| 蚓激酶基因克隆及序列分析 |
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| 引用本文： | 姜琼,陈武.蚓激酶基因克隆及序列分析[J].宜春学院学报,2005,27(2):60-62. |
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| 作者姓名： | 姜琼 陈武 |
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| 作者单位： | 宜春学院生物工程研究所,江西,宜春,336000 |
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| 基金项目： | 江西省教育厅计划资助项目 |
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| 摘 要： | 目的：克隆蚓激酶基因并在GENEBANK中进行序列分析．方法：采用RT—PCR技术，以蚯蚓总．RNA为模板进行扩增，克隆蚓激酶基因、用Blast软件对基因进行同源性分析．结果：克隆了一个cDNA片段，与GENEBANK中蚓激酶基因序列最高同源性为99％．结论：本方法实用可行，同源性分析表明克隆的cDNA片段具备完整的蚓激酶编码区，为下一步进行蚓激酶的表达研究奠定了良好的基础．
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| 关 键 词： | 蚓激酶 同源性分析 RT—PCR |
| 文章编号： | 1671-380X(2005)02-0060-03 |
| 修稿时间： | 2005年3月7日 |
Cloning and Sequencing of Lumbrokinase Gene |
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| Jiang Qiong,CHUN Wu.Cloning and Sequencing of Lumbrokinase Gene[J].Journal of Yichun University,2005,27(2):60-62. |
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| Authors: | Jiang Qiong CHUN Wu |
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| Institution: | Yichun University Institute of Bioengineering. Yichun Jiangxi 336000 China |
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| Abstract: | Objective: To clone lumbrokinase gene and undertake sequence analyze in GENEBANK. Methods: Adopting RT-PCR techniques to amplify lumbrokinase gene using the total RNA of earthworm as template. Results: cDNA fragment were amplified and then cloned. After sequencing and analyzing the sequences by Blast, we find the highest sequence similarity was up to 99% between the sequence we submitted and that of lumbrokinase gene exited in GENEBANK. Conclusion: The method is feasible and the sequence analyzing have demonstrated that the two cDNA fragment have the whole coding sequence of lumbrokinase, which laid a good foundation for the study of the expression of lumbrokinase. |
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| Keywords: | lumbrokinase sequence analyze RT-PCR |
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