排序方式： 共有16条查询结果，搜索用时 15 毫秒
1.
Aarash Sofla Bojana Cirkovic Anne Hsieh Jason W. Miklas Nenad Filipovic Milica Radisic 《Biomicrofluidics》2013,7(1)
The majority of available cardiomyocyte markers are intercellular proteins, limiting our ability to enrich live cardiomyocytes from heterogeneous cell preparations in the absence of genetic labeling. Here, we describe enrichment of live cardiomyocytes from the hearts of adult mice in a label-free microfluidic approach. The separation device consisted of a vertical column (15 mm long, 700 μm diameter), placed between permanent magnets resulting in a field strength of 1.23 T. To concentrate the field at the column wall, the column was wrapped with 69 μm diameter nickel wire. Before passing the cells through the column, the cardiomyocytes in the cell suspension had been rendered paramagnetic by treatment of the adult mouse heart cell preparation with sodium nitrite (2.5 mM) for 20 min on ice. The cell suspension was loaded into the vertical column from the top and upon settling, the non-myocytes were removed by the upward flow from the column. The cardiomyocytes were then collected from the column by applying a higher flow rate (144 μl/min). We found that by applying a separation flow rate of 4.2 μl/min in the first step, we can enrich live adult cardiomyocytes to 93% ± 2% in a label-free manner. The cardiomyocytes maintained viability immediately after separation and upon 24 h in culture. 相似文献
2.
Hemolysis of erythrocytes from human and porcine blood was studied at different calcium chloride (CaCl2) concentrations (0.04–1.83 mM) and at a constant bilirubin concentration (72 mM). Although, human erythrocytes showed significant
hemolysis (64%) at the highest CaCl2 concentration (1.83 mM) used in this study, remarkable resistance to this phenomenon was observed with porcine erythrocytes
as only 11% hemolysis was observed. A similar pattern in the behavior of both human and porcine erythrocytes was observed
when parameter such as bilirubin concentration or time of incubation with bilirubin was varied. Other divalent cations such
as Mn2+, Ba2+ and Mg2+ were either least effective or ineffective in inducing hemolysis in presence of bilirubin. Serum albumin played a protective
role in this phenomenon in a concentration dependent manner, as no hemolysis was observed at a bilirubin/albumin molar ratio
of 1:1 or less. Differences in the structural make-up of proteins and lipids in the erythrocyte membranes of the two species
may account for their different behavior. 相似文献
3.
Marija Brajdić Vuković Bojana Vignjević Korotaj Bojana Ćulum Ilić 《European Journal of Education》2020,55(4):542-559
Academic systems are undergoing changes in which the social organisation of research as well as patterns of scientific productivity in the humanities and social sciences progressively resemble those in hard sciences. The hard and soft sciences are increasingly converging. This development can be observed in (a) publishing patterns, (b) the division of research, and (c) the internationalisation of research. This study explored the extent to which these changes occurring in academic systems in terms of a transformation of disciplinary practices are also becoming a trend in transitional post-socialist countries. We used Croatia as a case of a post socialist transitional context and compared it to Slovenia, a country with a similar past but somewhat different science policies and strategies. The results point to increasing convergence in some soft disciplines, visible in projectification and internationalisation of academic work as well as a significant change in the publishing patterns. 相似文献
4.
Jelena Teodorović Dejan Stanković Bojana Bodroža Vladeta Milin Ivana Đerić 《Educational Assessment, Evaluation and Accountability》2016,28(4):347-375
Numerous educational reforms have been initiated in Serbia since 2000. The aim of this study was to examine education policymaking in Serbia in order to provide policymakers and stakeholders with recommendations on how to minimize pitfalls and increase success of future educational endeavors. In the study, we utilized the education policy cycle heuristic to analyze formation, implementation, and evaluation of three major education policies in Serbia: in-service training of teachers (INSET), school development planning (SDP), and inclusive education (IE). Examining three reforms simultaneously enabled us to identify major characteristics of Serbian policymaking, regardless of the reform content. We also relied on educational change literature, so that we could assess complex contexts of reforms. Finally, considering the importance of school staff during implementation, we examined education policymaking in Serbia through the eyes of over 1,800 teachers, counselors, and principals. The results point out to dissatisfaction of school staff with all aspects of the policymaking in Serbia and with the social and education context. Reforms appear to be undertaken in a similar pattern across different reforms. The findings imply that a thorough examination of both the policymaking in Serbia and communication, organization, and administration within the education system itself is in order. 相似文献
5.
David M. Hoffman Brigida Blasi Bojana Ćulum Žarko Dragšić Amy Ewen Hugo Horta Terhi Nokkala Cecilia Rios-Aguilar 《Higher Education》2014,67(4):473-495
This self-ethnography complements the other articles in this special issue by spotlighting a set of key challenges facing international research teams. The study is focused on the relationship between information and communication technology (ICT)-based collaboration and research team dynamics. Our diverse team, drawn from researchers in five countries and three projects, argues that an ironic casualty of the powerful, global phenomena we study, is a lack of insight into what happens to generic research team dynamics, when groups are ‘stretched’ in terms of geographical distance, generations, cultural beliefs, values and norms, as well as disciplinary/specialist traditions. Good intentions are not sufficient to cope with these challenges. This is because of the emerging complexity inherent in many types of international, interdisciplinary fields of study and the complexity of the career trajectories needed to make these studies a reality. Our study underlines that there are no beliefs, values, norms and practices linked to research team dynamics, that hold across the current territory, generations, disciplines, cultures, organizations and individuals leading and conducting comparative studies—and even less reflection on the implications of this fact. Compounding this lack of awareness is a less-than-perfect understanding of the way in which ICT-based collaboration bears on research team dynamics. We assert that a holistic, critical, long-term approach to emerging insights into the global division of academic labor, serves our field better than folk psychology or the methodological parochialism that sustains convention at the expense of creativity. Careful consideration of emergent processes, relationships and linkages that explain how short-term cooperation—within projects—begins to make sense—over careers—illuminates key focal points, which, in turn qualitatively illuminates the way forward concerning conceptualization and problematization of our practice; and novel methodological routes available for those interested in attaining better outcomes, over the long term. 相似文献
6.
Dejan?Mati? Sladjana?CabriloEmail author Leposava?Grubi?-Ne?i? Bojana?Mili? 《知识管理研究与实践》2017,15(3):431-446
This paper presents a research model aimed to investigate the impact of organizational climate, motivational drivers, and empowering leadership on behavioral intention formation in knowledge sharing. A research model has been developed by integrating several knowledge management studies having in-focus behavioral intention formation in knowledge sharing. The hypotheses are tested on data collected from 873 employees from public and private sector organizations from Serbia’s province of Vojvodina, using partial least square structural modeling technique and SmartPLS software. Findings suggest that organizational climate with prominent dimensions of fairness, innovativeness and affiliation, empowering leadership, sense of self-worth, and altruism significantly influence individuals’ knowledge-sharing attitude, intention, and behavior thus confirming the theory of reasoned action. Results obtained in this research expand and deepen findings reported in previous studies and enable a comprehensive framework for understanding the influence of some organizational variables on employees’ tendencies to be engaged in knowledge sharing. 相似文献
7.
Boh Young Lee 《Early Childhood Education Journal》2017,45(4):521-527
Fifteen toddlers (2- to 3-years old, nine boys and six girls) in a university preschool classroom were observed for 7 months while spending time with books during transition time, between story time and lunch. This qualitative case study investigated the ways that teachers can facilitate toddlers’ reading habits by providing literacy opportunities in unstructured learning environments and explored the contexts in which toddlers engage in independent reading activities. The findings of this study show that (1) toddlers can develop literacy through participating in meaning-making activities with peers while reading books together in unstructured environments and (2) toddlers want to read and choose to read in order to satisfy their curiosity, involvement, and social interchange, just as older children do. 相似文献
8.
9.
Boh Young Lee 《Early Childhood Education Journal》2013,41(1):25-37
The paper examines publication trends in the United States regarding literacy development in toddlerhood from 1990 to 2009, exploring what features or elements of toddlers’ literacy development have been documented, and how they have been documented, over the last 20 years, to indicate areas for further exploration. Articles were carefully selected with predetermined exclusion criteria within five chosen databases that cover professional and scholarly peer-reviewed journals in a variety of areas in education. The selected articles then were analyzed and categorized with predefined categories: publication year, type of article, participants, methodology, research setting, and purpose(s) of study. 相似文献
10.
Boh Young Lee 《Early Childhood Education Journal》2011,39(4):285-290
The purpose of this current study is to build on the previous body of emergent literacy research by investigating the necessity
of assessing book knowledge (e.g., print knowledge, interpretive knowledge, and letter identification) in toddlerhood to set
up successful literacy development by providing independent storybook reading opportunities. This study suggests strategies
that teachers can employ in their toddler classrooms for accomplishing activities such as (i) setting up common and recurring
opportunities to read books independently with toddlers and (ii) informally observing and assessing children’s book knowledge.
Additionally, implications for practice are provided with detailed examples of how toddlers demonstrate their book knowledge
and their understandings about books through independent reading. 相似文献