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1.
To examine the effects of co-culture with bone marrow mesenchymal stem cells on expansion of hematopoietic stem/progenitor
cells and the capacities of rapid neutrophil engraftment and hematopoietic reconstitution of the expanded cells, we expanded
mononuclear cells (MNCs) and CD34+/c-kit+ cells from mouse bone marrow and transplanted the expanded cells into the irradiated mice. MNCs were isolated from mouse
bone marrow and CD34+/c-kit+ cells were selected from MNCs by using MoFlo Cell Sorter. MNCs and CD34+/c-kit+ cells were co-cultured with mouse bone marrow-derived mesenchymal stem cells (MSCs) under a two-step expansion. The expanded
cells were then transplanted into sublethally irradiated BDF1 mice. Results showed that the co-culture with MSCs resulted
in expansions of median total nucleated cells, CD34+ cells, GM-CFC and HPP-CFC respectively by 10.8-, 4.8-, 65.9- and 38.8-fold for the mononuclear cell culture, and respectively
by 76.1-, 2.9-, 71.7- and 51.8-fold for the CD34+/c-kit+ cell culture. The expanded cells could rapidly engraft in the sublethally irradiated mice and reconstitute their hematopoiesis.
Co-cultures with MSCs in conjunction with two-step expansion increased expansions of total nucleated cells, GM-CFC and HPP-CFC,
which led us to conclude MSCs may create favorable environment for expansions of hematopoietic stem/progenitor cells. The
availability of increased numbers of expanded cells by the co-culture with MSCs may result in more rapid engraftment of neutrophils
following infusion to transplant recipients.
Project supported by NIH-Blood, Heart & Lung (National Institute of Health, USA, IR 01 4L70593-01) and Zhejiang Provincial
Science Foundation (No. 011103397), China 相似文献
2.
Zhi-qiang Ying Dan-dan Zhong Geng Xu Miao-yan Chen Qing-yu Chen 《Journal of Zhejiang University. Science. B》2009,10(5):368-374
Objective: To study the relationship between plasma adiponectin concentration and the functional activities of circulating endothelial progenitor cells (EPCs) in patients with coronary artery disease (CAD). Methods: Circulating EPCs were enumerated as AC133+/KDR+ cells via flow cytometry and identified by co-staining with Dii-acLDL and fluorescein isothiocy-anate (FITC)-conjugated lectin under a fluorescent microscope. The migratory capacity of EPCs was measured by modified Boyden chamber assay. Adhesion capacity was performed to count adherent cells after replating EPCs on six-well culture dishes coated with fibronectin. Results: The number of circulating EPCs (AC133+/KDR+ cells) decreased significantly in CAD patients, compared with control subjects [(74.2±12.3) vs (83.5±12.9) cells/ml blood, P<0.0\]. In addition, the number of EPCs also decreased in CAD patients after ex vivo cultivation [(54.4±8.6) vs (71.9±11.6) EPCs/field, P<0.01]. Both circulating EPCs and differentiated EPCs were positively correlated with plasma adiponectin concentration. The functional activities of EPCs from CAD patients, such as migratory and adherent capacities, were also impaired, compared with control subjects, and positively correlated with plasma adiponectin concentration. Conclusion: The study demonstrates that the impairment of the number and functional activities of EPCs in CAD patients is correlated with their lower plasma adiponectin concentrations. 相似文献
3.
Kang-ting Ji Jun-de Chai Cheng Xing Jin-liang Nan Peng-lin Yang Ji-fei Tang 《Journal of Zhejiang University. Science. B》2010,11(8):618-626
In this study,we examined the protective effects of Danshen both on endothelial progenitor cells(EPCs) in patients with hypercholesterolemia and on in-vitro EPCs of healthy volunteers.In the clinical study,we randomly divided 24 subjects with hypercholesterolemia into two groups(the control group and the Danshen-treated group).At the end of two weeks of treatment,the EPC cellular functions of both groups were tested.The results indicated that,compared to the control group,EPCs in the Danshen-treated group showed significantly better cellular functions,which was manifested in the cloning number,the proliferation capacity,the number of EPC adhesions,and cell migration.In the subsequent in-vitro experiments,EPCs were treated with vehicle,oxidized low-density lipoprotein(Ox-LDL,100 μg/ml),or Ox-LDL(100 μg/ml) plus different concentrations of Danshen(Danshensu 2,10,or 50 μg/ml,respectively) for 24 h.The results showed that Danshen treatments can prevent the detrimental effects of Ox-LDL on EPC cellular functions measured by proliferation capacity(0.24±0.08,0.37±0.11,0.30±0.04 vs.0.13±0.02,P0.05,P0.01,and P0.01,respectively),and adhesion ability(63.00±11.60,70.00±10.80,85.50±11.41 vs.40.50±6.85,all P0.01).Compared to the group treated with Ox-LDL alone,Danshen treatment significantly decreased the lipid peroxidation end product malondialdehyde(MDA) [(4.34±0.54),(3.98±0.47),(3.46±0.31) vs.(5.57±0.64) nmol/ml,all P0.01],increased the production of superoxide dismutase(SOD) [(29.74±0.71),(31.09±0.83),(30.41±0.65) vs.(14.76±3.99) U/ml,all P0.01],and lowered the expression of interleukin-6(IL-6) [(24.62±7.69),(27.04±3.14),(33.38±18.86) vs.(230.67±33.53) pg/ml,all P0.01] and tumor necrosis factor-α(TNF-α) [(41.72±6.10),(17.02±6.82),(3.73±2.26) vs.(228.71±41.53) pg/ml,all P0.01] in Ox-LDL treated EPCs.These results suggest that Danshen may exert a protective effect through its antioxidant and anti-inflammatory features. 相似文献
4.
肉鸡肺动脉高压肝脏和心脏线粒体氧自由基变化研究 总被引：6，自引：0，他引：6
400只艾维茵肉鸡按常规方法育雏在21日龄时随机分为两组,A组200只在24℃下饲养,B组200只在低温12℃下饲养。在28、35、42、49日龄测定腹水心脏指数和红细胞压积,分离鉴定腹水肉鸡心、肝线粒体,测定其所含的MnSOD活性以及线粒体膜结构的MDA含量变化经。结果表明:(1)低温处理后一周和二周,在肝脏和心脏线粒体中,SOD含量升高或显著升高(p<0.05)。MDA含量降低或显著降低(p<0.05)。(2)低温处理三周后,SOD的含量降低或显著降低。MDA的含量升高或显著升高(p<0.05)。本实验结果显示肝脏和心脏的线粒体SOD和MDA的变化与肉鸡腹水综合征的发生发展关系密切,氧自由基在亚细胞水平上参与了疾病的病理过程。 相似文献
5.
低聚壳聚糖对肉仔鸡生长性能及免疫器官发育的影响 总被引：1，自引：0，他引：1
480羽1日龄AA肉鸡,随机分为4组,每组120羽,设6个重复,其中空白对照组饲喂玉米-豆粕型基础日粮,抗生素组基础日粮添加50mg/kg金霉素,试验Ⅰ组(低聚壳聚糖剂量组)和试验Ⅱ组(黄芪多糖高剂量组)分别添加20mg/kg和40mg/kg低聚壳聚糖,试验期42d,研究低聚壳聚糖对肉仔鸡生长性能及免疫器官发育的影响。结果显示,试验Ⅰ、Ⅱ组肉仔鸡的平均体重、日增重、免疫器官重量及其指数均不同程度高于同期空白对照组和抗生素组,其中平均体重与日增重多差异显著(P<0.05),部分免疫器官重量与指数有统计学意义(P<0.05);平均料重比均较明显地低于同日龄空白对照组和抗生素组。试验结果表明,基础日粮添加20、40mg/kg低聚壳聚糖对肉鸡的生长性能及免疫器官发育具有较明显的促进作用,可提高肉鸡的平均日增重和饲料转化率,增强机体的免疫功能。 相似文献
6.
试验研究了中草药免疫促进剂对AA仔鸡法氏囊和脾脏形态结构的影响。选用1日龄健康的AA仔鸡200只,随机分为实验组和对照组,每组100只,分四个重复组。中草药免疫促进剂按200 mg/kg添加基础日粮中。在42日龄时,从每个重复组中随机抽取10只仔鸡,颈静脉放血处死,采集各组鸡法氏囊和脾脏的组织样本,置于Bouin固定液中,应用石蜡组织切片技术制作组织切片,显微摄影系统观察拍照。试验结果表明添加中草药免疫促进剂的试验组仔鸡的法氏囊和脾脏的形态结构较对照组鸡退化延缓,其中脾脏的组织结构变化比较明显;试验组鸡的法氏囊较对照组皱襞发达,法氏囊小结排列紧密;脾脏组织结构中红髓与白髓界线清晰,脾小结试验组较对照组明显增多。结果表明,中草药免疫促进剂能够促进AA仔鸡免疫器官的发育。 相似文献
7.
Conclusions In spite of very high toxic potency of PAH, in India environmental monitoring and assessment of PAH is rarely done compared
to European countries and US. The Central Pollution Control Board (CPCB) had recently initiated regular monitoring of PAHs
in ambient air in select cities. According to CPCB, ambient air PAH in Delhi ranged between 9.4–60.9 nanograms per cubic meter
(ng/m3) during 1999–2000 with higher values recorded in winter. Other research studies reported about 4.999.56 ng/m3 of benzo[a]pyrene in suspended paniculate matter (SPM) during 1986 in urban Delhi. In Calcutta, sum of 12 PAHs was 22.91 and 190.96
ng/m3 in SPM at Jadavpur and Maulali respectively, in 1994. The average benzo[a]pyrene concentration was 10.4+4.76 ng/m3. These levels are apparently manifold higher than the European standards. Apart from few such studies no detailed studies
are available in India. Even in the current standards and specifications for diesel engines there is no emission limit for
PAH. Recently it had specified that 10% of PAH by hydrocarbons as limit that should be adopted by 2005.
In the current Indian scenario, especially after the economic liberalization in 1991, the motor vehicle population is increasing
rapidly, leading to corresponding increase in PAH emissions. The role of PAHs in cardiovascular mortality, cancer cases and
several other health abnormalities that are reported nowadays cannot be ignored. 相似文献
8.
为研究不同营养水平日粮对早期饲喂的肉鸡生产性能和免疫机能的影响,选择刚出壳AA肉仔鸡480只（雌雄各半）,对照组饲喂常规日粮,试验Ⅰ组饲喂高营养水平日粮,试验Ⅱ组饲喂低营养水平日粮．结果表明：试验Ⅰ组体重分别比对照组和试验Ⅱ组提高5．28％（P〈0．05）和9．61％（P〈0．05）;法氏囊指数分别比对照组和试验Ⅱ组提高6．27％（P〈0．05）和9．75％（P〈0．05）,脾脏指数分别提高5．8％（P〈0．05）和13．84％（P〈0．05）;试验Ⅰ组新城疫抗体水平分别提高2．92％和12．5％（P〈0．05）．高营养水平日粮有利于生产性能和免疫机能的提高． 相似文献
9.
选择150只1日龄AA健康商品鸡,随机分成3组,每组50只.研究日粮添加免疫调节剂C96对肉鸡增重和屠宰主要指标的影响.结果表明:日粮添加适量的免疫调节剂C96可以促进肉仔鸡生长发育,提高屠宰性能. 相似文献
10.
This paper aims to screen and identify sphere clone cells with characteristics similar to cancer stem cells in human gallbladder
cancer cell line GBC-SD. GBC-SD cells were cultured in a serum-free culture medium with different concentrations of the chemotherapeutic
drug cisplatin for generating sphere clones. The mRNA expressions of stem cell-related genes CD133, OCT-4, Nanog, and drug
resistance genes ABCG2 and MDR-1 in sphere clones were detected by quantitative real-time polymerase chain reaction (PCR).
Stem cell markers were also analyzed by flow cytometry and immunofluorescent staining. Different amounts of sphere clones
were injected into nude mice to test their abilities to form tumors. Sphere clones were formed in serum-free culture medium
containing cisplatin (30 μmol/L). Flow cytometry results demonstrated that the sphere clones expressed high levels of stem
cell markers CD133+ (97.6%) and CD44+ (77.9%) and low levels of CD24+ (2.3%). These clones also overexpressed the drug resistance genes ABCG2 and MDR-1. Quantitative real-time PCR showed that
sphere clones expressed stem cell genes Nanog and OCT-4 284 and 266 times, respectively, more than those in the original GBC-SD
cells. Immunofluorescent staining showed that sphere clones overexpressed OCT-4, Nanog, and SOX-2, and low expressed MUC1
and vimentin. Tumor formation experiments showed that 1×103 sphere clone cells could induce much larger tumors in nude mice than 1×105 GBC-SD cells. In conclusion, sphere clones of gallbladder cancer with stem cell-like characteristics can be obtained using
suspension cultures of GBC-SD cells in serum-free culture medium containing cisplatin. 相似文献
11.
Hao Lu Wen-zhi Pan Quan Wan Lei-lei Cheng Xian-hong Shu Cui-zhen Pan Ju-ying Qian Jun-bo Ge 《Journal of Zhejiang University. Science. B》2016,17(1):54-59
There is a paucity of data regarding trends in the incidence of heart disease in China during recent years. Using a large echocardiography database in our center, we analyzed trends in the prevalence of several common heart diseases from Dec. 2003. This study retrospectively analyzed the echocardiographic database in our Department from 2003 to 2012. A total of 385 682 cases were included in the study. The prevalence of rheumatic heart disease decreased over the 10-year period, from 4.04% in 2003 to 3.06% in 2012 (P<0.01). Infective endocarditis also decreased, from a mean prevalence of 0.37% in July 2003 to 0.27% in Dec. 2008 (P<0.001). The prevalence of hypertrophic cardiomyopathy, which includes 20% apical hypertrophic cardiomyopathy and 20% hypertrophic obstructive cardiomyopathy, was about 1.8%. The prevalence of the three most common adult congenital heart diseases (CHDs) decreased by about 10% from July 2003 to Dec. 2008 (all P<0.001). The prevalence of moderate pulmonary arterial hypertension (PAH) or left ventricular systolic dysfunction (LVSD) decreased during the 10-year period (P<0.001), but there was no change in the prevalence of severe PAH or LVSD (P>0.05). The present study indirectly demonstrates that the prevalence of several common heart diseases in China has declined in recent years. 相似文献
12.
试验研究了四黄提取物对AA肉鸡法氏囊组织结构的影响。选用1日龄健康的AA肉仔鸡200只,随机分为实验组和对照组,每组100只,分四个重复组。四黄提取物按200 mg/kg添加基础日粮中。试验期为42天。在42日龄时,从每个重复组中随机抽取10只鸡,颈静脉放血处死,采集各组鸡法氏囊的组织样本,置于Bouin固定液中,应用石蜡组织切片技术制作组织切片,OlympusCH-30显微摄影系统观察拍照。试验结果表明添加四黄提取物的试验组鸡的法氏囊较对照组鸡退化延缓,其中试验组鸡的法氏囊较对照组皱襞发达,法氏囊淋巴小结排列紧密。结果表明,四黄提取物可以延缓AA肉仔鸡法氏囊的退化。 相似文献
13.
本研究采用数量限饲,探讨不同限饲起始日龄对肉鸡整个生产周期中的福利状况和免疫器官发育的影响.[方法]选1日龄AA肉用仔鸡360只,随机分为3个处理,每个处理设3个重复,每个重复40只.Ⅰ组为对照组,Ⅱ、Ⅲ组的限饲开始日龄分别为8日龄和12日龄,限饲组限饲时间为7d,限饲强度均为对照组前一天采食量的80％,限饲组在非限饲期与对照组肉仔鸡均为自有采食和饮水.[结果]早期限饲可显著降低35日龄、42日龄肉鸡胫骨长、尺骨长不对称性综合FA（P ＜0.05）;42日龄时肉鸡足底和腿关节的重度、中度和轻度灼伤各组之间差异不显著（P＞0.05）,但无灼伤率两试验组与对照组差异显著（P＜ 0.05）;42日龄时肉鸡的腿肌率、胸肌率两试验组与对照组差异季显著（P＜0.01）.早期限饲显著提高了肉鸡28日龄的法氏囊指数（P＜0.05）,8-14日龄限饲对28日龄时的脾脏指数影响显著（P＜0.05）;各处理组在14日龄、21日龄、28日龄、35日龄和42日龄时NDV抗体滴度差异不显著（P＞0.05）,但在28日龄后的血液中ND的抗体滴度增幅,试验Ⅱ、Ⅲ组高于对照组.[结论]8-14日龄限饲可有效改善肉鸡的福利状况,促进了免疫器官的发育,提升了饲养后期肉鸡血清中的ND抗体滴度. 相似文献
14.
为研究早期饲喂对肉鸡生长性能和肠道消化酶的影响,选择360只刚出壳的AA肉用雏鸡,采用单因子试验设计,随机分成4个处理组,每组4个重复,每个重复20只,试验1组出壳后12 h饲喂,试验Ⅱ组出壳24 h后饲喂,试验Ⅲ组出壳36 h后饲喂,对照组出壳48 h后饲喂.饲喂相同日粮,试验期为6周.于21日龄和42日龄分别测定肉鸡进行生产性能和酶活性.结果表明：早期饲喂显著提高了肉鸡的日增重,降低了料重比,试验Ⅰ组和试验Ⅱ组与对照组相比,日增重分别提高了4.19%和3.70%,料重比分别降低5.61%和5.10%,差异显著（P〈0.05）;试验Ⅰ组、试验Ⅱ组和试验Ⅲ组肠道消化酶水平高于对照组.早期饲喂提高了肉鸡肠道消化酶水平,以出壳后12 h饲喂最好. 相似文献
15.
INTRODUCTION Pulmonary hypertension is not rare in humanwith some heart and lung diseases. Chronic pul-monary hypertension leads to structural alterationsof the lung vessels. The pathophysiology of thisremodeling process is still poorly understood.Furthermore, the structural damage of the lungvessels limits the clinical success of vasodilatortreatment. Assuming genetic susceptibility, shearstress and inflammation are the principal pathoge-netic factors involved in lung vessel remode… 相似文献
16.
Objective: To investigate the evolution of pulmonary hypertension, the pathological changes of pulmonaryarteries, and the expression of VEGFmRNA and eNOSmRNA of pulmonary arterial endothelial cells in immature rabbitstreated with intratracheal bleomycin (BLM). Methods: Immature rabbits were divided into control and BLM group. Twoand four weeks after intratracheal normal saline or BLM injection, the systolic, diastolic and mean pulmonary arterypressure (PASP, PADP, MPAP) were measured by micro-catheter; the pathological changes and the expression ofVEGFmRNA and eNOSmRNA of endothelial cells in pulmonary arteries were evaluated by HE and in situ hybridization.Results: Two and four weeks after intratracheal injection of BLM, the PASP, PADP and MPAP increased 53%, 49%, 52%in 2 weeks, and 43%, 89%, 56% in 4 weeks; the wall thickness increased and the cavity in middle and small pulmonaryarteries became narrow; the Thickness Index (TI) and Area Index (AI) increased 25%, 14% in 2 weeks, and 22%, 24% in 4weeks; the level of VEGFmRNA and eNOSmRNA expression decreased 46%, 43% in 2 weeks, and 43%, 51% in 4 weeks.There was no significant difference between 2 weeks and 4 weeks BLM groups. Conclusion: The pulmonary artery pressurewas elevated, the thickness of wall increased and the cavity became narrow in middle and small pulmonary arteries, and thelevel of VEGFmRNA and eNOSmRNA expression in pulmonary arterial endothelial cells decreased in immature rabbitsafter 2 weeks and 4 weeks of intratracheal 4 U/kg BLM injection. 相似文献
17.
本试验随机选取了在相同条件下饲养的120日龄贵妃鸡、怀乡鸡和北京油鸡三种优质鸡母鸡各24只,分别取其胸肌和腿肌做成切片,用于对三种优质鸡的母鸡肌纤维密度、肌纤维长度和肉品质进行比较,同时对肌纤维特性与肉品质的相关性进行研究,结果表明:(1)肌纤维特性方面:三种优质鸡腿肌直径大于胸肌直径,相差范围在0.73～2.6μm之间,其中怀乡鸡>北京油鸡>贵妃鸡;三种优质鸡胸肌密度大于腿肌密度,相差范围在43.18～181.56根/mm2之间,其中北京油鸡>怀乡鸡>贵妃鸡.(2)肉品质方面:三种优质鸡腿肌肉色大于胸肌肉色,差异极显著(P<0.01),相差范围在2.62～9.01之间,其中北京油鸡胸肌肉色大于怀乡鸡,而怀乡鸡胸肌肉色大于贵妃鸡,分别大1.33和3.2,腿肌肉色上,北京油鸡大于贵妃鸡,贵妃鸡大于怀乡鸡,分别大3.64和3.19;三种优质鸡胸鸡和腿肌pH值差异不显著(P>0.05),范围在6.1～6.2之间;三种优质鸡胸肌滴水损失大于腿肌,差异极显著(P<0.01),其中胸肌上,贵妃鸡滴水损失大于怀乡鸡,怀乡鸡大于北京油鸡,分别大0.82和3.09,腿肌滴水损失上,怀乡鸡滴水损失大于贵妃鸡,贵妃鸡大于北京油鸡,分别大2.3和3.49.(3)相关性方面:总体上看,肌纤维直径与密度呈负相关关系(r<0),肌纤维特性与肉色呈正相关关系(r>1),肌纤维特性与肌肉pH值和滴水损失呈负相关关系(r<0). 相似文献
18.
IBD自1984年在我省流行以来,疫情不断,损失惨重,各地使用各种IBD弱毒苗免疫鸡群,免疫效果欠佳,笔者研制IBD油乳剂组织灭活苗,在一定范围内免疫鸡群,可达91％的保护率。 相似文献
19.
Background:The adhesion of monocytes to the endothelium following accumulation oflow-density lipoprotein(LDL) in subendothelial spaces is an important step in the development of intimal hyperplasia in arterially implanted vein grafts and atherosclerosis in both animals and humans.However.it iS not well known how serum factors affect the adhesion of monocytes.Methods:We have studied the efrect of fetal calf serum(FCS).which we considered a source of LDL.on the adhesion of monocytes to endothelial cells(Ecs)by using human monocytic THP-1 cells and both a monolayer of cultured bovine aortic endothelial cells(EC monoculture)and a co-culture with bovine aortic smooth muscle cells(EC-SMC co-culture).Results:It was found that the addition of FCS to the medium greatly affected the adhesion of THP-1 cells,and the higher the concentration of FCS in the medium,the greater the adhesion of THP-1 cells to endothelial cells.Adhesion of THP-1 cells to an EC-SMC co-culture Was approximately twofold greater than that to an EC monoculture,and after adhering to endothelial cells,many THP-1 cells transmigrated into the layer of smooth muscle cells.Conclusion:The results suggest that the elevation of the LDL(cholesterol)level in blood provides a favorable condition for the development of intimal hyperplasia and atherosclerosis by promoting the adhesion of monocytcs to the endothelium and their subsequent migration into subendothelial spaces. 相似文献
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谈即时定制生产模式生产能力实现机制 总被引：1，自引：0，他引：1
文章分析了即时定制环境下企业生产能力的特点及其系统构成要素，分别从企业战略层面上和战术层面上研究了即时定制环境下企业生产能力的特点和实现机制。由于生产能力绩效衡量指标体系的建立是优化IC企业生产能力的前提和基础，因此本文提出了即时定制生产模式下企业生产能力绩效衡量指标及其指标体系。 相似文献