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1.
Although the biochemical dissection of parasitoid-host interactions is becoming well characterized, the molecular knowledge concerning them is minimal. In order to understand the molecular bases of the host immune response to parasitoid attack, we explored the response of Papilio xuthus parasitized by the endoparasitic wasp Pteromalus puparum using proteomic approach. By examining the differential expression of plasma proteins in the parasitized and unparasitized host pupae by two-dimensional (2D) electrophoresis, 16 proteins were found to vary in relation to parasitization compared with unparasitized control samples. All of them were submitted to identification by mass spectrometry coupled with a database search. The modulated proteins were found to fall into the following functional groups: humoral or cellular immunity, detoxification, energy metabolism,and others. This study contributes insights into the molecular mechanism of the relationships between parasitoids and their host insects. 相似文献
2.
3.
This paper proposes a new approach for multi-objective robust control. The approach extends the standard generalizedl
2 (Gl
2) and generalizedH
2 (GH
2) conditions to a set of new linear matrix inequality (LMI) constraints based on a new stability condition. A technique for
variable parameterization is introduced to the multi-objective control problem to preserve the linearity of the synthesis
variables. Consequently, the multi-channel multi-objective mixed Gl
2/GH
2 control problem can be solved less conservatively using computationally tractable algorithms developed in the paper.
Project supported by the National Natural Science Foundation of China (No. 60374028), and the Scientific Research Foundation
for Returned Overseas Chinese Scholars, Ministry of Education (No. [2004]176) 相似文献
4.
α-diketones and β-diketones react with Grignard reagents in the presence of a catalytic amount of Cp2TiCl2 to yield α-ketols and corresponding ketones respectively. Sulfoxides can be deoxygenated by Cp2TiCl2/Al system. The possible mechanisms are also discussed.
Project (No. 20072033) supported by the National Natural Science Foundation of China and the Specialized Research Fund for
the Doctoral Program of Higher Education Ministry, China 相似文献
5.
Magnaporthe oryzae has been used as a primary model organism for investigating fungus-plant interaction. Many researches focused on molecular
mechanisms of appressorium formation to restrain this fungal pathogen. Autophagy is a very high conserved process in eukaryotic
cells. Recently, autophagy has been considered as a key process in development and differentiation in M. oryzae. In this report, we present and discuss the current state of our knowledge on gene expression in appressorium formation and
the progress in autophagy of rice blast fungi.
Project supported by the National Natural Science Foundation of China (Nos. 30671351 and 30870101) and the Hi-Tech Research
and Development Program (863) of China (No. 2002AA245041) 相似文献
6.
Scrubbing of NO
x
from the gas phase with Fe(II)EDTA has been shown to be highly effective. A new biological method can be used to convert
NO to N2 and regenerate the chelating agent Fe(II)EDTA for continuous NO absorption. The core of this biological regeneration is how
to effectively simultaneous reduce Fe(III)EDTA and Fe(II)EDTA-NO, two mainly products in the ferrous chelate absorption solution.
The biological reduction rate of Fe(III)EDTA plays a main role for the NO
x
removal efficiency. In this paper, a bacterial strain identified asKlebsiella Trevisan sp. was used to demonstrate an inhibition of Fe(III)EDTA reduction in the presence of Fe(II)EDTA-NO. The competitive inhibition
experiments indicted that Fe(II)EDTA-NO inhibited not only the growth rate of the iron-reduction bacterial strain but also
the Fe(III)EDTA reduction rate. Cell growth rate and Fe(III)EDTA reduction rate decreased with increasing Fe(II)EDTA-NO concentration
in the solution.
Project (No. 20176052) supported by the National Natural Science Foundation of China and the Scientific Research Foundation
for Returned Overseas Chinese Scholars, Ministry of Education 相似文献
7.
Recently there has been an increasing interest in applying random walk based methods to recommender systems. We employ a Gaussian random field to model the top-N recommendation task as a semi-supervised learning problem, taking into account the degree of each node on the user-item bipartite graph, and induce an effective absorbing random walk （ARW） algorithm for the top-N recommendation task. Our random walk approach directly generates the top-N recommendations for individuals, rather than predicting the ratings of the recommendations. Experimental results on the two real data sets show that our random walk algorithm significantly outperforms the state-of-the-art random walk based personalized ranking algorithm as well as the popular item-based collaborative filtering method. 相似文献
8.
Breast cancer is one of the leading causes of death in women today. Some of the patients are hereditary, with a large proportion
characterized by mutation in BRCA1 and/or BRCA2 genes. In this review, we provide an overview of these two genes, focusing on their relationship with hereditary breast cancers.
BRCA1/2 associated hereditary breast cancers have unique features that differ from the general breast cancers, including alterations
in cellular molecules, pathological bases, biological behavior, and a different prevention strategy. But the outcome of BRCA1/2 associated hereditary breast cancers still remains controversial; further studies are needed to elucidate the nature of BRCA1/2 associated hereditary breast cancers.
Project supported by the National Natural Science Foundation of China (No. 30772510) and the Joint Program of Ministry of
Health and Zhejiang Provincal Government of China (No. WKJ2006-2-008) 相似文献
9.
The endangered medicinal herb,Changium smyrnioides can only be found in deciduous forest gaps within the middle to northern subtropical broad-leaved evergreen forest zone of
China. The considerable plasticity of its shoot and root structure helps it to capture light more effectively in winter and
early spring, and to adapt to the soil moisture conditions in its narrow habitat. Another medicinal plant,Anthriscus sylvestris, is of similar economic importance but commonly distributed widely. In contrast toC. smyrnioides, it has low structural plasticity. It is also specialized to adapt to the moist and sunny environment, where habitat, such
as the banks of creeks and rivers, is abundant.
Project supported by the National Basic Research Program (973) of China (No. G2000046805) and the National Natural Science
Foundation of China (No. 39970058) 相似文献
10.
A rice mutant with Yaponica 9522 cultivar background Oryza sativa extraordinary glume 1 （Oseg 1） was identified from the M2 mutant pool mutagenized by ^60Co γ-ray. Compared with wild type plants, Oseg 1 developed longer empty glumes and rudimentary glumes. In some Oseg 1 mutants, the number of stamens of flowers was reduced and leaf-like lodicules occurred, and excessive lemma/palea-like organ could be observed in some mutant spikelets. This indicated that OsEG1 could regulate the development of rudimentary glumes, empty glumes, lemma/palea, lodicules, and stamens. Genetic analysis indicated that Oseg 1 came from a single recessive genetic locus. To clone OsEG1 gene, F2 population was constructed by a cross between Oseg 1 （Japonica） and Guangluai4 （Indica）. Using map-based cloning approach, OsEG1 was mapped on chromosome 4, between INDEL marker OS407 and WHM0466 with genetic distance of 2.0 cm and 1.0 cm, respectively. These results are useful for further cloning and functional analysis of the OsEG1 gene. 相似文献
11.
The long-arm and short-arm genes of fibroin light chain (L-chain) of silkworm,Bombyx Mori L., and the gene of human acidic fibroblast growth factor were cloned respectively and subsequently inserted into a transfer
vector pVL 1392 used as a tool to target the L-chain region of the silkworm genome. Genomic DNA from their offsprings was
extracted and the expected targeting was detected using polymerase chain reaction and DNA sequencing, as well as protein analysis.
The results showed that positive events occurred and that the FGF gene was integrated into the L-chain locus through homologous
recombination.
Project supported by the Scientific Research Foundation for Returned Overseas Chinese Scholars, Education Ministry of China
and the Natural Science Foundation of Zhejiang Province (No. 301306), China 相似文献
12.
The definition of J-integral of interfacial crack was introduced. The three-point bending tests were carried out to obtain the critical loading
values when the interfacial crack initiation occurred between coatings and substrates. The finite element analysis (FEA) was
adopted to analyze the stress distribution in the specimens and compute the J-integral of the interfacial crack between LX88A coating and Chinese Q345 steel. The results showed that the average value
of critical J-integral is 0.70 N/m, which can be taken as the fracture parameter to evaluate the interfacial fracture behavior for the
three-point bending specimens of LX88A coating/Q345 steel system.
Supported by Tianjin Natural Science Foundation (No.08JCYBJC09100), New Teacher Research Fund for the Doctoral Program of
Higher Education of China (No.20070056096) and New Century Outstanding Talented Person Plan of China.
XU Lianyong, born in 1975, male, Dr. 相似文献
13.
Tong-bao Liu Guo-qing Chen Hang Min Fu-cheng Lin 《Journal of Zhejiang University. Science. B》2009,10(6):434-444
Fasciclin family proteins have been identified as cell adhesion molecules in various organisms. In this study, a novel Magnaporthe oryzae fasciclin-like protein encoding gene, named MoFLP1, was isolated from a subtractive suppressive cDNA library and functionally analyzed. Sequence analysis showed that the MoFLP1 gene contains an open reading frame (ORF) of 1050 nucleotides encoding 349 amino acids with a calculated molecular weight
of 35.85 kDa and a pI of 7.76. The deduced MoFLP1 protein contains a 17-amino acid secretion signal sequence and an 18-amino
acid sequence with the characteristics of a glycosylphosphotidylinositol (GPI) anchor additional signal at its N- and C-terminuses,
respectively. Potential N-glycosylation sites and domains involving cell adhesion were also identified in MoFLP1. Sequence analysis and subcellular
localization by the expression of MoFLP1-GFP fusion construct in M. oryzae indicated that the MoFLP1 protein is probably localized on the vacuole membrane. Two MoFLP1 null mutants generated by targeted gene disruption exhibited marked reduction of conidiation, conidial adhesion, appressorium
turgor, and pathogenicity. Our results indicate that fasciclin proteins play important roles in fungal development and pathogenicity
in M. oryzae.
Project supported by the National Natural Science Foundation of China (No. 30870101) and the Public Welfare Profession (Agriculture)
Research Project (No. 200803008), China 相似文献
14.
Li-hua Zhang Gong-fu Ye Yi-ming Lin Hai-chao Zhou Qi Zeng 《Journal of Zhejiang University. Science. B》2009,10(2):103-111
Seasonal dynamics of total phenolics (TP), extractable condensed tannins (ECT), protein-bound condensed tannins (PBCT), fiber-bound
condensed tannins (FBCT), total condensed tannins (TCT), and protein precipitation capacity (PPC) in young, mature and senescent
branchlets of Casuarina equisetifolia were studied at Chishan Forestry Center of Dongshan County, Fujian Province, China. In addition, nitrogen contents of branchlets
at the different developmental stages were also determined. The contents of TP and ECT, and PPC in young branchlets were significantly
higher than those in mature and senescent branchlets through the season. However, PBCT contents were significantly higher
in senescent branchlets than those in young and mature branchlets; FBCT fluctuated with season. Young branchlets had the highest
N content, which decreased during branch maturity and senescence. The highest contents of TP and the lowest contents of TCT
and N in young and mature branchlets were observed in summer. There was a significant negative correlation between TP and
N contents. In contrast, TCT contents were positively correlated to N contents. Nutrient resorption during senescence and
high TCT:N ratios in senescent branchlets are the important nutrient conservation strategies for C. equisetifolia.
Project supported by the National Eleventh Five-year Key Project (No. 2006BAD03A14-01), Fujian Provincial Major Special Program
of Science and Technology (No. 2006NZ0001-2), the Program for New Century Excellent Talents in University (No. NCET-07-0725),
and the Program for Innovative Research Team in Science and Technology in Fujian Province University, China 相似文献
15.
A system dynamics approach to urban water demand forecasting was developed based on the analysis of urban water resources
system, which was characterized by multi-feedback and nonlinear interactions among system elements. As an example, Tianjin
water resources system dynamic model was set up to forecast water resources demand of the planning years. The practical verification
showed that the relative error was lower than 10%. Furthermore, through the comparison and analysis of the simulation results
under different development modes presented in this paper, the forecasting results of the water resources demand of Tianjin
was achieved based on sustainable utilization strategy of water resources.
Supported by National Natural Science Foundation of China (No.50578108), Doctoral Programs Foundation of Ministry of Education
of China (No.20050056016), National Key Program for Basic Research (“973” Program, No.2007CB407306-1), Science and Technology
Development Foundation of Tianjin (No.033113811 and No.05YFSYSF032), Educational Commission of Hebei Province (No.2008324)
and Tianjin Social Key Foundation (No.tjyy08-01-078).
ZHANG Hongwei, born in 1956, male, Dr. Prof. 相似文献
16.
Discovery of ultrahigh-T spinel-garnet granulite with pure CO<Subscript>2</Subscript> fluid inclusions from the Altay orogenic belt,NW China 总被引：1，自引：0，他引：1
We first report discovery of the spinel-garnet-orthopyroxene granulite with pure CO2 fluid inclusions from the Fuyun region of the late Paleozoic Altay orogenic belt in Central Asia, NW China. The rock is characterized
by an assemblage of garnet, orthopyroxene spinel, cordierite, biotite, plagioclase and quartz. Symplectites of orthopyroxene
and spinel, and orthopyroxene and cordierite indicate decompression under UHT conditions. Mineral chemistry shows that the
orthopyroxenes have high XMg and Al2O3 contents (up to 9.23 wt%). Biotites are enriched in TiO2 and XMg and are stable under granulite facies conditions. The garnet and quartz from the rock carry monophase fluid inclusions which
show peak melting temperatures of around-56.7°C, indicating a pure CO2 species being presented during the ultrahigh-T metamorphism in the Altay orogenic belt. The inclusions homogenize into a
liquid phase at temperatures around 15.3–23.8°C translating into CO2 of the order of 0.86–0.88 g/cm3. Based on preliminary minerals paragenesis, reaction textures and petrogenetic grid considerations, we infer that the rock
was subjected to UHT conditions. The CO2-rich fluids were trapped during exhumation along a clockwise P-T path following isothermal decompression under UHT conditions.
Project supported by the National Basic Research Program (973) of China (No. 2001CB409801), the Exemplary Young Teacher Education
and Scientific Research Award Plan of China University, and Postdoctoral Fund of China (No. 2003033033), Postdoctoral Fund
of Zhejiang Province, and Starting Fund of Education Ministry, China 相似文献
17.
Gao J Bao HY Xin MX Liu YX Li Q Zhang YF 《Journal of Zhejiang University. Science. B》2006,7(3):186-192
INTRODUCTION Bioflocculation is a dynamic process resulting from the synthesis of extracellular polymer by living cells. Since flocculation in microbial system was first reported by Louis Pasteur (Salehizadeh and Sho-jaosadati, 2001), bioflocculation has been investi-gated extensively and a correlation was established between the accumulation of extracellular biofloccu-lants and cell aggregation (Tenney and Verhoff, 1973). Bioflocculants are essentially polymers pro-duced by microorgan… 相似文献
18.
Different responses of two Mosla species to potassium limitation in relation to acid rain deposition
Meng Wang Bao-jing Gu Ying Ge Zhen Liu De-an Jiang Scott X. Chang Jie Chang 《Journal of Zhejiang University. Science. B》2009,10(8):563-571
The increasingly serious problem of acid rain is leading to increased potassium (K) loss from soils, and in our field investigation,
we found that even congenerically relative Mosla species show different tolerance to K-deficiency. A hydroponic study was conducted on the growth of two Mosla species and their morphological, physiological and stoichiometric traits in response to limited (0.35 mmol K/L), normal (3.25
mmol K/L) and excessive (6.50 mmol K/L) K concentrations. Mosla hangchowensis is an endangered plant, whereas Mosla dianthera a widespread weed. In the case of M. hangchowensis, in comparison with normal K concentration, K-limitation induced a significant reduction in net photosynthetic rate (P
n), soluble protein content, and superoxide dismutase (SOD) activity, but an increase in malondialdehyde (MDA) concentration.
However, leaf mass ratio (LMR) and root mass ratio (RMR) were changed little by K-limitation. In contrast, for M. dianthera, K-limitation had little effect on P
n, soluble protein content, SOD activity, and MDA concentration, but increased LMR and RMR. Critical values of N (nitrogen):
K and K:P (phosphorus) ratios in the shoots indicated that limitation in acquiring K occurred under K-limited conditions for
M. hangchowensis but not for M. dianthera. We found that low K content in natural habitats was a restrictive factor in the growth and distribution of M. hangchowensis, and soil K-deficiency caused by acid rain worsened the situation of M. hangchowensis, while M. dianthera could well acclimate to the increasing K-deficiency. We suggest that controlling the acid rain and applying K fertilizers
may be an effective way to rescue the endangered M. hangchowensis.
Project supported by the National Natural Science Foundation of China (No. 30570113) and the Research Fund for the Doctoral
Program of Higher Education (No. 20060335008), China 相似文献
19.
A fiberless seed mutant (fl) was identified in a commercial cotton (Gossypium hirsutum L.) variety Xu-Zhou 142 (FL). This phenotype is associated with lack of fiber cell initiation in the outer integument of
the ovule, as was characterized by analysis of genes related to fiber differentiation and development. Two genes, fl-E6 and
FL-E6, were cloned from fl-integument cells and FL-fiber or integument cells, respectively. Compared with FL-E6, fl-E6 showed
a dramatic change in nucleotide sequence: (1) FL-E6 contained a tandem repetitive sequence in which GGCTCA (Gly-Ser) is repeated
five times between the 82nd and the 93rd codon from the first ATG codon, while in fl-E6 the same sequence is repeated four
times; (2) The fl-E6 gene encodes a polypeptide of 241 amino acids but lacks two codons between the 90th and 93rd codon and
three between the 171st and 174th relative to FL-E6; (3) There are also 12 nucleotide substitutions which would result in
7 amino acid differences between fl-E6 and FL-E6. Analysis of RT-PCR and Northerm Blot showed that expression of the fl-E6
gene is suppressed in the fl-integument cells, but highly expressed in FL-fiber cells. The difference between fl-E6 and FL-E6
may be associated with lower expression of fl-E6 in the fl-integument cells. Searches of protein databases with the FL-E6
gene sequence showed similarity to the protein backbones of two arabinogalactan-proteins (AGPs), one from the filtrate of
suspension-cultured cells ofPyrus cornmunis (AGPPc2) and the other fromNicotiana alata (AGPNa2). Although the function of the FL-E6 protein in differentiation and development of cotton fiber cells is not known,
the data indicate that the mutation of fl-E6 gene from FL-E6 gene may inhibit the fiber cell initiation from epidermal cells
of the outer integument of the ovule.
Project supported by the National Natural Science Foundation of China (Nos. 39770473 and 30170058), National Program of Plant
Gene Transfer (J00-B-002-10), National High Technology Research and Development (863) Program of China (No. 2001AA212081),
and Research Program of Ministry of Education of China (No. 0114) 相似文献
20.
Yang Qi-xiang 《浙江大学学报(A卷英文版)》2002,3(1):94-99
This paper deals with the establishment ofT(1) theorem on Hardy spaceH
1 under condition of weak regularity. An operator or a function is identified on the basis of their wavelet coefficients which
are regrouped on some blocks. The actions of each block operator (pseudo-annular operator) on each block function (atom) are
exactly analyzed to establishT(1) theorem on Hardy space.
Project supported by the National Natural Science Foundation of China (No. 1000102) and Education Ministry of China. 相似文献