共查询到15条相似文献，搜索用时 171 毫秒
1.
通过单因子试验和多因子正交试验,优化筛选了适于绣球菌摇瓶培养条件。结果表明,适于产菌丝体的摇瓶条件为:培养温度28℃,培养基起始pH4.5,接种量8%,摇瓶转速100r/min;摇瓶培养适于产菌丝体的最适碳氮源及其浓度分别为:可溶性淀粉2.5%,蛋白胨0.15%。适于产胞外多糖的摇瓶条件为:培养温度29℃,培养基起始pH5.5,接种量8%,摇瓶转速100r/min;摇瓶培养最适碳氮源及其浓度分别为:可溶性淀粉2.5%,蛋白胨0.25%。 相似文献
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以紫外分光光度法作为庆大霉素含量测定手段,采用正交试验方法,对紫色小单孢菌产庆大霉素的发酵培养基及发酵条件进行优化,通过统计学分析,确定发酵培养基最优组合为:黄豆饼粉3.5%,CaCO30.5%,淀粉5.5%,CoCl20.0006%。优化的发酵条件为:种龄60 h,接种量12 mL,装液量50 mL/500 mL摇瓶。在此优化条件下添加一定量的氨基酸培养6 d,庆大霉素效价较优化前提高了85.5%。用紫外分光光度法与微生物法测定庆大霉素发酵液效价产生的误差小于4%,该方法可以用于教学实验中庆大霉素液体发酵培养基优化的快速筛选。 相似文献
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通过单因子试验和多因子正交试验,优化筛选了适于虎掌菌（Tremellodon gelatinosum）摇瓶培养条件．结果表明：虎掌菌摇瓶培养的最佳条件是2．5％可溶性淀粉,0．25％的牛肉膏,摇瓶转速120r·min^-1．温度25℃,接种量为10％（V／V）,最佳发酵时间是6d． 相似文献
4.
摇瓶中对益生素生产菌———产乳酸芽孢杆菌JY-LZ培养条件进行了研究,在发酵培养基培养时,得到最适培养条件为接种量5%~10%,温度30~40℃,灭菌前pH值7~9,100 mL摇瓶装液量30 mL,48 h培养密度大于1010/mL,芽孢率大于50%,可以进一步开发应用于生产实际. 相似文献
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强聚硒酵母摇瓶发酵条件的优化研究 总被引：1，自引：1，他引：0
罗世炜 《襄樊职业技术学院学报》2009,8(2)
通过摇瓶培养条件试验确定了强聚硒酵母最适发酵条件:麦芽汁%,初始硒浓度20μg/mL,在其发酵进入到对数生长期后再添加亚硒酸钠使硒浓度达到60μg/mL,接种量为10%,在28℃、200 r/min的摇床上培养29 h.在此条件下,强聚硒酵母生物量(干重)达到8.25 g/L,酵母细胞中硒含量达到1230.21μg/g. 相似文献
7.
细黄链霉菌产木聚糖酶的研究 总被引：1，自引：0，他引：1
通过多因子摇瓶发酵优化实验,以细黄链霉菌为始菌,研究产木聚糖酶的情况.以甘蔗渣为惟一碳源,测定不同甘蔗渣含量的培养基中细黄链霉菌所产木聚糖酶的活力,同时测定氮源、接种量对该菌产木聚糖酶的影响,结果表明,其最适碳源含量为10%,最适氮源为酵母膏和硫酸铵的混合氮源,其最适接种量为5%;在装料50mL的三角摇瓶中于30℃、120rpm振荡培养5d后,发酵液中木聚糖酶活力达7.74IU/mL. 相似文献
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对14份土样和7株实验室保藏菌株经初筛、摇瓶复筛,得到1株油脂产量较高的酵母菌,其油脂含量为7.34%。对影响该菌体产油脂的条件进行了单因素和正交实验,得出摇瓶发酵培养的最佳产油脂条件:发酵周期为4d,酵母膏和硫酸铵混合物为氮源,接种量为12%。在该条件下测得的菌体生物量为12.211g/L,油脂产量为1.524g/L,油脂含量为12.48%。 相似文献
9.
本文首先从几种常用的细菌培养基中筛选适合工程菌DH5α/pDH表达的重组人粒细胞-巨噬细胞集落刺激因子（rhGM-CSF）的培养基,其次,对摇瓶培养条件进行了初步优化,着重研究了培养温度、诱导时机,诱导维持时间,接种量对E.coli生长和rhGM-CSF表达的影响。在优化条件下,rhGM-CSF的表达量为26.3%。 相似文献
10.
以柑桔和胡萝卜为主要原料,分别进行酒精发酵、醋酸液体摇瓶发酵、下胶澄清等方法制成柑桔胡萝卜保健果醋.试验结果表明:酒精发酵的适宜条件为:活性干酵母接种量1‰,发酵温度32℃,发酵时间24h;醋酸发酵的适宜条件为:醋酸菌接种量10%,发酵温度33℃,发酵时间48h;果醋下胶澄清:采用1%壳聚糖的澄清效果最佳. 相似文献
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The optimization of cultural conditions for β-glucanase production byBacillus subtilis ZJF-1A5 was investigated in flask trials. Temperature had great effect on β-glucanase production which maximized at optimal
temperature of 37°C and decreased significantly when temperature was over 37°C. Charge quantity affected β-glucanase production
significantly. Adding oxygen vector N-dodecane or acetic ether benefited β-glucanase production, but it depended on the concentration
and charge quantity. The results of fractional factorial design showed that age and size of inoculum and shaking speed were
the key factors affecting β-glucanase production and the cultivation time span to reach the highest β-glucanase activity.
The optimal cultural conditions for β-glucanase production obtained with CCD were as follows: inoculum age and size (16 h,
3.82% (v/v)), shaking speed 210 r/min, charge quantity of 30 mL in 250 mL flask and initial pH 7.0, cultured at 37°C for 50
h. Repeated experimental results accorded with those predicted by a second-order polynomial model. The amount of β-glucanase,
α-amylase and neutral protease produced byB subtilis ZJF-1A5 was associated partially with cell growth. Those three enzymes' activities increased following the cell growth and
increased significantly when cells entered the stationary phase.
Project (No. B0608) supported by the National Natural Science Foundation of China and a grant (2001121B25) from Hangzhou Science
and Technology Development Project of Zhejiang Province, China 相似文献
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Sequential methodology based on the application of three types of experimental designs was used to optimize the fermentation conditions for elastase production from mutant strain ZJUEL31410 of Bacillus licheniformis in shaking flask cul- tures. The optimal cultivation conditions stimulating the maximal elastase production consist of 220 r/min shaking speed, 25 h fermentation time, 5% (v/v) inoculums volume, 25 ml medium volume in 250 ml Erlenmeyer flask and 18 h seed age. Under the optimized conditions, the predicted maximal elastase activity was 495 U/ml. The application of response surface methodology resulted in a significant enhancement in elastase production. The effects of other factors such as elastin and the growth factor (corn steep flour) on elastase production and cell growth were also investigated in the current study. The elastin had no significant effect on enzyme-improved production. It is still not clear whether the elastin plays a role as a nitrogen source or not. Corn steep flour was verified to be the best and required factor for elastase production and cell growth by Bacillus licheniformis ZJUEL31410. 相似文献
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为提高棉花黄萎病拮抗细菌HMB-1005的芽孢形成率及芽孢数量,分析摇瓶发酵对芽孢形成的主要影响因素．通过单因素试验和正交试验对HMB-1005菌株产芽孢条件进行分析,确定最佳摇瓶发酵条件为：蔗糖1％、玉米粉2％、CaCl2·2H2O0．02％和NaH2PO4·2H2O0．2％、pH值为7．0、温度为30℃、转速为200r／min、接种量10％、种龄24h。在此优化条件下,发酵液中拮抗菌出芽率达到90％以上． 相似文献
14.
以饲料为原料筛选出一株既产乳酸又产芽孢的益生菌,经过分子生物学鉴定为凝结芽孢杆菌(Bacillus coagulans),命名为T-8。从培养基碳源、氮源、有机物浸出液、培养基pH、培养温度、培养时间等方面对菌株T-8展开发酵特性研究,结果表明,最优培养基配方为:玉米粉6 g/L、豆粕粉15 g/L、小麦麸皮浸出液60%、酵母粉5 g/L、蛋白胨10 g/L、氯化钠10 g/L,pH 5.0。以5%的接种量将种子液转接至最优培养基,在47℃的摇床中以200 r/min的转速振荡培养28 h,菌株T-8的细胞密度达到4.8×109 CFU/mL,芽孢率达到95.2%。本试验可为益生菌凝结芽孢杆菌应用于饲用微生物菌剂的工业化生产提供参考。 相似文献