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主要论述了电化教学、AutoCAD2004、SoildWorks 2004在《机械制图》课程教学中的应用,极大地激发了学生的学习兴趣,降低了教学难度,减轻了学生的学习负担,增强了学生学习的积极性,使教学效果有了明显提高. 相似文献
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手术室等候间的建立降低了手术患者等候期间的安全隐患,缩短了手术台衔接时间,提高了手术间的利用率,同时减轻了患者的紧张恐惧心理,增进了护患关系,提升了服务质量。本文主要探讨了手术室等候间的创建与管理。 相似文献
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介绍了元托帽沟泥石流灾害现状并分析了形成的原因,对泥石流治理工程设计的水文参数进行了讨论,提出了灾害防治的措施。着重介绍了城市小流域黄土泥石流灾害防治的特点。 相似文献
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本研究成果的完成,在一定程度上解决了国资委成立后国有资产管理改革缺乏系统理论支持,理论研究与改革实践严重脱节的问题;澄清了新的历史时期在国有资产管理改革理论和实践中存在的模糊认识,改变了国有资产管理改革研究分散、研究范围狭窄的现状,突破了"为改革而改革"的局限性,整合了已有的研究成果,给出了国有资产管理理论研究和实践的基本框架和操作思路,论证了国有资产管理改革方向和目标,阐述了国有管理的组织架构与职责分工,比较了不同管理模式的特点,归纳了国有资产管理改革实践中出现的问题。 相似文献
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信息技术的发展,推动了Moodle的产生,Moodle在机械制图中的应用,让学生在学习上有了互动交流,拓展了学生的三维立体想象力,不仅提高了学生的学习兴趣,而且提高了学生的学习效率,为学生以后的工作生活奠定了基础。 相似文献
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解决了Clever算法在Web超链接结构研究方面的一个问题：Clever算法在多重连续的超链接情况下忽略了用户的浏览行为,本文引入了可行性矩阵,提出了一种改进算法,同时应用Warshll算法解决了算法复杂度问题。 相似文献
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本文阐述了客户忠诚的内涵,并对客户忠诚进行了经济学分析,指出了电子商务环境下客户忠诚的特点,找出了影响客户忠诚的因素,最后对电子商务环境下建立和巩固客户忠诚提出了解决办法. 相似文献
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Aniket V. Mali Sunita S. Bhise Surendra S. Katyare Mahabaleshwar V. Hegde 《Indian journal of clinical biochemistry : IJCB》2018,33(1):38-45
Recent studies have been noted that the erythrocytes from Type II diabetic patients show significantly altered structural and functional characteristics along with the changed intracellular concentrations of glycolytic intermediates. More recent studies from our laboratory have shown that the activities of enzymes of glycolytic pathway changed significantly in RBCs from Type II diabetic patients. In particular the levels of lactate dehydrogenase (LDH) increased significantly. Lactic acidosis is an established feature of diabetes and LDH plays a crucial role in conversion of pyruvate to lactate and reportedly, the levels of lactate are significantly high which is consistent with our observation on increased levels of LDH. Owing to this background, we examined the role of erythrocyte LDH in lactic acidosis by studying its kinetics properties in Type II diabetic patients. Km, Vmax and apparent catalytic efficiency were determined using pyruvate and NADH as the substrates. With pyruvate as the substrate the Km values were comparable but Vmax increased significantly in the diabetic group. With NADH as the substrate the enzyme activity of the diabetic group resolved in two components as against a single component in the controls. The Apparent Kcat and Kcat/Km values for pyruvate increased in the diabetic group. The Ki for pyruvate increased by two fold for the enzyme from diabetic group with a marginal decrease in Ki for NADH. The observed changes in catalytic attributes are conducive to enable the enzyme to carry the reaction in forward direction towards conversion of pyruvate to lactate leading to lactic acidosis. 相似文献
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The rate of glucose consumption and pyruvate and lactate production in red blood cells of normal, sickle cell trait and SS disease subjects were measured. Glucose consumption was found to be increased significantly (p<0.05) in red cells of sickle cell patients than the sickle cell trait and normal persons. There was also an increased rate (p<0.05) of pyruvate formation and a significant (p<0.05) increase in lactate formation in sickle red cells. 相似文献
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S. K. Bansal H. N. Pandey A. Bansal L. D. Joshi 《Indian journal of clinical biochemistry : IJCB》1992,7(2):179-184
Circadian rhythm of serum glucose, pyruvate, lactate and lactate dehydrogenase (LDH) was studied in normal healthy young volunteers
at the onset (I Group, 7 cases) and peak (II Group, 8 cases) of winters in India with highly varied temperature. There was
a significant difference, in the circadian rhythm of glucose, pyruvate and lactate dehydrogenase (LDH) of the two groups.
A clockwise shift was observed in acrophase except in serum lactate, which is related to the energy demand in association
with time qualified changes in diurnal activity of the individuals and change in photoperiod. 相似文献
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Cataract is one of the leading causes of visual disability often leading to blindness in the elderly population. One of the
causes is oxidation of proteins present in lens, by hydrogen peroxide (H2O2). In the present study 100 goat lenses were analyzed to determine the protective efficacy of ketoacids, against the oxidative
insult by H2O2. The ketoacids used were (pyruvate, alpha ketoglutarate and oxaloacetate), that are constantly produced endogenously. The
lenses were incubated as control and experimental groups in TC-199 media for 72 hrs. H2O2 concentration of 10mM was used to induce cataract. The biochemical parameters measured were levels of malondialdehyde (MDA),
a lipid peroxidation product and activity of glutathione peroxidase (G-Px), an enzymatic antioxidant. The results showed a
significant increase in the levels of MDA and significant decrease in the activity of G-Px in the cataractous lenses as compared
to control. After addition of ketoacids (pyruvate (10mM), alpha ketoglutarate (20mM) and oxaloacetate (20mM)) separately,
the levels of MDA decreased significantly and the activity of G-Px increased significantly. The results suggest that the ketoacids
can be very promising antioxidants for the treatment of cataract. They may also be useful in treating other disabilities related
to acute and chronic oxidative stress. 相似文献
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Sheshadri Narayanan 《Indian journal of clinical biochemistry : IJCB》2000,15(1):78-82
Structural similarity between a viral protein and a self-component can trigger an autoimmune response, which is the basis of molecular mimicry. Alternatively an invading virus can induce an inflammatory response which in turn can initiate an attack by hitherto dormant T cells on a specific self-antigen, a phenomenon which is referred to as Bystander Activation. Several viruses share amino acid sequences with target self-proteins. A widely studied viral interaction is the structural mimicry of a small portion of coxsackie virus to a specific region of the enzyme glutamic acid decarboxylase (GAD) which is expressed by the β cells of the islet of Langerhans in the pancreas leading to the destruction of insulin producing cells and the onset of Type I insulin dependent diabetes mellitus (IDDM). Knowledge of specific epitopes in GAD susceptible to autoimmune attack can permit devising therapeutic strategies for the prevention and suppression of IDDM. 相似文献
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BackgroundGABA (γ-aminobutyric acid) is a four-carbon nonprotein amino acid that has hypotensive, diuretic, and tranquilizing properties. Glutamate decarboxylase (GAD) is the key enzyme to generate GABA. A simple and economical method of preparing and immobilizing GAD would be helpful for GABA production. In this study, the GAD from Lactobacillus fermentum YS2 was expressed under the control of a stress-inducible promoter and was purified and immobilized in a fusion form, and its reusability was investigated.ResultsThe fusion protein CBM-GAD was expressed in Escherichia coli DH5α carrying pCROCB-gadB, which contained promoter PrpoS, cbm3 (family 3 carbohydrate-binding module from Clostridium thermocellum) coding sequence, the gadB gene from L. fermentum YS2 coding for GAD, and the T7 terminator. After a one-step purification of CBM-GAD using regenerated amorphous cellulose (RAC) as an adsorbent, SDS-PAGE analysis revealed a clear band of 71 kDa; the specific activity of the purified fusion protein CBM-GAD reached 83.6 ± 0.7 U·mg-1. After adsorption onto RAC, the immobilized GAD with CBM3 tag was repeatedly used for GABA synthesis. The protein-binding capacity of RAC was 174 ± 8 mg·g-1. The immobilized CBM-GAD could repeatedly catalyze GABA synthesis, and 8% of the initial activities was retained after 10 uses. We tested the conversion of monosodium glutamate to GABA by the immobilized enzyme; the yield reached 5.15 g/L and the productivity reached 3.09 g/L·h.ConclusionsRAC could be used as an adsorbent in one-step purification and immobilization of CBM-GAD, and the immobilized enzyme could be repeatedly used to catalyze the conversion of glutamate to GABA. 相似文献
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赵国柱 《科技成果管理与研究》2009,(10):40-41
电有机合成是用电化学方法进行有机化合物合成的科学,具有悠久的历史。它是一门涉及电化学、有机合成及化学工程等内容的边缘学科。电化学有机合成是一种新的有机合成技术。本文归纳了电化学有机合成的特点发展及现状,并介绍了电化学有机合成在石油化工中的应用情况。 相似文献
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G. Sharmila Banu G. Kumar A. G. Murugesan 《Indian journal of clinical biochemistry : IJCB》2009,24(4):414-418
The aim of this study was to investigate the ethanolic leaf extract of Trianthema portulacastrum L. (Family: Aizoaceae) on
aflatoxin induced hepatic damage in rats. Aflatoxin intoxication in rats significantly (p < 0.001) elevated the levels of
serum glutamate pyruvate transaminase (SGPT), serum glutamate oxaloacetate transaminase (SGOT), alkaline phosphatase (ALP),
and total bilirubin, which indicated acute hepatocellular damage and biliary obstruction. Ethanolic leaf extract of T. portulacastrum
showed dose dependent decrease in the levels of SGPT, SGOT, ALP and total bilirubin. Minimum effective dose of extract was
found to be 100 mg/kg body weight. Results obtained from histopathological studies also supported hepatoprotective activity
against aflatoxin-induced hepatotoxicity. Thus the study demonstrates that T. portulacastrum possess antihepatotoxic effect
against aflatoxin. 相似文献
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Santosh Kumar Singh Prashant Kumar Rai Shikha Mehta Rakesh Kumar Singh Geeta Watal 《Indian journal of clinical biochemistry : IJCB》2009,24(4):410-413
The aim of the study was to ascertain the role of ethanolic extract of Cynodon dactylon against hepatic complications in streptozotocin
(STZ) induced type 2 diabetic models. Effect of the pre identified most effective dose of 500 mg/kg body weight was studied
on hepatic injury caused by chemically induced diabetes by 55 mg/kg body weight i.p. injection of STZ in male Wistar rats.
The dose of 500mg/kg body weight given once daily for 14 days reduced the levels of serum glutamate oxaloacetate transaminase,
serum glutamate pyruvate transaminase, alkaline phosphatase, creatinine and urine sugar significantly (P<0.05) with increase
in total protein, haemoglobin and body weight was increased. High LD50 validates its high margin of safety. 相似文献
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BackgroundOrnithine decarboxylase antizyme 1 (OAZ1) is an important regulator of polyamine synthesis and uptake. Our previous studies indicated that high OAZ1 expression in the ovaries of laying geese is responsible for poor egg production. In the present study, the molecular characterization of goose OAZ1 gene was analyzed, as well as the expression profile in various follicular tissues.ResultsAn 873-bp cDNA sequence of the OAZ1 gene (Accession No. KC845302) with a + 1 frameshift site (+ 175T) was obtained. The sequence consisted of a 652-bp two overlapping open reading frames (a putative protein with 216 amino acids). The OAZ domain, OAZ signature and OAZ super family domain were prominent conserved regions among species. As the follicle size increased, OAZ1 abundance showed an increasing trend during follicular development, while it decreased during follicular regression. The level of OAZ1 mRNA expression was the lowest in the fifth largest preovulatory follicle, and was 0.65-fold compared to the small white follicle (P < 0.05). OAZ1 mRNA expression in the largest preovulatory and postovulatory follicle was 2.11- and 2.49-fold compared to the small white follicle, respectively (P < 0.05).ConclusionsThe goose OAZ1 structure confirms that OAZ1 plays an important role in ornithine decarboxylase-mediated regulation of polyamine homeostasis. Our findings provide an evidence for a potential function of OAZ1 in follicular development, ovulation and regression. 相似文献