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1.
以蚕豆根尖为材料,研究洗手液对蚕豆根尖细胞的致畸效应,采用蚕豆根尖细胞的微核试验方法和染色体畸变试验方法,以不同浓度的洗手液为诱变剂,测定蚕豆根尖细胞的微核率、有丝分裂指数和染色体畸变率.结果表明:不同浓度的洗手液能诱发较高频率的微核,还能诱导染色体产生多种类型的染色体畸变.结论是洗手液具有一定的遗传毒性.  相似文献   

2.
以蚕豆为实验材料,研究了铽的遗传毒性、细胞毒性.1.蚕豆微核试验、染色体畸变试验结果显示:硝酸铽能诱发蚕豆根尖细胞产生微核以及诱发染色体畸变,且在3~24μg/mL浓度范围内呈剂量-效应关系,说明稀土元素铽对蚕豆根尖具有一定的遗传毒性.2.有丝分裂指数(MI)试验结果显示:低浓度下(3~12μg/mL)的硝酸铽能促进蚕豆根尖细胞有丝分裂,MI升高;但随着浓度的升高,MI下降.说明稀土元素铽对蚕豆根尖具有一定的细胞毒性.  相似文献   

3.
用秋水仙素、8-羟基喹啉、对二氯苯、低温(<4℃)处理蚕豆根尖,比较分析了它们诱发蚕豆根尖细胞间期核和染色体畸变的效应.  相似文献   

4.
蚕豆根尖微核技术在环境监测中的应用   总被引:5,自引:0,他引:5  
本实验以一些常用化学物质为研究对象,应用蚕豆根尖微核技术,以蚕豆根尖细胞微核率作为参照标准。通过对一些常用化学物质引起的水体污染及其遗传毒性的研究,探讨这些化学物质使用的安全性、合理性,蚕豆根尖微核技术在环境污染监测中的指示作用,及其在实际应用中推广的呵行性。  相似文献   

5.
以蚕豆为试料,研究了联苯胺对蚕豆的毒性效应.采用蚕豆根尖微核技术,以不同浓度的联苯胺为诱变剂,测定蚕豆根尖细胞的有丝分裂指数、微核率和胚根丙二醛含量.结果表明,联苯胺会造成蚕豆根尖细胞有丝分裂指数下降;在0~10mg·L-1试验浓度范围内,微核率随联苯胺浓度的升高而增加,但浓度为20mg·L-1时,微核率下降;联苯胺还能诱导产生多种类型染色体畸变.此外,联苯胺暴露8h,蚕豆胚根丙二醛含量随浓度的升高显著增加;暴露16h,蚕豆胚根丙二醛含量下降.表明不同浓度联苯胺对蚕豆有不同程度的损伤作用.  相似文献   

6.
以蚕豆根尖为材料,研究厕洁灵、丝毛剂和蔬果清洗剂对蚕豆根尖细胞的遗传毒性.利用不同浓度的厕洁灵、丝毛剂和蔬果清洗剂为诱变剂,测定蚕豆根尖细胞的微核率和染色体畸变率.结果:不同浓度的洗涤剂能诱发较高频率的微核,还能诱导染色体产生多种类型的染色体畸变.结论:三种洗涤剂对植物根尖细胞具有明显的致突变效应,有一定的遗传毒性;其中洁厕剂的毒性最大,丝毛剂次之,果蔬洗涤剂最弱.  相似文献   

7.
在农药普尔泰的正常使用浓度范围内,用不同浓度的普尔泰分别处理蚕豆根尖细胞4h和8h,监测其细胞微核诱变情况。结果表明,普尔泰对蚕豆根尖细胞微核的诱变,随着处理液浓度的增加、处理时间自延长,具有良好的剂量效应关系。用蒸馏水作对照处理,经t检验,差异极显著,说明普尔泰对蚕豆根尖细胞有明显的诱变作用。  相似文献   

8.
用化工厂废水、皮革厂废水和该两厂的混合废水分别对水稻种子和蚕豆根尖进行处理,观察水稻种子发芽率及幼苗的生长状况,以及蚕豆根尖细胞的微核率、异形核率和有丝分裂指数等情况.结果表明:化工厂废水对水稻种子的发芽率及幼苗的生长均有极其显著的抑制作用;皮革厂废水及皮革厂与化工厂的混合废水对水稻的发芽率及幼苗生长的影响均不显著.工业废水能诱发蚕豆根尖细胞产生较高的微核率和异形核率.皮革厂废水61.20‰,两厂混合废水为34.34‰,化工厂废水为10.10‰;异形核率:化工厂废水为10%,两厂混合废水为5.16%皮革厂废水为1.38%;化工厂废水及两厂混合废水对蚕豆根尖细胞分裂具有明显的抑制和危害,皮革厂废水对蚕豆根尖细胞有丝分裂指数影响不显著.说明化工厂和皮革厂废水对农作物具有明显的细胞遗传毒性.  相似文献   

9.
如今环境重金属污染已引起社会的广泛关注。本实验利用蚕豆根尖微核技术,研究了不同浓度的硫酸铜对蚕豆根尖细胞微核诱变的影响,从而从细胞的水平上来研究重金属的毒害机理,对进一步评价环境质量具有重要意义。  相似文献   

10.
对8种蚕豆根尖培养方法以完整组和去皮组进行比较研究。结果表明:去皮可明显提高初生根生长速度,并且根长的整齐度较为一致。研制了水培器,通过实验证明水培法是蚕豆根尖培养较为理想的一种方法。  相似文献   

11.
《城口厅志》应只有一种版本,即刘绍文主持的道光24年刻本。所见三种印本中,四川本印刷时间更早,书版的毁损程度较轻。城口本、重庆本印刷时间约晚50年,书版的毁损程度较重,但印刷质量较好,内容更完整,并增补有少量光绪年间的材料。城口本中的补正出自近人,讹误较多。  相似文献   

12.
文革期间的版权现象主要是废止与剥夺,它体现在有的作者的生存权利被剥夺;许多作者的作品所有权被剥夺;有些作者的创作权被剥夺;一些作家作品的发表权被剥夺;大多数作者的署名权被剥夺;大多数作者的报酬权被剥夺;一些作者对作品的改编权和修改权被侵害;一些作品的转载权被剥夺等方面。  相似文献   

13.
We examined how preschoolers coped with anger in interactions with well liked and not well liked peers. The free-play interactions of preschool-aged children ( M age = 66.27 months) were observed for 6 months. The frequency, causes, and intensity of children's anger, as well as their anger-related reactions, were compared for incidents provoked by peers who were "really liked" to those provoked by peers who were liked only "a little bit." Although there were no differences in the intensity of anger provocations by well liked and not well liked provocateurs, children's responses to provocations by well liked peers were more controlled than was the case for anger provoked by peers who were not well liked. In general, boys were more responsive to how they felt about the provocateur than were girls. These findings suggest that anger episodes with well liked children were less stressful than those with peers who were not well liked.  相似文献   

14.
分别用火焰原子吸收法和1,10-菲啰啉分光光度法测定铬铁废液中铁的总量.绘制了两种方法的标准曲线,测定了空白值、加标回收率和标准样品.两种方法的空白值都比较稳定,相关性以及斜率等数值都很接近,它们的准确度都比较好,相对标准偏差分别为1.2%和2.6%.两者的平均回收率分别为108%和94%,基本符合实验要求.因此,两种方法的测定结果都在真值允许范围内,它们的标准曲线,精密度和准确性都比较好.两种方法的结果通过t检验,无显著性差异.两者都适用于低浓度的铁溶液,但分光光度法灵敏度稍高.  相似文献   

15.
为了提高学生病理学教学质量,加强病理解剖学教学实验室建设,购买先进仪器和设备,组成国内一流实验教师队伍,制备大量标本、切片、照片及多媒体课件,病理案例收集,编写大量临床病理案例,国内外进修及国内外专家交流,增加学生感性认识,让学生理论联系实际,提高学习病理学的兴趣,能提供学生分析问题和解决问题的实践工作能力场所.让学生掌握病理学理论和实践知识.病理学实验教学是提高病理学教学质量的重要因素.  相似文献   

16.
分别以4种醛和联苯胺为原料,在氮气的保护下,合成了4种双希夫碱类热变色物质,并用红外光谱对其结构进行了表征,用显微熔点仪测定了其熔点、热变色温度.结果表明:合成的化合物结构和目标产物一致,且熔程较短,纯度较高,反应效果理想;该4种希夫碱热变色温度较高,均是性能优良的热变色物质.  相似文献   

17.
To assess the effects of preweaning and juvenile experiences on adult maternal behavior, two experiments were completed. In Experiment 1, pups were separated for long periods or short periods or were left undisturbed over the 1st week. Following weaning, the rats were exposed to foster pups over a 5-day period or were left undisturbed. There were no effects of early experience on juvenile behavior. In adult postpartum maternal tests, short separations reduced latencies to express maternal behaviors, whereas long separations combined with juvenile exposure to pups increased latencies. In Experiment 2, pups were separated for long periods or were left undisturbed over the 1st week. Following weaning, the rats were exposed to foster pups for 4 days or 8 days or were left undisturbed. Long separation retarded maternal responding in juvenile animals but had no effect on the adults. Four days of juvenile experience had negative effects on adult behavior, whereas 8 days did not have an effect.  相似文献   

18.
The effects of an interactive shared-reading intervention were evaluated with 3-to 4-year-old children from low-income families who attended subsidized child care. The children entered the program with oral language skills that were significantly below age-level as measured by standardized tests. Children were pretested and randomly assigned to 1 of 4 conditions: (a) no treatment control, (b) a school condition in which children were read to by their teachers in small groups, (c) a home condition in which children were read to by their parents, and (d) a combined school plus home condition. Parents and teachers were trained in a specific form of interactive reading via an instructional videotape. The intervention was conducted for 6 weeks, after which children were posttested on standardized measures of oral language, and language samples were obtained during a shared-reading assessment. Significant effects of the reading intervention were obtained at posttest and were largest for children in conditions involving home reading.  相似文献   

19.
罗马帝国时期,君主个人权利凌架于国家之上,无道之君众多,他们不关心国家的隐定与发展,只关心吃喝玩乐。许多公民追随统治者,恣情游乐,成为畸生阶层。由此,导致了社会上以奢侈消费为特征的个人消费极度化膨胀,它的风行使得阶级冲突日益尖锐,政治日益黑暗,道德日见沦丧,从而加速了西罗马帝国的衰亡。  相似文献   

20.
Sixty-five sexually abused children were administered the Children's Depression Inventory (CDI) following investigation of their abuse to determine the relationship of victims' depression and suicidal ideation with victims' age, post-investigation residence, nature of the abuse, and mothers' belief of victim and compliance with treatment recommendations. Results indicated that older victims were more likely to describe themselves as being depressed. In addition, older victims and those whose mothers were less compliant were more likely to have CDI scores that were above the 90th percentile reported by Smucker, Craighead, Craighead, and Green (1986). Suicidal ideation was more likely in victims who were older, whose mothers were less compliant, who were not removed from their homes, and who had been molested by a family member.  相似文献   

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