Association of hypomethylation of LINE-1 repetitive element in blood leukocyte DNA with an increased risk of hepatocellular carcinoma     

Di JZ  Han XD  Gu WY  Wang Y  Zheng Q  Zhang P  Wu HM  Zhu ZZ 《Journal of Zhejiang University. Science. B》2011,12(10):805-811

Global DNA hypomethylation has been associated with increased risk for cancers of the colorectum, bladder, breast, head and neck, and testicular germ cells. The aim of this study was to examine whether global hypomethylation in blood leukocyte DNA is associated with the risk of hepatocellular carcinoma (HCC). A total of 315 HCC cases and 356 age-, sex- and HBsAg status-matched controls were included. Global methylation in blood leukocyte DNA was estimated by analyzing long interspersed element-1 (LINE-1) repeats using bisulfite-polymerase chain reaction (PCR) and pyrosequencing. We observed that the median methylation level in HCC cases (percentage of 5-methylcytosine (5mC)=77.7%) was significantly lower than that in controls (79.5% 5mC) (P=0.004, Wilcoxon rank-sum test). The odds ratios (ORs) of HCC for individuals in the third, second, and first (lowest) quartiles of LINE-1 methylation were 1.1 (95% confidence interval (CI) 0.7–1.8), 1.4 (95% CI 0.8–2.2), and 2.6 (95% CI 1.7–4.1) (P for trend <0.001), respectively, compared to individuals in the fourth (highest) quartile. A 1.9-fold (95% CI 1.4–2.6) increased risk of HCC was observed among individuals with LINE-1 methylation below the median compared to individuals with higher (>median) LINE-1 methylation. Our results demonstrate for the first time that individuals with global hypomethylation measured in LINE-1 repeats in blood leukocyte DNA have an increased risk for HCC. Our data provide the evidence that global hypomethylation detected in the easily obtainable DNA source of blood leukocytes may help identify individuals at risk of HCC.  相似文献   

马克思主义中国化"历史路标"的探索     

顾海良 《思想理论教育导刊》2011,(6)

马克思主义中国化的"历史路标",展示的是中国化马克思主义形成和发展的历史过程、历史阶段及其规律性.通过对这一"历史路标"探索,能够更为全面地认识马克思主义中国化的历史发展与社会现实的关系,深刻把握中国化马克思主义的国情基础和时代蕴意;认识马克思主义基本原理与中国具体实际结合的路径与脉络,深刻领悟中国化马克思主义的精神实质和主要特征;认识马克思主义中国化第二次历史性飞跃的战略思想,深刻把握中国特色社会主义理论体系的重大理论问题和主要理论形态的内在逻辑和基本内涵;认识马克思主义中国化的历史发展与理论创新的关系,深刻理解马克思主义中国化、时代化、大众化的显著特性和根本要求.  相似文献   

上海市高校高水平足球队建设与管理探索          下载免费PDF全文

谷腾飞  姜丽萍 《上海教育评估研究》2019,8(4):5-9

高校高水平运动队是我国体育事业的重要组成部分,准确评估是促进发展的基础。文章对上海市高校高水平足球队的基本情况、获奖情况、建设情况、管理情况、训练情况等进行了分析,总结了上海市高校高水平足球队建设的成效与问题,并提出了相应的建议,以期为上海市高校高水平足球队的发展提供参考。  相似文献   

授权型领导影响知识型员工创新的信任机制   总被引：1，自引：0，他引：1  

魏华飞  古继宝  张淑林 《科研管理》2020,41(4):103-111

授权型领导是领导对员工信任基础上所作出的管理决策和领导方式,并影响到员工创新行为。本文研究授权型领导如何通过影响知识型员工的两种信任,并进一步影响知识型员工创新行为的中间信任机制,以及组织支持感在该机制中的调节作用。通过配对调研和数据收集与处理,实证研究发现,授权型领导能够正向影响知识型员工对领导产生的基于情感的信任和基于认知的信任,并最终正向影响知识型员工的创新行为,组织支持感正向调节授权型领导对知识型员工基于情感的信任和基于认知的信任的影响关系。  相似文献   

“旋转木马教学法”在药理学中的应用——以“高血压的临床用药”为例     

吴虹  顾宏霞  许应生 《科教文汇》2020,(10):95-97

本文将“旋转木马教学法”运用到药理学的教学中,以高血压的临床用药为例,教师创设连锁药店职场情境,学生按照“旋转木马”教学原则实施高血压“问病荐药”角色扮演,融入企业文化和元素,明确课程主题,在真实的职场环境中实施教学,激发学生的学习兴趣,培养学生的职业素养。  相似文献   

浅谈如何适应高中到大学的学习转变——以攻读大学生物学专业为例     

顾宇杰 《科教文汇》2020,(13):62-63

自2016年高考改革以来,已经有三届考生通过新高考进入大学。随着高考体制的不断变革与完善,各学科考试的新颖度与难度也在逐年增加,作为亲历新高考的考生,在此文中一方面是想谈谈近年来高考体制的具体变化;另一方面,则是结合个人在大学学习中的心得,为即将面对高考的考生们提供一些建议,以便考生能更好地适应高中至大学的转变。  相似文献   

Steering elementary steps towards efficient alkaline hydrogen evolution via size-dependent Ni/NiO nanoscale heterosurfaces     

Lu Zhao  Yun Zhang  Zhonglong Zhao  Qing-Hua Zhang  Lin-Bo Huang  Lin Gu  Gang Lu  Jin-Song Hu  Li-Jun Wan 《国家科学评论(英文版)》2020,7(1):27

Alkaline hydrogen evolution reaction (HER), consisting of Volmer and Heyrovsky/Tafel steps, requires extra energy for water dissociation, leading to more sluggish kinetics than acidic HER. Despite the advances in electrocatalysts, how to combine active sites to synergistically promote both steps and understand the underlying mechanism remain largely unexplored. Here, Density Functional Theory (DFT) calculations predict that NiO accelerates the Volmer step while metallic Ni facilitates the Heyrovsky/Tafel step. A facile strategy is thus developed to control Ni/NiO heterosurfaces in uniform and well-dispersed Ni-based nanocrystals, targeting both reaction steps synergistically. By systematically modulating the surface composition, we find that steering the elementary steps through tuning the Ni/NiO ratio can significantly enhance alkaline HER activity, and Ni/NiO nanocrystals with a Ni/NiO ratio of 23.7% deliver the best activity, outperforming other state-of-the-art analogues. The results suggest that integrating bicomponent active sites for elementary steps is effective for promoting alkaline HER, but they have to be balanced.  相似文献   

MKRN3 regulates the epigenetic switch of mammalian puberty via ubiquitination of MBD3     

Chuanyin Li  Wenli Lu  Liguang Yang  Zhengwei Li  Xiaoyi Zhou  Rong Guo  Junqi Wang  Zhebao Wu  Zhiya Dong  Guang Ning  Yujiang Shi  Yinmin Gu  Peng Chen  Zijian Hao  Tianting Han  Meiqiang Yang  Wei Wang  Xuehui Huang  Yixue Li  Shan Gao  Ronggui Hu 《国家科学评论(英文版)》2020,7(3):671

Central precocious puberty (CPP) refers to a human syndrome of early puberty initiation with characteristic increase in hypothalamic production and release of gonadotropin-releasing hormone (GnRH). Previously, loss-of-function mutations in human MKRN3, encoding a putative E3 ubiquitin ligase, were found to contribute to about 30% of cases of familial CPP. MKRN3 was thereby suggested to serve as a ‘brake’ of mammalian puberty onset, but the underlying mechanisms remain as yet unknown. Here, we report that genetic ablation of Mkrn3 did accelerate mouse puberty onset with increased production of hypothalamic GnRH1. MKRN3 interacts with and ubiquitinates MBD3, which epigenetically silences GNRH1 through disrupting the MBD3 binding to the GNRH1 promoter and recruitment of DNA demethylase TET2. Our findings have thus delineated a molecular mechanism through which the MKRN3–MBD3 axis controls the epigenetic switch in the onset of mammalian puberty.  相似文献   

Drone-based entanglement distribution towards mobile quantum networks     

Hua-Ying Liu  Xiao-Hui Tian  Changsheng Gu  Pengfei Fan  Xin Ni  Ran Yang  Ji-Ning Zhang  Mingzhe Hu  Jian Guo  Xun Cao  Xiaopeng Hu  Gang Zhao  Yan-Qing Lu  Yan-Xiao Gong  Zhenda Xie  Shi-Ning Zhu 《国家科学评论(英文版)》2020,7(5):921

Satellites have shown free-space quantum-communication ability; however, they are orbit-limited from full-time all-location coverage. Meanwhile, practical quantum networks require satellite constellations, which are complicated and expensive, whereas the airborne mobile quantum communication may be a practical alternative to offering full-time all-location multi-weather coverage in a cost-effective way. Here, we demonstrate the first mobile entanglement distribution based on drones, realizing multi-weather operation including daytime and rainy nights, with a Clauser-Horne-Shimony-Holt S-parameter measured to be 2.41 ± 0.14 and 2.49 ± 0.06, respectively. Such a system shows unparalleled mobility, flexibility and reconfigurability compared to the existing satellite and fiber-based quantum communication, and reveals its potential to establish a multinode quantum network, with a scalable design using symmetrical lens diameter and single-mode-fiber coupling. All key technologies have been developed to pack quantum nodes into lightweight mobile platforms for local-area coverage, and arouse further technical improvements to establish wide-area quantum networks with high-altitude mobile communication.  相似文献   

“985工程”院校图书馆电子资源揭示和组织的现状调查     

韩玺  袭继红  顾萍 《图书馆学研究》2011,(12):51-54

对39所＂985工程＂院校图书馆网站进行调查,从是否有OPAC编目揭示、电子资源揭示的方式和种数、图书馆首页资源揭示的方式和种数、是否有使用指南及指南方式、是否提供统一检索以及统一检索平台的类型等五方面获取了图书馆电子资源组织和揭示的情况,并提出了相关建议,为国内其他图书馆科学合理地组织和揭示电子信息资源提供参考。  相似文献