Structural and dynamic properties of linker histone H1 binding to DNA     

Dootz R  Toma AC  Pfohl T 《Biomicrofluidics》2011,5(2):24104

Found in all eukaryotic cells, linker histones H1 are known to bind to and rearrange nucleosomal linker DNA. In vitro, the fundamental nature of H1∕DNA interactions has attracted wide interest among research communities-from biologists to physicists. Hence, H1∕DNA binding processes and structural and dynamical information about these self-assemblies are of broad importance. Targeting a quantitative understanding of H1 induced DNA compaction mechanisms, our strategy is based on using small-angle x-ray microdiffraction in combination with microfluidics. The usage of microfluidic hydrodynamic focusing devices facilitates a microscale control of these self-assembly processes, which cannot be achieved using conventional bulk setups. In addition, the method enables time-resolved access to structure formation in situ, in particular, to transient intermediate states. The observed time dependent structure evolution shows that the H1∕DNA interaction can be described as a two-step process: an initial unspecific binding of H1 to DNA is followed by a rearrangement of molecules within the formed assemblies. The second step is most likely induced by interactions between the DNA and the H1's charged side chains. This leads to an increase in lattice spacing within the DNA∕protein assembly and induces a decrease in the correlation length of the mesophases, probably due to a local bending of the DNA.  相似文献   

Student and faculty perceptions of service quality: the moderating role of the institutional aspects     

Ines Dužević  Anita Čeh Časni 《Higher Education》2015,70(3):567-584

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Work-related burnout syndrome in special education teachers working with children with developmental disorders – possible correlations with some socio-demographic aspects and assertiveness     

Vesna Jovanović  Jasmina Karić  Goran Mihajlović  Tamara Džamonja-Ignjatović 《欧洲特需教育杂志》2013,28(5):692-701

ABSTRACT

The aim of this research was to examine the levels of burnout syndrome dimensions in special education teachers and correlations with some socio-demographic characteristics, job characteristics, and levels of assertiveness. The research included 225 special education teachers from Serbia (82% were women, 18% were men, with the average age of 42.51 ± 9.23 years). Research instruments included Maslach Burnout Inventory, Rathus Assertiveness Schedule, and a socio-demographic questionnaire. There were differences in all burnout dimensions in relation to types of students’ special needs. Higher levels of burnout symptoms were observed in teachers working with children with motor skill disorders. The assertiveness scores had a significant negative correlation with emotional exhaustion and depersonalisation, and a positive correlation with a lack of accomplishment. The results obtained may help in the planning of the adequate preventative measures for improving the mental health of those professionals.  相似文献   

How students perceive teachers’ nonverbal and verbal communication at agricultural universities in Austria,Slovenia and Albania     

Marija Klopčič  Renata Bažok  Helena Virić Gašparić  Karmen Erjavec 《The Journal of Agricultural Education and Extension》2018,24(5):473-483

Purpose: This study attempts to close the research gap created by the fact that existing studies neglect the problem of how effectively agricultural professors from different European countries communicate. The aim is to identify similarities and differences in the numbers of agricultural professors perceived by students as engaging in verbal and nonverbal immediacy communication.

Methodology: An online survey was conducted among students of agricultural universities from Austria, Slovenia and Albania.

Findings: The results show that professors of agriculture from Austria, Slovenia and Albania should generally not be satisfied with their own communication patterns and should thus try to improve their communication. The result also reveals cultural differences in the shares of agriculture professors employing different communication patterns in Austria, Slovenia and Albania. Compared to Austrian and Slovenian students, their Albanian peers perceive that most of their professors use nonverbal immediacy communication. According to Austrian students, the majority of their professors use verbal immediacy. On the contrary, Albanian students assessed that some of their professors employ verbal immediacy.

Practical Implications: The results show the professors of agriculture should improve the way they communicate to students. In particular, the Albanian professors should improve their verbal communication especially in terms of providing timely and quality feedback to students.

Theoretical implications: The study reveals differences in immediacy communication among countries (Austria, Slovenia and Albania) which the scientific literature considers to have a high-context culture.

Originality/Value: Given that no study has yet examined how students perceive professors’ communication in different European countries, this research helps understand the characteristics of agricultural professors’ communication.  相似文献   

Grading students' programming and soft skills with open badges: A case study     

Bojan Tomić  Jelena Jovanović  Nikola Milikić  Vladan Devedžić  Sonja Dimitrijević  Dragan Đurić  Zoran Ševarac 《British journal of educational technology : journal of the Council for Educational Technology》2019,50(2):518-530

Well-developed programming (technical) skills are very important for software engineers, information systems engineers and programmers in general. However, they must also possess relevant personal skills (soft skills) to be successful at the workplace (eg, collaboration, solving real-world problems and communication). The latter, however, are rarely assessed and acknowledged in regular software engineering courses. This paper describes the results of a small case study involving an extracurricular Java programming course in which, in addition to knowledge and skills in relevant technologies, students' soft skills were also assessed. As part of the assessment, students have been awarded Open Badges. The study was exploratory in nature, aimed at examining Open Badges as a motivational mechanism, students' engagement in attaining soft skills and students' perception of soft skills and Open Badges. The results suggest that Open Badges may not be so effective in motivating students to complete the assignments nor attend the course, although students' perception of Open Badges is generally positive. Soft skills were generally perceived as important as hard skills. Students' engagement in attaining soft skills could be affected by assignment announcement time and its level of difficulty.  相似文献   

Grades degrade group coordination: deteriorated interactions and performance in a cooperative motor task     

Anne-Sophie Hayek  Claudia Toma  Sofia Guidotti  Dominique Oberlé  Fabrizio Butera 《European Journal of Psychology of Education - EJPE》2017,32(1):97-112

At school, pupils often cooperate on common projects and must coordinate their different individual actions. However, grades are pervasively used even in cooperative situations, which make the pupils’ differences in achievement and their relative rank salient and may reduce their inclination to work constructively with others. Thus, we hypothesized that grades would elicit disruptive interactions and reduce performance in a cooperative cognitive-motor task necessitating inter-individual coordination of members. In a study with 5th graders, grades (vs. a neutral concept) were primed at the onset of a cooperative group interaction. Results showed that, although pupils were set to work cooperatively, priming grades (vs. neutral concepts) harmed inter-individual coordination by eliciting more negative dominant behaviours among pupils during interactions, which decreased group performance.  相似文献   

The organization of educational research in the Czechoslovak Socialist Republic     

Emilie Langová  Anna Střížová 《International Review of Education/Internationale Zeitschrift für Erziehungswissenschaft/Revue internationale l'éducation》1962,8(1):78-81

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A comparative study of wall painting materials and techniques used in fifteenth century Austria by Frederich of Villach and his followers     

Anabelle Križnar 《文物保护研究》2014,59(5):289-299

One of the most important artistic circles in the first half of the fifteenth-century in Austria was the so-called ‘Older Villach's workshop’, founded by Frederic of Villach, a painter who was considered a master of fresco technique. A technical study was made of a number of wall painting cycles by the workshop of Frederic of Villach, first, to gain a broader knowledge of the painting techniques employed, and second, to allow comparison with a further group of wall paintings in Slovenia, which are stylistically related to this workshop and have been studied previously. Of special interest were artworks attributed to Frederic's son Johannes of Ljubljana and a number of other anonymous painters that show important similarities to Frederic's works. Samples of plasters and pigments were analysed by optical microscopy, scanning electron microscopy with energy dispersive X-ray spectroscopy, Fourier transform infrared spectroscopy, and X-ray diffraction. An important difference in the composition of plasters was discovered between the selected artistic groups. The palette used comprised predominantly earth and other mineral pigments. The construction of the paintings from incisions and preparatory drawings to the final modelling is basically similar and shows workshop connections. The principal painting technique was a fresco. The results contribute to a wider knowledge concerning the materials and techniques employed in gothic wall paintings in the Alpine region and offer new information that can be used to inform the future conservation of these selected wall paintings.  相似文献   

Evaluation of mobile assessment in a learning management system     

Zorica Bogdanović  Dušan Barać  Branislav Jovanić  Snežana Popović  Božidar Radenković 《British journal of educational technology : journal of the Council for Educational Technology》2014,45(2):231-244

This article discusses the problem of using and delivering educational content from the Moodle learning management system to mobile devices. The primary goal of this study was to investigate the students' habits, motivations and technical possibilities in order to incorporate mobile‐learning activities in the e‐learning process. A mobile quiz has been chosen as an activity to be delivered on mobile devices. The developed mobile quiz application was integrated into Moodle. The application interface was tailored to various types of mobile devices aiming to foster mainstreaming of mobile technologies in e‐learning. For the purposes of the evaluation, an experiment was conducted within the e‐learning system at the Faculty of Organizational Sciences, the University of Belgrade. Data were obtained from the students who attended e‐learning courses in the area of e‐business. Research findings have shown that integration of the mobile quiz application into Moodle improves students' results and increases satisfaction and motivation for using mobile devices in their learning process.  相似文献   

Non-invasive methods for characterisation of printed cultural heritage     

Klementina Možina  Klemen Možina  Sabina Bračko 《Journal of Cultural Heritage》2013,14(1):8-15

The quality of a book reprint depends on several factors, e.g. paper, typesetting, reproduction of illustrations, printing and bookbinding. The quality of the 1958 and 1988 reprints of the fairytale edition from 1944 designed by the Slovenian architect Jo?e Ple?nik was studied using standard and other non-invasive testing methods, e.g. microscopic and spectroscopic techniques, sonic velocity, Young's modulus of elasticity, surface topography and image analysis. The chemical, physical and colorimetric properties of papers and typographic tonal density were analysed. The results showed that the reprints do not correspond to the originals. Some of the differences in the reprints, if compared with the original (e.g. typographic tonal density), could have been easily avoided, while others (e.g. structural and optical properties of paper) remain unavoidable, mainly due to the influence of internal and external factors on the ageing. The ageing process influences paper properties, since optical properties deteriorate in time. It has been concluded that a precise and systematic study of the properties of an old book should be performed before the preparation of a reprint. The results of the research have shown that the applied methods are useful and satisfactory for the characterisation of the paper properties and typography, and can be of use at the analysis of printing ink and illustration reproduction.  相似文献