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弓形虫感染对优生优育的危害 总被引：2，自引：0，他引：2
弓形虫是一种分布广危害大的常见寄生虫。孕妇感染弓形虫后，虫体可垂直传递给胎儿，导致胎儿产生各种严重的先天性疾病。因此，弓形虫感染对优生优育的危害是十分严重的。 相似文献
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为构建弓形虫主要表面抗原1(SAG1)的原核重组表达质粒并获得重组表达蛋白,采用聚合酶链反应(PCR)从弓形虫RH株基因组DNA中扩增SAG1基因,经克隆和测序后,亚克隆至原核表达载体pGEX-4T-1,转化大肠杆菌BL21,PCR和双酶切鉴定阳性菌株,表达产物用SDS-PAGE和Western blot进行鉴定观察其诱导的抗体应答.结果表明,PCR扩增出SAG1基因的特异片段,获得的阳性克隆序列与GenBank中的SAG1基因序列一致,SAG1基因被亚克隆到表达载体pGEX-4T-1,在BL21中表达了SAG1,表达的蛋白能被弓形虫感染兔血清识别.以上结果说明,已成功构建了弓形虫SAG1的重组表达质粒,在大肠杆菌中表达的SAG1具有良好的免疫学活性. 相似文献
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Rui CHEN Shao-hong LU Qun-bo TONG Di LOU Dong-yan SHI Bing-bing JIA Guo-ping HUANG Jin-fu WANG 《Journal of Zhejiang University. Science. B》2009,10(7)
The dense granule protein 4 (GRA4) is a granular protein from Toxoplasma gondii, and is a candidate for vaccination against this parasite. In this study, the plasmid pcDNA3. 1-GRA4 (pGRA4), encoding for the GRA4 antigen, was incorporated by the dehydration-rehydration method into liposomes composed of 16 mmol/L egg phosphatidylcholine (PC), 8 mmol/L dioleoyl phosphatidylethanolamine (DOPE), and 4 mmol/L 1,2-diodeoyl-3-(trimethylammonium) propane (DOTAP). C57BL/6 mice and BALB/c mice were immunized intramuscularly three times with liposome-encapsulated pGRA4 to determine whether DNA immunization could elicit a protective immune response to T. gondii. Enzyme-linked immunosorbent assay (ELISA) of sera from immunized mice showed that liposome-encapsulated pGRA4 generated high levels of IgG antibodies to GRA4. Production of primary interferon (IFN)-γ and interleukin (IL)-2 in GRA4-stimulated splenocytes from vaccinated mice suggested a modulated Th1-type response. 72.7% of C57BL/6 mice immunized with liposome-encapsulated pGRA4 survived the challenge with 80 tissue cysts of ME49 strain, whereas C57BL/6 mice immunized with pGRA4 had only a survival rate of 54.5%. When immunized BALB/c mice were intraperitoneally challenged with 103 tachyzoites of the highly virulent RH strain, the survival time of mice immunized with liposome-encapsulated pGRA4 was markedly longer than that of other groups. Our observations show that liposome-encapsulated pGRA4 enhanced the protective effect against infection of T. gondii. 相似文献
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为构建弓形虫主要表面抗原1(SAG1)的原核重组表达质粒并获得重组表达蛋白,采用聚合酶链反应(PCR) 从弓形虫RH 株基因组DNA中扩增SAG1基因,经克隆和测序后,亚克隆至原核表达载体pGEX-4T-1,转化大肠杆菌BL21,PCR和双酶切鉴定阳性菌株,表达产物用SDS-PAGE和Western blot进行鉴定观察其诱导的抗体应答.结果表明,PCR扩增出SAG1基因的特异片段,获得的阳性克隆序列与Gen Bank中的SAG1基因序列一致,SAG1基因被亚克隆到表达载体pGEX-4T-1,在BL21中表达了SAG1,表达的蛋白能被弓形虫感染兔血清识别.以上结果说明,已成功构建了弓形虫SAG1的重组表达质粒,在大肠杆菌中表达的SAG1具有良好的免疫学活性. 相似文献
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山西省大同市男性不育患者弓形虫感染情况调查 总被引：1，自引：0，他引：1
焦雨 《河北北方学院学报(医学版)》2006,23(1):53-54
目的：探讨男性不育患者弓形虫（TOX）感染的状况及感染后对男性生殖功能的影响。方法：用酶联免疫吸附试验（ELISA）法检测男性不育患者血中弓形虫循环抗原（CAg），IgG及IgM抗体。结果：100例男性不育患者血清中CAg阳性者13例（13％），TOX—IgM阳性者16例（16％）；而100例生殖功能正常的男性血清中CAg阳性者仅为1例（1％），TOX—IgM阳性者3例（3％）；两者间差异非常显著（P〈0．01）。两者TOX-IgG阳性者均为7例（7％）。结论：TOX急性（早期）感染可能会影响男性生育能力，并导致男性不育。因此，男性也应注意避免感染弓形虫，防止导致不育的情况发生。 相似文献
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