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1.
摘要：目的：以神经元和神经胶质细胞相关神经生物学变化为切入点，探讨有氧运动对慢性应激大鼠脑海马区神经可塑性的影响。方法：以SD大鼠为研究对象，按照体重均衡分为对照组（CON）、慢性应激模型组（MOD）、氟西汀组（FLU）、低强度运动组（LIR）和中等强度运动组（MIR）。给予大鼠4周慢性应激，并分别伴随氟西汀给药、低强度或者中等强度的运动干预。4周后，通过开场实验、蔗糖饮水实验、新奇抑制摄食实验以及新物体识别实验检测运动对慢性应激大鼠抑郁样行为、焦虑样行为以及认知障碍的影响；同时，利用酶联免疫法和蛋白免疫印迹法，检测运动对慢性应激大鼠血清皮质酮、海马内前炎因子IL1-β，以及蛋白GFAP、S100β、BDNF、p-ERK、p-CREB表达的影响，探讨运动抗抑郁的行为学效应和作用机制。结果：1）低强度和中等强度运动能够显著增加慢性应激大鼠的开场活动、缩短新奇抑制摄食潜伏期，同时提高慢性应激大鼠新物体的识别指数；2）运动有效逆转了慢性应激大鼠血清皮质酮的异常升高，同时降低海马内前炎性因子IL1-β的分泌；3）运动可显著星形胶质细胞的标志性功能蛋白GFAP和S100β的表达水平，增强慢性应激大鼠的BDNF/ERK/CREB信号转导通路中重要蛋白BDNF、p-ERK、p-CREB的表达。结论：运动可以通过提高慢性应激大鼠脑海马区神经可塑性来达到抗抑郁的目的。可能与以下因素有关：1）运动调节血清皮质酮的异常升高，降低海马内前炎性因子的释放，同时，提高与神经胶质细胞相关蛋白GFAP和S100β活性，从而起到神经元保护的作用；2）运动可以通过激活CREB，BDNF以及ERK等重要信号蛋白的表达，提高神经营养作用，促进神经元的生长，增加神经可塑性和神经发生来发挥抗抑郁作用。 相似文献
2.
Li-na Yu Jing Yu Feng-jiang Zhang Mei-juan Yang Ting-ting Ding Jun-kuan Wang Wei He Tao Fang Gang Chen Min Yan 《Journal of Zhejiang University. Science. B》2010,11(9):661-672
Sevoflurane postconditioning reduces myocardial infarct size.The objective of this study was to examine the role of the phosphatidylinositol-3-kinase(PI3K)/Akt pathway in anesthetic postconditioning and to determine whether PI3K/Akt signaling modulates the expression of pro-and antiapoptotic proteins in sevoflurane postconditioning.Isolated and perfused rat hearts were prepared first,and then randomly assigned to the following groups:Sham-operation(Sham),ischemia/reperfusion(Con),sevoflurane postconditioning(SPC),Sham plus 100 nmol/L wortmannin(Sham+Wort),Con+Wort,SPC+Wort,and Con+dimethylsulphoxide(DMSO).Sevoflurane postconditioning was induced by administration of sevoflurane(2.5%,v/v) for 10 min from the onset of reperfusion.Left ventricular developed pressure(LVDP),left ventricular end-diastolic pressure(LVEDP),maximum increase in rate of LVDP(+dP/dt),maximum decrease in rate of LVDP(?dP/dt),heart rate(HR),and coronary flow(CF) were measured at baseline,R30 min(30 min of reperfusion),R60 min,R90 min,and R120 min.Creatine kinase(CK) and lactate dehydrogenase(LDH) were measured after 5 min and 10 min reperfusion.Infarct size was determined by triphenyltetrazolium chloride staining at the end of reperfusion.Total Akt and phosphorylated Akt(phospho-Akt),Bax,Bcl-2,Bad,and phospho-Bad were determined by Western blot analysis.Analysis of variance(ANOVA) and Student-Newman-Keuls' test were used to investigate the significance of differences between groups.The LVDP,±dP/dt,and CF were higher and LVEDP was lower in the SPC group than in the Con group at all points of reperfusion(P0.05).The SPC group had significantly reduced CK and LDH release and decreased infarct size compared with the Con group [(22.9±8)% vs.(42.4±9.4)%,respectively;P0.05].The SPC group also had increased the expression of phospho-Akt,Bcl-2,and phospho-Bad,and decreased the expression of Bax.Wortmannin abolished the cardioprotection of sevoflurane postconditioning.Sevoflurane postconditioning may protect the isolated rat heart.Activation of PI3K and modulation of the expression of pro-and antiapoptotic proteins may play an important role in sevoflurane-induced myocardial protection. 相似文献
3.
访谈了中学教师32人、中学生58人、家长21人对青少年的期望。结合质化和量化两种研究取向,对三个群体所持期望的异同进行比较分析,得出结果发现：不同群体对青少年的期望都包括了人格发展、知识能力、人际关系和身体外貌四个大的类属。除了身体外貌之外,其余三个大类属下面分别包含了五个小的类属。三个群体中,青少年更强调人格发展,对知识能力、人际关系和身体外貌三个方面的强调较少;父母更强调知识能力、人际关系和身体外貌,对人格发展强调得最少;教师则处于中间位置;并对各大类属下的小类属进行了异同比较。 相似文献
4.
科技基础性工作数据资料的汇交与整编 总被引：1，自引：0，他引：1
诸云强 孙 凯杨雅萍 王筱萱乐夏芳 朱华忠宋 佳何明跃 陈佑启彭克银 付 磊 杨彦臣 杨 眉 邹金秋陈 艳 李金斌 刘 燕 雷 蕾 杨 杰李威蓉 张金区 《中国科技资源导刊 (中国信息导报)》2017,(5):12-20
国家科技计划项目数据资料汇交与规范化整编对于促进数据的开放共享,发挥数据资源的最大价值,实现
国家科技投入的最大效益具有重要意义。科技基础性工作专项是国家科技计划的重要组成部分,其数据资料尚未得到
有效的汇交与规范化整编。基于此,本文首先研究科技基础性工作专项项目数据资料汇交与规范化整编的总体流程,
然后论述了数据汇交和规范化整编的具体内容和实施步骤,最后开展了数据资料汇交与规范化整编实践。本文的研究
思路对于国家各级科技计划项目数据资料的汇交与规范化整编具有重要的参考意义。 相似文献
5.
本研究的目的是建立中国人的性别角色量表，并探索不同性别角色类型的个体其心理社会适应水平的特点。研究一根据5000多名被试对中国人人格量表项目的反应挑选出男女被试有显著差异的项目，经过因素分析建立了各由30个项目组成，分别由七个和六个因素构成的中国人男性化和女性化量表，并据此将被试划分为未分化、女性化、男性化和双性化四种性别角色类型。研究二则比较了不同性别角色类型与心理健康的关系，表明在中国被试中双性化的个体心理社会适应水平最低、女性化个体心理社会适应最好。这一结果与Bem关于双性化的假设是截然相反的。本研究从中国文化与性别角色的关系对这一结果进行了解释，提出了中国文化是女性化文化，同时又是反男性化文化的假设，并对进一步研究的方向进行了讨论。 相似文献
6.
Liu Ai-jun Furusato Bungo Ravindranath Lakshmi Chen Yong-mei Srikantan Vasanta Mcleod David G. Petrovics Gyorgy Srivastava Shiv 《Journal of Zhejiang University. Science. B》2007,8(12):853-859
Objective To investigate molecular alterations associating with prostate carcinoma progression and potentially provide information toward
more accurate prognosis/diagnosis.
Methods A set of laser captured microdissected (LCM) specimens from 300 prostate cancer (PCa) patients undergoing radical prostatectomy
(RP) were defined. Ten patients representing “aggressive” PCa, and 10 representing “non-aggressive” PCa were selected based
on prostate-specific antigen (PSA) recurrence, Gleason score, pathological stage and tumor cell differentiation, with matched
patient age and race between the two groups. Normal and neoplastic prostate epithelial cells were collected with LCM from
frozen tissue slides obtained from the RP specimens. The expressions of a panel of genes, including NPY, PTEN, AR, AMACR, DD3, and GSTP1, were measured by quantitative real-time RT-PCR (TaqMan), and correlation was analyzed with clinicopathological features.
Results The expressions of AMACR and DD3 were consistently up-regulated in cancer cells compared to benign prostate epithelial cells in all PCa patients, whereas
GSTP1 expression was down regulated in each patient. NPY, PTEN and AR exhibited a striking difference in their expression patterns between aggressive and non-aggressive PCas (P=0.0203, 0.0284, and 0.0378, respectively, Wilcoxon rank sum test). The lower expression of NPY showed association with “aggressive” PCas based on a larger PCa patient cohort analysis (P=0.0037, univariate generalized linear model (GLM) analysis).
Conclusion Despite widely noted heterogeneous nature of PCa, gene expression alterations of AMACR, DD3, and GSTP1 in LCM-derived PCa epithelial cells suggest for common underlying mechanisms in the initiation of PCa. Lower NPY expression level is significantly associated with more aggressive clinical behavior of PCa; PTEN and AR may have potential in defining PCa with aggressive clinical behavior. Studies along these lines have potential to define
PCa-associated gene expression alterations and likely co-regulation of genes/pathways critical in the biology of PCa onset/progression.
Project supported by the Center for Prostate Disease Research, and the Henry M. Jackson Foundation for the Advancement of
Military Medicine, Rockville, MD, USA 相似文献
7.
For cancer patients, the enumeration of rare circulating tumor cells (CTCs) in peripheral blood is a strong prognostic indicator of the severity of the cancer; for the general population, the capture of CTCs is needed for use as a clinical tool for cancer screening, early detection, and treatment assessment. Here, we present a fast, high-purity (∼90%) and high-efficiency (>90%) method for the segregation and undamaged recovery of CTCs using a spatially gradated microfluidic chip. Further, by lysing the red blood cells we achieved not only a significant reduction in the overall processing time but also mitigated the blood clogging problem commonly encountered in microfluidic-based CTC isolation systems. To clinically validate the chip, we employed it to detect and capture CTCs from 10 liver cancer patients. Positive CTC enumeration was observed in all the blood samples, and the readings ranged from a low of 1–2 CTCs (1 patient) to a high of >20 CTCs (2 patients) with the balance having 3–20 CTCs per 3-ml blood sample. The work here indicates that our system can be developed for use in cancer screening, metastatic assessment, and chemotherapeutic response and for pharmacological and genetic evaluation of single CTCs. 相似文献
8.
Geng QIAN Hong-bin LIU Jin-wen WANG Chen WU Yun-dai CHEN 《Journal of Zhejiang University. Science. B》2013,14(8):736-742
Although cardiac rupture (CR) is a fatal mechanical complication of acute myocardial infarction (AMI), to date no predictive model for CR has been described. CR has common pathological characteristics with major bleeding. We aimed to investigate the relationship between the risk factors of major bleeding and CR. A total of 10 202 consecutive AMI patients were recruited, and mechanical complications occurred in 72 patients. AMI patients without CR were chosen as control group. Clinical characteristics including bleeding-related factors were compared between the groups. The incidences of free wall rupture (FWR), ventricular septal rupture (VSR), and papillary muscle rupture (PMR) were 0.39%, 0.21%, and 0.09%, respectively, and the hospital mortalities were 92.5%, 45.5%, and 10.0%, respectively. Female proportion and average age were significantly higher in the groups of FWR and VSR than in the control group (P<0.01); higher white blood cell count and lower hemoglobin were found in all CR groups (P<0.01). Compared to the control group, patients with CR were more likely to receive an administration of thrombolysis [26.39% vs. 13.19%, P<0.05], and were less likely to be treated with primary percutaneous coronary intervention (PCI) [41.67% vs. 81.60%, P<0.05]. The major bleeding scores (integer scores) of FWR, VSR, and PMR were (17.70±7.24), (21.91±8.33), and (18.60±7.88), respectively, and were significantly higher than that of the control group (11.72±7.71) (P<0.05). A regression analysis identified age, increased heart rate, anemia, higher white blood cell count, and thrombolysis as independent risk factors of CR, most of which were major bleeding-related factors. The patients with CR have a significantly higher risk of hemorrhage compared to the group without CR. Risk of CR after AMI is related to the risk of hemorrhage. 相似文献
9.
李笑一 《内蒙古师范大学学报(哲学社会科学版)》2011,40(3):138-141
在西方世界,新闻记者拥有＂无冕之王＂的地位,而新闻界被称作是＂无冕国度＂。新闻报道往往能够使本国甚至世界上其他相隔遥远的地方发生的事情在瞬间变得全球皆知。而新闻报道还有一重要作用就是对国家对外政策产生影响。新闻记者通过种种尖端的信息传播技术探求事实并公布于众,尤其当国家处于非常时刻,可以在政治传播领域扮演议程设置者的角色,从而对于塑造民意起不可估量的作用。因此,与理论相对的就是政治家的回应和控制。 相似文献
10.
《运动与健康科学(英文)》2022,11(4):495-508
BackgroundAngiogenesis constitutes a major mechanism responsible for exercise-induced beneficial effects. Our previous study identified a cluster of differentially expressed extracellular vesicle microRNAs (miRNAs) after exercise and found that some of them act as exerkines. However, whether these extracellular vesicle miRNAs mediate the exercise-induced angiogenesis remains unknown.MethodsA 9-day treadmill training was used as an exercise model in C57BL/6 mice. Liver-specific adeno-associated virus 8 was used to knock down microRNA-122-5p (miR-122-5p). Human umbilical vein endothelial cells were used in vitro.ResultsAmong these differentially expressed extracellular vesicle miRNAs, miR-122-5p was identified as a potent pro-angiogenic factor that activated vascular endothelial growth factor signaling and promoted angiogenesis both in vivo and in vitro. Exercise increased circulating levels of miR-122-5p, which was produced mainly by the liver and shuttled by extracellular vesicles in mice. Inhibition of circulating miR-122-5p or liver-specific knockdown of miR-122-5p significantly abolished the exercise-induced pro-angiogenic effect in skeletal muscles, and exercise-improved muscle performance in mice. Mechanistically, miR-122-5p promoted angiogenesis through shifting substrate preference to fatty acids in endothelial cells, and miR-122-5p upregulated endothelial cell fatty-acid utilization by targeting 1-acyl-sn-glycerol-3-phosphate acyltransferase (AGPAT1). In addition, miR-122-5p increased capillary density in perilesional skin tissues and accelerated wound healing in mice.ConclusionThese findings demonstrated that exercise promotes angiogenesis through upregulation of liver-derived extracellular vesicle miR-122-5p, which enhances fatty acid utilization by targeting AGPAT1 in endothelial cells, highlighting the therapeutic potential of miR-122-5p in tissue repair. 相似文献