Evaluation of immunohistochemistry and enzyme linked immunosorbent assay for HER-2/neu expression in breast carcinoma     

Rani James  K. Thriveni  Girija Ramaswamy  Lakshmi Krishnamoorthy  Geetashree Mukherjee  P. P. Vijayalaxmi Deshmane  P. P. Bapsy 《Indian journal of clinical biochemistry : IJCB》2008,23(4):345-351

HER-2 is overexpressed in approximately 20–30% of invasive Breast Cancer. ECD of the HER-2 protein is frequently cleaved and released into the circulation, where it can be detected by ELISA in up to 45% of patients with metastatic breast cancer. The objective of our study was to compare the current methods for the detection of HER-2 protein. Tissue HER-2 levels were studied in 100 breast cancer patients by IHC and compared with serum HER-2 levels by ELISA. IHC frequency was 29%. Serum HER-2 ECD was positive in 42% of patients. A statistically significant correlation was observed. HER-2 detected by IHC correlates significantly with serum HER-2 levels detected by ELISA. Thus, ELISA is a reliable and economical tool to assess the HER-2 status in tumors, when breast tissue sample is not available.  相似文献   

SRB显色法用于抗癌药物敏感性试验的研究   总被引：13，自引：0，他引：13  

王青青  余海 《科技通报》2000,16(2):104-107

采用ＳＲＢ显色法对９６孔培养板中短期培养的人大肠癌细胞株Ｃｏｌｏ２０５、人骨肉瘤细胞株ＯＳ７３２,人舌癌细胞株Ｔｃａ８１１３经５－ＦＵ,顺铂,蟾酥等药物处理后的活性进行了研究。结果表明,ＳＲＢ法对这些细胞株的体外抗癌药物敏感试验结果稳定,该法与其它细胞毒试验相比有一定优点,值得推广应用。  相似文献   

Hyperinsulinemia and Hypoadiponectinemia are Associated with Increased Risk for Occurrence of Ovarian Cancer in Non-diabetic Women of North Indian Population     

Rohit Kumar Gupta  Sagar Jayantilal Dholariya  Smita Kaushik  S. K. Gupta  Reva Tripathi  Shyam Lata Jain 《Indian journal of clinical biochemistry : IJCB》2021,36(2):221

Ovarian cancer has been emerged as a most common and lethal gynecological malignancy in India. High serum insulin and low adiponectin have been associated with increased risk of ovarian cancer. But their role in development of ovarian cancer is conflicting and little evidence is available. We aimed to evaluate blood levels of insulin and adiponectin in epithelial ovarian cancer (EOC) patients and their association with the risk to develop EOC. The study included following three groups; Group 1: fifty cases of cytohistopathologically confirmed cases of EOC, Group 2: fifty age matched cases of benign ovarian conditions and Group 3: fifty ages matched healthy controls with no evidence of any benign or malignant ovarian pathology as ruled out by clinical examination and relevant investigations. Cytohistopathologically confirmed and newly diagnosed cases of EOC and benign ovarian cancer were included in this study. The median value of fasting serum insulin was significantly high (15.0 µlU/ml, P = 0.02) and adiponectin were significantly low (5.1 µg/ml, P < 0.001) in ovarian cancer patients compared to benign ovarian tumors and healthy controls group. A significant increase risk of ovarian cancer was found in high tertile (≥ 18.7 µlU/ml) of serum insulin level (OR = 2.7; 95% CI = 1.00–6.67, P = 0.04) and lower tertile (≤ 5.45 µg/ml) of adiponectin level (OR = 3.2; 95% CI = 1.10–9.71, P = 0.03). High serum insulin level and low adiponectin levels were significantly associated with increased risk for development of ovarian cancer.  相似文献   

抗癌食品与防癌机理综述     

白文钊 《安康学院学报》2002,14(3):69-71

癌症是人类的一大疾患,严重地威胁着人体生命,对于癌症的发病机理、成因、预防措施、诊治技术等方面的研究是目前全世界十分关注的重大课题.对抗癌食品以及抗癌的原理和方法等进行论述.  相似文献   

国内有关硒与癌症发生相关文献的评价          下载免费PDF全文

王雪梅  刘莉  黄碧云 《情报工程》2016,2(1):008-014

目的:通过对国内有关硒与癌症发生的相关文献进行循证医学评价来了解目前相关文献的质量现状.方法:在生物医学文献数据库中以"硒"和"癌症"为主题词检索建库至2014年6月的相关文献,对检出的文献进行筛选后,利用九级证据评价标准及M量表对所纳入的文献进行评价.结果:九级评价标准:纳入的376篇文献中系统评价/Meta分析的文献为0篇,随机对照试验(RCT)文献33篇,其他343篇.M量表:33篇RCT文献评价结果中4分的文献有3篇,未见大于4分的文献.结论:九级评价结果:目前国内有关硒与癌症关系的研究中,动物研究和体外研究占多数,RCT研究相对较少,系统评价/Meta分析文献未见.M量表评价结果:目前国内有关硒与癌症发生相关文献的质量整体水平不高,在实验研究设计中存在很多问题,有待进一步提高.高质量证据的研究表明硒具有防癌治癌的作用,硒制剂能够增强一些化疗药物的疗效,并且减少化疗或放疗对人体产生的副作用.  相似文献   

热休克蛋白(HSP70、HSP27)在食管癌组织中表达的意义     

张世棋  马春梅  蒋丽娜  薄爱华  张晓丽  邢立强 《河北北方学院学报(医学版)》2002,19(2):56-57

目的 :探讨HSP70和HSP2 7在食管癌组织中的表达情况。方法 :采用SP免疫组织化学染色检测 5 9例食管癌组织中HSP70和HSP2 7。结果 :HSP70和HSP2 7的总阳性率分别为 6 4.4 1% (38/5 9)和 5 8.6 2 % (34/5 8) ,HSP70和HSP2 7的共同阳性率为 30 .0 0 % (9/30 )。结论 :在食管癌中HSP70和HSP2 7普遍表达。  相似文献   

机场航站楼桩基基础施工技术探讨     

王长津 《延安职业技术学院学报》2019,(2):106-108

桩基基础施工技术种类繁多,常见的木桩、钢桩、钢筋混凝土桩等。在机场航站楼施工中绝大部分为钢筋混凝土桩,结合贵州省贵阳市龙洞堡国际机场1号航站楼扩建工程2标段实际情况,分别从桩基人工开挖,机械开挖等方面对机场航站楼桩基基础施工技术探讨。研究表明:机场航站楼桩基础施工技术,施工更加稳定,安全性高,施工工期更加容易掌控,值得大范围推广应用。  相似文献   

Oxidative stress and antioxidant status in cervical cancer patients     

M. Smita K. Naidu  A. N. Suryakar  Sanjay C. Swami  R. V. Katkam  K. M. Kumbar 《Indian journal of clinical biochemistry : IJCB》2007,22(2):140-144

Cervical cancer (CaCx) is a global public health problem as it is the second most common cancer leading to the death of women worldwide. Many references revealed that the low levels of antioxidants induce the generation of free radicals leading to DNA damage and further mutations. In the present study attempt have been made to evaluate the levels of serum Lipid peroxide, Nitric Oxide (NO^.) Erythrocytic—Superoxide Dismutase (RBC-SOD), Vitamin-C, serum Copper (Cu) and serum Zinc (Zn). 120 patients were divided in 4 groups according to the increasing CaCx stages i.e. stage I, II, III & IV respectively. All the patients were around the age group of 25–65 years. 30 healthy women between the same age group were treated as controls. Highly significant increased values of MDA, NO^. and Cu were observed (p<0.001) whereas the activity of RBC-SOD, levels of Vitamin-C and Zn were significantly decreased in CaCx patients as compared with healthy controls (p<0.001). Cu/Zn ratio was found to be altered in CaCx patients. From our findings it can be concluded that the oxidative stress is induced among CaCx patients, which inturn increases the risk of CaCx.  相似文献   

Association between physical activity and digestive-system cancer: An updated systematic review and meta-analysis     

Fangfang Xie  Yanli You  Jihan Huang  Chong Guan  Ziji Chen  Min Fang  Fei Yao  Jia Han 《运动与健康科学(英文)》2021,10(1):4

BackgroundPhysical activity (PA) may have an impact on digestive-system cancer (DSC) by improving insulin sensitivity and anticancer immune function and by reducing the exposure of the digestive tract to carcinogens by stimulating gastrointestinal motility, thus reducing transit time. The current study aimed to determine the effect of PA on different types of DSC via a systematic review and meta-analysis.MethodsIn accordance with the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines, we searched for relevant studies in PubMed, Embase, Web of Science, Cochrane Library, and China National Knowledge Infrastructure. Using a random effects model, the relationship between PA and different types of DSC was analyzed.ResultsThe data used for meta-analysis were derived from 161 risk estimates in 47 studies involving 5,797,768 participants and 55,162 cases. We assessed the pooled associations between high vs. low PA levels and the risk of DSC (risk ratio (RR)  = 0.82, 95% confidence interval (95%CI): 0.79–0.85), colon cancer (RR = 0.81, 95%CI: 0.76–0.87), rectal cancer (RR = 0.88, 95%CI: 0.80–0.98), colorectal cancer (RR = 0.77, 95%CI: 0.69–0.85), gallbladder cancer (RR = 0.79, 95%CI: 0.64–0.98), gastric cancer (RR = 0.83, 95%CI: 0.76–0.91), liver cancer (RR = 0.73, 0.60–0.89), oropharyngeal cancer (RR = 0.79, 95%CI: 0.72–0.87), and pancreatic cancer (RR = 0.85, 95%CI: 0.78–0.93). The findings were comparable between case-control studies (RR = 0.73, 95%CI: 0.68–0.78) and prospective cohort studies (RR = 0.88, 95%CI: 0.80–0.91). The meta-analysis of 9 studies reporting low, moderate, and high PA levels, with 17 risk estimates, showed that compared to low PA, moderate PA may also reduce the risk of DSC (RR = 0.89, 95%CI: 0.80–1.00), while compared to moderate PA, high PA seemed to slightly increase the risk of DSC, although the results were not statistically significant (RR = 1.11, 95%CI: 0.94–1.32). In addition, limited evidence from 5 studies suggested that meeting the international PA guidelines might not significantly reduce the risk of DSC (RR = 0.96, 95%CI: 0.91–1.02).ConclusionCompared to previous research, this systematic review has provided more comprehensive information about the inverse relationship between PA and DSC risk. The updated evidence from the current meta-analysis indicates that a moderate-to-high PA level is a common protective factor that can significantly lower the overall risk of DSC. However, the reduction rate for specific cancers may vary. In addition, limited evidence suggests that meeting the international PA guidelines might not significantly reduce the risk of DSC. Thus, future studies must be conducted to determine the optimal dosage, frequency, intensity, and duration of PA required to reduce DSC risk effectively.  相似文献   

Gene expression and apoptosis response in hepatocellular carcinoma cells induced by biocompatible polymer/magnetic nanoparticles containing 5-fluorouracil     

《Electronic Journal of Biotechnology》2021

BackgroundSuper-paramagnetic iron oxide nanoparticles (SPION) contain a chemotherapeutic drug and are regarded as a promising technique for improving targeted delivery into cancer cells.ResultsIn this study, the fabrication of 5-fluorouracil (5-FU) was investigated with loaded Dextran (DEX-SPION) using the co-precipitation technique and conjugated by folate (FA). These nanoparticles (NPs) were employed as carriers and anticancer compounds against liver cancer cells in vitro. Structural, magnetic, morphological characterization, size, and drug loading activities of the obtained FA-DEX-5-FU-SPION NPs were checked using FTIR, VSM, FESEM, TEM, DLS, and zeta potential techniques. The cellular toxicity effect of FA-DEX-5-FU-SPION NPs was evaluated using the MTT test on liver cancer (SNU-423) and healthy cells (LO2). Furthermore, the apoptosis measurement and the expression levels of NF-1, Her-2/neu, c-Raf-1, and Wnt-1 genes were evaluated post-treatment using flow cytometry and RT-PCR, respectively. The obtained NPs were spherical with a suitable dispersity without noticeable aggregation. The size of the NPs, polydispersity, and zeta were 74 ± 13 nm, 0.080 and −45 mV, respectively. The results of the encapsulation efficiency of the nano-compound showed highly colloidal stability and proper drug maintenance. The results indicated that FA-DEX-5-FU-SPION demonstrated a sustained release profile of 5-FU in both phosphate and citrate buffer solutions separately, with higher cytotoxicity against SNU-423 cells than against other cells types. These findings suggest that FA-DEX-SPION NPs exert synergistic effects for targeting intracellular delivery of 5-FU, apoptosis induction, and gene expression stimulation.ConclusionsThe findings proved that FA-DEX-5-FU-SPION presented remarkable antitumor properties; no adverse subsequences were revealed against normal cells.How to cite: Mahdia SA, Kadhimb AA, Albukhaty S, et al. Gene expression and apoptosis response in hepatocellular carcinoma cells induced by biocompatible polymer/magnetic nanoparticles containing 5-fluorouracil. Electron J Biotechnol 2021;52. https://doi.org/10.1016/j.ejbt.2021.04.001  相似文献