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1.
研究目的：优化获得血红密孔菌（P.sanguineus）的最佳培养基组成,提高耐热漆酶的产量。创新要点：获得了目前文献报道的最高水平的漆酶活力。研究方法：通过单因素试验研究了不同培养基（番茄汁、麦麸、麦芽提取物和葡萄糖细菌蛋白胨培养基）和不同组合诱导剂（大豆油、阿魏酸、没食子酸、二甲基苯胺、酸性蓝62和活性蓝19分别与硫酸铜组合诱导剂）对P.sanguineus产耐热漆酶的影响。在此基础上采用中心组合试验设计,进一步研究了番茄汁培养基结合硫酸铜和大豆油组合诱导剂对P.sanguineus产耐热漆酶的影响。利用SAS10.0和响应面分析方法对试验结果进行了统计分析和建立回归模型。重要结论：通过中心组合设计优化得出P.sanguineus产耐热漆酶的最优培养基条件：以36.8%番茄汁为培养基,以3 mmol/L硫酸铜和1%大豆油作为组合诱导剂。该条件下在10 L搅拌槽生物反应器中漆酶活力达到了143 000 IU/L（2,2＇-联氮双（3-乙基苯并噻唑啉-6-磺酸）为底物,pH值为3.0）。  相似文献   

2.
利用蒸发法制备了含有TiO_2的自清洁涂料,考察了相关条件对涂层材料自清洁效果的影响。结果显示:随着TiO_2含量的增加,涂层干燥时间逐渐提高,涂层的水接触角增大;TiO_2含量为15%时涂层的光催化性能最强;含TiO_2的涂层对灰尘、油污、咖啡都具有较好的自清洁效果,其机械性能满足涂料应用的一般性要求。  相似文献   

3.
Renewed interest in natural materials as food flavors and preservatives has led to the search for suitable essential oils. Moringa oleifera seed essential oil was extracted by solvent-free microwave and hydrodistillation. This study assessed its chemical constituents. Cytotoxicity of the oils was investigated using hatchability and lethality tests on brine shrimps. A total of 16 and 26 compounds were isolated from the hydrodistillation extraction (HDE) and solvent-free microwave extraction (SME) oils, respectively, which accounted for 97.515% and 97.816% of total identifiable constituents, respectively. At 24 h when the most eggs had hatched, values of the SME (56.7%) and HDE (60.0%) oils were significantly different (P<0.05) from those of sea water (63.3%) and chloramphenicol (15.0%). Larva lethality was different significantly (P<0.05) between HDE and SME oils at different concentrations and incubation periods. The median lethal concentration (LC50) of the oils was >1000 mg/ml recommended as an index for non-toxicity, which gives the oil advantage over some antioxidant, antimicrobial, therapeutic, and preservative chemicals.  相似文献   

4.
Lithium-zinc ferrite hollow microspheres (LiZn FHMs) containing special surface crystals were synthesized by self-reactive quenching technology. The samples were heat-treated at 1200 °C and held for 4 h. The influence of the heat-treatment on LiZn FHMs was studied. The results show that the surface of hollow microspheres is smooth without heat-treatment. The phase components are Fe2O3, Fe3O4, Li0.435Zn0.195Fe2.37O4, and Li0.5Fe2.5O4. The minimum reflectivity is ?13.5 dB, and the corresponding frequency is 7.5 GHz. The effective absorption band lower than ?10 dB is 6.2–8.5 GHz, and the bandwidth is 2.3 GHz. After heat-treatment, crystals on the surface of hollow microspheres grow significantly. Multiple-shape micro-nano crystals containing triangular, polygonal, and irregular crystal are generated. However, the phase composition does not change. The real part of the permittivity (ε′), the imaginary part of permittivity (ε″), the real part of permeability (μ′), and the imaginary part of permeability (μ″) all increase, and the microwave absorption properties at low frequency are significantly increased, with the absorption peak moving to a lower frequency range. The minimum reflectivity is ?26.5 dB, and the corresponding frequency changes to 3.4 GHz. The effective absorption band is 2.6–4 GHz, and the bandwidth is 1.4 GHz.  相似文献   

5.
The work is intended to achieve optimum culture conditions of α-galactosidase production by a mutant strain ,Aspergillusfoetidus ZU-GI in solid-state fermentation （SSF）. Certain fermentation parameters involving moisture content, incubation temperature, cultivation period of seed, inoculum volume, initial pH value, layers of pledget, load size of medium and period of cultivation were investigated separately. The optimal cultivating conditions of α-galactosidase production in SSF were 60% initial moisture of medium, 28 ℃ incubation temperature, 18^h cultivation period of seed, 10% inoculum volume, 5.0-6.0 initial pH of medium, 6 layers of pledget and 10 g dry matter loadage. Under the optimized cultivation conditions, the maximum α-galactosidase production was 2037.51 U/g dry matter near the 144th hour of fermentation.  相似文献   

6.
The hepatoprotective and antioxidant activities of the n-butanol extract of Rubus parvifolius L. (RPL), a widely used medicinal plant, were evaluated. Results demonstrated that RPL extract possessed pronounced hepatoprotective effects against carbon tetrachloride (CCl4)-induced hepatic injury in mice, which was at least partially attributed to its strong antioxidant capacity. Treatment with RPL extract markedly attenuated the increases in serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels caused by CCl4 intoxication. It also significantly prevented the decrease in superoxide dismutase (SOD) activity and the increase in malondialdehyde (MDA) content of liver tissue. Meanwhile, histopathological changes of hepatic damage were also remarkably ameliorated. Phytochemical analysis based on high-performance liquid chromatography/tandem mass spectrometry (HPLC-MS/MS) revealed the presence of various phenolic compounds, including caffeic acid conjugates, ellagic acid glycosides, and flavonol glycosides, which might be responsible for the hepatoprotective and antioxidant activities of RPL.  相似文献   

7.
采用正交试验法研究板材变形程度、润滑条件、轴向加裁力大小和变形速度对AZ31B镁合金挤压板材室温拉楔性能的影响,结果表明:变形程度对拉楔试验顺利完成的影响最为显著,其次是润滑条件和轴向加载力大小,采用固体润滑剂的润滑效果优于油脂类润滑剂,且轴向加载力越大对拉楔试验成功越有利;而变形速度超过1 mm/min时,其影响效果不明显.在拉楔系数0.883,轴向加载力为拉楔力的1.759倍,使用固体肥皂作为润滑刑,取得了良好试验效果.  相似文献   

8.
油脂既是日常生活的必需品,也是重要的工业原料。天台山油脂植物资源丰富,大约有293种,本文对其中的99种植物的含油率和油脂用途作了简要介绍,为合理利用这种资源提供了科学依据。  相似文献   

9.
An incubation study was conducted to investigate the effects of metsulfuron-methyl herbicide on the soil microbial biomass in loamy sand soil. The herbicide was applied to the soil at four concentrations: control, 0.01, 0.10, and 1.00 μg·g−1 soil. Determinations of microbial biomass-C and microbial biomass-N contents were carried out 1, 3, 5, 7, 10, 15, 25, and 45 days after herbicide application. In comparison to untreated soil, the microbial biomass-C and biomass-N decreased significantly in soils treated with herbicide at concentrations of 0.1 and 1.0 μg·g−1 soil within the first 7 days of incubation. The application of metsulfuron-methyl herbicide to the soil reduced the Cmic/Corg and Nmic/Ntotal percentages, which decreased with increasing application rate of metsulfuron-methyl herbicide. Compared to the untreated control, a marked increase in the microbial biomass C: N ratio was observed in the herbicide treated soil. This effect was transitory and was significant only at the higher rates of metsulfuron-methyl. Project supported by NSFC (No. 49871044) and the Laboratory of Materials Cycling in Pedosphere, Academia Sinica.  相似文献   

10.
INTRODUCTION P450 enzymes are found throughout nature and can catalyze the oxidation of various chemicals (Ortiz de Montellano, 1995). Because of their important roles in these processes, numerous systems have been developed for the expression of P450s in vitro (Helvig and Capdevila, 2000; Nakamura et al., 2001; Deeni, 2001). But P450 enzymes fermentation levels in the microbial system are too low to catalyze the reactions for industrial preparations. Therefore, it is necessary to impr…  相似文献   

11.
文章基于密度泛函理论,研究了本征及Nb,Fe单原子掺杂单层MoSe2的电子结构及光学性质。计算发现,本征单层MoSe2和Nb-MoSe2为直接带隙半导体,Fe-MoSe2为间接带隙结构;Fe-MoSe2较本征单层MoSe2导电性大大提高,实现了由半导体向半金属的过渡。由态密度分析得出了本征及Nb,Fe单原子掺杂单层MoSe2能量状态主要由Mo 4d,Se 4p轨道电子所贡献的结论,并对各原子掺杂体系轨道电子的能量贡献和掺杂类型做了探讨。此外,还详细分析了费米能级附近的自旋态密度、杂质带、磁性之间的联系。光学性质方面,比较了本征单层MoSe2与各掺杂体系的复介电常数和光吸收系数,在红外光区Fe-MoSe2的吸收系数高于本征单层MoSe2。本征单层MoSe2的光吸收系数为9.69×104 cm-1,是区域最大吸收峰。上述研究表明,通过对单层MoSe2的Nb,Fe掺杂可使电子输运特性得到了增强,为高活性自旋电子和光电子器件设计和研究开辟了新的前景。  相似文献   

12.

Objective  

Human embryonic stem cells (hESCs) have recently been reported as an unlimited source of mesenchymal stem cells (MSCs). The present study not only provides an identical and clinically compliant MSC source derived from hESCs (hESC-MSCs), but also describes the immunomodulative effects of hESC-MSCs in vitro and in vivo for a carbon tetrachloride (CCl4)-induced liver inflammation model.  相似文献   

13.
Strain improvement and medium optimization to increase the productivity of spiramycin were carried out. Of oil tolerant mutant strains screened, one mutant,Streptomyces ambofaciens XC 2–37, produced 9% more spiramycin than the parent strainS. ambofaciens XC 1–29. The effects of soybean oil and propyl alcohol on spiramycin production withS. ambofaciens XC 2–37 were studied. The potency ofS. ambofaciens XC 2–37 was improved by 61.8% with addition of 2% soybean oil in the fermentation medium and 0.4% propyl alcohol at 24 hours after incubation. The suitable time for feeding propyl alcohol is at 24 hours after incubation in flask fermentation and at 20 hours after incubation in fermentor fermentation. The new process withS. ambofaciens XC 2–37 was scaled up for industrial scale production of spiramycin in a 60 m3 fermentor in Xinchang Pharmaceutical Factory, Zhejiang Medicine Company, Ltd., China, and the potency and productivity of fermentation were improved by 42.9%.  相似文献   

14.
研究目的：研究方法：重要结论：确定氢氧化钠溶液化学吸收硫酰氟的传质反应动力学区域,建立增强因子模型。研究气液传质测定设备双搅拌釜中氢氧化钠溶液化学吸收硫酰氟的过程,并结合实验研究与理论分析建立了增强因子模型。基于氢氧化钠溶液化学吸收硫酰氟过程的实验研究,确定了氢氧化钠溶液化学吸收硫酰氟的传质反应动力学区域为快速拟一级反应。得到了298K下其二级反应速率常数为1．44m3／（mol·s）,并建立了增强因子模型E=68．08CBL1/2,为脱除熏蒸后残留硫酰氟的工业化应用提供了理论依据。  相似文献   

15.
This article examines the effects of one year of exposure to “Learning to Read in a Healing Classroom” (LRHC) on the reading and math skills of second- to fourth-grade children in the low-income and conflict-affected Democratic Republic of the Congo. LRHC consists of two primary components: teacher resource materials that infuse social-emotional learning principles into a reading curriculum and collaborative school-based teacher learning circles to exchange information about and solve problems in using the teacher resource materials. To test the impact of LRHC on children's reading and math skills, 40 school clusters containing 64 schools and 4,465 students were randomized to begin LRHC in 2011–2012 or to serve as wait-list controls. Hierarchical linear models (students nested in schools, nested in school clusters) were fitted. Results indicate marginally significant positive impacts on children's reading scores (dwt = .14) and geometry scores (dwt = .14) but not on their addition/subtraction scores. These results should be treated with caution given the reported significance level of p < .10. The intervention had the largest impacts on math scores for language minority children and in low-performing schools. Research, practice, and policy implications for education in low-income conflict-affected countries are discussed.  相似文献   

16.
A novel β-PbO2 anode modified with fluorine resin was developed for typical pollutant electrocatalytic degradation and wastewater treatment. Various operating parameters such as applied voltage (3.5–10.5 V), pH (2–6), salinity of the electrolyte (0.5–2 g/L K2SO4) and initial phenol concentration (100–400 mg/L) were investigated to explore the electrocatalytic ability of the anode by taking phenol as sample. A preliminary study on dyeing wastewater treatment by this method indicated that the biodegradability could be increased to suit subsequent biological treatment. The stability of the anode has been proved to be high against acidity. The anode showed promising application for treatment of wastewater, especially of high salinity and high acidity wastewater.  相似文献   

17.
分析和探讨近年来在润滑材料和润滑技术方面的研究进展。润滑材料方面有绿色润滑油、离子液体、液晶润滑以及固体添加剂在润滑脂中的应用等;润滑技术方面有纳米润滑技术、颗粒流润滑技术和油雾润滑技术等。  相似文献   

18.
A novel nano-TiO2-xNx composite was used as photocatalyst and added to the interior wall paint. The average diameter of nano-TiO2-xNx was about 20 nm. The majority crystal component of the sample was anatase and its optical absorption edge was shifted from 387 nm to 520 nm significantly. Nano-composite paint containing different dosage of nano- TiO2-xNx was investigated to study the properties of formaldehyde decomposition in the air. Testing results show that the formaldehyde decomposition ratio of that nano-paint can almost reach above 80%, especially for that of the paint containing 3% （w/w） nano-TiO2-xNx which exceeded 90%. The primary investigation on the reaction kinetics of photocatalytic formaldehyde decomposition indicated that the experiment data well fit the model of first-order reaction kinetics.  相似文献   

19.
Ultrafine γ-Al2O3 particles are synthesized in Triton X-100/n-hexanol/cyclohexane/water water in oil(w/o) microemulsion by mixing two separately prepared microemulsions containing Al(NO)3 and (NH4)2CO3 respectively. The ultrafine Al2O3 particles are characterized by transmission electron microscopy (TEM) and X-ray diffraction (XRD) and their size and distribution are measured. The effects of water, surfactant and reactant concentrations on the particle size and distribution are studied. The results show that the particle size and distribution can be changed by varying the preparation conditions, and the size of the microemulsion droplets has a controlling effect on the size of the particles. A possible mechanism of ultrafine particles (UFPs) prepared by microemulsions is proposed.  相似文献   

20.
Chemistry behind the life of a transformer   总被引：3，自引：0，他引：3  
Conclusion Scientific assessment of the insulation conditions rather than years of service determine the remnant life of the transformer. Insulation age of a transformer is exclusively decided by the life of cellulosic materials. Extent of degradation of cellulosic materials can be quantified by measuring dissolved CO2 gas content in oil, degree of polymerization of paper and furan content in oil. Transformer life is shortened by a number of events. Taking action to prevent failure from any of these causes is a method of life extension. Maintaining the insulation system in good order and controlling loads by the use of dynamic loading of the equipment make it possible to improve the utilization of the existing transformer capacity.  相似文献   

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