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1.
The aims of this study were to assess the reliability and validity of three methods of bioelectrical impedance analysis (based on induction between the hand and foot, between one foot and the other foot and between one hand and the other hand) and the skinfold method, and to construct prediction equations for total body density by examining cross-validity in young Japanese adult males. The participants were 50 Japanese males aged 18-27 years (height 1.72 +/- 0.06 m, body mass 64.9 +/- 9.0 kg; mean +/- s), each of whom was measured twice using each of the four methods. Relative body fat based on underwater weighing was used as the criterion for validity. To construct prediction equations for body density, we used multiple regression analysis, whereby all possible combinations were examined. The reliability of all three bioelectrical impedance methods was high (R = 0.999). Three new prediction equations were constructed for the hand-foot method, foot-foot method and skinfold method. The cross-validity of the equations was guaranteed. The relative body fat calculated using the new equations did not differ from that based on the underwater weighing method.  相似文献   
2.
The purpose of this study was to investigate differences in the support leg joint moment and moment power between side-step (SS) and cross-step (CS) cutting techniques with a prescribed 90° cutting angle. Ground reaction forces (1,000 Hz) and three-dimensional kinematics (250 Hz) of SS and CS cutting techniques were collected from 20 male college athletes. Normalised peak knee extension moment was larger in the SS technique than in the CS technique (0.40 ± 0.10 in SS; 0.26 ± 0.08 in CS). In the SS technique, the knee extensors ( ? 0.10 ± 0.06 in SS; ? 0.02 ± 0.04 in CS) and ankle plantarflexors ( ? 0.12 ± 0.05 in SS; ? 0.07 ± 0.03 in CS) did significantly more negative work (normalised). The direction change angle (40.5 ± 8.7° in SS; 33.0 ± 6.8° in CS) and the decrease in horizontal velocity of the centre of mass ( ? 0.63 ± 0.23 m/s in SS; ? 0.31 ± 0.23 m/s in CS) were significantly larger in the SS technique. These results suggest that the SS technique is an effective means of changing running direction at the expense of velocity of the centre of mass and that the CS technique is better for minimising the reduction in horizontal velocity of the centre of mass.  相似文献   
3.
Accurate measurement of head volume is indispensable for precise assessments of body composition determined by hydrostatic weighing without head submersion. The purpose of this study was to establish a prediction equation for head volume measured by the immersion method from multiple regression analysis using head parameters (head circumference, head length, head breadth, neck girth and head thickness) as independent variables. The participants were 106 Japanese young adults (55 males and 51 females) aged 17-27 years. Intra-class correlation coefficients (ICCs) for each head parameter and head volume in males and females were very high (ICC = 0.993-0.999, 0.992-0.998). Head circumference was closely related to head volume measured by the immersion method (r = 0.719, 0.861, P < 0.05), and was the most important parameter for the prediction equation in both sexes. Head breadth was related poorly (r = 0.475, 0.500, P < 0.05) and showed a small individual difference. It was, therefore, excluded from the independent variables. The prediction equation for males was predicted head volume = 122.10X1 + 106.19X3 + 37.16X4 - 89.46X5 - 4754.93, R = 0.909, SEE = 121.75 ml, and that for females was predicted head volume = 213.83X1 + 45.24X3 + 36.85X4 - 74.34X5 - 8912.43, R = 0.913, SEE = 136.26 ml (where X1 = head circumference, X3 = head length, X4 = neck girth, X5 = head thickness, and SEE = standard error of the estimate). The limits of agreement for predicted and measured head volume were -234.5 to 234.1 ml for males, and -261.0 to 261.0 ml for females. In cross-validation groups of both sexes, there were no significant differences between measured head volume and predicted head volume. The correlation coefficients between measured head volume and predicted head volume in males and females were 0.894 and 0.908, respectively. The predicted head volume from prediction equations was considered to have high reliability and validity.  相似文献   
4.
This study proposes a new methodological approach to evaluate students’ knowledge-building discourse. First, we discuss the knowledge–creation metaphor of learning. In the metaphor, theories mention that learners should consider their collective knowledge objects or artifacts that materialize as a result of their collaborative discourse. Second, we argue the necessity of developing new analytics to evaluate student learning. We describe how students’ ideas and their conceptual artifacts can be examined in discourse analysis. Third, we demonstrate the application of our analytics to real discourse data. We conducted a new type of social network analysis of discourse to examine how students continuously improve their ideas. Further, we conducted another network analysis of discourse, called the Epistemic Network Analysis, by coding students’ epistemic actions as conceptual artifacts to create and examine their ideas.  相似文献   
5.
There is a large residual volume (RV) error when assessing percent body fat by means of hydrostatic weighing. It has generally been measured before hydrostatic weighing. However, an individual's maximal exhalations on land and in the water may not be identical. The aims of this study were to compare residual volumes and vital capacities on land and when immersed to the neck in water, and to examine the influence of the measurement error on percent body fat. The participants were 20 healthy Japanese males and 20 healthy Japanese females. To assess the influence of the RV error on percent body fat in both conditions and to evaluate the cross-validity of the prediction equation, another 20 males and 20 females were measured using hydrostatic weighing. Residual volume was measured on land and in the water using a nitrogen wash-out technique based on an open-circuit approach. In water, residual volume was measured with the participant sitting on a chair while the whole body, except the head, was submerged . The trial-to-trial reliabilities of residual volume in both conditions were very good (intraclass correlation coefficient > 0.98). Although residual volume measured under the two conditions did not agree completely, they showed a high correlation (males: 0.880; females: 0.853; P < 0.05). The limits of agreement for residual volumes in both conditions using Bland-Altman plots were -0.430 to 0.508 litres. This range was larger than the trial-to-trial error of residual volume on land (-0.260 to 0.304 litres). Moreover, the relationship between percent body fat computed using residual volume measured in both conditions was very good for both sexes (males: r = 0.902; females: r = 0.869, P < 0.0001), and the errors were approximately -6 to 4% (limits of agreement for percent body fat: -3.4 to 2.2% for males; -6.3 to 4.4% for females). We conclude that if these errors are of no importance, residual volume measured on land can be used when assessing body composition.  相似文献   
6.
Understanding biomolecular gradients and their role in biological processes is essential for fully comprehending the underlying mechanisms of cells in living tissue. Conventional in vitro gradient-generating methods are unpredictable and difficult to characterize, owing to temporal and spatial fluctuations. The field of microfluidics enables complex user-defined gradients to be generated based on a detailed understanding of fluidic behavior at the μm-scale. By using microfluidic gradients created by flow, it is possible to develop rapid and dynamic stepwise concentration gradients. However, cells exposed to stepwise gradients can be perturbed by signals from neighboring cells exposed to another concentration. Hence, there is a need for a device that generates a stepwise gradient at discrete and isolated locations. Here, we present a microfluidic device for generating a stepwise concentration gradient, which utilizes a microwell slide''s pre-defined compartmentalized structure to physically separate different reagent concentrations. The gradient was generated due to flow resistance in the microchannel configuration of the device, which was designed using hydraulic analogy and theoretically verified by computational fluidic dynamics simulations. The device had two reagent channels and two dilutant channels, leading to eight chambers, each containing 4 microwells. A dose-dependency assay was performed using bovine aortic endothelial cells treated with saponin. High reproducibility between experiments was confirmed by evaluating the number of living cells in a live-dead assay. Our device generates a fully mixed fluid profile using a simple microchannel configuration and could be used in various gradient studies, e.g., screening for cytostatics or antibiotics.  相似文献   
7.
Abstract

We previously proposed two predictive equations of visceral fat area applicable in a field setting (Demura & Sato, 2007a Demura, S. and Sato, S. 2007a. Prediction of visceral fat area at the umbilicus level using fat mass of the trunk: The validity of bioelectrical impedance analysis. Journal of Sports Sciences, 25: 823833. [Taylor & Francis Online], [Web of Science ®] [Google Scholar], 2007b Demura, S. and Sato, S. 2007b. Prediction of visceral fat area in Japanese adults: Proposal of prediction method applicable in a field setting. European Journal of Clinical Nutrition, 61: 727735. [Crossref], [PubMed], [Web of Science ®] [Google Scholar]). One uses the fat mass of the trunk measured by bioelectrical impedance as the main predictor (equation 1) and the other uses internal fat mass estimated from several anthropometric variables (equation 2). In this study, we examined the inter- and intra-individual accuracy of estimated values using these equations after 8 weeks of exercise training. Participants were 42 Japanese adults aged 42.7 ± 10.3 years (22 males, 20 females). Visceral fat area, body composition, and blood biochemistry were measured at baseline and after 8 weeks. There were no significant differences in reference visceral fat area measured by computed tomography and visceral fat area predicted by the equations either at baseline or after 8 weeks, and the values were highly consistent (equation 1: baseline R 2 = 0.829, after R 2 = 0.860; equation 2: baseline R 2 = 0.832; after R 2 = 0.850). No significant relationship was observed between the reference and change in visceral fat area for equation 1 (males: r = 0.272, P > 0.05; females: r = 0.428, P > 0.05), but there was a significant relationship for females with equation 2 (males: r = 0.279, P > 0.05; females: r = 0.474, P <0.05). Our findings indicate that these equations have high inter-individual consistency but low intra-individual consistency with the reference and are of limited use for the longitudinal evaluation of visceral fat area.  相似文献   
8.
The aims of this study were to assess the validity and reliability of body volume and percent body fat determined by sulphur hexafluoride dilution, using underwater weighing as the criterion method, and to determine the influence of the magnitude of body volume. Thirty-one healthy Japanese individuals aged 18-27 years (16 males: height 1.70 +/- 0.06 m, mass 64.8 +/- 7.7 kg; 15 females: height 1.60 +/- 0.05 m, mass 55.2 +/- 6.2 kg; mean +/- s) participated in the study. Sulphur hexafluoride dilution measures the concentration of sulphur hexafluoride gas in the chamber (BSF-200, Shimazu Corp.). Underwater weighing was performed five times using a weight scale (AD-6204, A&D) after residual volume had been determined (System9, Minato Medical Corp.). There were no significant differences in the mean between two trials for body volume, body density or percent body fat determined by sulphur hexafluoride dilution. The intra-class correlation coefficient of these variables ranged from 0.985 to 0.999. The results suggest that sulphur hexafluoride dilution is a reliable method for assessing body composition. There was no significant difference in body volume or percent body fat between sulphur hexafluoride dilution (males: 61.3 +/- 7.6 litres, 18.4 +/- 6.7%; females: 52.8 +/- 6.9 litres, 21.0 +/- 8.9%) and underwater weighing (males: 60.6 +/- 7.0 litres, 15.6 +/- 3.5%; females: 53.0 +/- 6.5 litres, 23.7 +/- 6.1%) and there was a high correlation between the two (r = 0.997, P < 0.05). A Bland-Altman plot of the difference between percent body fat estimated by underwater weighing and sulphur hexafluoride dilution versus average percent body fat by the two methods showed no systematic difference (mean difference = -0.12 +/- 6.6 kg). The upper and lower limits of agreement were 13.2% and -13.4%, respectively. Determination by sulphur hexafluoride dilution resulted in both over- and underestimations in body volume and the difference between the two body volumes (determined by underwater weighing and by sulphur hexafluoride dilution) was inversely proportional to the mean body volume by the two methods. This suggests that improvements need to be made to the device or to the technique to maintain a constant volume of sulphur hexafluoride in the chamber.  相似文献   
9.
This research reveals an unexplored aspect of Argentina’s sport policy-making process during the first three decades of the twentieth century. Contrary to the common assumption that little attention was paid to sport by early twentieth-century politicians, several bills proposing institutional support for the promotion and organization of national sport were presented in the Argentinean legislature. In order to support these proposals, many legislators resorted to medical and physiological discourse as the most important legitimizing force. Poverty, poor hygiene, and epidemic diseases, generated by rapid modernization and urbanization, urged some turn-of-the-century Argentine political elites to attempt a degree of social intervention within the general framework of the liberal-conservative order, as a way to counteract these evils and further advance the national progress enjoyed since the nineteenth century. In this context, sport was ardently advocated by some politicians as a means to raise scientifically the physical and moral constitution of the ‘Argentinean race’.  相似文献   
10.
Accurate measurement of head volume is indispensable for precise assessments of body composition determined by hydrostatic weighing without head submersion. The purpose of this study was to establish a prediction equation for head volume measured by the immersion method from multiple regression analysis using head parameters (head circumference, head length, head breadth, neck girth and head thickness) as independent variables. The participants were 106 Japanese young adults (55 males and 51 females) aged 17?–?27 years. Intra-class correlation coefficients (ICCs) for each head parameter and head volume in males and females were very high (ICC = 0.993?–?0.999, 0.992?–?0.998). Head circumference was closely related to head volume measured by the immersion method (r = 0.719, 0.861, P <?0.05), and was the most important parameter for the prediction equation in both sexes. Head breadth was related poorly (r = 0.475, 0.500, P <?0.05) and showed a small individual difference. It was, therefore, excluded from the independent variables. The prediction equation for males was predicted head volume = 122.10X 1 + 106.19X 3 + 37.16X 4 - 89.46X 5 - 4754.93, R = 0.909, SEE = 121.75?ml, and that for females was predicted head volume = 213.83X 1 + 45.24X 3 + 36.85X 4 - 74.34X 5 - 8912.43, R = 0.913, SEE = 136.26?ml (where X 1 = head circumference, X 3 = head length, X 4 = neck girth, X 5 = head thickness, and SEE = standard error of the estimate). The limits of agreement for predicted and measured head volume were –?234.5 to 234.1?ml for males, and ??261.0 to 261.0?ml for females. In cross-validation groups of both sexes, there were no significant differences between measured head volume and predicted head volume. The correlation coefficients between measured head volume and predicted head volume in males and females were 0.894 and 0.908, respectively. The predicted head volume from prediction equations was considered to have high reliability and validity.  相似文献   
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