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Objective: To construct a PC12 cell strain with neuronal differentiation, and observe the apoptosis and pro-liferation activity effects induced these cells by Amyloid beta-Protein (Aβ-43). Methods: 1) PC12 cells in logarithmicgrowth phase were subcultured for 24 h. After the culture fluid was changed, the cells were treated with Rat-β-NGF andcultured for 9 days. 2) Neuronal differentiation of PC12 cells in logarithmic growth phase were divided into four groups:control group (0), experimental group (1), experimental group (2) and experimental group (3). The concentrations of Aβ inthe four groups were 0 μmol/L, 1.25 μ mol/L, 2.5 μ mol/L and 5 μmol/L, respectively. The cells were harvested at 24, 48 and72 h later and stained with AnnexinV-FITC/PI after centrifugation and washing. Then flow cytometry was conducted toexamine the apoptosis percentage. 3) NGF-induced PC12 cells were selected and Aβ with different concentrations wasadded. The final concentrations of Aβ were 0 μmol/L, 1.25 μmol/L, 2.5 μmol/L and 5 μ mol/L, respectively. After the cellswere incubated in an atmosphere of 5% CO2 at 37 ℃ in an incubator for 72 h, the OD values were examined. Results: 1)Neuronal differentiated PC 12 cell lines were successfully established. 2) Flow cytometric examination indicated that Aβ(1.25, 2.5, and 5.0 μmol/L) could effectively induce apoptosis of neuronal-differented cells at the 24 h, 48 h and 72 h timepoints. 3) Aβ (0-5.00 μ mol/L) had no obvious effect on proliferation or restraining of the neuronal differentiation of thePC 12 cells after a 72 h interacting process. Conclusion: This investigation revealed successful neuronal differentiation of thePC12 cell strain. The induction of apoptosis of the neurocytes by various concentrations of Aβ was observed and the in-fluence of Aβ on induced proliferation of PC 12 cells by Rat-β-NGF was revealed. This study -05 provide basis for futureresearch on the molecular cure of AD and interdiction of AD evolution. 相似文献
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Objective: To investigate the effect of interleukin-18 (IL-18) on immune response induced by plasmid encoding hepatitis B virus middle protein antigen and to explore new strategies for prophylactic and therapeutic HBV DNA vaccines. Methods: BALB/c mice were immunized with pCMV-M alone or co-immunized with pcDNA3-18 and pCMV-M and then their sera were collected for analysing anti-HBsAg antibody by ELISA; splenocytes were isolated for detecting specific CTL response and cytokine assay in vitro. Results: The anti-HBs antibody level of mice co-immunized with pcDNA3-18 and pCMV-M was slightly higher than that of mice immunized with pCMV-M alone, but there was not significantly different (P>0.05). Compared with mice injected with pCMV-M, the specific CTL cytotoxity activity of mice immunized with pcDNA3-18 and pCMV-M was significantly enhanced (P<0.05) and the level of IFN-γ in supernatant of splenocytes cultured with HBsAg in vitro was significantly elevated (P<0.05) while the level of IL-4 had no significant difference (P>0.05). Conclusion: The plasmid encoding IL-18 together with HBV M gene DNA vaccines may enhance specific TH1 cells and CTL cellular immune response induced in mice, so that IL-18 is a promising immune adjuvant. 相似文献
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小强,男,高中二年级的学生.小强第一次来找我是今年5月的一个中午.他进来的时候,神情沮丧,显得很痛苦的样子.看到他的表情,我当时就断定,面前的这个同学不是简单的学习困扰或者说是单纯的同学交往问题.果然不出所料,该生正被早恋的苦恼困扰着. 相似文献
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2018年《关于全面深化新时代教师队伍建设改革的意见》对教师继续教育提出了新要求,也为思政课教师的继续教育指明新方向和创新的新源泉.因此,文章在基于高校思政课教师继续教育现状分析的基础上,依据文件精神,紧紧把握政治方向,将文件精神融入继续教育全过程中,提出立德树人,强化继续教育德育功能;融合情景教育教学,构建"逆序创新"教育模式等对策,以解决新时代高校思政课教师继续教育所存在的问题. 相似文献
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随着全球可持续发展的持续推进,各国对ESG日益重视。在实现“双碳”目标、创建绿色学校、推进国家治理体系和治理能力现代化的背景下,高校对环境保护、社会责任以及高校治理的重视程度日益提高,然而,高校ESG方面的负面事件却频频曝光,高校ESG信息披露亟须加强。有鉴于此,在梳理和借鉴国际代表性ESG信息披露标准的基础上,从高校ESG信息披露的目标与原则、内容框架、披露方式等方面构建高校ESG信息披露框架,以期为我国制定高校ESG信息披露标准以及提升高校ESG表现提供借鉴。 相似文献
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科学技术:教育与生产劳动相结合的中介陈智,宋烈侠,阎桂芝,王晓阳教育与生产劳动相结合(以下简称教劳结合)是我国教育方针的基本内容之一。正确执行教劳结合方针的前提是正确理解教劳结合的方针,而要正确理解教劳结合的方针,我们认为,一要正确理解革命导师对教劳... 相似文献