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1.
Production of bacterial blight resistant lines from somatic hybridization between Oryza sativa L. and Oryza meyeriana L. 总被引：2，自引：0，他引：2
Novel bacterial blight (BB) resistance gene(s) for rice was (were) introduced into a cultivated japonica rice variety Oryza sativa (cv. 8411), via somatic hybridization using the wild rice Oryza meyeriana as the donor of the resistance gene(s). Twenty-nine progenies of somatically hybridized plants were obtained. Seven somatically hybridized plants and their parents were used for AFLP (amplified fragment length polymorphism) analysis using 8 primer pairs. Results confirmed that these plants were somatic hybrids containing the characteristic bands of both parents. The morphology of the regenerated rice showed characters of both O. sativa and O. meyeriana. Two somatic hybrids showed highest BB resistance and the other 8 plants showed moderate resistance. The new germplasms with highest resistance have been used in the rice breeding program for the improvement of bacterial blight resistance. 相似文献
2.
《湖南师范大学社会科学学报》1975,(2)
当前我国农业战线正在大力开展水稻杂种优势利用的科学实验。育种实践要求深入了解受精过程。为了适应这种需要,本文详细报导了在四个水稻品种中观察到的受精过程。水稻的受精作用有如下的特点:1)多条花粉管参与受精作用的现象很显著,与此相联系的是,多精受精的情况见到较多;2)在受精过程中,花粉管物质不断进入胚囊,并堆积在卵细胞的一侧;当合子发育成原胚时,则逐渐消失;看来,它们很可能参与了原胚的形成。以上两个问题是受精生物学中的重大理论问题。因此,水稻的受精作用不但具有实际意义,而且具有重要的理论意义,值得作进一步的研究。 相似文献
3.
INTRODUCTIONCorrectlypinpointingsplicing sitesingenomicDNAsequencesisnotaneasytask ,whichisofgreatimportancetothegenomeannotationandgenefinding .Intronsaregenerallydividedinto3classes,namelycalssI,classIIandcommonnu cleuspre mRNA .IntronofclassIandIIcango… 相似文献
4.
ALAM M.F. KHAN M.R. NURUZZAMAN M. PARVEZ S. SWARAZ A.M. ALAM I. AHSAN N. 《Journal of Zhejiang University. Science. B》2004,(4)
INTRODUCTIONAlthoughriceisanaturallyself-pollinatedcrop,strongheterosisisobservedintheirF1hybrids.HeterosisorhybridvigorismanifestedasimprovedperformanceforF1hybridsgeneratedbycrossingtwoinbredparents.Heterosiscanbedefinedquan-titativelyasanupwarddeviationofthemid-parent,basedonthemeanvaluesofthetwoparents(JohnsonandHutchinson,1993).Heterosismaybepositiveornegative.Dependinguponbreedingob-jectives,bothpositiveandnegativeheterosisareusefulforcropimprovement.Ingeneral,positiveheterosisisd… 相似文献
5.
INTRODUCTIONNongreenplastidsofheterotrophictissuesarecarbohydrate importingorganellesand ,inthecaseofamyloplastsofstoragetissues,thesiteofstarchsynthesis.Investigationofwholetissuesfromavarietyofstarchsynthesizingcropplantsindicatedthathexoseunitswereimpo… 相似文献
7.
NaCl胁迫对水稻（Oryza sativa）种子萌发和幼苗生长的影响 总被引：1，自引：0，他引：1
采用水培法,研究不同浓度（0、50、100、150、200、250、300、350、400、450、500mmol／L）的NaCl胁迫对水稻种子萌发,胚根、胚芽和幼苗生长的影响。结果表明：种子的发芽势、发芽率、都随着NaCl胁迫浓度的升高而降低,300mmol／L以上种子发芽率为0;胚根长,胚芽长也随着NaCl胁迫浓度的升高而降低,浓度大于100mmol／L时有显著（p〈0．05）影响。水稻幼苗的根长、株高、株鲜重、根干重以及茎干重也随NaCl胁迫浓度的升高呈逐渐降低的趋势,当浓度大于250mmol／L时影响尤为显著,大于300mmol／L时,植株严重失水,萎蔫,枯黄,死亡。研究结果说明NaCl胁迫对水稻种子的萌发和幼苗的生长有严重的抑制作用。 相似文献
8.
水杨酸对NaCl胁迫下水稻(Oryza Saliva L.)幼苗生理活性的影响 总被引：1，自引：0，他引：1
用0．2％水杨酸处理1．2％NaCl胁迫下水稻种子，测定其以下几个生理指标，种子的发芽率，发芽势和幼苗中的酯酶，过氧化氢酶以及抗坏血酸的含量，实验结果显示：外源性水杨酸能够相对提高NaCl胁迫下水稻种子萌发后幼苗的过氧化物酶活性和抗坏血酸的含量，同时也能提高水稻种子的发芽率，发芽势，但对酯酶活性影响不大，研究表明水杨酸能提高水稻种子萌发后幼苗的抗盐性，并使水稻种子出苗整齐，一致。 相似文献
9.
INTRODUCTION Leaf senescence is a kind of programmed cell death (PCD) in plants that is highly controlled by a genetically programmed process involving degrada-tion of cellular structures, decline of photosynthesis massive generation of active oxygen species (AOS) and mobilization of nutrients (Nam, 1997; Lim et al.,2003). Many environmental factors have been shown to play a role in causing the photosynthetic decline to lead to premature senescence. Leaves exposed to ultraviolet radia… 相似文献
10.
Li FZ Jin SH Hu GC Fu YP Si HM Jiang DA Sun ZX 《Journal of Zhejiang University. Science. B》2005,6(8):803-811
A rice pse(t) (premature senescence, tentatively) mutant line, was isolated from 4500 independent T-DNA inserted transgenic lines. The symptoms of premature senescence appeared more severely than those of the control plants (Zhonghua 11,japonica) at the last development stage. To characterize the mutant and provide basic information on the candidate genes by mapping to a physical region of 220-kb, experiments were carried out in two phytotrons under controlled temperature of 24 ℃ and28 ℃, respectively. The content of chlorophyll, soluble protein and MDA (malondialdehyde), net photosynthesis, the antioxidant enzyme activities of SOD (superoxide dismuase) (EC 1.15.1.1) and POD (peroxidase) (EC 1.11.1.7) and the peptidase activities of leaves were measured from top to bottom according to the leaf positions at the flowering stage. Compared with the control plant,the mutant showed the following characteristics: (1) Higher net photosynthesis rate (Pn) appeared in the 1 st and 2nd leaves, contents of chlorophyll and soluble protein were also higher in the 1 st leaf; (2) The activities of SOD, POD and peptidase were higher according to the leaf position from top to bottom; (3) The symptom of premature senescence was accelerated in the mutant at 28℃treatment. The MDA content and the SOD and POD activities between the 24 ℃ and 28 ℃ treatment mutants were not significantly different. Content of chlorophyll and soluble protein of leaves mutant decreased rapidly at 28 ℃ treatment. The results show that pse(t) is sensitive to high temperature. The probable function of PSE(T) is discussed. 相似文献
11.
A rice mutant with Yaponica 9522 cultivar background Oryza sativa extraordinary glume 1 （Oseg 1） was identified from the M2 mutant pool mutagenized by ^60Co γ-ray. Compared with wild type plants, Oseg 1 developed longer empty glumes and rudimentary glumes. In some Oseg 1 mutants, the number of stamens of flowers was reduced and leaf-like lodicules occurred, and excessive lemma/palea-like organ could be observed in some mutant spikelets. This indicated that OsEG1 could regulate the development of rudimentary glumes, empty glumes, lemma/palea, lodicules, and stamens. Genetic analysis indicated that Oseg 1 came from a single recessive genetic locus. To clone OsEG1 gene, F2 population was constructed by a cross between Oseg 1 （Japonica） and Guangluai4 （Indica）. Using map-based cloning approach, OsEG1 was mapped on chromosome 4, between INDEL marker OS407 and WHM0466 with genetic distance of 2.0 cm and 1.0 cm, respectively. These results are useful for further cloning and functional analysis of the OsEG1 gene. 相似文献
12.
A rice mutant with Japonica 9522 cultivar background Oryza sativa extraordinary glume 1 (Oseg 1) was identified from the M2 mutant pool mutagenized by ~(60)Coγ-ray.Compared with wild type plants,Ose9 1 developed longer empty glumes and rudimentary glumes.In some Oseg 1 mutants,the number of stamens of flowers was reduced and leaf-like lodicules occurred,and excessive lemma/palea-like organ could be observed in some mutant spikelets.This indicated that OsEG1 could regulate the development of rudimentary glumes,empty glumes,lemma/palea,lodicules,and stamens.Genetic analysis indicated that Oseg 1 came from a single recessive genetic locus.To clone OsEG1 gene,F_2 population was constructed by a cross between Ose9 1(Japonica)and Guangluai4(Indica).Using map-based cloning approach,OsEG1 was mapped on chromosome 4,between INDEL marker OS407 and WHM0466 with genetic distance of 2.0 cm and 1.0 cm,respectively.These results are useful for further cloning and functional analysis of the OsEG1 gene. 相似文献