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1.
STZ诱导的糖尿病对骨密度和血清睾酮的影响（英文） 总被引：2，自引：0，他引：2
目的：探讨链脲霉素（STZ）诱导的糖尿病对大鼠骨代谢的影响及机制。材料与方法：雄性Wistar大鼠随机分成3组: 正常组（n = 6），糖尿病组（n = 5）和胰岛素治疗糖尿病组 (n = 5)。大鼠尾静脉一次性注射STZ（50 mg/kg 体重），选择空腹12 h血糖大于12 mmol/L的大鼠为本实验所需的糖尿病模型。治疗组大鼠每天在同一时间给予1.8－2.2U的胰岛素。实验周期为持续32天。采用双能X线骨吸收法（DEXA）测定股骨密度，ELISA法测定血清雄性激素睾酮水平，生化分析仪测定血清碱性磷酸酶、钙、磷浓度。结果：糖尿病大鼠的股骨密度和血清睾酮均显著低于正常组（P < 0.01），血清总碱性磷酸酶明显高于正常组和胰岛素治疗组（P < 0.01）。三组之间血钙和血磷的水平无明显差异。胰岛素治疗后糖尿病大鼠的骨密度、血清睾酮与总碱性磷酸酶得到明显的改善。结论：糖尿病严重影响骨密度，胰岛素缺乏及雄性激素降低是导致糖尿病性骨质疏松重要原因。胰岛素治疗能预防骨流失和提高血清睾酮浓度。 相似文献
2.
4-氯-2,6-双吡啶双氧钒化合物降低1型糖尿病大鼠血糖的生物学作用 总被引：1，自引：0，他引：1
通过1型和2型糖尿病动物模型的研究发现过渡金属元素钒（V）具有降糖作用。有机钒化合物与无机钒相比，其副作用低、体内吸收率和生物利用度高。本文主要探讨了四价有机钒化合物4-氯-2,6-双吡啶双氧钒化合物（4-chloro-2,6-dipicolinatodioxovanadium(Ⅳ)，V4dipic-Cl）对链脲佐菌素（STZ，55 mg/kg）诱导的1型糖尿病大鼠的生物学作用。大鼠通过自由饮水的方式口服V4dipic-Cl（0.5 mg/ml）8天。结果表明，V4dipic-Cl组的大鼠血糖和血清碱性磷酸酶（ALP）活性显著低于糖尿病对照组，并且其葡萄糖耐量水平得到明显改善。但有机配体H2dipic-Cl对糖尿病大鼠的血糖和血清ALP活性的影响不显著。结果提示V4dipic-Cl具有降低糖尿病大鼠的血糖，提高葡萄糖耐量和改善肝功能的作用。 相似文献
3.
《科技风》2021,(15)
目的:探究microRNA-369-5p(miR-369-5p)在糖尿病心肌病心肌组织与心肌成纤维细胞(cardiac fibroblasts CFs)活化增殖中的表达变化。方法:通过腹腔注射链脲佐菌素(STZ)构建大鼠糖尿病心肌病模型,体外应用高糖诱导CFs细胞活化增殖模型。利用HE、Masson染色,观察心肌组织病理改变及胶原分布情况。应用qRT-PCR检测CFs细胞中miR-369-5p的表达。qRT-PCR和Western blotting实验检测α-SMA、Collagen I mRNA与蛋白的表达;结果:HE和Masson染色显示糖尿病心肌病大鼠心肌胶原沉积明显增多,细胞相对排列紊乱,细胞大小不一。Western blotting实验表明与正常组相比,糖尿病组和高糖组的α-SMA与Collagen I的表达增长;qRT-PCR实验表明与正常组相比,高糖诱导CFs细胞中miR-369-5p表达降低,而α-SMA与Collagen I的表达增加。结论:miR-369-5p在糖尿病心肌病心肌组织与高糖诱导CFs细胞中表达降低,提示miR-369-5p可能是影响糖尿病心肌病形成的潜在作用靶点。 相似文献
4.
目的 利用链脲佐菌素（STZ）联合高脂饮食复制2型糖尿病小鼠模型,观察茶多糖对小鼠血清生化指标的影响.方法 90只小鼠给予高脂饮食4周后腹腔注射STZ建立2型糖尿病模型,随机分成模型组、茶多糖高中低三个剂量组以及阳性药二甲双胍组,另取10只为正常对照组.茶多糖组、阳性对照组分别按照计量灌胃治疗,对照组和模型组给予等体积的蒸馏水灌胃.4周后,检测小鼠血清空腹血糖、胰岛素、甘油三酯、总胆固醇以及糖耐量等指标.结果 4周后,茶多糖中高剂量组空腹血糖、甘油三酯以及总胆固醇均比模型组低（P＜0.05,P＜0.01）,而给药组血清胰岛素水平比模型组高（P＜0.05,P＜0.01）,并且给药组与模型组比较小鼠糖耐量有所改善（P＜0.05,P＜0.01）.结论 茶多糖能够降低2型糖尿病小鼠血糖,并且能够改善脂类代谢. 相似文献
5.
Ⅱ型糖尿病（T2DM）占糖尿病发病率的90%以上，已成为影响全球生命健康的主要慢性疾病之一。开发高效、安全的糖尿病治疗药物一直是该领域的研究热点。本研究利用T2DM小鼠模型对党参大孔树脂60%乙醇分离组份（60%-DS）进行了体内降血糖作用效果研究。采用链脲佐菌素（STZ）结合高脂饲料诱导的T2DM小鼠模型，评价60%-DS对小鼠葡萄糖耐量、胰岛素耐量、血脂四项等生理生化指标的影响。结果证实60%-DS能够降低STZ小鼠的血糖，改善葡萄糖耐量，并在一定程度上改善胰岛素抵抗，提高机体胰岛素的敏感性。 相似文献
6.
7.
目的:观察大明胶囊对2型糖尿病大鼠心功能的作用,并探讨其作用机制。方法:用高脂饮食辅以链脲佐菌素(STZ)建立2型糖尿病大鼠模型,将空腹血糖值大于16.7mmol/L的大鼠随机分6组:糖尿病模型组、大明胶囊大剂量(200mg/kg/d)组、中剂量(100mg/kg/d)组、小剂量(50mg/kg/d)组、对照药罗格列酮(0.8mg/kg/d)组。各给药组连续灌胃给药30天后,HE染色和电镜观察大鼠心肌病理形态学改变;RT-PCR方法测定L型Ca2+通道α1cmRNA和K+通道KV4.2mRNA的表达。结果:大明胶囊可改善2型糖尿病所引起的LVSP、+dp/dtmax下降(P0.05),降低LVEDP、-dp/dt-max水平(P0.05),以中剂量作用最明显;形态学显示大明胶囊中剂量减轻了糖尿病大鼠心肌损伤;中剂量还可缩短注射SNP和PE后血压恢复时间以及增加NTS区c-fos免疫反应阳性细胞个数。结论:a.大明胶囊通过影响KV4.2和α1cmRNA的表达改善Q-T间期和P-R间期的延长;b.大明胶囊可通过改善糖尿病大鼠心肌结构的重塑提高2型糖尿病大鼠心脏收缩和舒张功能。 相似文献
8.
9.
四氧嘧啶致小鼠、大鼠糖尿病模型研究 总被引：1，自引：0，他引：1
目的:研究四氧嘧啶尾静脉注射小鼠和大鼠诱导糖尿病模型,观察不同剂量四氧嘧啶诱导糖尿病模型的稳定性.方法1分别对小鼠尾静脉注射四氧嘧啶60、70、80mg/kg和对大鼠尾静脉注射四氧嘧啶30、40、50mg/kg,测定不同时点小鼠和大鼠的血糖值及体重.结果60、70和80mg/kg四氧嘧啶剂量均可导致小鼠糖尿病模型,但70和80mg/kg剂量组小鼠死亡率较大,而60mg/kg组小鼠糖尿病模型稳定;30mg/kg四氧嘧啶剂量不能造成大鼠糖尿病模型,40和50mg/kg四氧嘧啶剂量均可导致大鼠糖尿病模型,但四氧嘧啶50mg/kg剂量组大鼠死亡率较大,只有40mg/kg组糖尿病大鼠模型稳定.结论:造成小鼠和大鼠糖尿病模型最佳剂量分别为60mg/kg和40mg/kg. 相似文献
10.
本文介绍了利用离子交换法处理含铬废水回收铬（Ⅵ）,制备重铬酸钾的方法。该法不仅处理量大,而且处理效果好,是一种既经济又高效、快速处理电镀等含铬废水的方法。 相似文献
11.
J. O. Olanlokun 《Indian journal of clinical biochemistry : IJCB》2008,23(1):62-66
The effect of oral administration of vitamin E for twenty-eight consecutive days on blood glucose, reduced glutathione levels,
antioxidant enzymes (superoxide dismutase and catalase activities), and levels of malondialdehyde (as an index of free radical-mediated
lipid peroxidation) was observed in the whole blood and liver of normal and alloxan-induced diabetic rats. It was found that
oral administration of vitamin E significantly (p<0.05) lowered the blood glucose level and increased the body weight of the
diabetic rats. The activities of superoxide dismutase and levels of reduced glutathione increased significantly (p<0.05) while
the level of lipid peroxidation decreased. 相似文献
12.
Amar B. Singh J. P. Chaturvedi T. Narender Arvind K. Srivastava 《Indian journal of clinical biochemistry : IJCB》2008,23(4):391-393
Peganum harmala L. (Zygophyllaceae) is a traditional medicine used for the treatment of variety of human ailments, including
antidepression, hallucination, antileishmaniasis etc. We report for first time the hypoglycemic activity of the ethanolic
extract of this plant at two dose levels of 150 and 250mg/kg bw in sucrose challenged normal as well as in rats with streptozotocin
induced diabetes. The oral administration of ethanolic extract causes maximum fall of blood glucose level to 22.9% (p<0.05)
and 29.4% (p<0.01) respectively with the two doses in normal and 30.3% (p<0.01) and 48.4% (p<0.001) in diabetic rats. The
standard drug metformin treated group showed 28.0% (p<0.01) and 45.5% (p<0.001) respectively in normal and diabetic rats.
The above results show that the ethanolic extract of P. harmala is as effective as metformin in reducing the blood glucose
levels of normoglycemic and streptozotocin-induced diabetic rats. 相似文献
13.
P. Pavana S. Sethupathy S. Manoharan 《Indian journal of clinical biochemistry : IJCB》2007,22(1):77-83
The aim of the present study was to evaluate the antihyperglycemic and antilipidperoxidative effects of ethanolic seed extract
ofTephrosia purpurea (TpEt) in streptozotocin induced diabetic rats. Hyperglycemia associated with an altered hexokinase and glucose 6 phosphatase
activities, elevated lipid peroxidation, disturbed enzymatic and non-enzymatic antioxidants status were observed in streptozotocin
induced diabetic rats. Oral administration of “TpEt” at a dose of 300mg/kg bw showed significant antihyperglcemic and antilipidperoxidative
effects as well as increased the activities of enzymatic antioxidants and levels of non enzymatic antioxidants. We also noticed
that the antihyperglycemic effect of plant drug (TpEt) was comparable to that of the reference drug glibenclamide. Our results
clearly indicate that “TpEt” has potent antihyperglycemic and antilipidperoxidative effects in streptozotocin induced diabetic
rats and therefore further studies are warranted to isolate and characterize the bioactive antidiabetic principles from “TpEt”. 相似文献
14.
P. K. Rai D. Jaiswal S. Mehta D. K. Rai B. Sharma Geeta Watal 《Indian journal of clinical biochemistry : IJCB》2010,25(2):175-181
This study deals with the effects of freeze dried rhizome powder of Curcuma longa (C. longa) dissolved in milk on normal as
well as diabetic models. Diabetes of type II and type I was within 3 days of a single administration of doses of 45 and 65
mg kg−1 of streptozotocin respectively. Various parameters such as blood glucose levels, triglycerides, total cholesterol, high density
lipoprotein, very low density lipoprotein, low density lipoprotein, serum glutamic oxaloacetic transaminase, serum glutamic
pyruvate transaminase, alkaline phosphatase, creatinine, hemoglobin, urine protein and urine sugar in addition to body weight
were taken in to consideration and were analyzed after administration of variable doses of rhizome powder. The dose of 200
mg kg−1 was identified as the most effective dose as it increased HDL, Hb and bw (P<0.05) with significant decrease in the levels
of blood glucose, lipid profile and hepatoprotective enzymes (P<0.001). 相似文献
15.
M. Bokaeian A. Nakhaee Bita Moodi A. Farhangi Azim Akbarzadeh 《Indian journal of clinical biochemistry : IJCB》2010,25(2):182-187
The anti-candidial effect of garlic extract (Allium sativum L.) was investigated in normal and streptozotocin-induced diabetic
rats. Diabetes was induced after a single intraperitoneal injection of streptozotocin (60 mg/kg). Rats were divided into six
groups with fifteen rats in each group: (1) Normal control rats (2) Control rats + C. albicans (3) Control rats + garlic extract
+ C. albicans (4) Diabetic control rats (5) Diabetic rats + C. albicans (6) Diabetic rats + garlic extract + C. albicans.
The concerned groups were inoculated with C.albicans on the 15 th day. At the end of one month experiment, fasted rats were
killed by cervical decapitation. Blood was collected for estimation of glucose and C. albicans concentrations were estimated
in liver and kidneys homogenates. A significant increase was observed in serum glucose levels in diabetic rats. A loss of
bodyweight, polydipsia and polyphagia were observed in diabetic rats. Administration of alcoholic extract of garlic (0.25
g/kg body weight) reduced the hyperglycemia, polydipsia, polyphagia and associated weight loss of streptozotocin-treated rats.
Administration of garlic extract significantly reduced C. albicans concentrations in liver and kidneys homogenates in infected
control and diabetic rats. It is concluded that garlic extract improves candidia infection in diabetic rats. 相似文献
16.
Mahalingam Gayathri Krishnan Kannabiran 《Indian journal of clinical biochemistry : IJCB》2008,23(4):394-400
The aim of the present study was to evaluate the antidiabetic and ameliorative potential of aqueous extract of Ficus bengalensis
bark in streptozotocin induced diabetic rats. The effect of oral administration of aqueous extract of F. bengalensis bark
on blood glucose, serum electrolytes, serum glycolytic enzymes, liver microsomal protein, hepatic cytochrome P-450 dependent
monooxygenase enzymes and lipid peroxidation in liver and kidney of streptozotocin -induced diabetic rats was studied. Oral
administration of Ficus bengalensis to fed, fasted and glucose loaded diabetic rats significantly [F > 0.05 (ANOVA) and P<
0.05 (DMRT)] decreased the blood glucose level at 5 hrs and restored the levels of serum electrolytes, glycolytic enzymes
and hepatic cytochrome P-450 dependent enzyme systems and decreased the formation of liver and kidney lipid peroxides at the
end of 12 weeks. Further, the aqueous extract of Ficus bengalensis at a dose of 500mg/kg/day exhibits significant antidiabetic
and ameliorative activity as evidenced by histological studies in normal and Ficus bengalensis treated streptozotocin induced
diabetic rats. On the basis of our findings, it could be used as an antidiabetic and ameliorative agent for better management
of diabetes mellitus. 相似文献
17.
The hyperlipidemia, fatty liver and the high levels of liver and kidney thiobarbituric acid reactive substances (TBARS) observed
in rats which were fed ethanol for 45 days, could be significantly reduced by feeding diacetodibutyl disulphide (DADBDS).
Ethanol-induced hypoproteinemia and the rise in serum enzymes like AST (EC 2.6.1.10), ALT (EC 2.6.1.2) and ALP (EC 3.1.3.1)
could also be ameliorated by DADBDS. Feeding of this compound to normal rats did not produce any change in serum or tissue
lipid levels or serum enzymes or tissue TBARS except a moderate reduction in serum triacyl glycerols. DADBDS feeding to rats
maintained on a high lipid diet could also reduce the serum and tissue lipid levels and also reduce the serum transaminases.
DADBDS which is an aliphatic disulphide could produce hypolipidemic effects in rats fed a single large dose of ethanol, whereas
dimenthol disulphide which is an aromatic disulphide was not useful as a hypolipidemic agent. Perhaps hypolipidemic effects
are shown only by aliphatic disulphides and not by aromatic disulphides. Feeding of 100 mg DADBDS per kg body weight to normal
fasted rats produced a mild hypoglycemia, but higher doses produced a hyperglycemic effect. This dose of DADBDS increased
the serum insulin levels and reduced blood glucose levels in fasted diabetic rats, but DADBDS feeding did not alter the serum
insulin levels in fasted normal rats. DADBDS is odourless and tasteless in 1% solution and it could be a better substitute
for garlic for hypoglycemic and hypolipidemic studies. 相似文献
18.
Induction of diabetes by Streptozotocin in rats 总被引：1，自引：0，他引：1
A. Akbarzadeh D. Norouzian M. R. Mehrabi Sh. Jamshidi A. Farhangi A. Allah Verdi S. M. A. Mofidian B. Lame Rad 《Indian journal of clinical biochemistry : IJCB》2007,22(2):60-64
The objective of this study is to induce experimental diabetes mellitus by Streptozotocin in normal adult Wistar rats via
comparison of changes in body weight, consumption of food and water, volume of urine and levels of glucose, insulin and C-peptide
in serum, between normal and diabetic rats. Intra-venous injection of 60mg/kg dose of Streptozotocin in adult wistar rats,
makes pancreas swell and at last causes degeneration in Langerhans islet beta cells and induces experimental diabetes mellitus
in the 2–4 days. Induction of experimental diabetes mellitus is indeed the first step in the plan of purification of pancreatic
Langerhans islet cells of normal rats for transplanting under the testis subcutaneous of experimentally induced diabetic rats.
Streptozotocin induces one type of diabetes which is similar to diabetes mellitus with non-ketosis hyperglycemia in some animal
species. For induction of experimental diabetes in male adult rats weighted 250–300 grams (75–90 days), 60mg/kg of Streptozotocin
was injected intravenously. Three days after degeneration of beta cells, diabetes was induced in all animals. The diabetic
and normal animals were kept in the metabolic cages separately and their body weight, consumption of food and water, urine
volume, the levels of serum glucose, insulin and C-peptide quantities in all animals were measured and then these quantities
were compared. For a microscopic study of degeneration of Langerhans islet beta cells of diabetic rats, sampling from pancreas
tissue of diabetic and normal rats, staining and comparison between them, were done. Induction of diabetes with Streptozotocin
decreases Nicotinamide-adenine dinucleotide (NAD) in pancreas islet beta cells and causes histopathological effects in beta
cells which probably intermediates induction of diabetes. In this study, we used Streptozotocin for our experiments in induction
of experimental diabetes mellitus. After Induction of diabetes, consumption of food and water, volume of urine and glucose
increased in the diabetic animals in comparison with normal animals, but the weight of body and the volume of insulin and
C-peptide decreased in the diabetic animals. Sampling and staining of pancreas tissue of diabetic and normal rats showed that
the Langerhans islet beta cells of diabetic rats have been clearly degenerated. In three days, Streptozotocin makes pancreas
swell and at last causes degeneration in Langerhans islet beta cells and induces experimental diabetes. It also changes normal
metabolism in diabetic rats in comparison with normal rats. Consumption of water and food, volume of urine, serum glucose
increases in diabetic animals in comparison with normal rats but the levels of serum insulin, C-peptide and body weight decreases. 相似文献
19.
N. P. Suryawanshi A. K. Bhutey A. N. Nagdeote A. A. Jadhav G. S. Manoorkar 《Indian journal of clinical biochemistry : IJCB》2006,21(1):126-130
The study was designed to find out the correlation between lipid peroxidation, lipoprotein levels to severity and complication
of diabetes mellitus. Degree of lipid peroxidation was measured in terms of malondialdehyde (MDA) along with lipid profile
and blood glucose in diabetes mellitus. It is categorised into insulin dependent diabetes mellitus (IDDM), non insulin dependent
diabetes mellitus (NIDDM) and diabetes mellitus(DM) with complication.
Total 112 known diabetic patients and 52 non-diabetic controls were studied. These patients were grouped as per the concentration
of fasting blood glucose level i.e. controlled, poorly controlled, and uncontrolled group. There are significant increase
in the lipid peroxide (MDA) and lipid profile except HDL cholesterol which is decreased, has been found in all groups as compared
to controls In NIDDM group lipid peroxidation was markedly increased than IDDM group and it was higher in DM with complications.
Other finding observed was that the level of lipid peroxide increased as per the increase in concentration of blood glucose.
The increase lipid peroxidation in the hyperglycemic condition may be explained, as the superoxide dismutase enzyme which
is antioxidant becomes inactive due the formation of superoxide radical within the cell. Maximum lipid peroxidation leads
to the damage of the tissue and organs which results into complication in diabetic patients. High levels of total cholesterol
appear due to increased cholesterol synthesis. The triglyceride levels changes according to the glycemic, control. The increase
may be due to overproduction of VLDL-TG.
It is concluded that good metabolic control of hyperglycemia will prevent in alteration in peroxidation and the lipid metabolism,
which may help in good prognosis and preventing manifestation of vascular and secondary complication in diabetes mellitus. 相似文献
20.
Mohamed Ahmed Abdelmoaty M. A. Ibrahim N. S. Ahmed M. A. Abdelaziz 《Indian journal of clinical biochemistry : IJCB》2010,25(2):188-192
Quercetin (QE), one of natural flavanoid group, was widely distributed as a secondary metabolite in plant kingdom. It has
been believed that oxidative stress plays a role in the pathogenesis of diabetes mellitus (DM). The aim of the present study
was the evaluation of possible effects of QE on blood glucose and antioxidant enzymes in experimental streptozotocin (STZ)-induced
diabetes in rats. STZ was injected intraperitoneally with single dose of 50 mg/kg for diabetes induction. QE (15 mg/kg bw
day, intraperitoneal (i.p.) injection) was injected for 3 days prior to STZ administration; these injections were continued
to the end of the study (for 25 days). Glucose tolerance test and random plasma glucose were done for all animals. Cellular
antioxidant enzymes such as glutathione peroxidase (GSHPx), superoxide dismutase (SOD) and catalase (CAT) activities were
measured in pancreatic homogenates. Quercetin had no effect on plasma glucose level of normal animals but its pre- treatment
was able to prevent diabetes induced by single intraperitoneal injection of streptozocintreated rats. Antioxidant enzyme activity
significantly decreased in STZ induced diabetic group. QE treatment significantly increased the antioxidant enzyme activities.
It could be concluded that quercetin, a flavonoid with antioxidant properties, exerting its beneficial antidiabetic effects. 相似文献