排序方式： 共有21条查询结果，搜索用时 328 毫秒
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利用ISSR技术检验辣椒品种纯度实验初探 总被引：1，自引：0，他引：1
作物品种鉴定对农业生产影响显著,而常规品种纯度鉴定往往过程繁琐、周期过长、主观性强,已不能满足日益发展的生产需要．新兴起的分子标记技术无表型效应,结果更为客观准确．文章通过ISSR分子标记技术对6份辣椒品种进行了纯度鉴定的初步实验探索,结果表明利用该技术完全可以进行纯度鉴定,而且周期短、结论明确,不失为今后的一种发展方向． 相似文献
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红树植物海桑简单重复序列区间(ISSR)条件的优化 总被引：17，自引：0，他引：17
海桑(Sonneratiacaseolaris(L.)Engler)是海桑科一种重要的红树植物.为利用ISSR分子标记研究海桑的遗传多样性,对影响ISSR PCR的多个因素,包括Taq酶的用量、Mg2+浓度和dNTP浓度、引物浓度及其退火温度、模板DNA浓度等进行研究,确定了海桑ISSR PCR分析的最适条件:10μLPCR反应体积中,10×buffer(100mmol·L-1KCl,80mmol·L-1(NH4)2SO4,100mmol·L-1Tris HCl,pH9.0,0.5%NP 40)1μL,0.35UTaq酶,2～3.75mmol·L-1Mg2+,300μmol·L-1dNTP,0.3μmol·L-1引物,10ng模板DNA. 相似文献
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韩颖 《内江师范学院学报》2008,23(2):51-54
采用改进的CTAB法,成功地提取了北野豌豆及其近缘种的基因组DNA,电泳结果显示DNA质量和产量均较高,并以此DNA为模板,对ISSR的反应条件进行了优化,最终确定ISSR的反应体系为：总体积10μl,包括10ng的DNA,1倍的反应缓冲液,1．5mmol·L^-1MgCl2,2×10^-4mmol·L^-1（引物6和引物8）或2×10^-3mmol·L^-1（引物5,10和12）引物,0．2mmol·L^-1dNTP混合物和1．0U的Taq DNA聚合酶．ISSR扩增程序为：先在94℃下变性3min,然后在92℃下变性30s,50℃（引物5,6,10,12）或55．2℃（引物8）下复性30s,72℃下延伸1min,反应30个循环,最后72℃延伸5min．并基于此反应条件,最终筛选出5条ISSR引物用于进一步的研究． 相似文献
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不同花色紫薇的ISSR分析 总被引：1，自引：0，他引：1
以ISSR标记技术,对不同花色的4个紫薇群体(红色紫薇群体、白色紫薇群体、紫色紫薇群体及粉色紫薇群体)共48个样品进行分析,筛选得到4个较好的特异性引物,共扩增出30个清晰的扩增位点,其中多态性位点23个,多态位点百分率为76.67%.利用POPGENE软件分析得到,四个紫薇群体间的Shannon's信息指数为0.4186,基因多样性指数(Ht)为0.2806,表明这四个紫薇群体具有较丰富的遗传多样性.聚类分析结果表明白色、紫色及粉色系的单株基本聚在一起,但红色系的聚类结果比较分散,且红色和白色亲缘关系较近,与粉色的亲缘关系较远. 相似文献
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BackgroundSargassum liebmannii is widely distributed throughout rocky, coastal upwelling areas in the tropical Mexican Pacific. This brown algae is of great environmental and industrial importance. However, no information is available that documents the genetic or phenotypic variability of the species, which is needed to determine how it may react to environmental variation related to climate change. In this study, S. liebmannii specimens were collected from the coast of Jalisco, Mexico, and molecular and morphological characterization was conducted. Intraspecific variability was estimated according to the study areas.ResultsThe inter-simple sequence repeat (ISSR) markers indicated a polymorphism percentage of 95%. The Shannon index and Nei index showed relatively low values among the populations (0.3569 and 0.081, respectively). On the other hand, the genetic differentiation coefficient indicated inter- and intrapopulation values of 36.69% and 63.31%, respectively. The Jaccard similarity coefficient was used to determine the degree of similarity among individuals by geographical area. The morphological characteristics and environmental variables that were used to correlate phenotypes and genotypes indicated that S. liebmannii showed low genetic flow because of the presence of geographical barriers due to substrate that was not optimal for algal development.ConclusionsThe ISSR markers were useful for detecting genetic differences among S. liebmannii individuals. The results indicate that a coupled genotypic-phenotypic study is beneficial for documenting the variation present in the little-studied algal species. These studies may be used in future research to clarify taxonomic controversies while generating additional genomic information.How to cite: Jung-Kim HW, Hernández-Herrera RM, Enciso-Padilla I, et al. Genetic variability of Sargassum liebmannii on the coast of Jalisco in the central Mexican Pacific revealed by molecular markers and morphological traits. Electron J Biotechnol 2021;54. https://doi.org/10.1016/j.ejbt.2021.08.003 相似文献
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