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1.
The AtTOM1 gene ofArabidopsis thaliana had been shown to be essential for the efficient multiplication of Tobacco mosaic virus （TMV） in A. thaliana. In this study, we cloned an AtTOM1-like gene from Nicotiana benthamiana named as NbTOM1. Sequence alignment showed that NbTOM1 is closely related to AtTOM1 homologues of N. tabacum and Lycopersicon esculentum with 97.2% and 92.6% nucleotide sequence identities, respectively. Silencing of NbTOM1 by a modified viral satellite DNA-based vector resulted in complete inhibition of the multiplication of TMV in N. benthamiana. The result suggests that inhibition of NbTOM1 via RNA silencing is a potentially useful method for generating TMV-resistant plants. 相似文献
2.
An increasing number ofmonopartite begomoviruses are being identified that a satellite molecule (DNAβ) is required to induce typical symptoms in host plants. DNAβ encodes a single gene (termed βCl) encoded in the complementary-sense. We have produced transgenic Nieotiana benthamiana and N. tabaeum plants expressing the βC1 gene of a DNAβ associated with Tomato yellow leaf curl China virus (TYLCCNV), under the control of the Cauliflower mosaic virus 35S promoter. Transgenic plants expressing 13C1 showed severe developmental abnormalities in both species. Microscopic analysis of sections of both transgenic and non-transgenic N. tabaeum leaves showed abnormal outgrowths of transgenic N. tabaeum to be due to disorganizedcell division (hyperplasia) of spongy and palisade parenchyma. Immuno-gold labeling of sections with a polyclonal antibody against the βC1 protein showed that the 13C 1 protein accumulated in the nuclei of cells. The possible biological function of the βC1 1protein was discussed. 相似文献
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为了寻求一种在烟叶生产上区别于传统防控TMV的药剂,试验以红花大金元和YK01为材料,通过田间试验探索植物有机诱导剂＂多肽保＂对TMV的防效.结果表明,＂多肽保＂兼具培育壮苗、改善烟株营养和诱导烟株产生抗病能力的功能,在育苗期对TMV的防效可达到91.8%以上.材料不同防效也不一样,在大田期栽后35 d内,易感材料YK01对TMV的防效为21.8%以上,而对红花大金元的防效则可达到80.1%以上.＂多肽保＂使用成本较低、无毒无害、操作简便,在烟叶生产上将具有较好的推广应用前景. 相似文献
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文章利用一系列的生物信息学软件分析了盐芥ATPase E Like基因编码蛋白的氨基酸组成、等电点、结构域、特征位点、二级结构等蛋白质性质,并同拟南芥ATPase E1、E2、E3蛋白作了对比.结果表明:盐芥ATPase E Like基因编码蛋白分子式为:C1132H1867N325O349S9,属于不稳定蛋白;二级结构主要是以α螺旋为主.在所分析的指标中,盐芥ATPase E Like蛋白与拟南芥ATPase E1、E2、E3相比存在着一定差异. 相似文献
5.
将含有TaLEA1基因的表达载体pBI121-TaLEA1经根癌农杆菌介导,利用花粉管通道法转入拟南芥,得T0代种子,抗Kan筛选得转基因植株.经PCR检测,初步证明外源TaLEA1基因已整合到拟南芥基因组中. 相似文献
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7.
2010-2011年期间,采用TAS？ELISA检测与电镜负染色法复检相结合,对昆明烟区14 577份苗期样品进行TMV检测与数据分析.结果表明,移栽前2-7 d,烟苗阳性检出率平均为4.13%,且烟苗的带毒率与地点、品种间关系不大,而与年度间、检测时期等关系密切.在漂浮育苗条件下,选择抗病品种是控制TMV最经济有效的措施.而对感病品种,可以通过减少剪叶次数、控制苗龄、阻断初侵染源、结合诱导抗病剂等措施,再从思想上重视、从政策上扶持,就可以实现降低烟苗带毒率、提高烟苗素质的目的. 相似文献
8.
通过不同质量分数的磷酸三钠溶液震荡浸泡烟草种子30 m in,对种子的出苗和大田烟株TMV的发病情况进行调查统计.结果表明：1）30%和40%的磷酸三钠溶液对增强种子的活力有明显效果,播种后14 d出苗率分别达到63.5%和69.2%,分别比清水对照提高了13.9%和19.6%;2）30%和40%的磷酸三钠溶液处理种子30 m in,可使大田烟株对TMV有显著的抑制效果,与其它处理的差异显著水平达到0.01;3）大田期烟株TMV的病情变化趋势,几乎不受磷酸三钠溶液处理的影响,而主要与降雨等气候因素关系密切. 相似文献
9.
基于特殊加法链的快速安全椭圆曲线标量乘算法 总被引：1，自引：0，他引：1
为了抵抗椭圆曲线密码的边信道攻击,提出了一种新型快速安全的标量乘算法.该算法是一种基于仅有点加运算的特殊加法链,可自然地抵抗边信道攻击.此外,提出在一种新型点加运算公式中引进特殊结构的加法链,可以大大提高标量乘算法的运算效率.对于长度为160比特的整数,其特殊加法链长度为260时,仅仅需要1 719次乘法运算.特殊加法链长度为280～260时,运行标量乘算法比倍点-点加算法效率上提高26%～31%,比NAF算法快16%～22%,比4-NAF算法快7%～13%,比目前最好的方法双基链算法还要快1%～8%. 相似文献
10.
选择不同抗性的8个烤烟品种（系）,在其烟苗5～7叶期接种烟草普通花叶病毒普通株系（TMV-U）,移栽人大田30 d后比较了8项生理生化指标的变化.结果表明：1）各品种质体色素含量、淀粉含量的变化与品种抗性之间无明显相关性,而MDA含量、Pro含量、NR活性和SOD活性与品种抗性之间密切相关.2）抗病品种感染TMV后SOD活性下降或明显下降,而多数感病品种体内SOD活性却呈现明显增加趋势.3）达到“免疫或抗”水平的有NC102,NC297两个品种,带毒后可能是通过体内维持MDA含量相对稳定或者大幅增加NR活性来抵抗TMV的危害;而达到中抗水平的红花大金元品种则可能是通过大幅增加自身Pro含量来抵抗TMV的危害. 相似文献
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在铁皮石斛组织培养过程中,通过筛选适宜铁皮石斛原球茎增殖、分化、壮苗和生根的培养基,以提高增殖系数和试管苗的质量。结果表明:原球茎增殖以MS NAA1．0 mg／L 6-BA1．0 mg／L KT1．0 mg／L为最佳;原球茎分化以MS NAA1．0 mg／L 6-BA3．0mg／L KT1．0 mg／L为最佳;壮苗生根以1／2MS IBA2．0 mg／L 水解酪蛋白500mg／L 10％香蕉提取液为最佳,45天生根率达92％。 相似文献
12.
Rao AQ Irfan M Saleem Z Nasir IA Riazuddin S Husnain T 《Journal of Zhejiang University. Science. B》2011,12(4):326-334
The phytochrome B (PHYB) gene of Arabidopsis thaliana was introduced into cotton through Agrobacterium tumefaciens. Integration and expression of PHYB gene in cotton plants were confirmed by molecular evidence. Messenger RNA (mRNA) expression in one of the transgenic lines,
QCC11, was much higher than those of control and other transgenic lines. Transgenic cotton plants showed more than a two-fold
increase in photosynthetic rate and more than a four-fold increase in transpiration rate and stomatal conductance. The increase
in photosynthetic rate led to a 46% increase in relative growth rate and an 18% increase in net assimilation rate. Data recorded
up to two generations, both in the greenhouse and in the field, revealed that overexpression of Arabidopsis thaliana PHYB gene in transgenic cotton plants resulted in an increase in the production of cotton by improving the cotton plant growth,
with 35% more yield. Moreover, the presence of the Arabidopsis thaliana PHYB gene caused pleiotropic effects like semi-dwarfism, decrease in apical dominance, and increase in boll size. 相似文献
13.
Molecular characterization and infectivity of Papaya leaf curl China virus infecting tomato in China 总被引：2，自引：0，他引：2
Hui Zhang Xin-ying Ma Ya-juan Qian Xue-ping Zhou 《Journal of Zhejiang University. Science. B》2010,11(2):109-114
Papaya leaf curl China virus (PaLCuCNV) was previously reported as a distinct begomovirus infecting papaya in southern China. Based on molecular diagnostic survey, 13 PaLCuCNV isolates were obtained from tomato plants showing leaf curl symptoms in Henan and Guangxi Provinces of China. Complete nucleotide sequences of 5 representative isolates (AJ558116, AJ558117, AJ704604, FN256260, and FN297834) were determined to be 2738–2751 nucleotides, which share 91.7%–97.9% sequence identities with PaLCuCNV isolate G2 (AJ558123). DNA-β was not found to be associated with PaLCuCNV isolates. To investigate the infectivity of PaLCuCNV, an in-fectious clone of PaLCuCNV-[CN:HeNZM1] was constructed and agro-inoculated into Nicotiana benthamiana, N. tabacum Samsun, N. glutinosa, Solanum lycopersicum and Petunia hybrida plants, which induced severe leaf curling and crinkling symptoms in these plants. Southern blot analysis and polymerase chain reaction (PCR) indicated a systemic infection of test plants by the agro-infectious clone. 相似文献
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Zhao JH Zhang JS Wang Y Wang RG Wu C Fan LJ Ren XL 《Journal of Zhejiang University. Science. B》2011,12(11):935-942
DNA methylation plays an important role in the epigenetic regulation of gene expression during plant growth, development,
and polyploidization. However, there is still no distinct evidence in tobacco regarding the distribution of the methylation
pattern and whether it contributes to qualitative characteristics. We studied the levels and patterns of methylation polymorphism
at CCGG sites in 48 accessions of allotetraploid flue-cured tobacco, Nicotiana tabacum, using a methylation-sensitive amplified polymorphism (MSAP) technique. The results showed that methylation existed at a
high level among tobacco accessions, among which 49.3% sites were methylated and 69.9% allelic sites were polymorphic. A cluster
analysis revealed distinct patterns of geography-specific groups. In addition, three polymorphic sites significantly related
to tobacco mosaic virus (TMV) resistance were explored. This suggests that tobacco breeders should pay more attention to epigenetic
traits. 相似文献
16.
文章通过筛选适宜金线莲丛芽诱导、增殖和生根的培养基和研究影响试管苗移栽的条件，建立起金线莲组织培养的技术体系。结果表明：金线莲丛芽诱导以MS+6-BA30 mg/L +NAA05 mg/L +KT10 mg/L为最佳；丛芽增殖以MS+6-BA30 mg/L +NAA05 mg/L为最佳；生根以1/2MS+IBA10 mg/L +NAA10 mg/L水解酪蛋白05%+05%活性碳为最佳；在移栽中，以混合土（普通土：沙质土：有机肥=1∶1∶1）作为基质为较好，移栽成活率达到909% 相似文献
17.
杨平 《南京晓庄学院学报》2011,27(3):72-75
该研究分析了拟南芥9个β-淀粉酶家族基因（BAM1―BAM9）在cDNA和蛋白质上的同源性,分析了每个基因反映密码子使用特性的参数：GC含量、同义密码子第1～3位置中的GC含量、有效密码子数等.结果表明,拟南芥β-淀粉酶家族基因在密码子的使用特性上不存在的偏好性.Spss分析表明,ENc与GC3s%和GC%呈显著正相关（P〈0.05）.拟南芥β-淀粉酶家族基因序列间的同源性差异较大,在cDNA和蛋白质序列上的基因同源性分别为41.6%～64.2%和29.4%～62.6%,其中,BAM5和BAM6间的同源性最高. 相似文献
18.
目前,人们研究肿瘤的热点已经从癌基因转到抑癌基因,在已发现的数个抑癌基因中,p53、Fas、p16、Fhit、nm23、BLCAP与宫颈癌的关系密切,也是宫颈癌研究的热点.p53和p16基因通过调控细胞周期来抑制细胞增殖;Fas通过与其配体FasL或其单克隆抗体结合后,诱导Fas所在细胞凋亡;Fhit通过诱导细胞凋亡、细胞周期阻滞发挥着抑制肿瘤细胞增殖的作用;nm23则是通过改变nm23基因的编码产物二磷酸核苷酸激酶(NDPK)从而引起NTP产生不足,参与浸润、转移过程;BLCAP是一个新近发现的宫颈癌相关抑癌基因,其功能及性质尚不大清楚. 相似文献
19.
对不同烤烟品种施用＂AHO＋多肽保＂组合防控剂,比较＂AHO＋多肽保＂组合防控剂诱导不同烤烟品种抗TMV效果的差异.结果显示：＂AHO＋多肽保＂组合剂诱导不同品种抗TMV的效果差异有统计学意义;对红大的诱导效果最好,云87次之,再次是K326,对云97和KRK26的诱导效果最差.相关性分析结果显示：不同品种移栽前发病率和苗期带毒率呈显著正相关;不同品种大田移栽后病情指数与苗期带毒率也呈显著正相关. 相似文献