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目的:观察大鼠急性脊髓损伤后GFAP在受损脊髓的不同时间点的表达变化,分析GFAP在脊髓内源性神经干细胞的增殖和分化过程中的作用机制,明确川芎嗪治疗脊髓损伤的分子机制。方法:将SD大鼠随机分组后利用改良Allen's法建立大鼠急性脊髓损伤模型。于造模后第8h、1d、3d、7d、14d、28d每组分别处死6只大鼠取材,免疫组化检测Brdu+、Nestin+细胞的表达及GFAP表达变化情况,并对结果进行统计分析和相关性分析。结果:脊髓损伤后,Brdu+细胞被激活,在脊髓中的表达明显增多,在脊髓白质、灰质及中央管室管膜区均有表达。Brdu+、Nestin+、GFAP+细胞表达均在第7天达到峰值,随后逐渐减少,且在损伤后第7天、14天、28天,川芎嗪组与C组比较均有显著差异(P0.01)。经对GFAP、Nestin+和Brdu+细胞在脊髓中的表达数进行相关性分析,GFAP表达与Nestin+细胞表达呈正相关趋势。结论:(1)川芎嗪能够促进脊髓损伤后GFAP的表达;(2)川芎嗪能够对脊髓损伤后内源性神经干细胞增殖分化有促进作用;(3)脊髓损伤早期GFAP的表达与脊髓内源性干细胞的增殖分化呈正相关趋势。 相似文献
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《内蒙古科技与经济》2015,(20)
目的:建立一种操作简单、可重复、稳定的完全急性脊髓损伤(ASCI)动物模型,并对动物神经功能恢复进行初步评价。方法:将27只雌性小鼠随机分为3组(A/B/C),每组9只,钳夹法构建小鼠T9-T11节段ASCI模型,A组为钳夹2秒,B组为钳夹5秒,C组为钳夹10秒,采用改良组织学与行为学方法对模型动物脊髓损伤后神经功能恢复进行评价。结果:小鼠胸椎T9-T1节段急性钳夹损伤后,损伤脊髓处出现明显的出血瘢痕,后组织水肿,髓鞘变性,损伤处上下游出现空泡继而形成瘢痕等一系列的病理变化,3组比较在术后后肢运动功能BMS评分上有统计学差异(p0.05)。3组术后组织学评分在脊髓损伤程度上有统计学差异(p0.05)。结论:成功建立稳定性好、重复性强且具有治疗意义的小鼠脊髓钳夹损伤模型;(钳夹5秒组)具备较好的实验价值。 相似文献
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急性肺损伤是全身炎症反应综合征在肺部的主要表现,往往也是多器官功能障碍综合征中最先出现的器官功能障碍,在其整个发病过程中居重要甚至是决定性的地位.合理地建立理想的急性肺损伤的动物模型对研究急性肺损伤的发病机制及早期防治具有重要意义. 相似文献
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目前国内外已建设的人类疾病动物模型资源数据库尚未涵盖所有疾病,也无统一的资源查询路径或渠道,为此,探索开发一个较为完整和可以资源共享的人类疾病动物模型数据平台。收集和整理挖掘相关文献、专著、研究机构的人类疾病动物模型资源数据,以促进人类疾病动物模型资源数据整合、挖掘和共享为目标,从系统疾病、动物种属、造模方法3个维度搭建人类疾病动物模型资源共享平台总体架构,在全面性、可扩展性、开放性、稳定性和安全性的设计原则指导下,采用当前主流的B/S结构,引入可扩展标记语言对异构数据进行结构化和标准化处理,再以层次结构法对资源数据进行分类和编码,最后经专家审核通过发布。该平台目前试运行结果显示,既便于用户查找符合需求的数据,同时也保证数据的准确性。 相似文献
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正引言确定结构和机械疲劳寿命的方法主要有两类:试验法和分析法。确定疲劳寿命的分析法是依据材料的疲劳性能,对照结构所受到的载荷历程,按分析模型来确定结构的疲劳寿命。疲劳寿命分析方法一般包含有三部分的内容:1)材料疲劳行为的描述;2)循环载荷下结构的响应;3)疲劳累积损伤法则。如图1。在民用飞机结构疲劳分析中,一般采用应力疲劳分析方法。应力疲劳分析方法包含了材料疲劳性能、载荷谱、疲劳累积损伤三个要素。DFR——Detail Fatigue Rating疲劳分析方法,即细节疲劳额定强度分析方法,是在民用飞机结构疲劳分析方法中的一种常见分析方法。本文将对DFR疲劳分析方法的过程进行分析,并对其中的几个参数进行讨论。 相似文献
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1排球运动员膝关节损伤机理排球运动员的滑步防守、急停等动作都要求膝关节处于半蹲位130°-135°角的位置上发力。这就使髌、股关节面之间接触范围及髌骨软骨面承受的压力最大,关节面之间反复撞击、挤压与摩擦,长期作用下会使软骨细胞被挤压变性或 相似文献
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传统的斜拉桥滑坡成因机制及稳定性分析方法斜拉桥数据关系模糊,导致分析准确度较差、数据集成度低的缺陷,为了解决上述问题,引入位移反分析法对斜拉桥滑坡成因机制及稳定性分析方法进行研究。根据斜拉桥的建设环境,对斜拉桥滑坡的成因机制进行分析,以此为基础,根据实际情况构建斜拉桥模型,以模型为工具采用位移反分析法对斜拉桥受力情况进行分析,通过勾股定理搭建斜拉桥数据关系,以搭建的斜拉桥数据关系为依据,基于极限平衡法计算斜拉桥滑坡稳定性系数,实现了斜拉桥滑坡成因机制及稳定性的分析。通过实验得到,与传统的斜拉桥滑坡成因机制及稳定性分析方法相比较,提出的斜拉桥滑坡成因机制及稳定性分析方法极大的提升了分析准确度与数据集成度,充分说明提出的斜拉桥滑坡成因机制及稳定性分析方法具备更好的分析效果。 相似文献
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目的:研究运过度运动导致的导致急性心内膜炎预防与治疗方法,预防运动性心肌损伤发射。方法:采用PAFAFAC分析方法结合多元线性回归分别对两组近红外光谱数据进行了分析,得到人体三层骨骼肌模型的蒙特卡罗模拟,由广义梯度近似GGA观测免疫细胞凋亡模型,通过对血液样本中的羧甲基纤维素锂(CMC-S)进行合成流变分析,分析患者在躯体神经支配下心肌内膜收缩或舒张。结果:通过运动骨骼肌免疫细胞凋亡抑制方法,能有效对过度运动训练损伤导致的急性心内膜炎等并发症实现诊疗。肺动脉收缩压有82.6 mm Hg改善为92.0 mm Hg,肺动脉舒张压有44.9 mm Hg改善为50.3mm Hg。结论:采用免疫细胞凋亡干预后,肺动脉能短期实现修复,可以改善患者的脉氧饱和度,合并胸痛、浮肿、高烧等急性心内膜炎病症,从而避免运动员出现急性心内膜炎的症状,对避免运动员因过度训练导致的心衰竭和体循环缺血缺氧等也有积极的临床疗效。 相似文献
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《中国科学院院刊(英文版)》2008,22(1):2-3
CAS should stick to the principle of rendering service to, and giving impetus for, the development of China＇s science enterprise by making S＆T innovations, said CAS President LU Yongxiang. The CAS president made the remarks in a recent talk to communicate the gist of the winter session of the Party＇s Leading-member Group at CAS, which was held from 7 to 11 January in Beijing. 相似文献
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《中国科学院院刊(英文版)》2008,22(1)
With great care, Dr. ZHOU Zhonghe takes out a package wrapped by cotton tissue from a drawer and says: "This is the gem of our collections: the fossil of a bird that lived 125 million years ago!" Then, pointing at a tiny mound, he explains: "Look, this is the claw and that is the head. It was in the egg shell and ready to hatch ... The species fell into a family of waterside inhabitants." 相似文献
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Prof. SUN Changpu from the CAS Institute of Theoretical Physics coworkers from University of Basel in Switzerland have worked out a way - at least in theory - to split a beam of molecules according to their chirality. The technique involves passing the molecules through three different laser beams is similar to the famous Stern-Gerlach effect whereby a beam of atoms passing through a magnetic field is split in two according to the atoms' spin states 《中国科学院院刊(英文版)》2008,22(1):23-23
Prof. SUN Changpu from the CAS Institute of Theoretical Physics and coworkers from University of Basel in Switzerland have worked out a way --at least in theory --to split a beam of molecules according to their chirality. The technique involves passing the molecules through three different laser beams and is similar to the famous Stern-Gerlach effect, whereby a beam of atoms passing through a magnetic field is split in two according to the atoms＇ spin states （Phys. Rev. Lett. 99 130403）. 相似文献
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《中国科学院院刊(英文版)》2014,(3):212-213
<正>In February 2014,the 30th Chinese Antarctic inland expedition team reported that they have successfully conducted the seeing measurement at the Antarctic Taishan Station using a Differential Image Motion Monitor(DIMM)developed by the Nanjing Institute of Astronomical Optic and Technology(NIAOT),Chinese Academy of Sciences.In astronomy,seeing is a physical parameter that describes the irregular movement and blur of the star 相似文献
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XIN Ling 《中国科学院院刊(英文版)》2014,(4):270-271
Amassive overhaul is coming to the Chinese Academy of Sciences （CAS）. To straighten its ＂fragmented, inefficient research＂, the academy has decided to restructure its 104 institutes for better teamwork. 相似文献
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SONG Jianlan 《中国科学院院刊(英文版)》2014,(4)
<正>DNA demethylation in mammalian zygotes,a biochemical process through which the parental genomes let go the epigenetic marks from their"past lives"to obtain totipotency,has long fascinated biologists:how does it happen?A joint team at the CAS Shanghai Institutes for Biological Sciences tells a surprising story. 相似文献
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China is one of the fastest growing economies worldwide. Are science and research also in the fast lane? We talked with LUO Yuan, scientist at the Institute of Coal Chemistry in Shanxi, and Axel Mosig, Professor at Ruhr University in Bochum, who has first-hand experience working and living in China. 相似文献
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Joachim Trümper 《中国科学院院刊(英文版)》2014,(2):201-201
<正>"In 1979 two Chinese scientists joined our institute in the framework of the historical CAS-MPG agreement.This marked the beginning of a longstanding fruitful collaboration.Many visitors followed and contributed to our scientific programs.–My first visit to China took place in 1981–with stays at CAS institutes in Bejing,Nanjing,Shanghai and Kunming,where I received unforgettable impressions of 相似文献
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《中国科学院院刊(英文版)》2014,(3)
<正>May 15,2014Publicly funded scientific research is an important means for the society to create knowledge,support innovation,and promote growth.Research articles from such research are knowledge resources to be shared by all.Their open access will facilitate the dissemination and utilization of knowledge,turn the knowledge produced by public investment effectively into innovation and development capabilities of the society,ensure that public knowledge benefi ts all,and strengthen the innovation driven development strategy.Therefore,Chinese Academy of Sciences(CAS)issues at the present stage the following open access policy for articles from publicly funded scientifi c research projects:CAS requires its researchers and graduate students to 相似文献
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《中国科学院院刊(英文版)》2014,(3)
<正>Between February and March of 2013,the source of avian-origin H7N9 human infection was discovered in Shanghai and Anhui,China.Prior to this,H7N9 has only exhibited characteristics of a low pathogenic avian infl uenza virus,carried by chickens with no known cross-species transmission.By the end of August 2013,Chinese mainland reported a total of 134 confi rmed cases of H7N9 human infections across12 provinces and 42 cities,resulting in 45 deaths.In October and November,Zhejiang Province found three new cases and Guangdong found one case of human infection,suggesting the possibility of a comeback in the fall and winter seasons.Researchers led by Prof.GAO Fu(George Fu Gao)at the Institute of Microbiology,CAS and Beijing Institutes of Life Science,have made progress on understanding 相似文献