Vitamin C supplementation does not influence plasma and blood mononuclear cell IL-6 and IL-10 levels after exercise     

Antoni Aguiló  Marta Monjo  Carlos Moreno  Pau Martinez  Sonia Martínez 《Journal of sports sciences》2013,31(17):1659-1669

Abstract

The aim of this study was to determine whether the highest vitamin C supplementation associated with complete bioavailability influences the plasma and blood mononuclear cell IL-6 and IL-10 response to exercise. A double-blinded study of supplementation with vitamin C was performed. After 15 days of supplementation with vitamin C (500 mg · day^?1, n = 16) or a placebo (n = 15), participants in the study completed a 15-km run competition. Blood samples were taken before and after competition. Oxidative stress markers, antioxidants, cortisol, IL-6 and IL-10 were determined in plasma or serum. IL-6 and IL-10 protein and mRNA levels were measured in blood mononuclear cells. Although higher plasma and blood mononuclear cell vitamin C levels were observed in the supplemented group when compared with the placebo one, the two groups showed identical exercise-induced changes in all the measured parameters. Exercise induced increased IL-6 and IL-10 levels in plasma and blood mononuclear cells. IL-6 and IL-10 mRNA levels in blood mononuclear cells increased after the competition. After recovery, IL-6 mRNA returned to basal levels and IL-10 mRNA levels remained elevated. In conclusion, exercise induced increased IL-6 and IL-10 production in blood mononuclear cells. However, vitamin C supplementation did not influence IL-6 and IL-10 response to exercise.  相似文献   

The microcycle of inflammation and performance changes after a basketball match     

Athanasios Chatzinikolaou  Dimitrios Draganidis  Alexandra Avloniti  Alexandros Karipidis  Athanasios Z Jamurtas  Chrysanthi L Skevaki 《Journal of sports sciences》2013,31(9):870-882

Abstract

Basketball incorporates intense eccentric muscle activity that induces muscle microtrauma and an inflammatory response. This study investigated time-dependent inflammatory and performance responses during a weekly microcycle after a basketball match. Twenty elite-standard players underwent a trial that comprised a match followed by a 6-day simulated in-season microcycle. The trial was preceded by a control condition that did not have a match. Blood sampling and tests of maximal-intensity exercise performance and muscle damage occurred before each condition, immediately after the match and daily thereafter for 6 consecutive days. The match induced marked increases in heart rate, lactate, ammonia, glucose, non-esterified fatty acids and triglycerides. Performance deteriorated for 24–48 h after the match, whereas knee flexor and extensor soreness increased for 48 and 24 h post-match, respectively. Inflammatory (leukocytes, C-reactive protein, creatine kinase activity, adhesion molecules, cortisol, uric acid and cytokines) and oxidative stress (malondialdehyde, protein carbonyls, oxidised glutathione, antioxidant capacity, catalase and glutathione peroxidase) markers increased for ~24 h and subsided thereafter. Reduced glutathione declined for 24 h after exercise. These results suggest that a basketball match elicits moderate and relatively brief (~24–48 h) inflammatory responses, is associated with marked but short-lived performance deterioration, but is less stressful than other intermittent-type sports.  相似文献   

Analysis of protein posttranslational modifications by mass spectrometry: With special reference to haemoglobin     

Murali Woodi  Amit Kumar Mondal  Balaram Padmanabhan  Krishnaswamy Patnam Rajagopalan 《Indian journal of clinical biochemistry : IJCB》2009,24(1):23-29

Mass spectrometry provides a convenient platform for the study of different protein post translational modifications from clinical specimen. Analysis of different post translational modifications of hemoglobin like glycation and glutathionylation can provide useful information on the disease progression and the possible outcome of therapies. In the present study, we have addressed post translational modifications of hemoglobin like glutathionylation and glycation in relation to diabetes and chronic renal failure. We found that both alpha and beta chains of human hemoglobin are glycated irrespective of the extent of glycemia as evidenced by a mass increment of 162 Da. The phenomenon of glutathionylation was observed with only the beta globin chain of hemoglobin probably due to the presence of an accessible cysteine residue indicated by a mass increment of 305 Da. Also, the extent of gltuathionylation observed in the CRF patients could correlate with the severity of the oxidative stress owing to renal replacement therapies like dialysis and transplantation.  相似文献   

Reproducibility of measurement techniques used for creatine kinase,interleukin-6 and high-sensitivity C-reactive protein determination over a 48 h period in males and females     

Bryna Chrismas  Lee Taylor  Alexander Smith  Philip Pemberton  Jason Charles Siegler  Adrian Wayne Midgley 《Measurement in physical education and exercise science》2018,22(3):191-199

To examine the reproducibility of three measurement techniques used to determine creatine kinase, interleukin-6 and high-sensitivity C-reactive protein, 50 participants had blood samples taken on two occasions. Fingertip plasma samples were analysed using the Reflotron for CK determination. Venous blood samples collected into serum separator tubes were used for IL-6 and hs-CRP analyses. IL-6 was measured using an enzyme linked immune assay development kit. The hs-CRP was measured by an in-house ELISA method. Dependent t-tests showed no systematic bias between samples. The interdian CV was 20.0% for CK, 15.3% for IL-6 and 44.2% for hs-CRP. The intraclass correlation coefficient was 0.90 for CK, 0.98 for IL-6 and 0.70 for hs-CRP. The 95% limits of agreement were ?69.7 to 63.5 IU/L for CK, ?1.48 to 1.80 pg/ml for IL-6 and ?1.10 to 0.91 µg/L for hs-CRP. The results demonstrate low absolute reproducibility, which may obscure a true experimental effect.  相似文献   

Whole body precooling attenuates the extracellular HSP72, IL-6 and IL-10 responses after an acute bout of running in the heat     

Ben J Lee  Neil D Clarke  Joanne Hankey  Charles D Thake 《Journal of sports sciences》2018,36(4):414-421

  相似文献   

Urinary protein thiols in different grades of proteinuria     

Mungli Prakash  Jeevan K. Shetty  Sambit Dash  Bijay K. Barik  Abhirup Sarkar  Sharanabasappa M. Awanti  Ravindra Prabhu 《Indian journal of clinical biochemistry : IJCB》2008,23(4):404-406

Total thiol status of plasma, especially thiol groups over protein contributes maximum to the plasma antioxidant status of the body. Serum protein thiols were found to be decreased in various disease conditions including chronic renal failure patients. Only few studies determined the levels of urinary protein thiols in disease conditions. The current study was designed to know the levels of urinary protein thiols in patients with different grades of proteinuria. The study was conducted on urine of 40 healthy controls and 61 cases with proteinuria. Based on proteinuria cases were further divided into two groups; group I - microproteinuria (150–300 mg protein/d), 32 cases, group II - frank proteinuria (>300 mg protein/d), 29 cases. Urinary thiol levels were determined by spectrophotometric method using dithionitrobenzoic acid. A significant decrease (p<0.01) in urinary thiol in group I and group II cases was observed in present study and this decrease was associated with proteinuria.  相似文献   

带蒂大网膜包肾术对肾小球硬化大鼠残余肾组织的影响     

董兴刚  安增梅  杨海春 《科技通报》2008,24(3):330-334

为了探讨带蒂大网膜包肾术对肾小球硬化大鼠残余肾组织的影响,采用单侧肾切除加二次注射阿霉素制备肾小球硬化大鼠模型,称取体质量,检测血尿素氮、肌酐等,随机分为模型组、带蒂大网膜包肾术治疗组、正常对照组。应用光学显微镜(包括HE、PAS染色),电镜以观察肾组织病理学改变。带蒂大网膜包肾术可降低血尿素氮;带蒂大网膜包肾术治疗的大鼠肾组织病理改变有明显改善。结论带蒂大网膜包肾术可减轻肾小球硬化大鼠肾损伤。  相似文献   

小儿药物性血尿30例临床分析     

王亚萍  邹潮春 《科技通报》1999,15(6):475-477,478

针对小儿药物性血尿,通过３０例的临床分析,发现头孢拉丁与其它抗生素及解热镇痛药合用时引发血尿占首位,其次是解热镇痛药及非类固醇类抗炎药。肾损害的发生与用药剂量过大、过敏性体质等因素有关,通过治疗的患儿大多数在２～３天内肉眼血尿消失,在２～７天内尿常规恢复正常。  相似文献   

运动对胰岛素抵抗大鼠脂肪组织氧化应激及炎症的影响   总被引：1，自引：1，他引：0  

杨洪涛  李娟  陈巍  李曙刚  高磊  尚宁宁 《西安体育学院学报》2010,27(2)

目的 观察运动对胰岛素抵抗(Insulin Resistance IR)大鼠脂肪组织SOD活性,MDA及TNF-α含量的影响,探讨运动对IR改善的机制.方法 22只雄性SD大鼠随机分为空白对照组6只(CG)、胰岛素抵抗组(IRG)和胰岛素抵抗运动组(EIRG)各8只,采用高脂饲料诱导IR动物模型,EIRG大鼠进行为期10周游泳训练,测定各组大鼠脂肪组织中SOD活性,MDA及TNF-α含量.结果 采用高脂饲料诱导IR模型成功,运动干预后与CG比较,IRG大鼠脂肪组织MDA和TNF-α水平显著升高(P<0.05);与IRG比较,EIRG脂肪组织SOD活性显著提高(P<0.05),MDA和TNF-α水平显著降低(P<0.05);IR指数与MDA,IR指数与TNF-α,MDA与TNF-α均成正相关(r:0.430,P:0.01;r:0.475,P:0.025;r:0.432.P:0.045).结论 运动对大鼠IR的改善机制可能是通过运动上调脂肪组织抗氧化酶活性,进而降低炎性脂肪因子表达实现的.  相似文献   

RADIOGRAPHIC ANATOMICAL INSIGHTS INTO MECHANISM OF LOWER CALYCEAL STONE EVACUATION FOLLOWING ESWL     

谢立平  史时芳 《Journal of Zhejiang University. Science. B》2000,(3)

ＩＮＴＲＯＤＵＣＴＩＯＮＳｉｎｃｅｉｔｓｃｌｉｎｉｃａｌｉｎｔｒｏｄｕｃｔｉｏｎ ,ｅｘｔｒａｃｏｒｐｏｒｅａｌｓｈｏｃｋｗａｖｅｌｉｔｈｏｔｒｉｐｓｙ(ＥＳＷＬ)ｈａｓｍａｉｎｔａｉｎｅｄａｎｉｍｐｏｒｔａｎｔｒｏｌｅｉｎｔｈｅｍａｎａｇｅｍｅｎｔｏｆｕｒｉｎａｒｙｃａｌｃｕｌｉ.Ｔｈｅｓｕｃｃｅｓｓｏｆｔｈｉｓｔｈｅｒａｐｅｕｔｉｃｐｒｏｃｅ ｄｕｒｅｄｅｐｅｎｄｓｏｎｔｈｅｅｆｆｅｃｔｉｖｅｄｉｓｉｎｔｅｇｒａｔｉｏｎａｎｄｅｖａｃｕａｔｉｏｎｏｆｓｔｏｎｅｄｅｂｒｉｓ.Ｈｏｗｅｖｅｒ,ｔｈｅｓｔ…  相似文献