotometer. Unpaired two tailed t test/Mann–Whitney U test and Pearson correlation coefficient were used to compare the level of trace elements, elemental ratios and their interrelations. As compared to benign prostatic tissue, malignant prostatic tissue had significantly lower selenium (p = 0.038) and zinc (p = 0.043) concentrations, a lower zinc/iron ratio (p = 0.04) and positive correlation of selenium with zinc (r = 0.71, p = 0.02) and iron (r = 0.76, p = 0.009). Considerably divergent interrelationship of elements and elemental ratios in prostate cancer versus benign prostatic hyperplasia was noted. Understanding of differential elemental changes and their interdependence may be useful in defining the complex metabolic alterations in prostate carcinogenesis with potential for development of element based newer diagnostic, preventive and therapeutic strategies. Further studies may be needed to elucidate this complex relationship between trace elements and prostate carcinogenesis.  相似文献   

Cardioprotective activity of synthetic guggulsterone (E and Z-isomers) in isoproterenol induced myocardial ischemia in rats: A comparative study     

Ramesh Chander  Farhan Rizvi  A. K Khanna  Ram Pratap 《Indian journal of clinical biochemistry : IJCB》2003,18(2):71-79

Guggulsterone, a mixture of cis (E) and trans (Z) isomers (7∶3 w/w) was synthesized from 16-DPA. The isomers were separated by column chromatography and evaluated for cardioprotective and antioxidant activities. Myocardial necrosis induced by isoproterenol in rats caused marked increase in serum creatine phosphokinase and glutamate pyruvate transaminase. Simultaneously in ischemic heart, phospholipase, xanthine oxidase and lipid peroxides were enhanced following depletion of glycogen, phospholipids and cholesterol. Treatment with guggulsterone and its both isomers at the dose of 50 mg/kg po., significantly protected cardiac damage as assessed by the reversal of blood and heart biochemical parameters in ischemic rats. The cardioprotective activity of guggulsterone and of both the isomers were compared with that of gemfibrozil at the same doses. Guggulsterone and both the isomers at tested concentrations (5–20mM) inhibited oxidative degradation of lipids in human low-density lipoprotein and rat liver microsomes induced by metal ionsin vitro. The drug counteracted against the generation of superoxide anions (O₂) and hydroxyl radicals (OH⁻) in non-enzymic test systems. It is suggested that cardioprotective and antioxidant activities of synthetic guggulsterone and guggulsterone obtained from gum resinCommiphora mukul that contains isomers E & Z in the ratio of 46∶54w/w are the same.  相似文献   

Role of MRI in medical diagnostics     

Rashmi Rao  Ramesh Venkatesan  Sairam Geethanath 《Resonance》2015,20(11):1003-1011

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Perception of teaching competencies by administrators,faculty and students of Indian agricultural universities: an assessment of faculty training needs     

P. Ramesh  D. Thammi Raju  K. M. Reddy  P. Krishnan  Amit Biswas  T. Umamaheshwari 《The Journal of Agricultural Education and Extension》2019,25(4):337-359

Purpose: The study aims at finding out relevance and knowledge levels of selected teaching competencies as perceived by educational administrators, faculty and students, in order to assess the training needs of faculty of agricultural universities.

Methodology: Relevance and knowledge levels were tested through a teaching competency questionnaire developed and run on 292 respondents fitting into administrator, faculty and student groups. Needs Assessment Model by Borich [1980. “A Needs Assessment Model for Conducting Follow-up Studies.” Journal of Teacher Education 31 (3): 39–42] is used to identify training needs.

Findings: Results indicate differences in perceptions among groups towards teaching competencies. Prioritized training needs were identified which provide the content and direction for the development of faculty in-service educational programmes.

Practical implications: Faculty of agricultural universities need periodic in-service training programmes in order to improve their teaching competencies so that they become effective and competent teachers in the present educational environment.

Theoretical implications: The statistically validated methodological framework provides for capturing the perception of all stakeholders on the teaching competencies among the faculty members of Agricultural Universities in India, and offers a scope for scaling up the study for similar educational setting in the region.

Originality/value: The perception of students and administrators was also considered along with the self-perception of faculty about the relevance and knowledge levels of teaching competencies.  相似文献   

Plant-fungus marriages     

Ramesh Maheshwari 《Resonance》2006,11(4):33-44

The roots of most land plants establish intimate associations with soil fungi. This association of plant roots with fungi is termed mycorrhiza. It is a marriage between two highly dissimilar organisms based on mutual exchange of nutrients: while the plants provide photosynthetically made carbon compounds to the fungi, the fungi in turn provide to the plants nitrogen and phosphorus scavenged from the breakdown of organic polymers in natural substrates. This mutually beneficial partnership makes possible the green cover on Earth and of all life, ultimately, dependent on plants.  相似文献   

Children's conceptions of probability — A psychological and pedagogical review     

Anne S. Hawkins  Ramesh Kapadia 《Educational Studies in Mathematics》1984,15(4):349-377

This paper identifies a number of key questions concerning children's understanding of probability. A selection of psychological and pedagogical research studies is evaluated. Attention is drawn to the various definitions of probability assumed by their authors, indicating the relevance of these to an understanding and synthesis of the reported findings. The possibility of basing relevant classroom practice on subjective rather than theoretical concepts of probability is discussed.  相似文献   

A number-theoretic game     

Prithvi Ramesh 《Resonance》2003,8(1):84-88

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Detecting Differential Item Functioning Using Logistic Regression Procedures   总被引：2，自引：0，他引：2  

Hariharan Swaminathan  H. Jane Rogers 《Journal of Educational Measurement》1990,27(4):361-370

A logistic regression model for characterizing differential item functioning (DIF) between two groups is presented. A distinction is drawn between uniform and nonuniform DIF in terms of the parameters of the model. A statistic for testing the hypothesis of no DIF is developed. Through simulation studies, it is shown that the logistic regression procedure is more powerful than the Mantel-Haenszel procedure for detecting nonuniform DIF and as powerful in detecting uniform DIF.  相似文献