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目的：研究黑乳海参（Holothuria nobilis Selenka）中硫酸化多糖类型、理化性质及抗凝活性。方法：利用化学和物理相结合的分离方法进行纯化,采用色谱、化学、光谱等方法对其纯度、化学组成、分子量、特性黏度、旋光度、凝血活性等进行分析。结果：分离获得F—I、F—II两种多糖组分。F—I由葡萄糖醛酸、乙酰氨基半乳糖、岩藻糖和硫酸基（摩尔比1．00：0．96：1．18：2．99）组成;F-II仅由岩藻糖和硫酸基（摩尔比1．00：0．51）;它们的理化性质显著不同,前者的抗凝效价约为后者的6倍。结论：黑乳海参中至少存在两种不同的硫酸化多糖,岩藻糖化糖胺聚糖和α-L-硫酸化岩藻聚糖,均具有强的抗凝血活性。 相似文献
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田光辉 《陕西理工学院学报(社会科学版)》2004,22(3):67-69,77
将茶树枝粉末脱脂后用水浸提多糖并纯化，分离的多糖具有降血糖的作用，对此多糖进行了理化性质分析，并利用纸层析和气相色谱仪对多糖中的单糖组成进行分析，组成分析表明此多糖的单糖组成为萄葡糖、半乳糖、甘露、阿拉伯糖，有少量的岩藻糖和核糖． 相似文献
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目的：了解道教学员的人格特征,及其生活满意度状况,通过对比为现代人生活提供借鉴．方法：采用16PF人格问卷和生活满意度量表（SWLS）对湖北武当山道教学院33名学员进行调查．结果：道教学员在16PF的9个维度均与大学生样本存在显著差异,具体表现为B（t=3．92 p〈0．00）、C（t=2．76 p〈0．01）、F（t=-3．19 p〈0．00）、G（t=2．67 p〈0．05）、H（t=2．80 p〈0．00）、L（t=-3．86 p〈0．00）、Q1（t=-4．58 p〈0．00）、Q2（t=-2．91 p〈0．00）、Q3（t=4．07 p〈0．00）;同时道教学员的生活满意度得分显著高于大学生样本（t=2．95,P〈0．01）;道教学员的积极情感显著高于大学生群体（t=3．55,P〈0．01）,其消极情感与大学生群体无显著差异．结论：道教学员人格特征在多个维度与一般大学生存在显著差异,他们生活满意度水平和积极情绪均显著高于后者。 相似文献
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多糖类生物活性研究简介 总被引：1，自引：0，他引：1
多糖类化合物广泛存在于高等植物、微生物、地衣、海藻和动物体内。由于组成多糖的单糖品种繁多(目前已知的单糖有 2 0 0多种 ) ,而且单糖可在多个羟基上以两种可能的键型相互连接 (氨基酸和核苷酸只有一种连接方式 ) ,因此它能以最小的结构单元承载极大的生物信息量。近几十年的研究表明 ,多糖类不仅是细胞的结构物质和能源物质 ,更是具有多种生理功能的生物活性物质 ,在生命现象中参与了细胞的各种活动。1 多糖的免疫调节作用目前普遍认为多糖是一种无细胞毒的免疫促进剂。多糖能在多条途径、多个层面对免疫系统发挥调节作用。多糖不仅能… 相似文献
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绿色植物光合作用的主要产物是什么？实验证明，绿色植物光合作用，是在叶片上叶绿体借光能使周围二氧化碳和水转变成贮有能量的有机物（如淀粉等）并释放出氧气（图1）。研究表明：光合作用形成的有机物有多种，如葡萄糖（单糖）、蔗糖（二糖）、由单糖聚合成的多糖如淀粉、纤维素等。 相似文献
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研究金钱草对草酸钙结石模型小鼠的红、白细胞数目的影响．方法：30只小鼠按体重相近原则分为3组。Ⅰ组作为对照组小鼠自由饮用自来水,Ⅱ、Ⅲ组作为试验组小鼠自由饮用主要由1％氯化铵和1％乙二醇配制成的成石液,每组小鼠每日灌服药液0．5mL,Ⅰ、Ⅱ组小鼠灌服生理盐水,Ⅲ组小鼠灌服金钱草水提取液．28天灌服结束后,选取每组小鼠,乙醚吸入麻醉,立即眶后静脉采血,血样放入加了抗凝剂的样品管．采集的血样用于红细胞计数、白细胞计数和白细胞分类计数．结果：与试验Ⅲ组小鼠白细胞数目和嗜中性粒细胞所占比例两项指标相比,对照组Ⅰ组白细胞数目少了3211个／μL（P〈0．01）,嗜中性粒细胞所占比例增多11％（P〈0．05）;试验Ⅱ组白细胞数目少了2619个／μL（P〈0．01）,嗜中性粒细胞所占比例增多17％（P〈0．05）．结论：金钱草能够通过调整小鼠内白细胞的变化来改变机体的免疫力,进而发挥抗结石的作用． 相似文献
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吕敬松 《毕节师范高等专科学校学报》2012,(8):72-75
以季戊四醇为原料,经多步合成季戊四胺,然后与2-噻吩醛反应,合成了（N1,N2）,（N2,N3）,（N1,N3）-三（噻吩亚甲基）-N-（噻吩亚甲基）-季戊四胺。结构经核磁和X-单晶衍射实验证实。结果表明：其晶体结构属六方晶系,空间群P6（5）,Z=4,晶胞参数：a=1,8385（6）nm,b=1．8385（6）nm,c=1．3866（8）nm,a=90．00,B=90．00,1=120．00,V=4．05873nm3,F（000）=1740．0,Mu=0．39mm-1,R1=0．0719．Rw=0．214． 相似文献
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吴春美 《内江师范学院学报》2012,27(8):52-54
介绍了红薯茎叶多糖的提取、分离、纯化方面的技术进展,阐述了目前对红薯茎叶多糖的结构分析和生物活性研究的现状,讨论了当前研究存在的问题及今后的发展趋势．目前的研究结果表明：红薯叶多糖是由木糖、甘露糖、葡萄糖构成;红薯茎叶多糖具有降血糖功能、抗氧化活性、抑菌作用;红薯叶多糖的最佳提取工艺为：提取时间12h、温度100℃、料水比1：110,提取次数3次,在此工艺下多糖得率为6．5％;正丁醇一三氯乙酸（20：1）法是红薯茎叶粗多糖除蛋白的最好方法．但对红薯茎叶多糖的活性部位、多糖组分的研究还不透彻;在提取工艺方面,超声波和微波辅助提取新型提取技术在提搞红薯茎叶多糖的得率等方面将具有重要的应用前景． 相似文献
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HMS mesoporous molecular sieve was synthesized hydrothermally by using dodecylamine (DDA) as template and tetethylorthosilicalite (TEOS) as silicon source. The influence of the hydrothermal synthesis conditions on HMS particle size was studied systematically. The results showed that the smaller particle was obtained under the condition of DDA/SiO2=0.27, H2O/SiO2=66.7, EtOH/SiO2=6.5 mol and synthesis time of 18 h. The presences of additives (TW20, TW60, SP60 and Neopelex) also helped to the reduction of particle size. The HMS mesoporous molecular sieve with particle size around 60～100 nm was obtained by using TW20 or TW60 as an additive. In addition, TEM image showed that HMS mesoporous molecular sieve with pariticle size about 20～100 nm was synthesized in microemulsion. 相似文献
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Jian Yi Meng Meng Zhong-qiu Liu Jin-fang Zhi Yuan-yang Zhang Jing Xu Ya-bin Wang Jin-ting Liu Ri-mo Xi 《Journal of Zhejiang University. Science. B》2012,13(2):118-125
To detect gatifloxacin (GAT) residue in swine urine, an electrochemical immunoassay was established. An indirect competitive
immunoassay was developed, in which the coating antigen is immobilized in an enzyme-linked immunosorbent assay (ELISA) plate
and GAT residue from the sample competes with the limited binding sites in added anti-GAT antibody. Horseradish peroxidase
(HRP) conjugated to goat anti-rabbit IgG was used as the enzymatic label. A carbon fiber working electrode was constructed
and current signals were detected by using hydrogen peroxide as a substrate and hydroquinone as an electrochemical mediator.
The electrochemical immunoassay was evaluated by analysis of GAT in buffer or swine urine and an average value of half inhibition
concentration (IC50) of 8.9 ng/ml was obtained. Excellent specificity of the antibody was achieved with little cross-reaction with lomefloxacin
(3.0%), ciprofloxacin (3.0%), and ofloxacin (1.9%) among commonly used (fluoro)quinolones. In conclusion, the immunoassay
system developed in this research can be used as a rapid, powerful and on-site analytical tool to detect GAT residue in foods
and food products. 相似文献
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Heparin was grafted onto polycarbonate urethane (PCU) surface via a three-step procedure utilizing α, ωdiamino-poly(ethylene glycol) (APEG, M n =2 000) as a spacer. In the first step, isocyanate functional groups were introduced onto PCU surface by the treatment of hexamethylene diisocyanate (HDI) in the presence of di-n-butyltin dilaurate (DBTDL) as a catalyst. In the second step, APEG was linked to the PCU surface to obtain the APEG conjugated PCU surface (PCU-APEG). In the third step, heparin was covalently coupled with PCU-APEG in the presence of N-hydroxysuccinimide (NHS) and 1-ethyl-3-(3-dimethylamidopropyl) carbodiimide (EDAC). The amount of heparin (1.639 μg/cm 2 ) covalently immobilized on the PCU-APEG surface was determined by the toluidine blue method. The modified surface was characterized by water contact angle, attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR), X-ray photoelectron spectroscopy (XPS), and atomic force microscopy (AFM). The hemocompatibility was preliminarily studied by platelet adhesion test. The results indicated that heparin was successfully grafted onto the PCU surface, and meanwhile the hydrophilicity and hemocompatibility of the modified PCU surface were improved significantly compared with the blank PCU surface. 相似文献
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合成了枝状PBS基脂肪族聚酯——聚丁二酸丁二醇酯-共-聚丁二酸1,2丙二醇酯P(BS-co-1,2-PS),并用土壤培养液法对P(BS-co-1,2-PS)及PBS进行降解,用X射线衍射对材料的结晶度进行了测定,通过失重率的变化和表面形貌观察对降解程度进行表征分析。结果表明,结晶度的变化顺序为PBSP(BS-co-1,2-PS)-10%P(BS-co-1,2-P S)-20%P(BS-co-1,2-P S)-30%,降解性能的变化顺序为P(BS-co-1,2-P S)-30%P(BS-co-1,2-PS)-20%P(BS-co-1,2-PS)-10%PBS,由此得出,随着1,2丙二醇添加量的增大,P(BS-co-1,2-PS)共聚酯主链结构对称性降低,结晶性能减弱,结晶度减小,降解性能增大。 相似文献
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INTRODUCTION Because of its low molecular weight and ability to fluoresce independently (George, 1997), the new molecular tag, green fluorescent protein (GFP), has become more and more popular after Prasher et al.(1992) cloned its cDNA in 1992. There are many reports describing the co-expression of GFP and a specific antibody or cytokine gene, with the fusion protein expressing the fluorescent activity and bio-logical activity of the complement protein (Haraguchi et al., 1999; Mclean… 相似文献
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GM(1,1) and GM(1,1) rolling models derived from grey system theory were estimated using time-series data from projection studies by National Center for Education Statistics (NCES). An out-of-sample forecasting competition between the two grey prediction models and exponential smoothing used by NCES was conducted for education expenditure and school enrollment under the assumption that grey prediction was as promising as NCES's forecasting technique in dealing with univariate time-series data while some other determinants of the variables under examination were excluded. The purpose of this study, therefore, was to verify that the GM(1,1), and GM(1,1) rolling models would provide forecasts that were at least as accurate as the NCES's approach to extrapolating education expenditure and school enrollment. The findings revealed that the forecasting efficiency of GM(1,1) rolling model was superior to exponential smoothing and GM(1,1) model. The results can offer valuable insights and provide a basis for further research in model building for short-term estimation on educational statistics. 相似文献
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Objective: To observe the effects of fructose-1,6-diphosphate (FDP) on serum levels of cardiac troponin 1 (cTnl) and creatine kinase-MB (CK-MB), as well as the concentration of calcium in cardiomyocytes (Myo[Ca2 ]) and activity of sarcoplosnic Ca2 -ATPase (SRCa2 -ATPase) in Adriamycin (ADR)-treated rats. Methods: Rats were intraperitoneally injected with ADR (2.5 mg/kg every other day for 6 times) and then with different dosages of FDP (every other day for twenty-one times). Bi-antibodies sandwich Enzyme linked immune absorption assay (ELISA) was performed to detect serum level of cTnl. CK-MB was detected by monoclonal antibody, Myo[Ca2 ] was detected by fluorescent spectrophotometry and the activity of SRCa2 -ATPase was detected by inorganic phosphate method. Results: FDP (300, 600, 1200 mg/kg) significantly reduced the serum levels of cTnl and CK-MB, while at the same time decreased calcium concentration and increased SRCa2 -ATPase activity in cardiomyocytes of ADR-treated rats (P<0.01). Conclusions: FDP might alleviate the cardiotoxic effects induced by ADR through decreasing calcium level as well as increasing SRCa2 -ATPase activity in cardiomyocytes. 相似文献
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采用化学气相沉积法以脱油沥青为碳源制备出碳微球,运用硬脂酸对碳微球进行表面修饰,并研究了硬脂酸修饰后碳微球作为润滑油添加剂的分散性和稳定性.利用场发射扫描电镜、高分辨透射电镜分析了碳微球的晶体结构和形貌特征,结果表明所制备的碳微球纯度很高、粒径均匀(500 nm).傅立叶红外实验证明,通过酯化反应碳微球能够被硬脂酸包覆,显著提高了碳微球在润滑油中的分散性和稳定性. 相似文献