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1.
Objective: To observe the effect of Yangxueqingnao particles on rat vascular smooth muscle cell (VSMC) proliferation induced
by lysophosphatidic acid (LPA). Methods: The amount of3H-TdR (3H-thymidine) admixed in cultured rat VSMC was measured and mitogen-activated protein kinase (MAPK) activity and lipid peroxidation
end product malondialdehyde (MDA) content of the VSMC were assayed. Results: 1×10−9, 1×10−8, 1×10−7 mol/L LPA in a concentration dependent manner, induced the amount of3H-TdR admixed, MAP kinase activity, and MDA content of the cultured rat VSMC to increase. However, 5%, 10%, and 15% Yangxueqingnao
serum preincubation resulted in a decrease of 23.0%, 42.0%, and 52.0% (P<0.01) respectively in the amount of3H-TdR admixed, a decline in VSMC MAP kinase activity of 13.9% (P<0.05), 29.6% (P<0.01), and 48.9% (P<0.01) respectively, and also, a decrease in MDA content of VSMC of 19.4%, 24.7%, and 43.2% (P<0.01) respectively, in the 1×10−7 mol/L LPA-treated VSMC. Conclusions: LPA activates the proliferation and lipid peroxidation of VSMC in a concentration dependent
manner. The LPA-induced VSMC proliferation is related to the activity of MAP kinases, enzymes involved in an intracellular
signalling pathway. The results of the present study showed that Yangxueqingnao particles can effectively inhibit LPA-induced
VSMC proliferation, MAP kinase activation, and reduce lipid peroxidative lesion.
Project (No. 491010-W50339) supported by Chinese Traditional Medicine Administration Bureau of Zhejiang Province, China 相似文献
2.
目的:观察黄芩甙注射液对四氯化碳(CCl4)致急性肝损伤小鼠肝抗氧化功能的影响。方法:50只小鼠随机分为5组,每组10只,分别为对照组、模型组、黄芩甙注射液低、中、高(0.25 g/kg、0.50 g/kg、1.00g/kg)剂量组。用CCl4制备小鼠急性肝损伤模型,测定小鼠体重变化,肝指数,肝超氧化物歧化酶(SOD)及谷胱甘肽过氧化物酶(GSH-Px)活性,谷胱甘肽(GSH)及丙二醛(MDA)含量。结果:与对照组及模型组比较,黄芩甙各剂量组小鼠试验前后的体重及肝指数差异不显著(P>0.05)。与模型组比较,低剂量组小鼠肝SOD、GSH-Px活性及MDA含量差异不显著(P>0.05),而GSH含量显著升高(P<0.05);中、高剂量组SOD、GSH-Px活性极显著提高(P<0.01),GSH含量极显著升高(P<0.01),MDA含量极显著降低(P<0.01)。结论:黄芩甙能显著增强急性肝损伤小鼠肝SOD、GSH-Px活性,提高GSH含量,降低MDA含量,并表现正向的量效关系。 相似文献
3.
Shao-xiang Weng Mei-hua Sui Shan Chen Jian-an Wang Geng Xu Ji Ma Jiang Shan Lu Fang 《Journal of Zhejiang University. Science. B》2009,10(7):528-535
Objective This study is to determine the effect of the natural product parthenolide, a sesquiterpene lactone isolated from extracts
of the herb Tanacetum parthenium, on the proliferation of vascular smooth muscle cells (VSMCs).
Methods Rat aortic VSMCs were isolated and cultured in vitro, and treated with different concentrations of parthenolide (10, 20 and
30 μmol/L). [3H]thymidine incorporation was used as an index of cell proliferation. Cell cycle progression and distribution were determined
by flow cytometric analysis. Furthermore, the expression of several regulatory proteins relevant to VSMC proliferation including
IκBα, cyclooxygenase-2 (Cox-2), p21, and p27 was examined to investigate the potential molecular mechanism.
Results Treatment with parthenolide significantly decreased the [3H]thymidine incorporation into DNA by 30%∼56% relative to control values in a dose-dependent manner (P<0.05). Addition of parthenolide also increased cell population at G0/G1 phase by 19.2%∼65.7% (P<0.05) and decreased cell population at S phase by 50.7%∼84.8% (P<0.05), which is consistent with its stimulatory effects on p21 and p27. In addition, parthenolide also increased IκBα expression
and reduced Cox-2 expression in a time-dependent manner.
Conclusion Our results show that parthenolide significantly inhibits the VSMC proliferation by inducing G0/G1 cell cycle arrest. IκBα and Cox-2 are likely involved in such inhibitory effect of parthenolide on VSMC proliferation. These
findings warrant further investigation on potential therapeutic implications of parthenolide on VSMC proliferation in vivo.
Project (No. 491020-W50315) supported by the Foundation of the Health Bureau of Zhejiang, China 相似文献
4.
Kang-ting Ji Jun-de Chai Cheng Xing Jin-liang Nan Peng-lin Yang Ji-fei Tang 《Journal of Zhejiang University. Science. B》2010,11(8):618-626
In this study,we examined the protective effects of Danshen both on endothelial progenitor cells(EPCs) in patients with hypercholesterolemia and on in-vitro EPCs of healthy volunteers.In the clinical study,we randomly divided 24 subjects with hypercholesterolemia into two groups(the control group and the Danshen-treated group).At the end of two weeks of treatment,the EPC cellular functions of both groups were tested.The results indicated that,compared to the control group,EPCs in the Danshen-treated group showed significantly better cellular functions,which was manifested in the cloning number,the proliferation capacity,the number of EPC adhesions,and cell migration.In the subsequent in-vitro experiments,EPCs were treated with vehicle,oxidized low-density lipoprotein(Ox-LDL,100 μg/ml),or Ox-LDL(100 μg/ml) plus different concentrations of Danshen(Danshensu 2,10,or 50 μg/ml,respectively) for 24 h.The results showed that Danshen treatments can prevent the detrimental effects of Ox-LDL on EPC cellular functions measured by proliferation capacity(0.24±0.08,0.37±0.11,0.30±0.04 vs.0.13±0.02,P0.05,P0.01,and P0.01,respectively),and adhesion ability(63.00±11.60,70.00±10.80,85.50±11.41 vs.40.50±6.85,all P0.01).Compared to the group treated with Ox-LDL alone,Danshen treatment significantly decreased the lipid peroxidation end product malondialdehyde(MDA) [(4.34±0.54),(3.98±0.47),(3.46±0.31) vs.(5.57±0.64) nmol/ml,all P0.01],increased the production of superoxide dismutase(SOD) [(29.74±0.71),(31.09±0.83),(30.41±0.65) vs.(14.76±3.99) U/ml,all P0.01],and lowered the expression of interleukin-6(IL-6) [(24.62±7.69),(27.04±3.14),(33.38±18.86) vs.(230.67±33.53) pg/ml,all P0.01] and tumor necrosis factor-α(TNF-α) [(41.72±6.10),(17.02±6.82),(3.73±2.26) vs.(228.71±41.53) pg/ml,all P0.01] in Ox-LDL treated EPCs.These results suggest that Danshen may exert a protective effect through its antioxidant and anti-inflammatory features. 相似文献
5.
针剌手厥阴心包经穴对力竭大鼠心肌过氧化损伤的保护作用 总被引：1，自引：0，他引：1
目的:观察针刺手厥阴心包经穴对大鼠自由基代谢的影响,探讨针灸对力竭运动心脏的保护作用.方法:24只SD大鼠随机分为安静对照组、力竭运动组、针刺力竭运动组.针刺力竭运动组每日针刺双侧内关穴和郄门穴一次,连续20天后,力竭运动组、针刺力竭运动组做力竭游泳实验.运动后即刻处死各组大鼠,取心脏备用.分别测定其心肌线粒体SOD活性、GSH和MDA含量.结果:力竭运动组与安静对照组相比,GSH、SOD下降明显(P<0.01),而MDA显著升高(P<0.01).针刺力竭运动组MDA明显低于力竭运动组(P<0.01),SOD显著高于力竭运动组(P<0.01),GSH略高于力竭运动组,无统计学意义.结论:针刺内关、郄门穴能增强心肌细胞清除自由基能力,抑制力竭运动时心肌脂质过氧化反应而起到保护心肌的作用. 相似文献
6.
Effects of hypoxic exercise training on microRNA expression and lipid metabolism in obese rat livers
Ying-li Lu Wen Jing Lian-shi Feng Li Zhang Jian-fang Xu Tong-jian You Jing Zhao 《Journal of Zhejiang University. Science. B》2014,15(9):820-829
To investigate the effects of hypoxic exercise training on microRNA (miRNA) expression and the role of miRNA expression in regulating lipid metabolism, 20 dietary-induced obese SD rats were divided into a normoxic sedentary group (N, n=10) and a hypoxic exercise training group (H, n=10). After four weeks, measurements were taken of body weight, body length, fat mass, serum lipid concentration, miRNAs differentially expressed in rat liver, and gene and protein expression levels of peroxisome proliferator activated receptor α (PPARα), fatty acid synthetase (FAS), and carnitine palmitoyl transferase 1A (CPT1A) in rat liver. Body weight, Lee’s index, fat mass, fat/weight ratio, and serum levels of total cholesterol (TC) and high density lipoprotein cholesterol (HDL-C) were all significantly lower in the H group than in the N group (P<0.01). Six miRNAs expressed significantly differently in the liver (P<0.05). Specifically, expression levels of miR-378b were significantly lower in the H group than in the N group (P<0.05). Compared with the normoxic sedentary group, hypoxic exercise training resulted in a lower ratio of FAS mRNA to CPT1A mRNA (P<0.05), as well as lower CPT1A protein levels (P<0.01), while a higher ratio of FAS to CPT1A protein levels (P<0.01) was observed. In conclusion, hypoxic training may elevate the resistance of high fat diet induced obesity in rats by reducing the expression of miR-378b, and decrease the fatty acid mitochondrial oxidation in obese rat livers by decreasing the protein expression of CPT1A and increasing the protein expression ratio of FAS/CPT1A. 相似文献
7.
目的:观察悦容丹内服治疗黄褐斑的临床疗效,重点探讨其对患者血清超氧化物歧化酶(SOD)、丙二醛(MDA)水平的影响。方法:80例黄褐斑患者随机分成治疗组(40例)、对照组(40例),正常组(38例)。治疗组采用悦容丹口服加外用广谱防晒霜,对照组维生素C片和维生素E胶丸口服加外用广谱防晒霜,治疗12周,正常组不治疗。观察分析临床疗效及不良反应。结果:治疗组有效率95.00%,对照组有效率72.5%,两组相比较有统计学意义(P<0.05);较正常组,治疗组与对照组SOD显著升高、MDA显著降低,差异有显著性(P<0.05);治疗组血清SOD水平治疗后明显高于治疗前,MDA水平治疗后明显低于治疗前,差异有统计学意义(P<0.01);治疗后,治疗组血清SOD、MDA水平改善程度明显优于对照组(P<0.05)。结论:悦容丹对黄褐斑疗效显著,无不良反应。其机制与调控机体SOD、MDA水平有一定相关性,值得临床深入探讨。 相似文献
8.
目的:探讨乙型肝炎患者机体抗氧化能力的变化及其与病情的关系.方法: 门诊乙型肝炎患者共124例和健康者30例,均经放射免疫方法检测后加以确诊.乙型肝炎患者分为三组:乙型肝炎病毒复制组、乙型肝炎病毒恢复组、乙型肝炎合并肝癌组.患者组和对照组均经7150型全自动生化分析仪测定血清ALT、AST含量,应用改良TBA微量法测定血清丙二醛(MDA),以黄嘌呤氧化酶法测定超氧化物歧化酶(SOD)、Cu/Zn-SOD及Mn-SOD水平的变化.结果: 乙型肝炎病毒复制组、乙型肝炎病毒恢复组、乙型肝炎合并肝癌组三组患者血清MDA水平均显著高于对照组(P<0.01),而血清SOD、Cu/Zn-SOD及Mn-SOD水平均显著低于对照组(P<0.01);乙型肝炎病毒复制组、乙型肝炎合并肝癌组患者血清MDA水平均显著高于乙型肝炎病毒恢复组(P<0.01),而血清SOD、Cu/Zn-SOD及Mn-SOD水平均显著低于乙型肝炎病毒恢复组(P<0.01);乙型肝炎合并肝癌组患者血清MDA水平均显著高于乙型肝炎病毒复制组(P<0.01),而血清SOD、Cu/Zn-SOD及Mn-SOD水平均显著低于乙型肝炎病毒复制组(P<0.01).但血清MDA水平与ALT、AST水平(r=0.151),血清SOD、Cu/Zn-SOD及Mn-SOD水平与ALT、AST水平(r=-0.218)均无相关性(P>0.05).结论: 乙型肝炎患者出现明显抗氧化能力的异常.通过血清MDA、SOD、Cu/Zn-SOD及Mn-SOD水平的检测,可为乙型肝炎的临床防治及疗效观察提供一定的依据. 相似文献
9.
目的以衰老的自由基学说为依据,研究蚁皇浆口服液的抗衰老作用.方法52周龄小白鼠30只随机分为老龄实验组和老龄对照组,4周龄15只作为成年对照组,灌胃四周后分别取血测定SOD、CAT、Tch、TG和MDA等各项生化指标.结果老龄实验组SOD、CAT活性均明显升高(P＜0.01),MDA、Tch和TG含量明显降低(P＜0.01或P＜0.05).结论蚁皇浆口服液具有较强的抗氧化和抗衰老作用,是一种很有经济价值药食两用的保健品. 相似文献
10.
目的:探索肝癌患者血清SOD、MDA的变化。对肝癌患者26例和健康对照组30例。方法:对肝癌患者均应用改良TBA微量法测定血清丙二醛(MDA)、以黄嘌呤氧化酶法测定超氧化物歧化酶(SOD)水平的变化。结果:肝癌组患者血清MDA水平显著高于对照组(P<0.01),而血清SOD水平显著低于对照组(P<0.01)。结论:提示肝癌患者存在明显的抗氧化能力障碍,通过血清MDA、SOD水平的检测,可为肝癌的临床治疗及疗效观察提供一定的依据。 相似文献
11.
目的探讨CD40-CD40配体相互作用对体外培养的人脐静脉内皮细胞(HUVEC)增殖及迁移的影响。方法采用Ⅱ型胶原酶消化法培养人脐静脉内皮细胞,以3H-胸腺嘧啶脱氧核苷(3H-TdR)、3H-亮氨酸(3H-Leu)掺入法分别测定HUVEC增殖,内皮细胞的迁移采用琼脂糖凝胶刮取法,倒置显微镜观察。结果CD40-CD40配体相互作用能明显促进HUVEC3H-TdR、3H-Leu的掺入,两者具有时间、剂量依赖性,CD40-CD40配体相互作用随CD40L浓度的增加及刺激时间延长(30h内),能明显增加内皮细胞迁移率,an-ti-CD40单克隆抗体能明显抑制上述效应。结论CD40-CD40配体相互作用能促进内皮细胞增殖、迁移。 相似文献
12.
贾文娟 《思茅师范高等专科学校学报》2013,(6):13-18
目的：通过实验观察甲醛对运动大鼠心肌组织抗氧化系统的影响及服用刺五加后的作用,探讨甲醛对运动大鼠的心肌组织毒性及刺五加的保护作用。方法：采用人为模拟不同程度甲醛染毒环境进行动式染毒,甲醛剂量分别为0.8 mg/m3,2.4 mg/m3。每天染毒30 min,每周6次,连续4周,刺五加的灌胃剂量为104.17 mg/kg,测定运动大鼠心肌组织的超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-PX）、过氧化氢酶（CAT）的活力和丙二醛（MDA）的含量。结果：染毒组与对照组相比,2.4 mg/m3剂量染毒运动组大鼠心肌组织 CAT、GSH-PX、SOD活性明显低于对照组（P〈0.01）,MDA含量明显高于对照组（P〈0.01）。服药染毒运动组与染毒运动组相比,0.8mg/m3剂量染毒服药组大鼠心肌组织CAT、GSH-PX、SOD活性明显高于染毒运动组（P〈0.01）,MDA含量明显低于染毒运动组（P〈0.01）。结论：甲醛可以降低染毒大鼠心肌组织抗氧化酶的活性,造成心肌组织氧化性损伤,而灌服中药刺五加可以有效缓解其毒性。 相似文献
13.
目的：观察急性肾功能衰竭（ARF）家兔心肌匀浆自由基、一氧化氮的变化，探讨心肌损伤的体液机制。方法：60只家兔均分为四组（n=15）。ARF模型1组：皮下注射1％HgCl2（1．3ml／kg．bw）；ARF模型2组：肌肉注射50％甘油（10ml／kg．bw）；以等量生理盐水代替HgCl2和甘油作为对照1、2组。24h后，所有动物颈总动脉放血备检，并选择固定位置。制备10％心肌匀浆。经Aeroset型全自动生化分析仪测定血清反映肾功能的生化指标。检测心肌匀浆丙二醛（MDA）、超氧化物歧化酶（SOD）、一氧化氮（NO）及其合酶（NOS）的变化。结果：与相应对照组比较，ARF模型1、2组心匀浆SOD活性下降、MDA含量升高（P〈0．05），ARF模型1、2组心匀浆NO含量增强、NOS及iNOS活性增强（P〈0．05～0．01）。结论：ARF家兔心肌损伤的机制与自由基损伤及NO升高有关。 相似文献
14.
Objective： To investigate the dynamics of vascular volume and the plasma dilution of lactated Ringer＇s solution in patients during the induction of general and epidural anesthesia. Methods： The hemodilution of i.v. infusion of 1000 ml of lactated Ringer＇s solution over 60 min was studied in patients undergoing general （n=31） and epidural （n= 22） anesthesia. Heart rate, arterial blood pressure and hemoglobin （Hb） concentration were measured every 5 rain during the study. Surgery was not started until the study period had been completed. Results： General anesthesia caused the greater decrease of mean arterial blood pressure （MAP） （mean 15% versus 9%; P〈0.01） and thereby followed by a more pronounced plasma dilution, blood volume expansion （VE） and blood volume expansion efficiency （VEE）. A strong linear correlation between hemodilution and the reduction in MAP （r=-0.50;P〈0.01） was found. At the end of infusion, patients undergoing general anesthesia retained 47% （SD 19%） of the infused fluid in the circulation, while epidural anesthesia retained 29% （SD 13%） （P〈0.001）. Correspondingly, a fewer urine output （mean 89 ml versus 156 ml; P〈0.05） and extravascular expansion （454 ml versus 551 ml; P〈0.05） were found during general anesthesia. Conclusion： We concluded that the induction of general anesthesia caused more hemodilution, volume expansion and volume expansion efficiency than epidural anesthesia, which was triggered only by the lower MAP. 相似文献
15.
目的:探讨甲状腺功能亢进对机体抗氧化能力的影响。方法:应用放射免疫方法(RIA)对甲亢患者与正常人进行了甲状腺功能的测定,同时用改良TBA微量法测定血清丙二醛(MDA)水平、黄嘌呤氧化酶法测定超氧化物歧化酶(SOD)活性的变化,进行相关性分析。结果:甲亢组MDA水平显著高于对照组(P<0.01),SOD水平显著低于对照组(P<0.01);血清MDA、SOD水平与T_3、T_4无线性相关关系。结论:甲亢患者机体有明显的抗氧化能力障碍,血清MDA、SOD水平可作为甲亢患者临床防治及疗效观察的指标。 相似文献
16.
复方中草药对AA肉鸡肌肉抗氧化能力的影响 总被引：1，自引：0，他引：1
目的:研究复方中草药对肉鸡肌肉抗氧化能力的影响。方法:选用健康1日龄AA肉鸡400羽,随机分为对照组和试验Ⅰ、Ⅱ、Ⅲ组,每组100羽。对照组饲喂玉米-豆粕基础日粮,试验Ⅰ、Ⅱ、Ⅲ组分别在基础日粮中添加0.5%、1.0%和1.5%的复方中草药,试验期42 d。结果:21日龄时,与对照组相比,试验Ⅰ、Ⅱ、Ⅲ组肉鸡胸肌和腿肌SOD和T-AOC活性及GSH-PX含量不同程度升高,MDA含量不同程度降低,其中试验Ⅱ组胸肌T-AOC活性和GSH-PX含量显著升高,胸肌MDA含量显著降低(P0.05);试验Ⅰ和Ⅲ组胸肌SOD活性和试验Ⅲ组腿肌SOD活性和GSH-PX含量也显著升高(P0.05);而各试验组腿肌MDA含量均显著降低(P0.05)。42日龄时,与对照组相比,各试验组胸肌MDA含量均显著降低(P0.05),而试验Ⅱ组胸肌GSH-PX含量显著升高(P0.05),且各试验组腿肌T-AOC活性也均有不同程度的提高。结论:添加不同剂量的复方中草药可不同程度提高肉鸡胸肌和腿肌抗氧化酶活性,降低MDA含量,其中以添加1.0%的复方中草药提高胸肌抗氧化能力效果最好,而添加1.5%的复方中草药提高腿肌抗氧化能力效果较好。 相似文献
17.
中华猕猴桃果实成熟过程中膜脂过氧化变化研究 总被引：3，自引：0，他引：3
研究中华猕猴桃果实成熟过程中脂过氧化的变化，结果表明：MDA含量呈上升趋势，SOD、Vc含量呈下降趋势。SOD、POD酶活性出现二次高峰，SOD、POD活性第一次峰出现时，果实内MDA含量下降；第二次峰出现时MDA含量变化不大，两品种比较，果实内Vc含量高，膜脂过氧化水平低时，果实成熟慢。 相似文献
18.
目的:研究溴氰菊酯(DM)对SD大鼠小脑皮质组织中血红素加氧酶(HO)的活性和ATPase活性的影响。方法:(1)成年雄性SD大鼠共40只,其中20只用于血红素加氧酶(HO)的活性的测定,另20只用于ATPase活力测定,均随机分为对照组和DM染毒实验组,实验组中大鼠72小时内给溴氰菊酯(1/20LD50)三次,对照组为相同剂量的玉米油,分别检测小脑皮质组织HO活性和ATPase活性。(2)成年雄性SD大鼠30只,同样方法取出小脑皮质,制成切片,随机分为对照组(相同剂量的玉米油)和5个不同浓度的DM染毒试验组(2×10-7mmol/L、2×10-6mmol/L、2×10-5mmol/L、2×10-4mmol/L、2×10-3mmol/L),测定ATPase活性。结果:整体染毒小脑皮层组织HO活性为对照组的240.39%(p﹤0.05),Na+,K+-ATPase活性为对照组的131.47%(p﹤0.05),Ca2+-ATPase活性增加没有统计学意义,Mg2+-ATPase有显著的抑制作用(P<0.05),为对照组的79.91%。体外试验发现只有达到10-3mmol/L数量级的DM对温育小脑皮质切片组织中的Mg2+-ATPase活性才有显著的抑制作用(P<0.05);且无论何种浓度均未发现上述体内染毒时对Na+,K+-ATPase活性的增加作用。结论:DM可增加大鼠小脑皮质的HO活性,体内体外染毒DM对三种ATPase活性的影响并不一致。 相似文献
19.
目的:探讨沙参麦冬汤对慢性支气管炎大鼠肺、脑组织抗氧化能力的影响.方法:70只SD大鼠,随机分为对照组、慢支模型组、沙参麦冬汤高、低剂量组和氨茶碱组,采用混合烟熏方法复制慢性支气管炎大鼠动物模型,对照组与慢支模型组灌服生理盐水10 mL/kg·d,高剂量组灌服沙参麦冬汤10 mL/kg·d,低剂量组灌服沙参麦冬汤5 mL/kg·d,氨茶碱组灌服氨茶碱0.25 g/kg·d,灌胃期30 d.实验结束后,测定体重和肺指数,测定肺、脑组织中的超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、过氧化氢(CAT)的活力和丙二醛(MDA)的含量.结果:各组的肺指数差异均不显著(P>0.05),沙参麦冬汤对慢支大鼠肺、脑的CAT活性无明显影响(P>0.05),但能显著提高肺、脑组织中SOD,GSH-Px的活性(P<0.05),显著降低肺、脑MDA的含量(P<0.05).结论:沙参麦冬汤可以提高慢支大鼠肺、脑组织的抗氧化能力,降低烟雾造成的氧化损伤. 相似文献
20.
海藻多糖抗氧化作用的实验研究 总被引：1，自引：0，他引：1
目的:研究海藻多糖(Polysaechrides from Spirulina Platensis,PSP)对小鼠肝损伤动物模型与肝匀浆脂质过氧化模型的抗氧化作用。方法:采用腹腔注射CCL4的方法,制造小鼠肝损伤动物模型,金氏法测定血清谷氨酸丙酮酸转氨酶(SGPT)和肝脏的过氧化脂质(Lipoper-oxides,LPO)含量;Fenton反应诱导小鼠肝匀浆脂质过氧化动物模型,采用硫代巴比妥酸(TBA)法测定肝匀浆LPO。结果:在体外,海藻多糖可剂量依赖性地抑制Fenton反应导致的CCL4毒化小鼠肝匀浆中LPO的生成,其IC50为1.6×10-4。在体内,灌胃250mg/kg的海藻多糖组可明显降低CCL4毒化小鼠血清SGPT活性(P<0.01)。结论:海藻多糖对体内外的肝组织有一定程度的抗氧化作用。 相似文献