共查询到18条相似文献，搜索用时 501 毫秒
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鸡油菌菌丝体多糖和胞外多糖分离提取的工艺要求 总被引：1，自引：0，他引：1
刘成荣 《赤峰学院学报(自然科学版)》2008,(7)
采用正交实验对鸡油菌菌丝体多糖和胞外多糖提取工艺进行了研究,结果表明鸡油菌菌丝体多糖提取的最适条件是:提取温度为80℃,乙醇终浓度为85%,提取时间为40min.在此条件下胞内多糖提取浓度最高,可达40.8μg/mL;鸡油菌胞外多糖提取的最适条件是:在6℃下用乙醇终浓度75%醇析4h胞外多糖的提取浓度最高,可达45.63μg/mL;通过正交实验结果表明影响鸡油菌菌丝体多糖提取因素为:提取温度>提取时间>乙醇浓度;影响鸡油菌胞外多糖的因素是:提取温度>乙醇浓度>提取时间. 相似文献
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试验对树舌菌丝体多糖的提取工艺进行了研究,比较了料水比、提取温度、提取次数、提取时间4个因素对多糖提取率的影响.并采用正交设计优化提取条件,确定最佳工艺条件为:料液比(g/mL)1:20、提取时间1.5h,抽提次数2次,提取温度为90℃.选择此工艺提取多糖,多糖提取率为4.06%. 相似文献
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杏鲍菇子实体多糖提取工艺优化 总被引：2，自引：0，他引：2
以杏鲍菇子实体为材料,研究了杏鲍菇子实体多糖的提取工艺.通过常规法提取多糖的单因子试验和正交试验,确定了常规法提取杏鲍菇子实体多糖的最佳工艺条件为:按料水比1∶20投料,在90℃下,提取4h,乙醇沉淀浓度为90%。 相似文献
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研究秀珍菇胞外多糖活性炭脱色工艺。以脱色率和多糖保留率为评价指标,考察活性炭用量、脱色时间、脱色温度、pH等因素对脱色效果的影响。并在此基础上,通过正交试验,确定秀珍菇胞外多糖活性炭脱色的最佳工艺:活性炭用量1.6%、脱色时间70 min、脱色温度70℃,pH3。在此条件下,色素的脱除率为80.5%,多糖的保留率为84.8%,该工艺适合秀珍菇胞外多糖脱色。 相似文献
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水提醇沉法制备商南泉茗茶茶多糖的研究 总被引：4，自引：0，他引：4
为了优化热水浸提乙醇沉淀法提取商南泉茗茶中水溶性多糖的最佳工艺。根据提取时间、提取温度、液料比以及乙醇浓度对茶多糖得率的影响情况,通过正交设计法优化商南泉茗茶水溶性多糖提取工艺。研究结果表明,影响商南泉茗茶茶多糖提取率的因素主次顺序依次为乙醇浓度>提取温度>液料比>提取时间;最佳浸提条件为提取时间2.0 h,提取温度80℃,液料比为30:1,乙醇浓度为100%;在此条件下进行实验,得到的多糖提取率可达4.15%。 相似文献
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分级灵芝多糖的分离和活性的探讨 总被引：1，自引：0，他引：1
以灵芝子实体作为实验材料,对灵芝多糖的分级提取、红外结构分析、生物活性的影响进行了研究.通过对灵芝子实体90℃热水浸提,不同乙醇浓度下醇沉量研究表明40%乙醇浓度沉淀多糖、65%乙醇浓度沉淀多糖、80%乙醇浓度沉淀多糖在灵芝粗多糖所占比例为0.31:0.27:0.42;IR分析表明65%、80%分级多糖是以β型糖苷健相连结的杂多糖;用不同分级的粗多糖以10 mg/只/天,分别对小鼠连续灌胃10天,发现分级多糖均能提高小鼠的白细胞吞噬作用和肝功能作用,但65%和80%两级较为明显. 相似文献
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文中以玉米须为原料,采用热水浸提乙醇沉淀法提取多糖.通过单因素试验,确定了提取温度、提取时间、料液比、乙醇浓度4个因素的水平.通过L9（34）正交试验,确定了最佳提取条件：提取温度为85℃,提取时间为1.5h,料液比为1：18,乙醇浓度为80%.采用苯酚硫酸法测得多糖提取率为4.83%. 相似文献
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庆大霉素发酵液的分离提取 总被引：1，自引：0，他引：1
建立从发酵液中提取庆大霉素的方法,获得具有一定纯度的庆大霉素分析样品。采用732阳离子交换树脂,利用离子交换法从发酵液中提取庆大霉素,探讨不同条件对庆大霉素收率的影响。确定最优的提取条件为:发酵液pH2～3,吸附时间2 h,解吸剂浓度5%,解吸剂流速0.4 mL/min。经HPLC测定,发酵液通过离子交换法分离提取后,庆大霉素纯度由50.7%提高到92.5%。离子交换法适用于庆大霉素发酵液的分离提取,提取液纯度达到药物分析的要求。 相似文献
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HE Guo-qing ZHANG Xiu-yan TANG Xing-jun CHEN Qi-he RUAN Hui 《Journal of Zhejiang University. Science. B》2005,6(8):825-831
The partition behaviors of β-1,3-1,4-glucanase, α-amylase and neutral proteases from clarified and whole fermentation broths of Bacillus subtilis ZJF-1A5 were investigated. An aqueous two-phase system (polyethylene glycol (PEG)/MgSO4)was examined with regard to the effects of PEG molecular weight (MW) and concentration, MgSO4 concentration, pH and NaCl concentration on enzyme partition and extraction. The MW and concentration of PEG were found to have significant effects on enzyme partition and extraction with low MW PEG showing the greatest benefit in the partition and extraction of β-glucanase with the PEG/MgSO4 system. MgSO4 concentration influenced the partition and extraction of β-glucanase significantly. pH had little effect on β-glucanase or proteases partition but affected α-amylase partition when pH was over 7.0. The addition of NaCl had little effect on the partition behavior of β-glucanase but had very significant effects on the partitioning of α-amylase and on the neutral proteases. The partition behaviors of β-glucanase, α-amylase and proteases in whole broth were also investigated and results were similar to those obtained with clarified fermentation broth. A two-step process for purifying β-glucanase was developed, which achieved β-glucanase recovery of 65.3% and specific activity of 14027 U/mg, 6.6 times improvement over the whole broth. 相似文献
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He Guo-qing Zhang Xiu-yan Tang Xing-jun Chen Qi-he Ruan Hui 《Journal of Zhejiang University. Science. B》2005,6(8):825-831
The partition behaviors of β-1,3-1,4-glucanase, α-amylase and neutral proteases from clarified and whole fermentation broths
ofBacillus subtilis ZJF-1A5 were investigated. An aqueous two-phase system (polyethylene glycol (PEG)/MgSO4) was examined with regard to the effects of PEG molecular weight (MW) and concentration, MgSO4 concentration, pH and NaCl concentration on enzyme partition and extraction. The MW and concentration of PEG were found to
have significant effects on enzyme partition and extraction with low MW PEG showing the greatest benefit in the partition
and extraction of β-glucanase with the PEG/MgSO4 system. MgSO4 concentration influenced the partition and extraction of β-glucanase significantly. pH had little effect on β-glucanase or
proteases partition but affected α-amylase partition when pH was over 7.0. The addition of NaCl had little effect on the partition
behavior of β-glucanase but had very significant effects on the partitioning of α-amylase and on the neutral proteases. The
partition behaviors of β-glucanase, α-amylase and proteases in whole broth were also investigated and results were similar
to those obtained with clarified fermentation broth. A two-step process for purifying β-glucanase was developed, which achieved
β-glucanase recovery of 65.3% and specific activity of 14027 U/mg, 6.6 times improvement over the whole broth.
Project (No. 20276064) supported by the National Nature Science Foundation of China 相似文献
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通过摇瓶培养法对真姬菇液体发酵产胞外多糖进行研究,得到产胞外多糖适宜的条件为：真姬菇液体发酵产胞外多糖的最适时间是8单位,最适pH为6．5,的最适温度为26℃、摇瓶转速为130r／min、最适装液量为50％、最适接种量为15％． 相似文献
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采用大孔树脂法分离海带多糖,并对海带多糖的体外抗氧化进行初步评价。以HZ801、HZ816、HP20、HP100、D3520五种大孔树脂为试样,探究最适分离海带多糖的树脂型号。结果表明,大孔树脂D3520对海带多糖的分离效果最好,其最适吸附条件为流速2BV·h-1、pH 5,吸附率为71.14%;洗脱的最适条件为洗脱剂乙醇体积分数45%、流速2BV·h-1,洗脱率为86.27%。药效学以抗氧化消除·OH自由基的消除率为指标,VC为阳性对照,结果表明海带多糖对·OH的有较明显消除效果,当浓度为2.0mg/mL时,清除率为38.36%,说明海带多糖具有一定抗氧化活性。 相似文献
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酶-超声提取地黄多糖及其抗氧化性研究 总被引：3，自引：0，他引：3
选用市售地黄为原料,利用纤维索酶解法结合热水浴提取法研究了提取温度、提取时间、提取液pH以及液料比对多糖提取量的影响．通过单因素试验得出地黄多糖提取工艺在液料比1：30的条件下的最佳参数为：提取温度40℃,提取时间25min,提取液pH为6．0,纤维素酶添加量为3．00％．在最佳参数组合下,多糖提取率为21．4％,地黄多糖对·OH的还原率为43．5％,此方法是简单、快捷和高效的提取方法． 相似文献
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以葛根（radix puerariae）生产葛粉后的葛渣为材料,采用乙醇回流法通过正交实验对异黄酮提取条件进行优化,同时对葛根异黄酮对金黄色葡萄球菌、大肠杆菌、枯草芽孢杆菌的抑菌作用进行研究.结果表明：以乙醇作为提取溶剂,最佳提取条件为乙醇浓度95％,提取温度70℃,料液比1∶15,提取时间120 min,葛根异黄酮的得率为1.59％;葛根异黄酮对大肠杆菌、金黄色葡萄球菌、枯草芽孢杆菌的生长都具有抑制作用,最低抑菌浓度分别为125 μg/mL、62.5 μg/mL、250μg/mL,最低杀菌浓度分别为250 μg/mL、125 μg/mL、500 μg/mL. 相似文献