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Eric R. Muoz Jaclyn B. Caccese Brittany E. Wilson Kyle T. Shuler Fernando V. Santos Carolina T. Cabn John J. Jeka Dianne Langford Matthew B. Hudson 《运动与健康科学(英文)》2021,10(2):122
BackgroundConsidering the potential cumulative effects of repetitive head impact (HI) exposure, we need sensitive biomarkers to track short- and long-term effects. Circulating small extracellular vesicles (sEVs) (<200 nm) traffic biological molecules throughout the body and may have diagnostic value as biomarkers for disease. The purpose of this study was to identify the microRNA (miRNA) profile in circulating sEVs derived from human plasma following repetitive HI exposure.MethodsHealthy adult (aged 18–35 years) soccer players were randomly assigned to one of 3 groups: the HI group performed 10 standing headers, the leg impact group performed 10 soccer ball trapping maneuvers over 10 min, and the control group did not participate in any soccer drills. Plasma was collected before testing and 24 h afterward, and sEVs were isolated and characterized via nanoparticle tracking analysis. Next-generation sequencing was utilized to identify candidate miRNAs isolated from sEVs, and candidate microRNAs were analyzed via quantitative polymerase chain reaction. In silico target prediction was performed using TargetScan (Version 7.0; targetscan.org) and miRWalk (http://mirwalk.umm.uni-heidelberg.de/) programs, and target validation was performed using luciferase reporter vectors with a miR-7844-5p mimic in human embryonic kidney (HEK) 293T/17 cells.ResultsPlasma sEV concentration and size were not affected across time and group following repetitive HI exposure. After 24 h, the HI read count from next-generation sequencing showed a 4-fold or greater increase in miR-92b-5p, miR-423-5p, and miR-24-3p and a 3-fold or greater decrease in miR-7844-5p, miR-144-5p, miR-221-5p, and miR-22-3p. Analysis of quantitative polymerase chain reaction revealed that leg impact did not alter the candidate miRNA levels. To our knowledge, miR-7844-5p is a previously unknown miRNA. We identified 8 miR-7844-5p mRNA targets: protein phosphatase 1 regulatory inhibitor subunit 1B (PPP1R1B), LIM and senescent cell antigen-like domains 1 (LIMS1), autophagy-related 12 (ATG12), microtubule-associated protein 1 light chain 3 beta (MAP1LC3B), integrin subunit alpha-1 (ITGA1), mitogen-activated protein kinase 1 (MAPK1), glycogen synthase kinase 3β (GSK3β), and mitogen-activated protein kinase 8 (MAPK8).ConclusionCollectively, these data indicate repetitive HI exposure alters plasma sEV miRNA content, but not sEV size or number. Furthermore, for the first time we demonstrate that previously unknown miR-7844-5p targets mRNAs known to be involved in mitochondrial apoptosis, autophagy regulation, mood disorders, and neurodegenerative disease. 相似文献
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Samuel T. Orange Phil Marshall Leigh A. Madden Rebecca V. Vince 《Journal of sports sciences》2019,37(11):1227-1234
This study examined the relationship between sit-to-stand (STS) power and physical function in adults with severe obesity. Thirty-eight adults (age: 44 ± 12 years; body mass index [BMI]: 45.2 ± 7.8 kg/m2) completed evaluations of STS power, strength and functional performance. STS power was measured with a wearable inertial sensor, strength was assessed with the isometric mid-thigh pull, and function was measured with the timed up-and-go (TUG), six-minute walk test (6MWT) and 30-s chair STS. Power and strength (normalised to body mass) entered regression models in addition to age, gender, BMI and physical activity (daily step count). Power displayed large univariate associations with TUG (r = 0.50) and 30-s chair STS (r = 0.67), and a moderate association with 6MWT (r = 0.49). Forward stepwise regression revealed that power independently contributed to TUG (β = ?0.40, p = 0.010), 30-s chair STS (β = 0.67, p < 0.001) and 6MWT performance (β = 0.27, p = 0.007). Power also appeared to be a superior determinant of function compared with strength. Power generated via the STS transfer largely underpins the ability to perform functional tasks in adults with severe obesity, although intervention studies are required to investigate a potentially causal relationship. 相似文献
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ABSTRACTThe preservation of artefacts in museum collections is profoundly affected by fluctuations in temperature and, especially, relative humidity (RH). Since the late nineteenth century, many studies have been carried out on the best way to control hygrothermal conditions. In old buildings located in maritime temperate climate zones (such as Portugal) with strong thermal inertia, and which have low ventilation rate (relative to the volume and number of visitors), daily and seasonal hygroscopic inertia may help to assure the maintenance of RH stabilization conditions. The use of expensive active systems may be minimized through the passive behaviour of internal finishing building materials. This work presents the results of an experimental laboratory study conducted in a flow chamber to demonstrate the enormous potential of hygroscopic materials in stabilizing interior relative humidity. Based in these results and in-situ monitoring in a museum housed in a building, located in Porto, with a typical construction of the 1950s (granite masonry and reinforced concrete slabs), a numerical analysis was done to quantify the influence of hygroscopic materials in stabilizing the interior relative humidity. 相似文献
5.
Liezel Hurter Alex V. Rowlands Stuart J. Fairclough Karl C. Gibbon Zoe R. Knowles Lorna A. Porcellato 《Journal of sports sciences》2019,37(16):1910-1918
This study aimed to validate the Sedentary Sphere posture classification method from wrist-worn accelerometers in children. Twenty-seven 9–10-year-old children wore ActiGraph GT9X (AG) and GENEActiv (GA) accelerometers on both wrists, and activPAL on the thigh while completing prescribed activities: five sedentary activities, standing with a phone, walking (criterion for all 7: observation) and 10-min free-living play (criterion: activPAL). In an independent sample, 21 children wore AG and GA accelerometers on the non-dominant wrist and activPAL for two days of free-living. Per cent accuracy, pairwise 95% equivalence tests (±10% equivalence zone) and intra-class correlation coefficients (ICC) analyses were completed. Accuracy was similar, for prescribed activities irrespective of brand (non-dominant wrist: 77–78%; dominant wrist: 79%). Posture estimates were equivalent between wrists within brand (±6%, ICC > 0.81, lower 95% CI ≥ 0.75), between brands worn on the same wrist (±5%, ICC ≥ 0.84, lower 95% CI ≥ 0.80) and between brands worn on opposing wrists (±6%, ICC ≥ 0.78, lower 95% CI ≥ 0.72). Agreement with activPAL during free-living was 77%, but sedentary time was underestimated by 7% (GA) and 10% (AG). The Sedentary Sphere can be used to classify posture from wrist-worn AG and GA accelerometers for group-level estimates in children, but future work is needed to improve the algorithm for better individual-level results. 相似文献
6.
Pauliina Husu Jaana Suni Kari Tokola Henri Vähä-Ypyä Heli Valkeinen Tomi Mäki-Opas 《Journal of sports sciences》2019,37(16):1840-1848
This cross-sectional study analysed the association of sedentary behaviour (SB) and standing with waist circumference. Finnish adults aged 30-75 years from a sub-sample of population-based Health 2011 Study used a hip-worn tri-axial accelerometer (Hookie AM20, Traxmeet, Ltd, Espoo, Finland) for seven days. Those 1405 participants (57% women) who used accelerometer at least four days, minimum of 10 hours/day, were included. SB and standing were analysed in 6s epochs using validated algorithm. Daily total time, daily number and accumulated time from bouts (30s–5min,30s–10min,30s–15min,30s 30min,>5min,>10min,>15min, >30min) as well as daily number of sit-to-stand transitions were determined. Waist circumference (cm) was measured in standardized way. Participants’ mean age was 52 years (SD 12.2). Mean waist circumference was 97cm (SD 12.3) in men and 87cm (SD 12.7) in women. According to multivariable stepwise linear regression analysis, sit-to-stand transitions [standardised regression coefficient (β)= -0.14, 95%CI -0.20 to -0.09], standing bouts of 30s–5min (β= -0.21, 95%CI -0.26 to -0.15) and number of 30s-30min SB bouts (β=0.12, 95%CI 0.06 to 0.17) were most strongly associated with waist circumference. Besides assessing total SB time, future studies should assess also different bout lengths of SB and sit-to-stand transitions and standing times should be assessed separately. 相似文献
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Pedro L. Valenzuela Michael McGuigan Guillermo Sánchez-Martínez Elaia Torrontegi Javier Vázquez-Carrión Zigor Montalvo 《Journal of sports sciences》2020,38(19):2273-2278
ABSTRACT The present study aimed to provide reference values for lower-limb muscle power assessed during the incremental jump squat (JS) test in elite athletes (i.e., professional athletes competing at international level). We pooled data from all JS tests performed by elite athletes of different sports in two high-performance centres between 2015 and 2019, and computed reference values (i.e., terciles) for mean power (MP), mean propulsive power (MPP), and peak power (PP). Reference values were obtained from 684 elite athletes (458 male and 226 female) of 16 different sports (boxing, judo, karate, fencing, taekwondo, wrestling, basketball, soccer, futsal, handball, rugby union, badminton, tennis, long distance running, triathlon, and sprinting). Significant differences (p < 0.001) were found between male and female athletes for MP (7.47 ± 1.93 and 6.15 ± 1.68 W·Kg?1, respectively), MPP (10.50 ± 2.75 and 8.63 ± 2.43 W·Kg?1), and PP (23.64 ± 6.12 and 19.35 ± 5.49 W·Kg?1). However, the velocity at which these power measures was attained seemed to be independent of sex (~0.95, 1.00 and 2.00 m·s?1 for mean, mean propulsive, and peak velocity, respectively) and homogeneous across different sport disciplines (coefficient of variation <10%). These data can be used to classify athletes’ power capabilities, and the optimum velocity ranges provided here could be useful for training purposes. 相似文献
9.
Luana L. Cabral Fábio Y. Nakamura Joice M. F. Stefanello Luiz C. V. Pessoa Bruno P. C. Smirmaul Gleber Pereira 《Measurement in physical education and exercise science》2020,24(2):103-114
ABSTRACTThe aims of this study were to perform the cross-cultural adaptation of the Borg Rating of Perceived Exertion (RPE) 6–20 Scale to Brazilian Portuguese language and to start testing its validity and reliability. After performing the cross-cultural adaptation of the Scale, concurrent and discriminative validity, and reliability were determined on a treadmill in young (18–30 years) and older adult women (60–75 years). Considering young and older adult women, RPE presented high and moderate positive correlation with heart rate and high and low positive correlation with oxygen consumption, respectively. Older adult women presented RPE (11 ± 2) significantly higher than young ones (8 ± 1) exercising at the same absolute intensity. Intraclass coefficient correlation was excellent for RPE to young and older adult women. The Scale presented concurrent validity only to young adult women, whereas it presented discriminative validity between such groups of women. Moreover, the scale is reliable to young and older adult women. 相似文献
10.
V. V. Sogrin 《Russian Education & Society》2014,56(5):15-40
The teaching and public dissemination of Russian history in post-Soviet historiography has been shaped by a variety of approaches, including state-sponsored interpretations, views expressed in mass culture, and the work of academic historians. 相似文献