共查询到18条相似文献，搜索用时 150 毫秒
1.
为了提高麦秆转化为乙醇的效率,通过正交试验对麦秆在乙醇/水中的盐酸预处理条件和乙醇/水中盐酸预处理的麦秆同步糖化发酵条件进行了优化.结果表明:乙醇/水中麦秆盐酸预处理影响的主次顺序为盐酸质量分数、时间、温度、乙醇体积分数和底物质量浓度.乙醇/水中麦秆的最佳盐酸预处理条件为温度120℃、盐酸质量分数8%、时间60 min、底物质量浓度2.5 g/L和乙醇体积分数40%,此时总还原糖浓度为469.18 mg/g.乙醇/水中盐酸对麦秆的预处理效果比水中的要好.乙醇/水中盐酸预处理的麦秆同步糖化发酵影响的主次顺序为酵母接种量、酶浓度、时间和温度.乙醇/水中盐酸预处理的麦秆最佳同步糖化发酵条件为温度33℃、酵母接种量0.3%、酶质量浓度0.8 g/L和发酵时间4 d,此时乙醇的质量浓度为22.38 g/L.乙醇/水中盐酸预处理的麦秆同步糖化发酵制乙醇的效果比水中的要好. 相似文献
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强聚硒酵母摇瓶发酵条件的优化研究 总被引：1，自引：1，他引：0
罗世炜 《襄樊职业技术学院学报》2009,8(2)
通过摇瓶培养条件试验确定了强聚硒酵母最适发酵条件:麦芽汁%,初始硒浓度20μg/mL,在其发酵进入到对数生长期后再添加亚硒酸钠使硒浓度达到60μg/mL,接种量为10%,在28℃、200 r/min的摇床上培养29 h.在此条件下,强聚硒酵母生物量(干重)达到8.25 g/L,酵母细胞中硒含量达到1230.21μg/g. 相似文献
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在前期研究的基础上,进行了强聚硒酵母发酵罐扩大培养条件的优化,最终确定发酵罐中最适发酵条件：麦芽汁1%,初始硒浓度为15μg/mL,在其发酵进入到对数生长期后再添加亚硒酸钠使之硒浓度达到25μg/mL,种子培养时间12 h,接种量为10%,培养温度28℃,通气量6 L/h,搅拌速度150 r/min,培养时间29 h。在此最优化条件下,酵母生物量达到8.05 g/L,硒含量达到1 003.19μg/g。 相似文献
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目的:研究不同浓度的栽培黑豆和野生黑豆粉末对深层发酵灵芝菌丝生长、灵芝酸产量的影响。方法:通过菌丝活化、种子液培养、液体深层发酵培养3阶段获得灵芝菌丝体,用乙醇提取灵芝酸,香草醛法测定其含量并计算产量。结果:当培养基栽培黑豆浓度为10g/L时,灵芝酸含量和产量分别比对照提高了1.59和2.66倍;当培养基野生黑豆浓度为10g/L时,灵芝菌丝体生物量和灵芝酸产量分别比对照提高2.25和3.89倍;当培养基野生黑豆浓度为4g/L时,灵芝酸含量达到最大值39.845mg/g。当培养基中野生黑豆浓度为6、8、10g/L时试验组灵芝酸产量无显著差异。结论:从经济性角度考虑,添加4g/L野生黑豆优化培养基效果最佳。 相似文献
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《绵阳师范学院学报》2021,(11):66-71
为了优化24孔板培养毕赤酵母GYW-1合成谷胱甘肽(Glutathione,GSH)的发酵条件,本文采用单因素实验和正交试验对24孔板的发酵条件进行优化.结果表明,毕赤酵母GYW-1 24孔板发酵产GSH的最优发酵条件为:酵母粉44 g/L、柠檬酸铵15 g/L、接种量12%.通过对发酵条件的优化,可以明显提高毕赤酵母GYW-124孔板发酵产GSH的产量(43.815 mg/L),较初始条件的产量增加了55%. 相似文献
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对3种共25株酵母菌进行了富铁能力的筛选,经过抗性筛选和摇瓶发酵培养筛选到一株生物量及细胞铁含量均较高的酵母菌株R4-5,该菌株能以甘蔗糖蜜作为唯一碳源发酵培养富铁酵母,在培养条件下,生物量为8.78 g/L发酵液,细胞铁含量为5.45 mg/g干菌体,总铁含量达47.851 mg/L发酵液。 相似文献
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对14份土样和7株实验室保藏菌株经初筛、摇瓶复筛,得到1株油脂产量较高的酵母菌,其油脂含量为7.34%。对影响该菌体产油脂的条件进行了单因素和正交实验,得出摇瓶发酵培养的最佳产油脂条件:发酵周期为4d,酵母膏和硫酸铵混合物为氮源,接种量为12%。在该条件下测得的菌体生物量为12.211g/L,油脂产量为1.524g/L,油脂含量为12.48%。 相似文献
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《河南职业技术师范学院学报(职业教育版)》2017,(6)
以血红铆钉菇为原料,没食子酸为对照品,总多酚得率为考核指标,研究了血红铆钉菇总多酚的水浴提取工艺条件.在提取温度与时间、乙醇体积分数及液料比等单因素的基础上,通过L16(45)正交试验,确定血红铆钉菇总多酚的最佳工艺条件为:乙醇体积分数75%,液料比30∶1(m L/g),75℃提取120 min.在上述条件下,制备的血红铆钉菇总多酚得率为17.4 mg/g(以没食子酸计). 相似文献
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对树舌发酵液中胞外多糖的提取工艺进行研究,考察发酵液浓缩比例、乙醇终浓度、pH对胞外多糖提取量的影响.在单因素试验的基础上,进行了正交优化试验,确定了树舌胞外多糖的最佳提取工艺条件为：发酵液浓缩比例为1/2、乙醇浓度为80％、pH 7.0,此条件下树舌胞外多糖提取量为0.982g/L. 相似文献
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以饲料为原料筛选出一株既产乳酸又产芽孢的益生菌,经过分子生物学鉴定为凝结芽孢杆菌(Bacillus coagulans),命名为T-8。从培养基碳源、氮源、有机物浸出液、培养基pH、培养温度、培养时间等方面对菌株T-8展开发酵特性研究,结果表明,最优培养基配方为:玉米粉6 g/L、豆粕粉15 g/L、小麦麸皮浸出液60%、酵母粉5 g/L、蛋白胨10 g/L、氯化钠10 g/L,pH 5.0。以5%的接种量将种子液转接至最优培养基,在47℃的摇床中以200 r/min的转速振荡培养28 h,菌株T-8的细胞密度达到4.8×109 CFU/mL,芽孢率达到95.2%。本试验可为益生菌凝结芽孢杆菌应用于饲用微生物菌剂的工业化生产提供参考。 相似文献
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The production of butyric acid by Clostridium butyricum ZJUCB at various pH values was investigated. In order to study the effect of pH on cell growth, butyric acid biosynthesis and reducing sugar consumption, different cultivation pH values ranging from 6.0 to 7.5 were evaluated in 5-L bioreactor. In controlled pH batch fermentation, the optimum pH for cell growth and butyric acid production was 6.5 with a cell yield of 3.65 g/L and butyric acid yield of 12.25 g/L. Based on these results, this study then compared batch and fed-batch fermentation of butyric acid production at pH 6.5. Maximum value （16.74 g/L） of butyric acid concentration was obtained in fed-batch fermentation compared to 12.25 g/L in batch fermentation. It was concluded that cultivation under fed-batch fermentation mode could enhance butyric acid production significantly （P〈0.01） by C. butyricum ZJUCB. 相似文献
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INTRODUCTION Butyric acid has several potential applications in industry. Its applications in the foodstuffs and beverage industries are widespread. It may be used as the pure acid in the dairy industry, or in the form of esters as a food additive to increase fruit fragrance. Furthermore, butyric acid could also have some important physiological functions. For example, butyric acid esters are the character-impact flavors in tropic fruits and dairy products (Centeno et al., 2002; Watson e… 相似文献
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以葛根（radix puerariae）生产葛粉后的葛渣为材料,采用乙醇回流法通过正交实验对异黄酮提取条件进行优化,同时对葛根异黄酮对金黄色葡萄球菌、大肠杆菌、枯草芽孢杆菌的抑菌作用进行研究.结果表明：以乙醇作为提取溶剂,最佳提取条件为乙醇浓度95％,提取温度70℃,料液比1∶15,提取时间120 min,葛根异黄酮的得率为1.59％;葛根异黄酮对大肠杆菌、金黄色葡萄球菌、枯草芽孢杆菌的生长都具有抑制作用,最低抑菌浓度分别为125 μg/mL、62.5 μg/mL、250μg/mL,最低杀菌浓度分别为250 μg/mL、125 μg/mL、500 μg/mL. 相似文献
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Xiao-yan ZHANG Yong PENG Zhong-rui SU Qi-he CHEN Hui RUAN Guo-qing HE 《Journal of Zhejiang University. Science. B》2013,14(2):132-143
Biotransformation of phytosterol(PS) by a newly isolated mutant Mycobacterium neoaurum ZJUVN-08 to produce androstenedione has been investigated in this paper.The parameters of the biotransformation process were optimized using fractional factorial design and response surface methodology.Androstenedione was the sole product in the fermentation broth catalyzed by the mutant M.neoaurum ZJUVN-08 strain.Results showed that molar ratio of hydroxypropyl-β-cyclodextrin(HP-β-CD) to PS and substrate concentrations were the two most significant factors affecting androstenedione production.By analyzing the statistical model of three-dimensional surface plot,the optimal process conditions were observed at 0.1 g/L inducer,pH 7.0,molar ratio of HP-β-CD to PS 1.92:1,8.98 g/L PS,and at 120 h of incubation time.Under these conditions,the maximum androstenedione yield was 5.96 g/L and nearly the same with the non-optimized(5.99 g/L),while the maximum PS conversion rate was 94.69% which increased by 10.66% compared with the non-optimized(84.03%).The predicted optimum conditions from the mathematical model were in agreement with the verification experimental results.It is considered that response surface methodology was a powerful and efficient method to optimize the parameters of PS biotransformation process. 相似文献
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Response surface methodology （RSM） was used to optimize the fermentation medium for enhancing pyruvic acid production by Torulopsis glabrata TP19. In the first step of optimization, with Plackett-Burman design, ammonium sulfate, glucose and nicotinic acid were found to be the important factors affecting pyruvic acid production significantly. In the second step, a 23 full factorial central composite design and RSM were applied to determine the optimal concentration of each significant variable. A second-order polynomial was determined by the multiple regression analysis of the experimental data. The optimum values for the critical components were obtained as follows： ammonium sulfate 0.7498 （10.75 g/L）, glucose 0.9383 （109.38 g/L） and nicotinic acid 0.3633 （7.86 mg/L） with a predicted value of maximum pyruvic acid production of 42.2 g/L. Under the optimal conditions, the practical pyruvic acid production was 42.4 g/L. The determination coefficient （R^2） was 0.9483, which ensures adequate credibility of the model. By scaling up fermentation from flask to jar fermentor, we obtained promising results. 相似文献