排序方式： 共有29条查询结果，搜索用时 187 毫秒
1.
耐受重金属微生物资源的筛选与分子鉴定 总被引：2，自引：1，他引：2
从77种芽孢杆菌中筛选出的杀虫细菌苏云金杆菌4AFl，其耐受重金属的最低抑制浓度（minimum inhibitory concentration，MIC）分别为：Cu^2＋124．0mM、Co^2＋277．2mM、Zn^2＋51．1mM、Ni^＋109．4mM．复合污染下，Co^2＋对4AF1菌的毒性被激活，Ni^＋的毒性受抑制．从混合土壤样品及泉州洛江水样中筛选到5个耐受细菌和3个耐受真菌．16S rDNA高变区序列的PCR扩增与测定结果表明．耐受菌1为气单胞杆菌属细菌．耐受菌3为假单胞杆菌属细菌． 相似文献
2.
The yeast strain （Y18） was isolated from a soil sample collected from Fildes Peninsula, Antarctica. The strain is a psychrophilic yeast with optimum and maximum growth temperatures of 10 ℃ and 18 ℃, respectively. Teliospores were formed after 7 d on malt agar, when the germination of teliospores was observed. Both inositol and D-glucuronate were assimilated. Positive results of the DBB （diazonium blue B） color reaction, urease test, and starch formation were observed. The major CoQ is Qs. All results indicated that Y18 belongs to the genes of Mrakia. The 18S rDNA sequence analyses showed that Y18 is closely related to Mrakiafrigida. DNA-DNA relatedness study, and some biochemistry characteristics indicated that Y18 represents a new species for which Mrakia psychrophila sp. nov. is proposed. 相似文献
3.
为了对黄斑姜(Zingiber flavo-maculatum S.Q.Tong.)的染色体进行识别并对该物种基因组的结构进行初步研究,利用PI和DAPI组合(CPD)染色和45S rDNA探针荧光原位杂交对中期染色体进行了分析.结果显示,黄斑姜具有2对45S rDNA位点,分别位于第3、4号染色体的短臂,对应于相应染... 相似文献
4.
在反应器挂膜启动阶段和运行阶段投加好氧反硝化菌纯培养物,进行生物强化反硝化研究。结果表明,在反应器启动过程中投加好氧反硝化菌,可缩短系统的启动时间;在反应器运行过程中间歇投加好氧反硝化菌能提高系统对污染物的去除效果,同时可增强系统处理的稳定性。 相似文献
5.
浙江沿海一新纽虫的18S rDNA序列研究 总被引：1，自引：0，他引：1
为进一步确定浙江沿海发现的一新纽虫的分类地位,本文根据GenBank中已报道的纽虫18SrDNA保守序列设计引物,通过PCR扩增,获得该纽虫的18SrDNA部分序列,对该纽虫的18SrRNA基因进行了克隆和序列分析。并将该纽虫18SrDNA基因的序列与NCBI数据库中已收纽虫的18SrDNA基因进行了比较,分析该纽虫与其他纽虫的同源性。利用系统进化树和形态学的研究结果确定其分类地位。结果表明,该纽虫属于异纽目（Heteronemertea）纵沟虫科（Lineidae）动物。 相似文献
6.
Wang AR Lin WW Chen XT Lu GD Zhou J Wang ZH 《Journal of Zhejiang University. Science. B》2008,9(10):818-822
A new stem rot disease is found to occur naturally on Arabidopsis plants in greenhouses of Fuzhou, China. In order to identify its pathogen, we conducted a series of fimgal isolation and purification, plant reinoculation, and ascus and ascospore induction from the sclerotia. The isolate caused typical water-soaked lesions after reinoeulation and produced sclerotia both on Arabidopsis plants and culture medium plates, and the sclerotia could be induced to produce discal apothecia and 8 binucleate ascospores per ascus. These disease symptom and fungal morphology data revealed that the fungus Sclerotinia sclerotiorum (Lib.) de Bary was the pathogen for Arabidopsis stem rot. To confirm this, we further amplified its large subunit ribosomal DNA (LSU rDNA) by polymerase chain reaction (PCR), and compared the sequence with the known LSU rDNA sequences in GenBank. The results show that the sequence shares the highest identities with the LSU rDNAs of different S. sclerotiorum strains. Taking all these data together, we concluded that the fungus that caused the Arabidopsis stem rot is S. sclerotiorum (Lib.) de Bary. This is the first report that Arabidopsis is naturally infected by S. sclerotiorum. 相似文献
7.
采用18S rDNA序列为基础的系统分析方法,对采自嘉陵江磁器口江段的黄颡单尾虫Unicauda pelteobagrus Ma,1998进行了分子系统学研究,首次给出了黄颡单尾虫的SSU rDNA序列信息．同时基于18S rDNA数据、遗传距离和系统树特征讨论了尾单尾虫Unicauda caudatus和黄颡单尾虫的系统关系．结果表明,尾单尾虫与尾碘泡虫的系统关系较近,而与黄颡单尾虫系统关系较远,不支持将尾单尾孢虫和黄颡单尾虫同置于单尾虫属中,并对单尾虫属的有效性提出质疑． 相似文献
8.
Chen-jian Liu Fu-ming Gong Xiao-ran Li Hai-yan Li Zhong-hua Zhang Yue Feng Hiroko Nagano 《Journal of Zhejiang University. Science. B》2012,13(4):298-306
This research was aimed at isolating and identifying the predominant lactic acid bacteria (LAB) in the traditional Chinese
salt-fermented soybean food, douchi, from Yunnan, China. The predominant LAB present were isolated and identified by conventional
culture-dependent methods combined with molecular biological methods. Two hundred and sixty isolates were obtained from thirty
kinds of traditional fermented douchi from six cities and counties in Yunnan, and those strains were divided into twelve groups
by their morphological and biochemical characteristics. Based on 16S ribosomal DNA (rDNA) sequencing and analysis, 56 representative
strains were identified as belonging to 6 genera and 14 species: Lactobacillus (4 spp.), Weissella (3 spp.), Pediococcus (2 spp.), Staphylococcus (2 spp.), Enterococcus (1 sp.), and Bacillus (2 spp.). The results show that douchi contains a large natural population of LAB of diverse composition from which some
strains could be selected as starters for functional fermented foods. This is the first study on the original douchi from
Yunnan, and the results suggest that it may be a useful source for the isolation of LAB. This study has also laid a foundation
for further research on developing functional douchi products. 相似文献
9.
Ines Ben Chobba Amine Elleuch Imen Ayadi Lamia Khannous Ahmed Namsi Frederique Cerqueira Noureddine Drira Néji Gharsallah Tatiana Vallaeys 《Journal of Zhejiang University. Science. B》2013,14(12):1084-1099
Endophytic flora plays a vital role in the colonization and survival of host plants, especially in harsh environments, such as arid regions. This flora may, however, contain pathogenic species responsible for various troublesome host diseases. The present study is aimed at investigating the diversity of both cultivable and non-cultivable endophytic fungal floras in the internal tissues (roots and leaves) of Tunisian date palm trees (Phoenix dactylifera). Accordingly, 13 isolates from both root and leaf samples, exhibiting distinct colony morphology, were selected from potato dextrose agar (PDA) medium and identified by a sequence match search wherein their 18S–28S internal transcribed spacer (ITS) sequences were compared to those available in public databases. These findings revealed that the cultivable root and leaf isolates fell into two groups, namely Nectriaceae and Pleosporaceae. Additionally, total DNA from palm roots and leaves was further extracted and ITS fragments were amplified. Restriction fragment length polymorphism (RFLP) analysis of the ITS from 200 fungal clones (leaves: 100; roots: 100) using HaeIII restriction enzyme revealed 13 distinct patterns that were further sequenced and led to the identification of Alternaria, Cladosporium, Davidiella (Cladosporium teleomorph), Pythium, Curvularia, and uncharacterized fungal endophytes. Both approaches confirmed that while the roots were predominantly colonized by Fusaria (members of the Nectriaceae family), the leaves were essentially colonized by Alternaria (members of the Pleosporaceae family). Overall, the findings of the present study constitute, to the authors’ knowledge, the first extensive report on the diversity of endophytic fungal flora associated with date palm trees (P. dactylifera). 相似文献
10.
为探讨rDNA在重楼属Paris L.中的分布规律，利用荧光原位杂交（FISH）对4种重楼属植物
的18-26S rDNA进行了定位。所有植物均为二倍体，基因组由A、B、C、D和
E5条染色体构成。（1）滇重楼P.polyphylla var.yunnanensis:2n=10=6m+4t，C和D染色体的
短臂上各有1个18-26S rDNA位点;（2）长柱重楼P.forrestii:2n=10=6m+4t，B染色体的长臂
、C和D染色体的短臂上各有1个位点;（3）五指莲P.axialis:2n=10=6m（2sat）+4t（2sat）
+1-2B，C和D染色体的短臂上各有1个位点;在有1个B染色体的细胞中，B染色体没有信号点，
而有2个B染色体的细胞中，只有1个B染色体上有信号点，表明B染色体上有基因存在且其分裂
不均等;（4）大理重楼P.daliensis:2n=10=4m+2sm+2st+2t，C染色体的短臂上有1个位点。1
8-26S rDNA位点不仅出现在染色体的次缢痕上，也出现在非次缢痕位点。另外，4个种中C染
色体短臂末端均有18-26S rDNA。 相似文献