尿嘧啶DNA糖苷酶活性的电泳凝胶成像分析测定     

陈朝银  刘丽  贲昆龙 《科技通报》2004,20(2):116-120

尿嘧啶DNA糖苷酶(UNG)是防止PCR假阳性反应的工具酶,参照限制性DNA内切酶活力测定法的原理(以完全消化DNA带型的最高稀释度为一个活性单位)和应用凝胶成像分析技术,建立了UNG酶活性的非同位素检测新方法.反应体积为20μL,底物dU ung(dU DNA)为165μg,加UNG后在适当温度下反应10min,使底物去除尿嘧啶,其AP位点经碱降解.通过琼脂糖凝胶电泳和凝胶成像分析,测定底物dU ung的残留量以测定UNG酶活性.酶活力以37℃每分钟降解1ngdU ung的酶量为一个单位,检测灵敏度为1ng,检测线性范围为0～165ng.该法简便易行、客观、精密,重复性好(CV<3%),结果显示直观.  相似文献   

Science Letters：Alterations in seedling vigour and antioxidant enzyme activities in Catharanthus roseus under seed priming with native diazotrophs     

Karthikeyan B  Jaleel CA  Gopi R  Deiveekasundaram M 《Journal of Zhejiang University. Science. B》2007,8(7):453-457

An experiment was conducted on Catharanthus roseus to study the effect of seed treatments with native diazotrophs on its seedling growth and antioxidant enzyme activities. The treatments had significant influence on various seedling parameters. There is no significant influence on dry matter production with the diazotrophs, Azospirillum and Azotobacter. However, the vital seedling parameters such as germination percentage and vigour index were improved. Azotobacter treatment influenced maximum of 50% germination, whereas Azospirillum and Azotobacter were on par with C. roseus with respect to their vigour index. There was significant difference in the population of total diazotrophs. Azospirillum and Azotobacter between rhizosphere and non-rhizosphere soils of C. roseus had the same trend and were observed at various locations of the study. The activities of antioxidant enzymes such as superoxide dismutase （SOD）, peroxidase （POX） and catalase （CAT） were increased to a significant extent due to the treatment with diazotrophs.  相似文献   

Correlation of serum arylesterase activity on phenylacetate estimated by the integrated method to common classical biochemical indexes of liver damage     

Liao F  Zhu XY  Wang YM  Zhao YS  Zhu LP  Zuo YP 《Journal of Zhejiang University. Science. B》2007,8(4):237-241

The correlation of serum arylesterase (PON1) activity on phenylacetate determined by an integrated method to classical biochemical indexes of liver damage was investigated for the use of PON1 activity to evaluate liver damage. PON1 reaction curve as absorbance at 270 nm for 0.20 mmol/L phenylacetate hydrolysis was analyzed by the integrated method to determine maximal PON1 reaction rate. Classical biochemical indexes of liver damage were determined routinely. The 95% confidence threshold of PON1 activity in sera from healthy individuals was 2.12 mkat/L [(4.73±1.31) mkat/L, n=105]. PON1 activity in clinical sera was closely correlated to serum albumin, total protein and the ratio of albumin to globulins, but was weakly correlated to both direct and total bilirubin in serum. There were no correlations of PON1 activity to γ-glutamyltransferase, alkaline phosphatase, alanine aminotransferase and aspartate aminotransferase. Among 127 clinical sera with PON1 activity＞2.12 mkat/L, there were 92% healthy individuals examined by albumin, 90% healthy individuals examined by total protein, 88% healthy individuals examined by total bilirubin, 86% healthy individuals examined by direct bilirubin and 64% healthy individuals examined by the ratio of albumin to globulins, respectively. In each group of healthy individuals judged by classical biochemical indexes of close correlation to PON1 activity, percentage of healthy individuals examined by PON1 activity was always ＞80%. These results suggested PON1 activity on phenylacetate estimated by the integrated method was also suitable for the evaluation of liver damage.  相似文献   

不同植被恢复模式对石漠化地区土壤多酚氧化酶的影响     

覃勇荣  韦丹妮  黄健  岑忠用  覃宝山  蓝崇钰 《河池学院学报》2007,27(2):41-45

多酚氧化酶是一种重要的土壤酶,对土壤环境的变化比较敏感,可作为土壤肥力变化和生态恢复的监测指标。为了深入了解桂西北石漠化地区生态恢复的现状,作者对广西宜州、平果等地进行了实地调查和取样,并从不同植被恢复的角度,对桂西北石漠化地区封山育林、吊丝竹(D.minor(McClure) Chia et H.L.Fung)、任豆(Zenia insignis Chun)等不同植被恢复模式样地的土壤多酚氧化酶及其与土壤有机质、全N、全P、有效N、有效P的相关性等方面进行了比较分析,试图了解不同植被恢复模式对石漠化地区生态恢复过程中土壤多酚氧化酶活性的影响,结果表明,以豆科植物为优势种或与豆科植物混交的植被恢复模式,其土壤多酚氧化酶活性较高,其他相关的土壤肥力测试指标也较高,因此,可以将之作为石漠化土地植被恢复的优化模式。  相似文献   

淀粉酶对麦芽糖和蔗糖的作用及酶的专一性   总被引：1，自引：0，他引：1  

娄志义 《生物学教学》2005,30(6):64-64

作为生物体内的催化剂，酶具有高效性、专一性以及易受温度和pH的影响等特点，教材中针对以上的特点安排了相关实验加以验证。其中“探索淀粉酶对淀粉和蔗糖的作用”的实验显示：淀粉酶只能催化淀粉的水解，而对蔗糖不起作用，说明“酶的催化作用具有专一性”的特点。而在高三选修课本中，从“探索温度对淀粉酶活性的影响”的实验原理中我们知道。淀粉酶能催化淀粉水解为麦芽糖和葡萄糖。  相似文献   

盐胁迫对鱼腥草几种膜保护酶活性的影响     

《怀化学院学报》2017,(5)

以峨眉蕺菜和湘白鱼腥草为材料,研究了NaCl胁迫对其膜脂过氧化和膜保护酶系统的影响.结果表明:随着NaCl浓度的增加,两个品种MDA含量呈现明显上升趋势;SOD、POD、CAT活性呈现先上升后下降的趋势;两者地下茎、地上茎、叶的伤害率呈上升趋势.鱼腥草受到盐胁迫时,最先受到影响的是叶片,其次是地上茎,再次是地下茎.峨眉蕺菜的膜系统耐盐胁迫的能力较强,膜脂过氧化的产物含量比较低,而湘白鱼腥草对盐胁迫反应较快,但波动较大,膜系统耐盐胁迫的能力较弱.  相似文献   

种子劣变机制研究进展   总被引：3，自引：0，他引：3  

张兆英 《沧州师专学报》2005,21(2):89-90

种子劣变是种子内部一系列生理生化反应的结果.主要表现在:细胞膜完整性的丧失和脂质过氧化程度的加深,各种酶类活性的降低,呼吸协调的不断破坏,种子内蛋白质、脂肪等贮藏物质的变化以及赤霉素、脱落酸等激素含量的变化等方面.  相似文献   

探究"影响酶活性的条件"的两个"意外"     

黄广慧 《生物学教学》2006,31(1):47-48

人教版高中生物课程标准实验教科书中增设了不少的探究活动，这些探究活动需要学生自行制订实验方案，具有很大的开放性，难免会出现一些意外现象。对此，如果教师能给予适当引导，将会有意想不到的教学价值。本文列举两例学生在探究“影响酶活性的条件”时的意外发现。  相似文献   

大气污染对几种植物过氧化物酶活性的影响     

赵素达 《青岛职业技术学院学报》2000,(2)

研究了青岛市大气重污染地区楼山后一些植物 (油菜、菠菜、云豆、土豆、菜花等 )过氧化物酶活性的变化 ,指出该地区植物叶子内过氧化物酶活性普遍较高 ,其原因是大气污染造成的。大气中的主要污染物是SO2 ,过氧化物酶活性的变化可以作为监测大气污染对植物造成危害的生理指标。  相似文献   

pH值对昆明裂腹鱼淀粉酶活性的影响   总被引：2，自引：3，他引：2  

胡思玉  白建梅  葛传龙  王延斌  陈永祥 《毕节学院学报》2006,24(4):72-74

在最适pH值条件下,昆明裂腹鱼胃、前肠、中肠、后肠和肝等主要消化器官淀粉酶活性分别为669.5、789.23、796.86、761.51和774.09,淀粉酶活性最高的是中肠,最低的是胃。其最适pH在7.4到8.6之间。  相似文献