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Recent years have witnessed a strong trend towards analysis of single-cells. To access and handle single-cells, many new tools are needed and have partly been developed. Here, we present an improved version of a single-cell printer which is able to deliver individual single cells and beads encapsulated in free-flying picoliter droplets at a single-bead efficiency of 96% and with a throughput of more than 10 beads per minute. By integration of acoustophoretic focusing, the cells could be focused in x and y direction. This way, the cells were lined-up in front of a 40 μm nozzle, where they were analyzed individually by an optical system prior to printing. In agreement with acoustic simulations, the focusing of 10 μm beads and Raji cells has been achieved with an efficiency of 99% (beads) and 86% (Raji cells) to a 40 μm wide center region in the 1 mm wide microfluidic channel. This enabled improved optical analysis and reduced bead losses. The loss of beads that ended up in the waste (because printing them as single beads arrangements could not be ensured) was reduced from 52% ± 6% to 28% ± 1%. The piezoelectric transducer employed for cell focusing could be positioned on an outer part of the device, which proves the acoustophoretic focusing to be versatile and adaptable. 相似文献
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András Korcsog András Rohonyi Ferencz Béldi Kálmán Gasztonyi Tibor Meisel 《Higher Education》1977,6(1):1-43
An experiment designed to train engineering students to two levels of terminal achievement within single institutions is reported. The operative engineers (formerly high level technicians) qualify after three years whilst research and development engineers (fully qualified engineers) take five years. Organisational and curricular problems necessarily created by such integrated schemes are described and discussed. Social attitudes in society in general and in the student body in particular make the task of directing students towards the shorter form of training complex and difficult. From the experience gained so far it can confidently be asserted that the experiments in two-level training have been successful.Note by the Editor: This article is an abbreviated version prepared by the Editor of a 90-page UNESCO (copyright) report with the same title, Document ED. 75/WS/64, dated October 1975. We are grateful to the authors and to UNESCO for permission to print this summary of the report. 相似文献
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Martin?VeselyEmail author Thomas?Baron Jean-Yves?Le?Meur Tibor?Simko 《Publishing Research Quarterly》2004,20(1):77-83
Conclusion In this paper we briefly described the current status of research in the field of digital libraries interoperability, mainly
with respect to federated data processing such as permitted within the Open Archive Initiative framework. We then presented
the CERN Document Server Software suite that is a free software package main-tained by CERN providing an online digital library
solution for mid- to large-sized document repositories. The set of CDSware modules was described and the differ-ences with
other existing tools briefly mentioned. Finally we have mentioned a typical usage statistics illustrating the scope of grey
literature and networked con-ditions of the CERN Document Server.
We pointed out that the current trend in grey literature management goes toward institutional repositories build upon the
distributed and federated model. Further research is going on within the CDS collaboration in this area, focused particularly
on (i) semantic interoperability using ontologies in distributed and federated data processing and (ii) ranking technologies
when searching grey literature in OAI compliant repositories. 相似文献
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The article provides an overview of the development of teaching science in Hungary during both the time of the dual monarchy and the newly established independent Hungary after 1920. The integration of Hungary into the Austro-Hungarian Monarchy (1867–1918) strengthened the effect of German speaking European science, the results of which were quickly channelled into the Hungarian school system at all levels. The Hungarian Academy as well as the University of Budapest (today Eötvös Loránd University) played a leading role in the ?nationalization” of European science in the educational system. Scientific developments in Hungary strengthened the position of rational and secular thinking in a highly religious society and contributed to the erosion of the mental power of the church tradition, particularly that of the Roman Catholic Church. Toward World War I, influenced by the Protestant Churches, the Jewish tradition, and agnosticism, the public picture of science became more international, occasionally ready to consider challenges of the accepted world view, and sometimes less dogmatic. Leading Hungarian figures with an international reputation who played a decisive role in making science part of Hungarian thinking included the physicists Baron Loránd Eötvös and Sándor Mikola, the mathematicians László Rácz and George Pólya as well as a host of others in related fields. Emigration, mostly Jewish, after World War I, contributed to the curtailment of efforts to teach science effectively as some of the best people left Hungary for, mostly, Germany, Britain, and the United States. However, the interwar school system, the Hungarian version of the German Gymnasium, continued to disseminate scientific thought in Hungarian education. Much of the information was foreign and appeared simply in translation—but an impressive array of indigeneous scientific results paved the way to a larger educated middle class then in the making. 相似文献
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Label-free isolation of single cells is essential for the growing field of single-cell analysis. Here, we present a device which prints single living cells encapsulated in free-flying picoliter droplets. It combines inkjet printing and impedance flow cytometry. Droplet volume can be controlled in the range of 500 pl–800 pl by piezo actuator displacement. Two sets of parallel facing electrodes in a 50 μm × 55 μm channel are applied to measure the presence and velocity of a single cell in real-time. Polystyrene beads with <5% variation in diameter generated signal variations of 12%–17% coefficients of variation. Single bead efficiency (i.e., printing events with single beads vs. total number of printing events) was 73% ± 11% at a throughput of approximately 9 events/min. Viability of printed HeLa cells and human primary fibroblasts was demonstrated by culturing cells for at least eight days. 相似文献
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