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Wei-dong Yang Yu-yan Wang Feng-liang Zhao Zhe-li Ding Xin-cheng Zhang Zhi-qiang Zhu Xiao-e Yang 《Journal of Zhejiang University. Science. B》2014,15(9):788-800
Willows (Salix spp.) have shown high potential for the phytoextraction of heavy metals. This study compares variations in copper (Cu) and zinc (Zn) tolerance and accumulation potential among 12 willow clones grown in a nutrient solution treated with 50 μmol/L of Cu or Zn, respectively. The results showed differences in the tolerance and accumulation of Cu and Zn with respect to different species/clones. The biomass variation among clones in response to Cu or Zn exposure ranged from the stimulation of growth to inhibition, and all of the clones tested showed higher tolerance to Cu than to Zn. The clones exhibited less variation in Cu accumulation but larger variation in Zn accumulation. Based on translocation factors, it was found that most of the Cu was retained in the roots and that Zn was more mobile than Cu for all clones. It is concluded that most willow clones are good accumulators of Zn and Cu. 相似文献
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刘秀花 《商丘师范学院学报》2007,23(9):99-103,114
基因组学的出现极大地影响了微生物学的基础研究和应用研究．枯草芽孢杆菌（Bacillussubtilis）是一个用于基础研究和生物工艺研究的重要模式细菌．从它的基因组获得的知识远比长期以来用传统的方法获得的信息要多．通过先进的方法分析其基因组和转录组，基本弄清了关于枯草芽孢杆菌染色体结构的信息，已经达到了能够阐明其基因表达过程的水平，使得完全掌握枯草芽孢杆菌分泌途径的生物化学操作成为可能，这将成为生物工艺学的重大成果、在理论上，随着对它的分析越来越细致，已经阐明一个遗传学的海量信息的新观念，直至获得理解再造一个细胞所需的最小的基因组的知识．总之，枯草芽孢杆菌基因组知识已经明显地更新了基础知识向应用微生物学和生物工艺学转化的途径，并使这种细菌成为后基因组微生物学的重要工具． 相似文献
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在不同的生长调节剂组合的培养基上 ,取绿巨人的诱导芽进行继代培养 ,以产生大量的丛生芽 ,同时探讨了不同生长激素和浓度对丛生芽和不定根分化的影响 .结果表明MS +BA 0 5～ 1 +IBA/NAA 0 1 (mg/L)有较高的繁殖系数和成苗率以及成本低等优点 ;而BA 1～ 2 +NAA 0 2～ 0 5有利丛生芽分化 ,繁殖系数高 . 相似文献
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采用正交实验法分析出绿色木霉 3.3711纤素酶系各组分最适酶解条件组合分别为(pH5 .0、4 5℃ ) (C1酶 ) ,(pH3.5、5 5℃ ) (Cx 酶 )和 (pH4 .5、6 5℃ ) (βG酶 )。分析比较了分别使用纤维素粉和麦麸为单一或混合碳源时在产酶过程中各组分酶的最高酶活 ,比酶活及其形成时间和分泌特性。结果表明 ,同种碳源不同浓度所产酶系中C1比酶活相对稳定 ,C1和Cx 酶活分别形成一个和两个高峰 ,βG酶均为一个高峰。混合碳源使各组分酶活高峰期相对一致 ,高峰期稳定时间延长和酶量增加。 相似文献
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Enhancing rice resistance to fungal pathogens by transformation with cell wall degrading enzyme genes from Trichoderma atroviride 总被引：1，自引：0，他引：1
INTRODUCTION Rice sheath blight (Rhizoctonia solani) and rice blast (Magnaporthe grisea) are two serious plantdiseases in China. Rice cultivars with high level and durable resistance to these two pathogens are noteasy to obtain by traditional breeding methods due to the lack of germplasm resistant to R. solani and the high genetic diversity of M. grisea. Cell wall degrading enzyme (CWDE) genes from biocontrol fungi belonging to the genus Trichoderma have been demonstrated to encode … 相似文献
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利用荧光假单胞杆菌(Pseudomonas fluorescens)WCS374r、WCS417r、Q2-87、2-79和CHAOr,恶臭假单胞杆菌(P.putida)WCS358r菌株及其转基因菌株WCS358::phl和WCS358::Phz,以及绿脓假单胞杆菌(P.aeruginosa)7NSK2及其相应的嗜铁素突变体,采用预先接种和混合接种两种方法开展了板粟疫病的生物防治试验.在对板栗疫病菌的拮抗活性测定中,所用生防菌对其孢子萌发和菌丝的生长有不同程度的抑制作用,其中以能产生嗜铁素的WCS358r等菌株,优于其嗜铁素缺失突变体,说明嗜铁素是抑制板栗疫病菌孢子萌发的有效因子.在生物防治试验中,WCS358r对板栗疫病有较好的防治效果,其嗜铁素缺失突变体JM218却没有效果,其转基因菌株WCS358::phl的发病率与WCS358r接近,说明细菌产生的嗜铁素有一定的防治作用,而细菌产生的抗生素DAPG没有防治效果.混合接种比预先接种防治效果提高了10%左右. 相似文献
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Kubicek CP Komon-Zelazowska M Druzhinina IS 《Journal of Zhejiang University. Science. B》2008,9(10):753-763
Hypocrea/Trichoderma is a genus of soil-borne or wood-decaying fungi containing members important to mankind as producers of industrial enzymes and biocontrol agents against plant pathogens, but also as opportunistic pathogens of immuno-compromised humans and animals, while others can cause damage to cultivated mushroom. With the recent advent of a reliable, BarCode-aided identification system for all known taxa of Trichoderma and Hypocrea, it became now possible to study some of the biological fundamentals of the diversity in this fungal genus in more detail. In this article, we will therefore review recent progress in (1) the understanding of the geographic distribution of individual taxa; (2) mechanisms of speciation leading to development of mushroom diseases and facultative human mycoses; and (3) the possible correlation of specific traits of secondary metabolism and molecular phylogeny. 相似文献
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对毛乌素沙地荒漠草原围封样地狭叶锦鸡儿 小针茅群落地上生物量进行了 7年逐月连续观测,建立了生物量对气候响应的逐月回归模型。研究表明,光、温、水等不同的气候因子在植物生长不同时期表现出不同的影响,其中降水因子在生长季各个月份都是显著影响因子,说明水分的限制作用突出。应用逐月回归模型对三种不同气候条件下生物量的波动范围进行预测,并将逐月回归模型与传统的累积回归模型的模拟结果进行比较,表明在大多数年份逐月回归模型具有更高的精度。逐月回归模型是对传统的累积回归建模方法的有益改进。 相似文献