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M Maneesh H Jayalekshmi T Suma S Chatterjee A Chakrabarti T A Singh 《Indian journal of clinical biochemistry : IJCB》2005,20(1):129-130
Evidence of increased oxidative stress in patients of osteoarthritis in comparison with healthy control subjects was investigated
by measuring the thiobarbituric acid reactive substances (TBARS), vitamin C, reduced glutathione (GSH) and the activities
of superoxide dismutase (SOD), catalase and glutathione peroxidase (GPx) in erythrocytes. It was observed that osteoarthritis
patients were more susceptible to oxidative damage than controls as evident from increased TBARS and decreased ascorbic acid,
GSH, catalase and GPx in erythrocytes. Significant increase in SOD activity found in patients might be an adaptive response.
With the understanding of the role of antioxidants in arthritis, it is becoming increasingly clear that these agents seem
to be beneficial in osteoarthritis. 相似文献
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Pranab S. Basu Ramdhan Majhi Sudip Chatterjee Sandip K Batabyal 《Indian journal of clinical biochemistry : IJCB》2000,15(2):119-123
A method has been developed to separate and identify thyroglobulin autoantibody (TgAb) and thyroid peroxidase autoantibody
(TPOAb) in serum obtained from normal and autoimmune thyroid diseases using phenyl Sepharose CL-4B hydrophobic column. The
protein peaks obtained from hydrophobic column were identified as TgAb and TPOAb by comparing the elution profile of commercially
purified standard thyroid autoantibodies. The similarity of the inhibitory effects of eluted proteins and of standard thyroid
autoantibodies on lectin concanavalin A-RBC interaction confirmed the separation of TPO-Ab and TgAb by the hydrophobic, column.
The eluted fractions from the hydrophobic column were estimated by the radio immunoassay (RIA) to confirm the presence of
both auto-antibodies. This hydrophobic column method offers an advantage of visual inspection of this autoantibodies by graphic
representation of peak height along with their estimation in autoimmune thyroid disorders. 相似文献
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Harshini Sriram Twinkle Khanka Shweta Kedia Priyanka Tyagi Sitaram Ghogale Nilesh Deshpande Gaurav Chatterjee Sweta Rajpal Nikhil V Patkar Papagudi G Subramanian Sumeet Gujral Syed Hasan Prashant R Tembhare 《Biochemia medica : ?asopis Hrvatskoga dru?tva medicinskih biokemi?ara / HDMB》2021,31(3)
IntroductionMicroRNAs are small, non-coding RNA molecules that are becoming popular biomarkers in several diseases. However, their low abundance in serum/plasma poses a challenge in exploiting their potential in clinics. Several commercial kits are available for rapid isolation of microRNA from plasma. However, reports guiding the selection of appropriate kits to study downstream assays are scarce. Hence, we compared four commercial kits to evaluate microRNA-extraction from plasma and provided a modified protocol that further improved the superior kit’s performance.Materials and methodsWe compared four kits (miRNeasy Serum/Plasma, miRNeasy Mini Kit from Qiagen; RNA-isolation, and Absolutely-RNA MicroRNA Kit from Agilent technologies) for quality and quantity of microRNA isolated, extraction efficiency, and cost-effectiveness. Bioanalyzer-based Agilent Small RNA kit was used to evaluate quality and quantity of microRNA. Extraction efficiency was evaluated by detection of four endogenous control microRNA using real-time-PCR. Further, we modified the manufacturer’s protocol for miRNeasy Serum/Plasma kit to improve yield.ResultsmiRNeasy Serum/Plasma kit outperformed the other three kits in microRNA-quality (P < 0.005) and yielded maximum microRNA-quantity. Recovery of endogenous control microRNA i.e. hsa-miR-24-3p, hsa-miR-191-5p, hsa-miR-423-5p and hsa-miR-484 was higher as well. Modification with the inclusion of a double elution step enhanced yield of microRNA extracted with miRNeasy Serum/Plasma kit significantly (P < 0.001).ConclusionWe demonstrated that miRNeasy Serum/Plasma kit outperforms other kits and can be reliably used with a limited plasma quantity. We have provided a modified microRNA-extraction protocol with improved microRNA output for downstream analyses. 相似文献
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Partha Chatterjee 《Inter-Asia Cultural Studies》2013,14(1):116-126
Abstract In the last quarter of a century there has been a fundamental change in the historical situation of post‐coloniality. The new conditions under which global flows of capital, commodities, information and people are now regulated have created both new opportunities and new obstacles for post‐colonial countries. The old idea of a Third World, sharing a common history of colonial oppression and backwardness, is no longer as persuasive as it was in the 1960s. The phenomenal growth of China and India in recent years has set in motion a process of social change that, in its scale and speed, is unprecedented in human history. I will argue that the forms of capitalist industrial growth in the twenty‐first century may, in large agrarian countries like China, India and the countries of South‐east Asia, make room for the preservation of peasant production and peasant cultures, but under completely altered conditions. The analysis of these emergent forms of postcolonial capitalism requires new conceptual work. 相似文献
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M. Lama M. Chatterjee T. K. Chaudhuri 《Indian journal of clinical biochemistry : IJCB》2013,28(2):197-200
Immunoglobulin (Ig) E has been shown to be a major contributing factor for the development of bronchial hyperresponsiveness in asthma. An elevation in serum IgE levels contributes to asthma and is considered a potent predictor of the development of asthma. The objectives of the present study were to estimate the levels of total serum IgE in asthmatic and healthy control subjects and to investigate the relationship of various demographic and clinical characteristics with the total serum IgE level in asthmatics. We measured the levels of total serum IgE using the ELISA kits (AccuBind, Monobind Inc., USA). The relevant demographic and clinical data were obtained using the questionnaire. The results showed that asthmatic children had significantly elevated level of total serum IgE compared to that of the healthy controls. The levels of total IgE and IL-4 in sera of 44 asthmatic children showed a significant positive correlation. Total serum IgE >150 IU/mL was found to be significantly associated with the age, exposure to cigarette smoke, and raised eosinophil count in asthmatic children. In conclusion, the elevated level of total serum IgE may demonstrate the allergic etiology of asthma in the subjects studied. 相似文献
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Mayukh Chatterjee 《Resonance》2006,11(9):55-71
The phenomenon of dispersal has been defined in many different ways, of which one of the oldest definitions was the one given
by Lidicker in 1975 [1]. He defined dispersal as“…any movement of individual organisms or their propagules in which they leave their home area, sometimes establishing a new
home area. This does not include short-term exploratory movements, or changes in the boundaries of a home range, such that
the new range includes at least part of the former. Dispersal thus produces homeless travelers (vagrants) who are in search
of a new home.” Dispersal has been at the forefront of research involving animal behaviour and ecology for a very long time. 相似文献
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Permeating across societies and cultures, music is a companion to millions across the globe. Despite being an abstract art form, music affects all of us deeply, encouraging us in periods of difficulty, comforting us in moments of sadness and celebrating with us in times of joy. In recent years, neuroscientists have recognized that listening to and producing music involves a tantalizing mix of practically every human cognitive function. Thus, music has emerged as an invaluable tool to study varying aspects of the human brain such as auditory and motor perception and learning, attention, memory, and emotion. In this paper, we will discuss the different attributes of music and the neural structures associated with them. We will also highlight some recent work in our laboratory at the National Brain Research Centre in India on emotions associated with Hindustani rāga music. 相似文献
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