Enhancing cellular immune response to HBV M DNA vaccine in mice by codelivery of interleukin-18 recombinant     

陈建忠  朱海红  刘克洲  陈智 《浙江大学学报(A卷英文版)》2004,5(4):467-471

Objective: To investigate the effect of interleukin-18 (IL-18) on immune response induced by plasmid encoding hepatitis B virus middle protein antigen and to explore new strategies for prophylactic and therapeutic HBV DNA vaccines. Methods: BALB/c mice were immunized with pCMV-M alone or co-immunized with pcDNA3-18 and pCMV-M and then their sera were collected for analysing anti-HBsAg antibody by ELISA; splenocytes were isolated for detecting specific CTL response and cytokine assay in vitro. Results: The anti-HBs antibody level of mice co-immunized with pcDNA3-18 and pCMV-M was slightly higher than that of mice immunized with pCMV-M alone, but there was not significantly different (P>0.05). Compared with mice injected with pCMV-M, the specific CTL cytotoxity activity of mice immunized with pcDNA3-18 and pCMV-M was significantly enhanced (P<0.05) and the level of IFN-γ in supernatant of splenocytes cultured with HBsAg in vitro was significantly elevated (P<0.05) while the level of IL-4 had no significant difference (P>0.05). Conclusion: The plasmid encoding IL-18 together with HBV M gene DNA vaccines may enhance specific TH1 cells and CTL cellular immune response induced in mice, so that IL-18 is a promising immune adjuvant.  相似文献   

Usage patterns and social circles on Facebook among elderly people with diverse personality traits     

Fan Mo  Michal Kosinski  David Stillwell 《Educational gerontology》2018,44(4):265-275

Elderly people are exposed to information technologies to keep them in touch with younger generations. Among various technologies, social network sites (SNSs) are seldom used by the majority of elderly people. To bridge the digital divide, it is necessary to dig deeply into the minority elderly users of SNSs. This study explores usage patterns of elderly Facebook users and further investigates how their diverse characteristics influence their usage and social circles. Three findings were derived. First, a sharp digital divide on SNSs does exist, and existing elderly Facebook users are mainly the dabblers. Second, personality profiling could predict elderly people’s SNS usage behaviors. Elderly users with a high level of openness and extroversion have more friends, status updates, and group memberships, and those with a high level of conscientiousness have fewer likes and status updates. Elderly users are likely to seek insightful content on SNSs. Moreover, they may have a higher degree of emotional stability than young users when using SNSs. Third, elderly people tend to build small social circles on SNSs that “copy” the family relationship in the real world; whereas, they build large social circles with interests and hobbies that are difficult to share in the real world. Based on these results, detailed suggestions are presented for better targeting elderly people in ways that move them toward SNSs.  相似文献   

Editorial     

Magdalena Mo Ching Mok 《教育心理学》2016,36(4):613-615

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Tongan students’ attitudes towards their subjects in new zealand relative to their academic achievement     

Mo’ale Otunuku  Gavin T. L. Brown 《Asia Pacific Education Review》2007,8(1):117-128

Research indicates that affective attitudes such as liking of a subject and confidence in one’s ability within a subject predict academic performance. Generally, immigrant minority students have positive attitudes and often have low academic performance. This study examines the self-efficacy and liking of subjects of New Zealand students and analyses the relationship of those attitudes towards academic performance in mathematics, writing, and reading by self-reported ethnicity. Data were obtained from the norming samples from the Assessment Tools for Teaching and Learning project in New Zealand. Of special interest are the relationships between attitude and performance for Pasifika and Tongan students in New Zealand. Tongan and Pasifika students had positive attitudes, but their mean scores were not significantly different to other ethnic groups except in writing for Tongan students. Tongan and Pasifika students did have lower academic performance than majority and Asian immigrant students in all three subjects. The correlation between liking and self-efficacy was fundamentally zero for Tongan and Pasifika students, while it was weakly positive for majority and Asian immigrant students. Together these results question the power of self-efficacy and liking attitudes to predict academic performance for immigrant students from agrarian or traditional societies. Further, the data suggest that ‘school effects’ are most likely explanations for this relationship, rather than lack of attachment, opposition, or deficiency theories.  相似文献   

甘肃方言特点举要(一)   总被引：2，自引：0，他引：2  

张成材  莫超 《甘肃高师学报》2005,10(3):30-35

甘肃中、南部汉语方言分属于兰银官话、中原官话及西南官话．本文归纳、比较了数十个县声调、声母、韵母的同异,并从语法角度进行了人称代词、“数－量－名”结构及介词“拿”的音读与用法的比较,为官话方言下位分区提供实证材料．  相似文献   

儿童多动行为案例分析     

莫烘霞 《基础教育研究》2006,(5):48-49

一、个案基本材料乐乐,男,6岁半,小学一年级学生。父母亲是公司职员。二、问题行为概述刚接一年级,一切都是新的开始。在刚开学几天紧张的工作中,我的耳边听到的最高频率的一个名字是“乐乐”。孩子们向我汇报:“老师,乐乐不进教室上课。”“老师,乐乐骂我们。”同事们向我诉苦  相似文献   

《土力学》实验课教学中的几点思考   总被引：2，自引：0，他引：2  

莫振龙 《科技广场》2007,(4):159-160

本文对土力学实验教学的现状作了分析,并对将来教学的发展提出了若干具体的措施。  相似文献   

胃镜污染HBV、HCV和HIV的调查   总被引：1，自引：0，他引：1  

周林福  朱海红  戴一扬  胡敏君  周云连  黄新  方洁  陈智 《科技通报》2004,20(1):70-72

目的　检测胃镜常规清洗消毒后HBV、HCV、HIV污染残留状况.方法　随机取样.巢式聚合酶链反应法检测HBV DNA,逆转录巢式聚合酶链反应法检测HCV RNA、HIV RNA,放免法检测HBsAg.结果　对605例标本进行检测,未检出阳性标本.结论　胃镜经常规消毒后未检到HBV、HCV和HIV的污染,但进一步规范胃镜消毒操作方法,提高消毒质量,意义重大.  相似文献   

我国科技期刊市场环境的SWOT分析   总被引：7，自引：3，他引：4  

莫京 《编辑学报》2007,19(1):53-55

利用SWOT分析法分析了我国科技期刊市场环境的优势(strengts)、劣势(weaknsses),以及存在的机遇(opponunities)和威胁(threats).认为我国科技期刊市场环境的优势是产品替代性小、可利用资源丰富;劣势为期刊的影响力亟待提高、办刊思路滞后、发行量小、市场化程度低;威胁是外资和外刊进入、信任度危机、广告竞争加剧;目前我国科技期刊的发展机遇是科技形势的发展、国家宏观经济体制的改革、消费水平不断提高和期刊对外合作的日益加强.  相似文献   

唠叨话的语用学分析     

莫国辉  章礼霞 《北京工业职业技术学院学报》2007,6(1):57-60

从语用学的角度,根据Grice的会话合作原则和Leech提出的礼貌准则,对唠叨话进行深层分析探究.唠叨话,之所以通常被认为是失败的交际话语,是因为其严重违反了合作原则中的数量准则、关联准则和方式准则;归属于竞争类和冲突类的言外行为,唠叨话常常违反了礼貌原则中的策略准则和赞扬准则.  相似文献