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1.
目的:探究镧对大豆铝胁迫的缓解效应;方法:以铝敏感大豆BD2为材料,进行50μmol/L铝胁迫处理,然后添加不同浓度氯化镧(10、100和1000 mg L/L)测定大豆幼苗抗氧化及光合指标;结果:与对照组相比较,铝胁迫导致大豆幼苗根系伸长率、根冠比、鲜重及干重显著降低,叶片SOD、POD活性显著降低,且MDA含量显著升高,叶绿素与类胡萝卜素含量、光合速率、蒸腾速率、气孔导度及胞间CO_2浓度也均显著下降;而与铝胁迫处理相比,不同浓度镧处理对大豆幼苗根长、鲜重与干重、SOD与POD活性有不同程度地促进作用,并显著降低MDA含量,同时提高叶绿素与类胡萝卜素含量,增强光合速率、蒸腾速率、气孔导度和胞间CO_2浓度,其中以10 mg/L镧处理效果最好;结论:适宜浓度的镧处理能够通过增强大豆幼苗的抗氧化能力,提高其光合性能,提高大豆幼苗对铝胁迫的适应能力,有效缓解铝对大豆幼苗生长的毒害。 相似文献
2.
BackgroundDiabetes is a metabolic disorder caused by defects in insulin production and activity. During disease progression, changes in lipid peroxidation cause structural modifications via production of free radicals. Fangchinoline is a well-known alkaloid present in Stephaniae tetrandrine S. Moore, which has demonstrated antioxidant, anticancer, and anti-inflammatory activities.ResultsThe present study analyzed the anti-diabetic and antioxidant effects of fangchinoline in male rats with streptozotocin-induced diabetes. Rats were divided into the following groups: normal control, diabetic, diabetic + fangchinoline 100 mg/kg, diabetic + fangchinoline 200 mg/kg and diabetic + glibenclamide 600 µg/kg. The treatment was administered orally for 45 consecutive days. Lipid peroxidation was substantially increased by >50% in the serum, as well as the liver, kidney, and heart tissues of diabetic rats. However, fangchinoline supplementation significantly reduced lipid peroxidation to near normal levels. Reactive oxygen species levels were substantially increased by >500% in the serum, as well as the liver, kidney, and heart tissues of diabetic rats. Fangchinoline supplementation reduced reactive oxygen species to near normal levels. Fangchinoline supplementation significantly improved superoxide dismutase, glutathione peroxidase, catalase, and reduced glutathione levels in diabetic rats. Total hexoses, sialic acid, hexosamines, and fucose were increased in diabetic rats, whereas fangchinoline supplementation significantly reduced these total hexoses, sialic acid, hexosamines, and fucose to near normal levelsConclusionsSupplementation with fangchinoline led to significant attenuation of the levels of lipid peroxidation, ROS, and glycoprotein components such as total hexoses, hexosamines, sialic acid, and fucose, while improving antioxidant marker levels.How to cite: Xia J, Huang W, Zhou F. Effect of fangchinoline on oxidant status in male albino rats with streptozotocin-induced diabetes. Electron J Biotechnol 2021;53. https://doi.org/10.1016/j.ejbt.2021.07.005 相似文献
3.
Leaf senescence is often caused by water deficit and the chimeric gene PSA612-1PT is an auto-regulated gene delaying leaf senescence. Using in vitro leaf discs culture system, the changes of contents of chlorophylls, carotenoids, soluble protein and thiobarbituric acid reactive substance （TBARS） and antioxidant enzymes activities were investigated during leaf senescence of PSA612-1PT modified gerbera induced by osmotic stress compared with the control plant （wild type）. Leaf discs were incubated in 20%, 40% （w/v） polyethylene glycol （PEG） 6 000 nutrient solution for 20 h under continuous light [ 130 μmol/（m^2·s）]. The results showed that the contents of chlorophylls, carotenoids and soluble protein were decreased by osmotic stress with the decrease being more pronounced at 40% PEG, but that, at the same PEG concentration the decrease in the transgenic plants was significantly lower than that in the control plant. The activities of superoxide dismutase （SOD）, catalases （CAT）, ascorbate peroxidase （APX）, guaiacol peroxidase （GPX） and dehydroascorbate reductase （DHAR） were stimulated by PEG treatment. However, the increases were higher in PSA612-IPT transgenic plants than in the control plants, particularly at 40% PEG treatment. Lipid peroxidation （TBARS content） was increased by PEG treatment with the increase being much lower in transgenic plant than in the control plant. It could be concluded that the increases in the activities ofantioxidant enzymes including SOD, CAT, APX, GPX and DHAR were responsible for the delay of leaf senescence induced by osmotic stress. 相似文献
4.
Three cooking methods, namely boiling, steaming, and stir-frying for 5 to 10 min, were used to evaluate the effect on nutrient
components, free amino acids, l-ascorbic acid, total phenolic contents, and antioxidant capacities of bamboo shoots (Phyllostachys praecox). Results showed that boiling and stir-frying had a great effect on the nutrient components and they decreased the contents
of protein, soluble sugar, and ash, and caused a great loss in the total free amino acids (decreased by 38.35% and 34.86%,
respectively). Significant differences (P<0.05) in free amino acids were observed in the samples cooked by different methods. Stir-fried bamboo shoots had a high fat
content which increased by 528.57% because of the addition of edible oil. After boiling, the L-ascorbic acid and total phenolic
contents were significantly reduced, while steaming increased total phenolic content by 3.98% and stir-frying well-preserved
L-ascorbic acid (78.87% of its previous content). Results of the antioxidative property study showed that stir-frying could
increase antioxidant capacities of bamboo shoots. It is concluded that stir-frying is more suitable for bamboo shoots because
it could obtain the maximum retention of antioxidant capacities. 相似文献
5.
Evaluation of the antioxidant effects of four main theaflavin derivatives through chemiluminescence and DNA damage analyses 总被引：1，自引：0，他引：1
Theaflavins (TFs) are the dimers of a couple of epimerized catechins, which are specially formed during black tea fermentation.
To explore the differences among four main TF derivatives (theaflavin (TF1), theaflavin-3-gallate (TF2A), theaflavin-3′-gallate (TF2B), and theaflavin-3,3′-digallate (TF3)) in scavenging reactive oxygen species (ROS) in vitro, their properties of inhibiting superoxide, singlet oxygen, hydrogen
peroxide, and the hydroxyl radical, and their effects on hydroxyl radical-induced DNA oxidative damage were systematically
analyzed in the present study. The results show that, compared with (−)-epigallocatechin gallate (EGCG), TF derivatives were
good antioxidants for scavenging ROS and preventing the hydroxyl radical-induced DNA damage in vitro. TF3 was the most positive in scavenging hydrogen peroxide and hydroxyl radical, and TF1 suppressed superoxide. Positive antioxidant capacities of TF2B on singlet oxygen, hydrogen peroxide, hydroxyl radical, and the hydroxyl radical-induced DNA damage in vitro were found.
The differences between the antioxidant capacities of four main TF derivatives in relation to their chemical structures were
also discussed. We suggest that these activity differences among TF derivatives would be beneficial to scavenge different
ROS with therapeutic potential. 相似文献
6.
A. K. Pradhan A. K. Shukla M. V. R. Reddy N. Garg 《Indian journal of clinical biochemistry : IJCB》2004,19(1):83-87
Oxidative stress was assesed by estimating lipid peroxidation product (LPO) in the form of thiobarbituric acid reactive substances
(TBARS), enzymatic antioxidants in the form of superoxide dismutase (SOD), catalase and nonenzymatic antioxidant vitamins
e.g. vitamin C, β carotene and vitamin E in either serum or plasma or erythrocytes in 190 cases of age related cataract in
the age group of 50–80 years. 190 cases were grouped into three morphological types namely, 73 cases of cortical, 77 cases
of posterior subcapsular and 40 cases of nuclear cataract and values of LPO and antioxidants were compared with 78 cases of
age matched healthy control groups. Plasma TBARS levels were cataract cases when compared with control groups. There were
no significant differences in the erythrocyte levels of catalase and plasma levels of Vit E between cataract cases and control
groups. No significant changes of parameters were seen among three different morphological types of age related cataract.
The present study shows that the oxidative stress may play an important role in the age related cataract. 相似文献
7.
D. Puri K. M. Prabhu G. Dev S. Agarwal P. S. Murthy 《Indian journal of clinical biochemistry : IJCB》2011,26(4):335-346
To study the mechanism of action of water soluble compound GII purified from fenugreek (Trigonella foenum graecum) seeds which was shown earlier to have antidiabetic effect in the subdiabetic, moderately and severely diabetic rabbits.
In rabbits (1–1.5 kg bw) diabetes was induced by intravenous injection of 80 mg/kg bw of alloxan. They were fed with GII at
a dose of 50 mg/kg bw daily once in the morning for 15 days in the subdiabetic and moderately diabetic and 30 days in the
severely diabetic rabbits. Serum total cholesterol (TC), triglycerides (TG), LDL + VLDL cholesterol [(LDL + VLDL)C], HDL cholesterol
[(HDL)C], total tissue lipids, glycogen and enzymes of carbohydrate metabolism (glycolysis, gluconeogenesis, polyol pathway)
hexokinase, glucokinase, pyruvate kinase, malic enzyme, glucose-6-phosphatase, glucose-6-phosphate dehydrogenase, aldose reductase
and sorbitol dehydrogenase and antioxidant enzymes glutathione peroxidase, glutathione reductase and superoxide dismutase
were estimated. Liver and kidney function parameters were also estimated. Treatment with GII for 15 days in the subdiabetic
and moderately diabetic rabbits and for 30 days in the severely diabetic rabbits (i) decreased the elevated lipids TC, TG,
(LDL + VLDL)C and increased the decreased (HDL)C, (ii) decreased the elevated liver and heart total lipids, TC and TG, (iii)
increased the decreased liver and muscle glycogen, (iv) increased the decreased hexokinase, glucokinase, pyruvate kinase,
malic enzyme, glucose-6-phosphate dehydrogenase, superoxide dismutase, glutathione peroxidase, (v) decreased the increased
glucose-6-phosphatase, sorbitol dehydrogenase, aldose reductase. Results thus show that treatment with GII compound purified
from fenugreek seeds for 15 days in the subdiabetic and moderately diabetic and 30 days in the severely diabetic rabbits corrects
the altered serum lipids, tissue lipids, glycogen, enzymes of glycolysis, gluconeogenesis, glycogen metabolism, polyol pathway
and antioxidant enzymes. Histopathological abnormalities (fatty infiltration and other cellular changes) seen in the pancreas,
liver, heart and kidneys were repaired after treatment with GII. In fact partially damaged pancreas was repaired. Liver and
kidney function test results were normal in the GII treated animals indicating that GII treatment is safe and free from any
side effects. 相似文献
8.
Yue-fei Wang Jie Wang Jing Wu Ping Xu Yi-qi Wang Jun-jie Gao Danielle Hochstetter 《Journal of Zhejiang University. Science. B》2014,15(2):173-180
The conditions for extracting polysaccharides from tea (Camellia sinensis L.) fruit peel (TFPPs) were studied. Three parameters (temperature, time, and liquid/solid ratio) affecting the extraction of TFPP were optimized using response surface methodology (RSM). Under the optimized conditions, the yield of TFPP was predicted to be 4.98%. The physicochemical properties, in vitro antioxidant activities, and inhibitory effects on α-glucosidase of fractionated TFPPs (TFPP-0, TFPP-20, TFPP-40, and TFPP-60) were investigated. We found that the TFPPs were all acid protein-bound heteropolysaccharides, although with different chemical compositions. They had not only remarkable scavenging activity on 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) and reducing activity, but also excellent inhibitory potential against α-glucosidase in vitro. Our results suggest that tea fruit peel could be treated as a potential bioresource for the development of polysaccharide antioxidants. 相似文献
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为探讨五禽戏对老年人机体抗氧化活性影响的规律及其生理学机制,对长期练习五禽戏的老年人选行抗氧化能力和肠道乳酸菌的测试.结果表明,与五禽戏练习前相比,练习2个月、4个月时,血清MDA水平明显下降,GSH水平、SOD、CAT、GSH-Px和GR活性以及肠道乳酸菌数量明显上升,且均没有显著性别差异,提示五禽戏练习可以明显提高老年人机体的抗氧化能力和肠道乳酸菌的数量,促进机体健康.乳酸菌通过产生SOD、GSH和还原性物质等,提高机体抗氧化能力,可能是五禽戏练习使老年人抗氧化能力增强的生理学机制之一. 相似文献