Effects of purposeful soccer heading on circulating small extracellular vesicle concentration and cargo     

Eric R. Muoz  Jaclyn B. Caccese  Brittany E. Wilson  Kyle T. Shuler  Fernando V. Santos  Carolina T. Cabn  John J. Jeka  Dianne Langford  Matthew B. Hudson 《运动与健康科学(英文)》2021,10(2):122

BackgroundConsidering the potential cumulative effects of repetitive head impact (HI) exposure, we need sensitive biomarkers to track short- and long-term effects. Circulating small extracellular vesicles (sEVs) (<200 nm) traffic biological molecules throughout the body and may have diagnostic value as biomarkers for disease. The purpose of this study was to identify the microRNA (miRNA) profile in circulating sEVs derived from human plasma following repetitive HI exposure.MethodsHealthy adult (aged 18–35 years) soccer players were randomly assigned to one of 3 groups: the HI group performed 10 standing headers, the leg impact group performed 10 soccer ball trapping maneuvers over 10 min, and the control group did not participate in any soccer drills. Plasma was collected before testing and 24 h afterward, and sEVs were isolated and characterized via nanoparticle tracking analysis. Next-generation sequencing was utilized to identify candidate miRNAs isolated from sEVs, and candidate microRNAs were analyzed via quantitative polymerase chain reaction. In silico target prediction was performed using TargetScan (Version 7.0; targetscan.org) and miRWalk (http://mirwalk.umm.uni-heidelberg.de/) programs, and target validation was performed using luciferase reporter vectors with a miR-7844-5p mimic in human embryonic kidney (HEK) 293T/17 cells.ResultsPlasma sEV concentration and size were not affected across time and group following repetitive HI exposure. After 24 h, the HI read count from next-generation sequencing showed a 4-fold or greater increase in miR-92b-5p, miR-423-5p, and miR-24-3p and a 3-fold or greater decrease in miR-7844-5p, miR-144-5p, miR-221-5p, and miR-22-3p. Analysis of quantitative polymerase chain reaction revealed that leg impact did not alter the candidate miRNA levels. To our knowledge, miR-7844-5p is a previously unknown miRNA. We identified 8 miR-7844-5p mRNA targets: protein phosphatase 1 regulatory inhibitor subunit 1B (PPP1R1B), LIM and senescent cell antigen-like domains 1 (LIMS1), autophagy-related 12 (ATG12), microtubule-associated protein 1 light chain 3 beta (MAP1LC3B), integrin subunit alpha-1 (ITGA1), mitogen-activated protein kinase 1 (MAPK1), glycogen synthase kinase 3β (GSK3β), and mitogen-activated protein kinase 8 (MAPK8).ConclusionCollectively, these data indicate repetitive HI exposure alters plasma sEV miRNA content, but not sEV size or number. Furthermore, for the first time we demonstrate that previously unknown miR-7844-5p targets mRNAs known to be involved in mitochondrial apoptosis, autophagy regulation, mood disorders, and neurodegenerative disease.  相似文献   

Revisiting the cluster-based paradigm for implicit search result diversification     

Hai-Tao Yu  Adam Jatowt  Roi Blanco  Hideo Joho  Joemon M. Jose  Long Chen  Fajie Yuan 《Information processing & management》2018,54(4):507-528

To cope with ambiguous and/or underspecified queries, search result diversification (SRD) is a key technique that has attracted a lot of attention. This paper focuses on implicit SRD, where the subtopics underlying a query are unknown. Many existing methods appeal to the greedy strategy for generating diversified results. A common practice is using a heuristic criterion for making the locally optimal choice at each round. As a result, it is difficult to know whether the failures are caused by the optimization criterion or the setting of parameters. Different from previous studies, we formulate implicit SRD as a process of selecting and ranking k exemplar documents through integer linear programming (ILP). The key idea is that: for a specific query, we expect to maximize the overall relevance of the k exemplar documents. Meanwhile, we wish to maximize the representativeness of the selected exemplar documents with respect to the non-selected documents. Intuitively, if the selected exemplar documents concisely represent the entire set of documents, the novelty and diversity will naturally arise. Moreover, we propose two approaches ILP4ID (Integer Linear Programming for Implicit SRD) and AP4ID (Affinity Propagation for Implicit SRD) for solving the proposed formulation of implicit SRD. In particular, ILP4ID appeals to the strategy of bound-and-branch and is able to obtain the optimal solution. AP4ID being an approximate method transforms the target problem as a maximum-a-posteriori inference problem, and the message passing algorithm is adopted to find the solution. Furthermore, we investigate the differences and connections between the proposed models and prior models by casting them as different variants of the cluster-based paradigm for implicit SRD. To validate the effectiveness and efficiency of the proposed approaches, we conduct a series of experiments on four benchmark TREC diversity collections. The experimental results demonstrate that: (1) The proposed methods, especially ILP4ID, can achieve substantially improved performance over the state-of-the-art unsupervised methods for implicit SRD. (2) The initial runs, the number of input documents, query types, the ways of computing document similarity, the pre-defined cluster number and the optimization algorithm significantly affect the performance of diversification models. Careful examinations of these factors are highly recommended in the development of implicit SRD methods. Based on the in-depth study of different types of methods for implicit SRD, we provide additional insight into the cluster-based paradigm for implicit SRD. In particular, how the methods relying on greedy strategies impact the performance of implicit SRD, and how a particular diversification model should be fine-tuned.  相似文献   

1982 FIFA World Cup: from Spain’s struggle for democratic legitimacy to Italy’s rediscovery of football nationalism     

Juan Antonio Simón 《Soccer & Society》2019,20(7-8):948-959

ABSTRACT

One of the most memorable moments in the World Cups history is Marco Tardelli’s second goal in Italy’s win over Germany in the 1982 World Cup finals. Better known as Tardelli’s scream, it is thus a perfect object to analyse in order to understand football’s deeper social meanings and its political and cultural undertones, in this case both for the World Cup champions, Italy, and for the host country, Spain. In the Spanish case, organizing the World Cup was a huge political opportunity for the just restored democracy to showcase to the world a modern and pluralistic society leaving behind the authoritarian and outdated dictatorship. As for Italy, their first World Cup winning since World War II was heavily politically used by actors across the political spectrum. Such nationalistic approach constituted a novelty after decades of a low profile national discourse.  相似文献   

Quality flaw prediction in Spanish Wikipedia: A case of study with verifiability flaws     

Edgardo Ferretti  Leticia Cagnina  Viviana Paiz  Sebastián Delle Donne  Rodrigo Zacagnini  Marcelo Errecalde 《Information processing & management》2018,54(6):1169-1181

In this work, we present the first quality flaw prediction study for articles containing the two most frequent verifiability flaws in Spanish Wikipedia: articles which do not cite any references or sources at all (denominated Unreferenced) and articles that need additional citations for verification (so-called Refimprove). Based on the underlying characteristics of each flaw, different state-of-the-art approaches were evaluated. For articles not citing any references, a well-established rule-based approach was evaluated and interesting findings show that some of them suffer from Refimprove flaw instead. Likewise, for articles that need additional citations for verification, the well-known PU learning and one-class classification approaches were evaluated. Besides, new methods were compared and a new feature was also proposed to model this latter flaw. The results showed that new methods such as under-bagged decision trees with sum or majority voting rules, biased-SVM, and centroid-based balanced SVM, perform best in comparison with the ones previously published.  相似文献   

The role of task and ego-oriented climate in explaining students’ bright and dark motivational experiences in Physical Education     

Luis García-González  Javier Sevil-Serrano  Angel Abós  Nathalie Aelterman  Leen Haerens 《Physical Education & Sport Pedagogy》2019,24(4):344-358

Background: A distinction is made in Achievement Goal Theory (AGT) between task-oriented (i.e. effort, intra-individual progress, and self-comparison) and ego-oriented (i.e. inter-individual progress and normative comparison) climates. Combining insights from AGT and Self-Determination Theory (SDT), studies in the PE context have shown that a task-oriented climate positively relates to need satisfaction, although the findings regarding the motivating role of an ego-oriented climate are inconsistent. Moreover, little is known about the role of task- or ego-oriented climates in explaining experiences of basic psychological need frustration.

Purpose: Grounded in AGT and SDT, the aim of the present study was to examine if experiences of basic psychological need satisfaction and need frustration can explain why task- and ego-oriented climates elicit positive and negative motivational outcomes in PE, respectively.

Research design: Cross-sectional study.

Method: A sample of 524 secondary school students (51.1% boys, M_age?=?14.51; SD?=?1.81) from five different secondary schools participated in this study. Students reported on their perceptions of task- and ego-oriented climates, motivational regulations, basic psychological need satisfaction, and need frustration, as well as positive and negative outcomes in PE. Structural equation modeling was used to investigate our objective.

Results: We found that a task-oriented climate had a strong and positive relationship with basic psychological need satisfaction, eliciting a bright pathway to autonomous motivation and affective attitude. An ego-oriented climate was positively related to basic psychological need frustration, eliciting a dark pathway to amotivation and boredom. A negative cross-path from task-oriented climate to basic psychological need frustration was also found, while no significant cross-paths were found from ego-oriented climate to basic psychological need satisfaction.

Conclusions: This study provides a better understanding of the mechanisms that explain why task- and ego-oriented climates shape students’ motivational experiences in PE lessons. It is suggested that a task-oriented climate elicits a bright pathway towards more optimal functioning, because it fosters experiences of need satisfaction and buffers against experiences of need frustration. In contrast, an ego-oriented climate is primarily positively related to feelings of need frustration and negative motivational outcomes. Practical implications for PE teacher training are discussed.  相似文献   

Effect of different loading conditions on running mechanics at different velocities     

Germán Carretero-Navarro  Doménico Cherubini  Wolfgang Taube 《European Journal of Sport Science》2019,19(5):595-602

Weighted vests are widely used to improve running economy and performance. However, it is not well-studied how running mechanics are adapted to counteract the higher peak vertical ground reaction forces (Fpeak) while running with such a device. Therefore, the present study aimed to investigate the effects of different loading conditions on running mechanics at different velocities. Thirteen subjects participated in two separate sessions one week apart. In the first session, maximal aerobic speed (MAS) was determined through a maximal incremental running test while in the second session, they were instructed to run during one minute under different loading (0%, +10% and +20% of body mass [BM]) and velocity (60%, 80% and 100% of MAS) conditions in a random order. Spatiotemporal data were recorded and then running mechanics modelled using the spring-mass model. The main results indicated that vertical and leg stiffness (Kvert and Kleg, respectively) were increased (P?P?>?.05) when load was changed. At the same time, alterations of the running kinematics were observed such as longer contact times, reduced flight times, stride frequencies and step lengths, as well as an increase of the centre of mass dynamics. Based on these results it is assumed that runners maintain a certain stiffness level for each velocity despite different loading conditions. As a consequence, Fpeak increases and this probably causes spatiotemporal adjustments in the movement kinematics.  相似文献   

Effects of blood flow restriction without additional exercise on strength reductions and muscular atrophy following immobilization:A systematic review     

Mikhail Santos Cerqueira  Jose Diego Sales Do Nascimento  Daniel Germano Maciel  Jean Artur Mendonca Barboza  Wouber Herickson De Brito Vieira 《运动与健康科学(英文)》2020,9(2):152-159

Purpose:To investigate whether blood flow restriction(BFR) without concomitant exercise mitigated strength reduction and atrophy of thigh muscles in subjects under immobilization for lower limbs.Methods:The following databases were searched:PubMed,CINAHL,PEDro,Web of Science,Central,and Scopus.Results:The search identified 3 eligible studies,and the total sample in the identified studies consisted of 38 participants.Isokinetic and isometric torque of the knee flexors and extensors was examined in 2 studies.Cross-sectional area of thigh muscles was evaluated in 1 study,and thigh girth was measured in 2 studies.The BFR protocol was 5 sets of 5 min of occlusion and 3 min of free flow,twice daily for approximately 2 weeks.As a whole,the included studies indicate that BFR without exercise is able to minimize strength reduction and muscular atrophy after immobilization.It is crucial to emphasize,however,that the included studies showed a high risk of bias,especially regarding allocation concealment,blinding of outcome assessment,intention-to-treat analyses,and group similarity at baseline.Conclusion:Although potentially useful,the high risk of bias presented by original stodies limits the indication of BFR without concomitant exercise as an effective countermeasure against strength reduction and atrophy mediated by immobilization.  相似文献   

The load-velocity profile differs more between men and women than between individuals with different strength levels     

Alejandro Torrejón  Carlos Balsalobre-Fernández  G. Gregory Haff 《Sports biomechanics / International Society of Biomechanics in Sports》2019,18(3):245-255

This study aimed to determine the suitability of the load-velocity relationship to prescribe the relative load (%1RM) in women, as well as to compare the load-velocity profile between sexes and participants with different strength levels. The load-velocity relationship of 14 men (1RM: 1.17 ± 0.19) and 14 women (1RM: 0.66 ± 0.13) were evaluated in the bench press exercise. The main findings revealed that: (I) the load-velocity relationship was always strong and linear (R² range: 0.987–0.993), (II) a steeper load-velocity profile was observed in men compared to women (Effect size [ES]: 1.09), with men showing higher velocities for light loads (ES: ? 0.81 and ? 0.40 for the y-intercept and 30%1RM, respectively), but women reporting higher velocities for the heavy loads (ES: 1.14 and 1.50 at 90%1RM and 100%1RM, respectively); and (III) while the slope of the load-velocity profile was moderately steeper for weak men compared to their strong counterpart (ES: 1.02), small differences were observed between strong and weak women (ES: ? 0.39). While these results support the use of the individual load-velocity relationship to prescribe the %1RM in the bench press exercise for women, they also highlight the large disparities in their load-velocity profile compared to men.  相似文献   

The Ultimate Beneficiaries of Continuing Professional Development Programs: Middle School Students’ Nature of Science Views     

&#;zer  Ferah  Do&#;an  Nihal  Yalaki  Yal&#;&#;n  Irez  Serhat  &#;akmakci  G&#;ltekin 《Research in Science Education》2019,51(2):757-782

The effective continuing professional development (CPD) programs primarily aim to have an impact on teachers’ knowledge bases, beliefs, and views and their classroom practices, which rationally lead most of the researchers to investigate those changes on teachers primarily. Although neglected, the interrelationship between CPD programs and students is considered complex, and CPDs ultimately aim to have an impact on students’ views, too. Therefore, the purpose of this study is to survey the changes of middle school students’ views of the nature of science (NOS) by providing a large-scale CPD to their teachers and, in other words, identify the impact of CPD on ultimate beneficiaries, namely students. In this study, 10 science teachers’ data and, in the first phase, 481 and, in the second phase, 422 students’ data and the changes in their NOS views were analyzed. Results showed that the students’ and teachers’ NOS views changed positively. For the impact of teachers on the students’ views, the teachers’ prior NOS knowledge, years of experience, and the number of implemented activities were found to be the influential factors for the transmission of NOS views.

  相似文献   

A qualitative investigation of design knowledge reuse in project-based mechanical design courses     

Dónal P. Holland  Conor J. Walsh 《European Journal of Engineering Education》2019,44(1-2):137-152

ABSTRACT

The interpretation and reuse of previous design solutions, or precedents, is central to design. This paper describes qualitative research conducted over three years at two institutions, aimed at investigating the role of design knowledge re-use in project-based mechanical design courses. Research data were collected through participant observation, student interviews, anonymous questionnaires, and website analytics. The paper identifies challenges that must be addressed in order to support novice engineers in rehearsing the types of knowledge required to successfully reason about and engage with design precedents. Two categories of design precedents are identified: concept precedents and detail precedents. Providing students with access to the latter is identified as a particular challenge, as is providing students with access to engineering communities of practice. Approaches to addressing these challenges are discussed.  相似文献