共查询到20条相似文献，搜索用时 93 毫秒
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用ＰＣＲ方法改造鲑鱼生长激素（ＧＨ）ｃＤＮＡ,在成熟蛋白编码序列５′端前加入ＥｃｏＲⅠ位点,改造后的ｃＤＮＡ通过平端连接插入质粒ＹＥＰＭ０４,使鲑鱼ＧＨ基因置于酵母ＭＦα１因子分泌肽前导序列后、并在ＭＦα启动子调控之下,成为大肠杆菌酵母穿梭质粒ＹＥＰＭ０４ｆＧＨ．重组子酶切分析表明,鲑鱼ＧＨ基因正向插入质粒ＹＥＰＭ０４的ＭＦα启动子和分泌肽序列之后 相似文献
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贾玉友 《中学数学教学参考》2001,(9)
定理 Ｈ为三棱锥Ａ ＢＣＤ底面的重心 ,Ｇ为ＡＨ上的点 ,且 ＡＧＡＨ =ｋ ,△Ｂ′Ｃ′Ｄ′为过Ｇ的任一截面 (如图 ) ,则ＡＢＡＢ′ ＡＣＡＣ′ ＡＤＡＤ′=3ｋ .证明 :如图 ,Ｈ为△ＢＣＤ的重心 ,则ＶＡ ＢＨＣ =ＶＡ ＣＨＤ =ＶＡ ＤＨＢ=13 ＶＡ ＢＣＤ.∵ ＶＡ Ｂ′ＧＣ′ＶＡ ＢＨＣ=ＡＢ′·ＡＣ′·ＡＧＡＢ·ＡＣ·ＡＨ =ＶＡ Ｂ′ＧＣ′13ＶＡ ＢＣＤ,ＡＧＡＨ=ｋ ,∴ ＶＡ Ｂ′ＧＣ′ＶＡ ＢＣＤ=ｋ3·ＡＢ′·ＡＣ′ＡＢ·ＡＣ ,同理可得类似的两个等式 .三式相加 ,有ＡＢ′·ＡＣ′·ＡＤ′ＡＢ·ＡＣ·ＡＤ =ＶＡ Ｂ′Ｃ′Ｄ… 相似文献
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家蚕核型多角体病毒p35基因的核苷酸序列分析 总被引：1，自引：0，他引：1
对家蚕核型多角体病毒苏州株（ＢｍＮＰＶｓｕ）ｐ３５基因的序列分析表明：ＢｍＮＰＶｓｕ ｐ３５编码序列为８９７ｍｔ,编码２９８ａａ。同源性分析表明：ＢｍＮＰＶｓｕ ｐ３５与ＢｍＮＰＶＴ３、ＡｃＮＰＶ 、ＳＩＮＰＶ在核苷酸水平上同源性分别为９９．５％、９５．１％、８９．３％,在氨基酸水平上的 性分别为９８．７％、８９．４％、７６．４％,显示了杆状病毒ｐ３５基因在进化上的保守性。ＢｍＮＰＶＴ３中位的Ｎ１ 相似文献
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陆军 《常熟理工学院学报》1996,5(4):40-49
ｐＧＡ４６－４是一个带有谷氨酸棒杆菌妄动功能片断的质粒。用ＰＣＲ技术从ｐｇａ４６－４中扩增该启动子片断，将该启动子Ｔ４噬菌体Ｐ３２基因的终止子克隆到大肠杆菌质粒ｐＫ１８上，再接入棒状杆菌粒ｐＸＺ１０１４２构建一穿梭表达载体。 相似文献
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雪花莲外源凝集素（ＧＮＡ）是一类能特异地和甘露糖残基结合的蛋白质成分，对人畜毒性极低，但对同翅目昆虫有强烈的抗性。为利用雪花链外源集素对同翅目害虫的抗性，将雪花莲外源凝集素基因置于Ｕｂｉ启动子和Ｔｎｏｓ终止子的控制之下，插入ｐＣＡＭＢＩＡ３３００的ＳｍａＩ位点，构建了可用一农杆菌转化的植物中间表达载体－ｐＣＵＧＡＮ－Ｂａｒ．将ｐＣＵＧＡＮ－Ｂａｒ电击转入农杆菌，得到了带有ｐＣＵＧＡＮ－Ｂａｒ的农杆 相似文献
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摄录一体机电池组的修复刘黎明目前,一般学校使用的摄录一体机主要是松下公司的ＶＨＳ格式,如Ｍ－３０００、Ｍ－８０００、Ｍ－９０００、ＡＧ－４５５、ＡＧ－ＤＰ２００等。这些机型使用的电池组为ＶＷ－ＶＢＦ２Ｅ、ＶＷ－ＷＢＭ１０ＥＮ、ＬＣＳ－２０１２ＤＣＹ、... 相似文献
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用苜蓿丫纹夜蛾核型多角体病毒（ＡｃＭＮＰＶ）ｐ７４基因的部分片段标记探针,通过ＳｏｕｔｈｅｒｎＢｌｏｔｉｎｇ将斜纹夜蛾核型多角体病毒（ＳｌＭＮＰＶ）的ｐ７４基因定位于ＸｈｏⅠ４９ｋｂ、ＥｃｏＲⅠ４４ｋｂ、ＢａｍＨⅠ３０ｋｂ片段上．通过酶切图谱分析和部分序列测定,将其ｐ７４基因精确定位．用计算机软件ＤＮＡＳＩＳ和ＰＲＯＳＩＳ对这部分ＤＮＡ序列进行分析发现其与ＡｃＭＮＰＶｐ７４基因的同源性为６９％,其可能编码多肽的氨基酸顺序与ＡｃＭＮＰＶｐ７４相应部分的同源性为６６％． 相似文献
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鸡传染性法氏囊炎病毒（ＩｎｆｅｃｔｉｏｎｓＢｕｒｓａｌＤｉｓｅａｓｅＶｉｒｎｓ，ＩＢＤＶ）和禽呼肠孤病毒（ＡｖｉａｎＲｅｏｖｉ－ｒｕｓ，ＡＲＶ）的许多生物学特性和理化特性十分相似［１－４］，尤其在形态结构和理化特性上极为相似［５］，本文用ＳＤＳ—ＰＡＧＥ区别鉴定ＩＢＤＶ和ＡＲＶ是一种简便、快速、可靠的方法。 相似文献
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在测试了ＡｌＧａＡｓ／ＧａＡｓＨＢＴ（异质结晶体管）的直流特性和Ｓ参数的基础上,建立了其微波小信号等效电路,准确的等效电路有利于其微波线性应用的分析．应用Ｖｏｌｔｅｒａ级数,计算了ＡｌＧａＡｓ／ＧａＡｓＨＢＴ放大器的三阶互调失真,计算结果和双音测试结果相当一致,该ＨＢＴ良好的线性特性证明了其较好的线性应用前景 相似文献
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该文对４０例非胰岛素依赖型糖尿病（ＮＩＤＤＭ）人进行研究,就血脂ＴＧ、ＴＣ、脂蛋白ＨＤＬ－Ｃ、ＬＤＬ－Ｃ、ＶＬＤＬ－Ｃ。载脂蛋白ＡＰＯＡＩ、ＡＰＯＢ水平与２０例年龄、平均体重指数（ＢＭＩ）相当的正常健康人进行对照,测得ＮＩＤＤＭ组的ＴＧ、ＴＣ比正常对照组高,两组之间差异显著。作者检测的结果表明,用ＡＰＯＡＩ／ＡＰＯＢ和ＬＯＬ－Ｃ／ＨＤＬ－Ｃ的比值来比较,比单项测定结果的差异更显著,是判别ＮＩＤＤＭ病人动脉粥样硬化更良好的指标。 相似文献
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邱崇丙 《唐山师范学院学报》2014,(1):21-23
汉字的六书,《说文》对“转注”一类,语焉不详。后世学者提及转注,也仅限于许慎所举出的例字。《汉字例说》一文,作者从转注的角度综合考虑,对部分现代常用字作了分析,跟传统的解释有所不同。希望能抛砖引玉,互相切磋,以推进学术研究。 相似文献
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《海外英语》2007,(5):10-11
Many college freshmen arrive woefully unprepared to do college work, and as disadvantaged populations continue to grow, the share of the American work force that has made it through college is expected to plummet. Many experts blame that educational failure not just on high schools but also on colleges. School & College, a special report by The Chronicle, looks at efforts to fix the system. What reforms would better prepare students for college? What should schools and colleges be doing differently? How should state and federal officials help? 相似文献
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Rachel Zupek 《海外英语》2007,(11):40-41
我们绝大部分人都同意一件事情——我们喜欢金钱。但是你对金钱的喜欢之情足够到让你创立一份事业么?.财富通常被人忽视,主要在于人们不能真正了 相似文献
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《海外英语》2007,(4):36
There are numbers of crossroads on our long and unpredictable life journey where we totally have no idea about which direction to choose. No matter what our decision is, we should not turn back, but face the music and go ahead instead. I am this kind of girl who always does try without regretting, one example is how I dealt with my love. 相似文献
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YANGShiguo 《重庆大学学报(英文版)》2004,3(1):86-88
The problem on the geometrc inequalities involving an n-dimensional simplex and its inscribed simplex is studied. Aninequality is established, which reveals that the difference between the squared circumradius of the n-dimensional simplex andthe squared distance between its circumcenter and barycenter times the squared circumradius of its inscribed simplex is not lessthan the 2(n-1)th power of n times its squared inradius, and is equal to when the simplex is regular and its inscribed siplex is atangent point one. Deduction from this inequality reaches a generalization of n-dimensional Euler inequality indicating that thecircumradius of the simplex is not less than the n-fold inradius. Another inequality is derived to present the relationship betweenthe circumradius of the n-dimensional simplex and the circumradius and inradius of its pedal simplex. 相似文献
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Migration occurs behind a variety of reasons and has a great effect on the whole world. People may migrate in order to improve their economic situation, or in order to escape civil strife, persecution, and environmental disasters. The impact of migration is complex, bringing both benefits anddisadvantages. This paper briefly talks about the causes of migration, the allocation of benefits, and the ways in which individual countries and the international community deal with this important subject. 相似文献
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GUO Bao-feng Xiao Da-wei 《重庆大学学报(英文版)》2005,4(3):129-133
Using virtual reality for interactive design gives a designer an intuitive vision of a design and allows the designer to achieve a viable, optimal solution in a timely manner. The article discusses the process of making the Virtual Reality System of the Humble Administrator＇s Garden. Translating building data to the Virtual Reality Modeling Language （VRML） is by far unsatisfactory. This creates a challenge for computer designers to do optimization to meet requirements. Five different approaches to optimize models have been presented in this paper. The other methods are to optimize VRML and to reduce the file size. This is done by keeping polygon counts to a minimum and by applying such techniques as object culling and level-ofdetail switching. 相似文献