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1.
人参抗衰老作用的实验研究 总被引：4，自引：0，他引：4
徐承水 《赣南师范学院学报》2000,(3):54-56
采用人参水提取液 ,对小鼠的试验研究表明 :人参提取物能有效提高心、脑组织超氧化物歧化酶 (SOD)活性 ,降低过氧化脂质 (LP0 )和脂褐素含量 ,拮抗过氧化损伤 ,具有明显的抗衰老作用 相似文献
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目的观察复方松针提取液对小白鼠肝损伤的保护作用.方法分别以四氯化碳和醋氨酚造成小鼠急性损伤模型,用赖氏法测定小鼠血清SALT活力并观察醋氨酚中毒对小鼠生存率的影响.结果复方松针提取液能降低小白鼠SALT水平并显著提高醋氨酚中毒小鼠的生存率.结论复方松针提取液具有明显的保肝作用。 相似文献
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给予小鼠槐角提取液灌胃(100mg/10g,200mg/10g)可使小鼠血糖升高;连续用提取液灌胃,能预防小鼠实验性高胆固醇血症,并能明显提高小鼠运动耐力及抗缺氧能力.对小鼠体重影响不明显. 相似文献
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以热板法和扭体法为镇痛作用的药效学指标,考察中药陆英醇提法不同提取物的镇痛作用.中药陆英醇提法氯仿提取物能显著提高小鼠热板实验中的痛阈(P＜0.05),并减少小鼠扭体次数.中药陆英醇提法氯仿提取物具有显著的镇痛作用. 相似文献
5.
石耳多糖对小鼠免疫功能影响的初步研究 总被引：1，自引：0，他引：1
通过酸、碱、水三种方法提取石耳子实体多糖,分别腹腔注射(200mg/kg)免疫小鼠,观察三种提取物对正常小鼠免疫功能的影响。结果表明:水提法多糖得率最高(4.3%)。三种提取物均能显著提高(p<0.01)小鼠腹腔巨噬细胞吞噬能力;并使小鼠脾脏重量明显增加,而胸腺重量显著减轻。 相似文献
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《绵阳师范学院学报》2017,(2):52-55
采用灌胃法,进行了富硒杏鲍菇子实体对小鼠免疫力、抗氧化作用的药理试验,结果表明:富硒杏鲍菇组的小鼠脏器指数高于纯净水组、Na2Se O3组和不加硒普通杏鲍菇组的小鼠脏器指数;富硒杏鲍菇子实体的水提液、75%乙醇提取液、80%甲醇提取液对DPPH自由基的清除率分别达到72.69%、69.72%、68.31%,均显著高于市售普通杏鲍菇子实体和液体发酵富硒杏鲍菇菌丝体干粉对自由基清除率(P<0.01);富硒杏鲍菇子实体处理组的小鼠全血和肝脏中谷胱甘肽过氧化物酶(GSH-Px)和超氧化物歧化酶(SOD)活性显著高于对照组、Na2Se O3处理组、普通杏鲍菇组,而丙二醛(MDA)含量显著低于对照组、Na2Se O3处理组及普通杏鲍菇处理组.结论:富硒杏鲍菇可显著提高小鼠免疫力和抗氧化能力. 相似文献
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[目的]观察益智仁水提取物(A lp in ia oxyphylla M iq.fru it,AOF)对脑老化小鼠海马SOD活力及海马蛋白含量的影响.[方法]选用健康成年昆明种小鼠60只,雌雄各半,随机数字表法将其分成5组:对照组、模型组、脑复康组、AOF低剂量组、AOF高剂量组.用D-半乳糖皮下注射诱导小鼠脑老化模型,采用学习记忆行为训练和生化测定结合的方法,观察AOF对小鼠学习记忆能力的影响,测定海马组织超氧化物歧化酶(SOD)和海马蛋白含量.[结果]①AOF对脑老化小鼠学习记忆能力的影响:模型组达标所需训练次数显著高于对照组(p<0.01),AOF低、高剂量组达标所需训练次数显著低于模型组(p<0.01).②AOF对脑老化小鼠海马SOD活力的影响:模型组小鼠海马SOD活力显著低于对照组(p<0.05),AOF低、高剂量组小鼠海马SOD活力显著高于模型组(p<0.05).③AOF对脑老化小鼠海马蛋白含量的影响:与对照组比,模型组小鼠海马蛋白含量显著降低(p<0.01);与模型组相比,AOF低、高剂量组小鼠海马蛋白含量显著增高(p<0.01).[结论]AOF可提高脑老化小鼠海马SOD活力,增加海马蛋白含量,对D-半乳糖诱导脑老化小鼠的学习记忆障碍具有显著的改善作用. 相似文献
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目的:观察黄连素对高脂饲料诱导高脂血症小鼠脂质代谢与抗氧化水平的影响.方法:50只雄性昆明小鼠,随机分为对照组、模型组和黄连素低、中、高剂量组,每组10只.除了对照组饲喂基础饲料,其余四组均采用高脂日粮构建小鼠高血脂模型,建模同时,黄连素低、中、高剂量组分别灌胃50、100、200mg/(kg·d)进行干预,7周后测定小鼠血浆甘油三酯(TG)、总胆固醇(TC)、低密度脂蛋白胆固醇(LDL-ch)、高密度脂蛋白胆固醇(HDL-ch)、总蛋白(TP)、白蛋白(ALB)、丙二醛(MDA)含量以及丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)、超氧化物歧化酶(SOD)活力,并计算动脉硬化指数、肝脏与腹内脂肪相对重.结果:与模型组相比,黄连素可降低高脂日粮诱导的高脂血症小鼠的血脂水平、动脉硬化指数、MDA含量、血浆ALT和AST活性,提高血浆SOD活性、血浆TP、ALB浓度,同时降低肝脏和腹内脂肪相对重.结论:结果显示黄连素可以调节高脂血症模型小鼠的脂质代谢及其导致的动脉粥样硬化与肝损伤,同时具有一定的抵抗脂质过氧化作用. 相似文献
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海酮、女贞、黄杨叶片，富含酚类物质，在提取液中加入一定含量的PVPP，其SOD同工酶港显示清晰度明显提高。含4%PVPP的提取液制备的SOD粗提酶液的SOD同工酶谱带显示最为清晰，黄杨三条迁移率不同的同工醇带亦全部清晰显示。 相似文献
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研究不同浓度的豚草水浸液对小麦种子萌发及幼苗生长的影响.结果表明：小麦种子发芽率总体随着豚草水浸液浓度的升高而显著下降;对小麦幼苗初生根的伸长具有显著的抑制作用,但对小麦苗高的抑制作用程度稍弱;显著增强小麦幼苗茎叶的超氧化物歧化酶、过氧化物酶的活性.浓度效应明显. 相似文献
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We extracted six Hong Kong brown seaweed species with hot water for their antiviral properties. The cytotoxicity and antiviral activity of these extracts were tested by MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenlytetrezolium bromide] method, cytopathic effect reduction assay, and plaque reduction assay. The antiviral effect was further determined by flow cytometric analysis. The results showed that most of these extracts inhibited the propagation of herpes simplex virus types 1 and 2 (HSV-1 and HSV-2) standard strains with very low cytotoxicity to the host cells. The extracts ofHydroclathrus clathratus and Lobophora variegata showed more potential anti-HSV activities than the extracts of the other four seaweeds. They also had moderate anti-respiratory syncytial virus (RSV) activities but could not inhibit influenza A virus. Hydroclathrus clathratus was further extracted by diluted acid and alkali and the antiviral effects of the extracts were also detected. The result showed that the hot water extract contained the main carbohydrate components that exhibited the antiviral activities against various strains of HSV, including the acyclovir-resistant strain. HI-3, a compound fractionated from this hot water extract, showed a dose-dependent anti-HSV activity in flow cytometric analysis and plaque reduction assay. 相似文献
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刘晓蓉 《广东轻工职业技术学院学报》2002,1(1):19-21,50
用乙醇、乙醚和水3种溶剂对虎杖进行提取。采用一系列真菌和细菌，测试虎杖提取物的抑菌作用，与苯甲酸钠的抑菌效果相比较，并选择有代表性的食品进行防腐试验。结果表明：虎杖提取物对所试细菌有较强的抑制作用，而对霉菌的抑制作用却不如苯甲酸钠明显；对食品的防腐效果良好。并确定了虎杖提取物的最低抑菌浓度。 相似文献
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用热变性法或磷酸缓冲液法从拟南芥植物中粗提取超氧化物歧化酶（SOD）,再用丙酮法或硫酸铵分级盐析法纯化SOD．比较发现,热变性法粗提取的SOD回收率较高,而2种纯化方法的差异却无统计学意义;同时,还比较了用同一种方法所提取的拟南芥和大蒜之间的SOD活性,大蒜的SOD活性高于拟南芥． 相似文献
16.
钱和 《浙江大学学报(A卷英文版)》2004,5(6)
Dried ground leaves of Psidium guajava L. (guava) were extracted by water and aqueous ethyl alcohol 50% (1:10) ratio, and the total phenolic content in the extracts was determined spectrophotometrically according to Folin- Ciocalteu's phenol method and calculated as gallic acid equivalent (GAE). Remarkably high total phenolic content 575.3 (15.5 and 511.6(6.2 mg of GAE/g of dried weight material (for ethanol guava leaf extracts and water guava leaf extracts, respectively) were obtained. The antioxidant activity of lyophilized extracts was determined at ambient temperature by means of a 2,2-diphenyl-1-picrylhydryzyl (DPPH˙) colorimetry with detection scheme at 515 nm. The activity was evaluated by the decrease in absorbance as the result of DPPH˙ color change from purple to yellow. The higher the sample concentration used, the stronger was the free radical-scavenging effect. The results obtained showed that ascorbic acid was a substantially more powerful antioxidant than the extracts from guava leaf. On the other hand, the commercial guava leaf extracts and ethanol guava leaf extracts showed almost the same antioxidant power whereas water guava leaf extracts showed lower antioxidant activity. The parameter EC50 and the time needed to reach the steady state to EC50 concentration (TEC90) affected the antiradical capacity of the sample. The antioxidant efficiency (AE) has been shown to be a more adequate parameter for selecting antioxidants than the widely used EC50. This study revealed that guava leaf extracts comprise effective potential source of natural antioxidants. 相似文献
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Dried ground leaves ofPsidium guajava L. (guava) were extracted by water and aqueous ethyl alcohol 50% (1∶10) ratio, and the total phenolic content in the extracts
was determined spectrophotometrically according to Folin-Ciocalteu's phenol method and calculated as gallic acid equivalent
(GAE). Remarkably high total phenolic content 575.3±15.5 and 511.6±6.2 mg of GAE/g of dried weight material (for ethanol guava
leaf extracts and water guava leaf extracts, respectively) were obtained. The antioxidant activity of lyophilized extracts
was determined at ambient temperature by means of a 2,2-diphenyl-1-picrylhydryzyl (DPPH') colorimetry with detection scheme
at 515 nm. The activity was evaluated by the decrease in absorbance as the result of DPPH' color change from purple to yellow.
The higher the sample concentration used, the stronger was the free radical-scavenging effect. The results obtained showed
that ascorbic acid was a substantially more powerful antioxidant than the extracts from guava leaf. On the other hand, the
commercial guava leaf extracts and ethanol guava leaf extracts showed almost the same antioxidant power whereas water guava
leaf extracts showed lower antioxidant activity. The parameter EC50 and the time needed to reach the steady state to EC50 concentration (T
EC
50) affected the antiradical capacity of the sample. The antioxidant efficiency (AE) has been shown to be a more adequate parameter
for selecting antioxidants than the widely used EC50. This study revealed that guava leaf extracts comprise effective potential source of natural antioxidants. 相似文献
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以超声波提取法和大孔树脂对桑叶进行初步提取,得到三个提取物（Ⅰ、Ⅱ、Ⅲ）。设计用桑叶提取物作为阳离子识别试剂,利用紫外吸收光谱法研究了桑叶提取物与不同金属离子的络合选择性。由研究结果可知,提取物Ⅰ和Ⅱ对Fe^2＋、Fe^3＋具有良好的识别作用,提取物Ⅲ对Fe^3＋表现出良好的识别作用,表明桑叶的药效作用可能与人体内的铁存在重要关系。 相似文献