共查询到20条相似文献，搜索用时 101 毫秒
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目的:探讨新城疫病毒(Newcastle disease virus,NDV)体外对S180肉瘤细胞(简称S180)生长有无抑制和诱导细胞凋亡作用。方法:取培养至对数生长期的S180细胞浓度为5×104/mL和1×106/mL分别接种于96孔板和50mL培养瓶中,加入不同浓度的NDV(1∶20、1∶10、1∶5)作用24h,分别用MTT法检测NDV对S180瘤细胞的生长抑制作用,荧光显微镜和琼脂糖凝胶电泳检测细胞凋亡。结果:经上述不同浓度的NDV作用24h,对S180细胞生长的抑制率依次为10%、26%、39%,各实验组吸光度值(A490)与对照组比较差异均有统计学意义(P<0.05)。荧光显微镜观察实验组培养的S180瘤细胞,出现细胞核固缩及凋亡小体,琼脂糖凝胶电泳见DNA梯形条带。结论:NDV对体外培养S180细胞增殖有明显抑制作用,可诱导S180细胞凋亡。 相似文献
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目的:观察姜黄素对人乳腺癌MCF-7细胞(简称MCF-7细胞)凋亡的诱导作用.方法:不同浓度姜黄素(0.048,0.056,0.064,0.072,0.080μmol/L)作用于MCF-7细胞24h,采用CCK-8法检测其对MCF-7细胞的生长抑制作用;琼脂糖凝胶电泳检测细胞凋亡结果.结果:经上述不同浓度姜黄素诱导24h后,MCF-7细胞生长抑制率分别为17%、28%、48%、50%及55%;琼脂糖凝胶电泳显示出DNA梯形条带.结论:姜黄素对体外培养MCF-7细胞的增殖有明显的抑制作用并可诱导其细胞凋亡. 相似文献
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目的:探讨刺五加注射液对人宫颈癌细胞株Hela体外增殖的抑制作用。方法:采用MTT法测定了不同浓度的刺五加注射液对人宫颈癌细胞株Hela的体外抑制作用,倒置显微镜观察细胞形态变化。结果:刺五加注射液浓度越高对Hela细胞抑制作用越好,作用时间越长抑制效果越强,量效关系和时效关系良好;形态学观察到细胞凋亡。结论:刺五加注射液对人宫颈癌细胞株Hela的细胞增殖有较强的抑制作用。 相似文献
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目的:研究凋亡调控相关蛋白来了解顺铂耐药成因,同时考察乙烷硒啉(Ethaselen)在K562耐药细胞中逆转顺铂耐药的作用,并初步探讨其作用机制。创新点:首次研究乙烷硒啉在逆转顺铂耐药中的作用,且此作用与乙烷硒啉诱导细胞凋亡相关。方法:通过长时间脉冲诱导得到顺铂耐药K562细胞,并观察耐药细胞形态及倍增时间。采用MTT法考察乙烷硒啉、顺铂及其联用组在不同细胞株间的生长抑制作用。流式细胞术分析细胞凋亡情况以及细胞内活性氧(ROS)水平。最后,通过蛋白质免疫印迹(Western blot)考察凋亡调控相关蛋白水平的变化。结论:脉冲诱导得到的K562耐药细胞对顺铂的耐受性是原K562细胞的5.34倍。形态学观察发现,耐药细胞体积增大,粘附性进一步降低。乙烷硒啉与顺铂联用表现出协同效应。当加入少量的乙烷硒啉(顺铂与乙烷硒啉的摩尔比率为10:1),顺铂作用K562耐药细胞的半抑制浓度(IC50)值可以减少21倍。流式细胞术及Western blot表明,乙烷硒啉能够诱导耐药细胞凋亡。其逆转顺铂耐药主要是通过调控Bcl-2及Bax蛋白比例以及通过提高细胞内活性氧水平引起线粒体通透转运孔道(PTP)蛋白孔道的形成来促使释放细胞色素c,进而引起Caspase凋亡途径。 相似文献
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通过观察不同剂量二甲基亚砜(dimethyl sulfoxide DMSO)作用下PC12细胞形态和生长状态的变化,确定PC12细胞对DMSO的耐受剂量.按照一定浓度梯度(V/V)将DMSO加入培养液干预后的24、48和72 h于倒置相差显微镜下观察AO/EB染色前后细胞的形态特征,同时用四甲基偶氮唑蓝(MTT)显色法检测细胞的生存率和计算半数抑制浓度(50%inhibiting concentration,IC50).结果表明:DMSO浓度为14 mL/L时,24 h内90%的PC12细胞生长正常,形态完整,活力与对照组比较无差别,浓度提高到20 mL/L时,24 h时细胞生存率可下降27%,随着作用时间延长,细胞生存率进一步降低;24、48和72 h对应的IC50值分别为31.4、23.9和19.2 mL/L.因此,对PC12细胞而言,24 h内的应用终浓度应低于14 mL/L,干预时间如需延长,DMSO的应用剂量应相应降低. 相似文献
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目的:通过观察大蒜素对体外培养的人上皮性卵巢癌耐顺铂细胞株(SKOV-3/DDP)的超微结构的影响,以期寻找对耐顺铂治疗的上皮性卵巢癌患者的有效治疗药物。方法:细胞培养:体外培养人上皮性卵巢癌耐顺铂细胞株SKOV-3/DDP,细胞生长至对数生长期后,调整细胞密度为5×l04/ml置于200ml的培养瓶中培养。用透射电子显微镜观察大蒜素作用后SKOV-3/DDP细胞的超微结构:大蒜素组用含25μg/ml大蒜素的培养液培养SKOV-3/DDP细胞,空白对照组用不含药的培养液培养SKOV-3/DDP细胞,两组作用24小时后收集细胞,通过透射电子显微镜观察SKOV-3/DDP的超微结构。结果:大蒜素对SKOV-3/DDP细胞超微结构的影响:透射电子显微镜观察未加药物培养的SKOV-3/DDP细胞呈椭圆形,细胞核为卵圆形,未出现凋亡细胞,经过大蒜素作用后的SKOV-3/DDP细胞则出现明显的超微结构改变并具有典型凋亡细胞特征:细胞核固缩,核膜皱缩,胞浆浓缩并出现大小不等的空泡,染色质浓聚,形成凋亡小体。结论:大蒜素可诱导SKOV-3/DDP细胞呈凋亡改变。大蒜素可诱导SKOV-3/DDP细胞凋亡可能是其对人耐顺铂上皮性卵巢癌的抗癌机制之一,为使大蒜素成为治疗耐顺铂上皮性卵巢癌患者的有效药物提供了可靠的体外实验依据。 相似文献
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商南泉茗茶多糖的体外抗肿瘤活性 总被引：2，自引：0，他引：2
为了研究商南泉茗茶多糖体外抑瘤作用,采用MTT法检测其作用于结肠癌、肺癌、乳腺癌等肿瘤细胞系后的细胞存活率、LDH法检测细胞毒性、流式细胞术分析细胞周期和细胞凋亡情况。结果表明,商南泉茗茶多糖对结肠癌、肺腺癌、乳腺癌等肿瘤细胞系均有不同程度的生长抑制作用,其中对乳腺癌MCF-7细胞的增殖抑制作用最强,结肠癌次之,肺癌最弱。进一步研究表明,商南泉茗茶多糖能够使MCF-7细胞的生长周期阻滞于G0/G1期,并且有明显的诱导MCF-7细胞凋亡作用。商洛绿茶茶多糖具有明显的体外抗肿瘤活性。 相似文献
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邱崇丙 《唐山师范学院学报》2014,(1):21-23
汉字的六书,《说文》对“转注”一类,语焉不详。后世学者提及转注,也仅限于许慎所举出的例字。《汉字例说》一文,作者从转注的角度综合考虑,对部分现代常用字作了分析,跟传统的解释有所不同。希望能抛砖引玉,互相切磋,以推进学术研究。 相似文献
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《海外英语》2007,(4):36
There are numbers of crossroads on our long and unpredictable life journey where we totally have no idea about which direction to choose. No matter what our decision is, we should not turn back, but face the music and go ahead instead. I am this kind of girl who always does try without regretting, one example is how I dealt with my love. 相似文献
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Migration occurs behind a variety of reasons and has a great effect on the whole world. People may migrate in order to improve their economic situation, or in order to escape civil strife, persecution, and environmental disasters. The impact of migration is complex, bringing both benefits anddisadvantages. This paper briefly talks about the causes of migration, the allocation of benefits, and the ways in which individual countries and the international community deal with this important subject. 相似文献
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Sister Carrie is one of the most controversial characters in American literature.Thought as a "fallen woman" firstly,she was defined as a "new woman" by some critics later. However, by digging into the motivaton behind the whole process of Carrie's "success", the relationship between Carrie and her creator (the author), the social conditions of then American, it can be found that Carrie has never been free-standing on her thought and she has never found her real-sdf even after becoming a famous actress. In a society dominated by mass consumerism Carrie is only an adherent of her own desires. She also is a representative of all those country girls flooded into cities, a symbol and a sacrifice of the urbanization of America in a time countryside was overcome by cities. 相似文献
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RUZBEHANI Mohsen 《重庆大学学报(英文版)》2004,3(1):20-25
1.IntroductionOne-cyclecontrolmethod,whichwasproposedaboutonedecadeago[1],hasbecomeanattractivemethodinspecialfieldssuchaspowerfactorcorrection[2-6],switchingamplifiers[7,8],etc.Themainideaofthiscontrollerisbasedonintegrationofdiodevoltageinone-cycleandforcesittobeexactlyequaltothereferencevalue.Themainadvantageofthiscontrollerisitsrealtimeabilitytorejectthevariationofinputvoltage[1].Despitethisgreatability,ithasnogoodperformancesinrejectingofloaddisturbanceandfollowingreferencecommands.Espec… 相似文献
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Rachel Zupek 《海外英语》2007,(11):40-41
我们绝大部分人都同意一件事情——我们喜欢金钱。但是你对金钱的喜欢之情足够到让你创立一份事业么?.财富通常被人忽视,主要在于人们不能真正了 相似文献
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YANGShiguo 《重庆大学学报(英文版)》2004,3(1):86-88
The problem on the geometrc inequalities involving an n-dimensional simplex and its inscribed simplex is studied. Aninequality is established, which reveals that the difference between the squared circumradius of the n-dimensional simplex andthe squared distance between its circumcenter and barycenter times the squared circumradius of its inscribed simplex is not lessthan the 2(n-1)th power of n times its squared inradius, and is equal to when the simplex is regular and its inscribed siplex is atangent point one. Deduction from this inequality reaches a generalization of n-dimensional Euler inequality indicating that thecircumradius of the simplex is not less than the n-fold inradius. Another inequality is derived to present the relationship betweenthe circumradius of the n-dimensional simplex and the circumradius and inradius of its pedal simplex. 相似文献
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Rajesh Kanoi 《海外英语》2007,(1):52
Today, Sunday morning, I sit thinking and typing at my computer. My thoughts are in my homeland, India and my body is in China. The body is here and mind there for a good reason. Mr. Hu is president and I am resident and both are in each other's countries (what a difference the absence of one small letter makes!). Mr. Hu will return in a few days and I will stay on longer. 相似文献