共查询到20条相似文献，搜索用时 152 毫秒
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《实验室研究与探索》2020,(6)
为了将信号分子NO的检测探针应用于细胞生物学实验,使用NO荧光探针DAF-FM双乙酸盐和小鼠腹腔巨噬细胞,设计了"荧光探针检测巨噬细胞中NO含量"的实验项目,并在本科生细胞生物学实验课中实施。得到一定浓度脂多糖和食用菌多糖处理可显著增强巨噬细胞内NO荧光探针平均光密度值(P <0.05)的实验结果。实验表明,NO荧光探针能直观显示细胞中NO合成量的变化,可作为一种简便和可视化的信号分子检测方法,应用于细胞生物学实验教学。 相似文献
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荧光探针是科学研究的一种新型工具,具有特异性强、灵敏度高和生物相容性好等特点,应用于众多生产生活领域。介绍了荧光探针的结构、分类及反应机理,综述了荧光探针在食品领域及生物学领域中的应用研究进展。主要从食品重金属检测、食物新鲜度检测、细胞活度检测、细胞内活性物质检测等方面介绍了荧光探针的应用实例,并展望其发展前景,旨在为今后荧光探针类高附加值产品的开发与设计提供可行性参考。 相似文献
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《实验技术与管理》2015,(12):66-68
目的:构建稳定表达miR-17-5p的PC12细胞株。方法:将质粒PEGFP-N1-vector空载体和PEGFPN1-miR-17-5p表达载体扩增、抽提,利用脂质体转染技术将质粒转染PC12细胞,荧光显微镜观察绿色荧光蛋白GFP表达,Real-time PCR检测转染PC12细胞miR-17-5p的表达。结果:荧光显微镜观察到空载组和miR-17-5p组细胞都有绿色荧光蛋白高表达,Real-time PCR检测稳定转染miR 17-5p后PC12细胞内miR-17-5p的表达较空载组明显升高(P0.01)。结论:miR-17-5p在PC12细胞稳定表达,为进一步研究miR-17-5p对神经细胞的调制作用提供有用的细胞模型。 相似文献
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本文从s wiss-prot中选取经过试验验证的水稻蛋白质磷酸化位点数据作为训练集合,应用蛋白质序列的氨基酸频率计算方法来进行特征提取,再利用SVM算法构建专门针对水稻蛋白质磷酸化位点的预测新工具.氨基酸频率算法指的是计算出相应待预测磷酸化位点附近氨基酸的出现频率,进一步反映了残基之间的相关性.本文利用LibSVM软件包对已通过氨基酸频率算法特征提取出来的数值特征对磷酸化位点进行预测,从而为之后构建水稻蛋白质磷酸化位点的预测工具做准备.结果表明,本文基于SVM和氨基酸频率方法的水稻蛋白质磷酸化位点预测在丝氨酸,苏氨酸和酪氨酸的平均预测准确性为77.665%,马修斯系数为0.571.与PlantPhos和Musite的预测性能的对比结果显示,在磷酸化苏氨酸位点的预测性能显著高于PlantPhos及Musite. 相似文献
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研究了没食子儿茶素没食子酸酯（EGCG）对人肝癌细胞BEL-7402增殖凋亡及对信号转导蛋白和转录激活因子3（Stat3）蛋白及磷酸化Stat3蛋白表达的影响.应用MTT法检测不同浓度EGCG对BEL-7402细胞增殖的抑制作用,用流式细胞仪分析细胞的凋亡率,用ELISA方法检测EGCG对Stat3蛋白及磷酸化的Stat3蛋白表达的影响.结果表明：EGCG有抑制肝癌细胞增殖促进凋亡的作用,并呈剂量依赖性.ELISA结果显示EGCG能降低磷酸化Stat3蛋白的表达水平.EGCG能抑制肝癌细胞的增殖促进凋亡,其机制可能有与下调磷酸化的Stat3蛋白的表达有关. 相似文献
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为研究牛源抗菌肽乳铁素的生物学功能,通过网络在线软件和工具分析了牛源抗菌肽乳铁素的生物信息学相关特性.结果表明:牛源抗菌肽乳铁素为不稳定的、带正电荷的亲水性蛋白多肽,没有跨膜区和信号肽,也没有糖基化位点和磷酸化位点,二级结构主要由α-螺旋和无规则卷曲构成,呈环形结构,定位于细胞外,可与19个蛋白分子发生相互作用.这为深入理解牛源抗菌肽乳铁素的生物学功能奠定了基础. 相似文献
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共聚焦激光扫描显微镜(Confocal Laser ScanningMicroscope,CLSM)是上世纪80年代发展起来的一种先进的细胞生物学分析仪器,是一项具有划时代意义的高科技新产品,是近代生物医学图像分析仪器最重要的发展之一,有细胞"CT"之称.它是在荧光显微镜成像基础上加装了激光扫描装置,利用计算机进行图像处理,使用紫外线或可见光激发荧光探针,从而得到组织内部微细结构的荧光图像,在亚细胞水平上观察诸如Ca2 ,pH值、膜电位等生理信号及细胞形态改变,成为形态学、分子细胞生物学、神经科学、药理学、遗传学等领域中新一代较有力的研究工具. 相似文献
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《赣南师范学院学报》2015,(6):39-43
根据间接设计荧光探针的方法,设计合成了一种带有2,2’-二甲基吡啶胺结构(DPA)的卟啉化合物(TPP-DPA),其选择性识别二价铜离子形成的络合物可以作为一种识别α-氨基酸的"Turn On"型的荧光探针.TPP-D PA在DMSO中强的荧光可以被少量的Cu(2+)有效的猝灭,所形成的(TPP-DPA+Cu(2+)有效的猝灭,所形成的(TPP-DPA+Cu(2+))络合物可以进一步作为识别α-氨基酸的荧光探针,尤其对含有巯基的α-氨基酸(如半胱氨酸)有高灵敏度和选择性. 相似文献
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Peng-yan QIAO Fang-fang LI Li-min DONG Tao XU Qiu-fei XIE 《Journal of Zhejiang University. Science. B》2014,(4)
研究目的:本研究应用海藻酸钠-壳聚糖微囊保护成骨细胞,接种到β-磷酸三钙/磷酸钙骨水泥(β-TCP/CPC)浆料中,使β-TCP/CPC骨修复材料具有一定的细胞活性,同时提高固化后材料的孔隙率和孔径,以最终实现提高β-TCP/CPC骨水泥的降解速度,加快成骨和骨修复。创新要点:本研究首次应用海藻酸钠-壳聚糖微胶囊包封成骨细胞与CPC浆料复合,复合后实现自动细胞释放,释放出的细胞具有良好的生物学活性。研究方法:(1)高压静电成囊法制备载小鼠成骨前体细胞(MC3T3-E1)的海藻酸钙和海藻酸钠-壳聚糖微胶囊;(2)微囊化MC3T3-E1细胞,进行体外培养,使用细胞计数试剂盒(CCK-8)检测细胞活性,并用钙黄绿素-AM(Calcein-AM)和碘化丙啶(PI)进行活死细胞双重染色;(3)微囊化MC3T3-E1细胞与β-TCP/CPC浆料复合培养后,激光共聚焦扫描显微镜和环境扫描电子显微镜观测细胞在材料上的释放、粘附,CCK-8法检测材料上细胞的活力,碱性磷酸酶(ALP)检测观察细胞的分化状况,茜素红染色观察释放细胞的矿化能力。重要结论:海藻酸钠-壳聚糖微胶囊可作为可注射磷酸钙骨水泥内部接种成骨细胞并实现细胞释放的良好载体,释放出的成骨细胞具有良好的生物学活性。 相似文献
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Xiao-li Xie Li-fei Zheng Ying Yu Li-ping Liang Man-cai Guo John Song Zhi-fa Yuan 《Journal of Zhejiang University. Science. B》2012,13(2):152-158
Biology sequence comparison is a fundamental task in computational biology. According to the hydropathy profile of amino acids,
a protein sequence is taken as a string with three letters. Three curves of the new protein sequence were defined to describe
the protein sequence. A new method to analyze the similarity/dissimilarity of protein sequence was proposed based on the conditional
probability of the protein sequence. Finally, the protein sequences of ND6 (NADH dehydrogenase subunit 6) protein of eight
species were taken as an example to illustrate the new approach. The results demonstrated that the method is convenient and
efficient. 相似文献
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Kathleen M. Fisher 《科学教学研究杂志》1985,22(1):53-62
A persistent error has been observed among students in introductory biology and genetics. In interviews and examinations, many students express the belief that amino acids are produced by genetic translation (protein synthesis). The evidence suggests that at least four underlying factors contribute to error occurrence: (1) strong word association between the terms “amino acids” and “proteins”, (2) confusion resulting from familiar and unfamiliar levels of generality and specificity, (3) conflict resulting from the dual roles of some proteins as participants in and products of translation, and (4) lack of knowledge about the actual origins of amino acids in cells. Implications for teaching are discussed. 相似文献
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Hurd DD 《CBE life sciences education》2008,7(2):210-219
The skill set required of biomedical researchers continues to grow and evolve as biology matures as a natural science. Science necessitates creative yet critical thinking, persuasive communication skills, purposeful use of time, and adeptness at the laboratory bench. Teaching these skills can be effectively accomplished in an inquiry-based, active-learning environment at a primarily undergraduate institution. Cell Biology Techniques, an upper-level cell biology laboratory course at St. John Fisher College, features two independent projects that take advantage of the biology of the nematode Caenorhabditis elegans, a premier yet simple model organism. First, students perform a miniature epigenetic screen for novel phenotypes using RNA interference. The results of this screen combined with literature research direct students toward a singe gene that they attempt to subclone in the second project. The biology of the chosen gene/protein also becomes an individualized focal point with respect to the content of the laboratory. Progress toward course goals is evaluated using written, oral, and group-produced assignments, including a concept map. Pre- and postassessment indicates a significant increase in the understanding of broad concepts in cell biological research. 相似文献
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Ghanshyam Swarup 《Resonance》1998,3(10):70-78
Modification of proteins by phosphorylation is the major general mechanism by which many cellular functions in eukaryotic
cells such as cell division, malignant transformation, differentiation, signal transduction etc. are controlled by external
physiological stimuli. At the molecular level protein phosphorylation-dephosphorylation can alter various properties of the
substrate molecules such as enzymatic activity, sub-Cellular location, ligand binding, interaction with other proteins, DNA
binding and some other functional properties. The changes in molecular properties of proteins brought about by protein phosphorylation
play a critical role in regulating various cellular functions. 相似文献
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绿色荧光蛋白及其在分子生物学上的应用 总被引：3，自引：0，他引：3
许多海洋无脊椎动物体内都含有绿色荧光蛋白,这种蛋白质结构很特殊,在受到蓝光或紫外线刺激时可以发射绿色荧光,并不需要任何协同因子、底物,适合用作普遍的报告标记,尤其适合于活体细胞或组织。而且它发出的荧光稳定,检测简单,结果真实可靠。并且GFP对光漂白、氧化剂、还原荆以及其他许多化学试剂具有极强的稳定性．易于构建载体。它独特的性质引起了生物学界的广泛关注。本文简要地概述了绿色荧光蛋白及其在分子生物学上的应用,包括蛋白融合,细胞筛选,定位标记,基因表达调控,计算细胞生长速度等。 相似文献
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Bradley J. Stith 《CBE life sciences education》2004,3(3):181-188
To address the different learning styles of students, and because students can access animation from off-campus computers, the use of digital animation in teaching cell biology has become increasingly popular. Sample processes from cell biology that are more clearly presented in animation than in static illustrations are identified. The value of animation is evaluated on whether the process being taught involves motion, cellular location, or sequential order of numerous events. Computer programs for developing animation and animations associated with cell biology textbooks are reviewed, and links to specific examples of animation are given. Finally, future teaching tools for all fields of biology will increasingly benefit from an expansion of animation to the use of simulation. One purpose of this review is to encourage the widespread use of animations in biology teaching by discussing the nature of digital animation. 相似文献
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作为生命科学以及生物技术专业的必修课,细胞生物学是一门以实验为基础的学科。细胞生物实验教学既是培养学生观察能力和实验能力的重要环节,也是培养学生科学素质的有效途径。从拓展教学手段、丰富教学内容以及完善考核方式三个方面进行了教改探讨,以期提高学生的创新意识和学习兴趣,从而实现培养新世纪复合型人才的目标。 相似文献
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DNA是生物体遗传信息的载体,而其中的螺旋结构具有重要的生物学功能.右手双螺旋DNA结构是其贮存和传递遗传信息的分子基础,调控蛋白通过识别双螺旋沟中的信息调节基因表达;原核细胞的染色体DNA形成的负超螺旋分区结构有利于基因表达时的操纵子的协同调节;真核细胞线状染色体DNA缠绕组蛋白形成的负超螺旋,以及超螺旋环也是基因复制与表达所必需的. 相似文献
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SsrA peptide tag from Mycoplasma florum has been developed as a versatile biotechnology tool to control orthogonal degradation of tagged proteins in Escherichia coli. Here, using the systematic deletion mutants of mf-ssrA tag, we demonstrated that the residues in two separate regions have different functions in mf-Lon-mediated specific orthogonal target protein degradation in E. coli. The deletion of multiple residues, up to six amino acids, did not fatally abolish its specific degradation activity, instead of being able to improve the stability of the tagged protein in the presence of endogenous proteases before mf-Lon expression in E. coli. Except for previously identified essential residues, the region adjacent to the C-terminal of the mf-ssrA tag was involved in mf-Lon and endogenous protease-mediated degradation. Moreover, the deletion of specific residues made the mf-ssrA tag more effective and compact. The mf-ssrA tag can be implemented in synthetic biology and bioengineering for development of synthetic circuits. 相似文献