Evaluation and application of an efficient plant DNA extraction protocol for laboratory and field testing     

Qi WANG  Xiaoxia SHEN  Tian QIU  Wei WU  Lin LI  Zhi'an WANG  Huixia SHOU 《Journal of Zhejiang University. Science. B》2021,22(2):99

Nucleic acids in plant tissue lysates can be captured quickly by a cellulose filter paper and prepared for amplification after a quick purification.In this study,a published filter paper strip method was modified by sticking the filter paper on a polyvinyl chloride resin(PVC)sheet.This modified method is named EZ-D,for EASY DNA extraction.Compared with the original cetyl trimethylammonium bromide(CTAB)method,DNA extracted by EZ-D is more efficient in polymerase chain reaction(PCR)amplification due to the more stable performance of the EZ-D stick.The EZ-D method is also faster,easier,and cheaper.PCR analyses showed that DNA extracted from several types of plant tissues by EZ-D was appropriate for specific identification of biological samples.A regular PCR reaction can detect the EZ-D-extracted DNA template at concentration as low as 0.1 ng/μL.Evaluation of the EZ-D showed that DNA extracts could be successfully amplified by PCR reaction for DNA fragments up to 3000 bp in length and up to 80%in GC content.EZ-D was successfully used for DNA extraction from a variety of plant species and plant tissues.Moreover,when EZ-D was combined with the loop-mediated isothermal amplification(LAMP)method,DNA identification of biological samples could be achieved without the need for specialized equipment.As an optimized DNA purification method,EZ-D shows great advantages in application and can be used widely in laboratories where equipment is limited and rapid results are required.  相似文献   

河南农民收入增长的波动周期   总被引：6，自引：1，他引：5  

刘荣利 《四川教育学院学报》2008,24(11):37-39

河南农民人均收入时间序列,服从二阶单整过程,期间存在结构性断点,适合建立H—P滤波模型。自改革开放以来可划分为三个增长周期,其在不同的历史阶段具有明显不同的波动特征。  相似文献   

宽阻带小型化低通滤波器的设计     

杨雪霞  薛玉杰 《上海大学学报(英文版)》2008,12(6):495-497

This paper presents a novel low-pass filter （LPF） with sharp rejection, wide stopband and compact size, which are realized by the defected ground structure （DGS） and the defected microstrip structure （DMS）. The equivalent circuit model is proposed and the circuit parameters are extracted by the circuit simulation software. The parameters measured are 3 dB cutoff frequency fc of 5.2 GHz, the insertion loss less than 0.5 dB from DC to 4.0 GHz and S21 less than -20 dB within the wide stopband from 6 GHz to 16 GHz. The results of the circuit optimization agree well with those of the full wave simulation and the measured ones, which validate the effectiveness of the equivalent circuit model. The size of the proposed LPF is decreased compared with normal LPF. This LPF can be applied in rectennas to eliminate high order harmonics.  相似文献   

自适应信号处理技术在IP电话技术中的应用探讨     

曾素琼 《嘉应学院学报》2001,19(6):63-66

本文简要介绍了自适应系统结构、特征,阐述了目前IP电话技术及存在问题,主要对利用自适应信号处理技术来解决IP电话技术系统中的回声问题作了一些探讨。  相似文献   

一种结合深度特征的人体运动序列追踪模型     

蒋宇  袁健 《教育技术导刊》2020,19(1):89-94

目前主流的判别式目标跟踪模型大多使用灰度、颜色等手工特征,在目标快速移动或受到视频序列背景等因素干扰情况下,目标跟踪器可能在跟踪目标时学习到错误特征而导致跟踪失败。因此,提出一种结合深度特征的相关滤波跟踪算法。首先将待跟踪目标图像输入至卷积神经网络中,提取出较高层的卷积特征,然后将提取的卷积特征输入相关滤波器中得到响应,最后根据响应峰值得到追踪结果。以VOT2016中包含人体运动的视频序列为实验数据集,并分别与CN、SAMF及KPDCF模型进行对比。实验结果表明,结合深度特征的相关滤波算法具有较好的追踪性能,在不大幅降低追踪速度的情况下,提升了追踪精度和稳定性。  相似文献   

一种滤除基线漂移和工频干扰的数字滤波算法   总被引：1，自引：0，他引：1  

邹波  周远 《深圳职业技术学院学报》2005,4(4):3-5

介绍了一种用于心电监护的数字滤波算法，对滤波器的设计，包括滤波原理、整系数数字滤波器的波型特征、实现方框图及10点平均数字滤波器进行了分析，并给出心电信号的滤波效果图。实验结果表明，该算法能有效滤除基线漂移和工频干扰，算法简单实用。  相似文献   

An adaptive fuzzy filter for coding artifacts removal in video and image     

WU Jing YE Xiu-qing GU Wei-kang 《浙江大学学报(A卷英文版)》2007,8(6):841-848

This paper proposes a new adaptive post-filtering algorithm to remove coding artifacts in block-based video coder. The proposed method concentrates on blocking and ringing artifacts removal. For de-blocking, the blocking strength is identified to determine the filtering range, and the maximum quantization parameter of the image is used to adapt the 1D fuzzy filter. For de-ringing, besides the edge detection, a complementary ringing detection method is proposed to locate the neglected ringing blocks, and the gradient threshold is adopted to adjust the parameter of 2D fuzzy filter. Experiments are performed on the MPEG-4 sequences. Compared with other methods, the proposed one achieves better detail preservation and artifacts removal performance with lower computational cost.  相似文献   

论文化传播中的文学误读及意义          下载免费PDF全文

张利群 《惠州学院学报》2001,21(3):47-53

文化传播中因文化过滤的原因往往会造成文化误读,它是因为发送者文化损耗和接受者文化渗透所造成的。文学在文化传播中的误读就更为明显,既有“见仁见智”的积极性误读,又有理解偏差而造成的消极性误读。但从本质而言,都应是阅读者的积极主动阅读所致,都在一定程度上体现出批评的主体性,具有再创造和文化过滤的意义。  相似文献   

采用改进电流传送器的二阶电流滤波器     

黄满池  彭良玉 《湖南师范大学教育科学学报》1997,(2)

本文提出了一种实现二阶和一阶电流滤波的新电路，该电路由一个改进的第二代电流传送器（MCCⅡ）和少量的无源元件组成．能实现二阶低通、高通、带通、全通（陷波）和一阶低通、高通、全通电流滤波．这种电路很容易级联而不需附加匹配装置．而且还具有低的无源和有源灵敏度．  相似文献   

扇形滤纸的一种新的折叠方法     

田宗明 《宁波职业技术学院学报》2005,9(5):86-88

介绍了扇形滤纸的一种新的折叠方法,并与常用的折叠方法进行了比较.结果表明,这种新的折叠方法具有折叠简单、易懂易学的优点,且过滤效果好,是一种值得普及推广的新方法.  相似文献