Production and Characterization of α-Amylase from an Extremely Halophilic Archaeon,Haloferax sp. HA10     

Bhakti Bajpai  Monika Chaudhary  Jyoti Saxena 《Food Technology and Biotechnology》2015,53(1):11-17

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Demonstration of a new biosensing concept for immunodiagnostic applications based on change in surface conductance of antibodies after biomolecular interactions     

Vashist SK  Kaur I  Bajpai RP  Bharadwaj LM  Tewari R  Raiteri R 《Journal of Zhejiang University. Science. B》2006,7(9):683-685

We report an important observation that the surface conductivity of antibody layer immobilized on polylysine-coated glass substrate decreases upon the formation of complex with their specific antigens. This change in conductivity has been observed for both monoclonal and polyclonal antibodies. The conductance of monoclonal mouse IgG immobilized on polylysine-coated glass substrate changed from 1.02×10⁻⁸ Ω⁻¹ to 1.41×10⁻¹¹ Ω⁻¹ at 10 V when complex is formed due to the specific biomolecular interactions with rabbit anti-mouse IgG F(ab′)₂. Similar behavior was observed when the same set up was tested in two clinical assays: (1) anti-Leishmania antigen polyclonal antibodies taken from Kala Azar positive patient serum interacting with Leishmania promastigote antigen, and (2) anti-p21 polyclonal antibodies interacting with p21 antigen. The proposed concept can represent a new immunodiagnostic technique and may have wide ranging applications in biosensors and nanobiotechnology too.  相似文献   

Monitoring and Root Cause Analysis of Clinical Biochemistry Turn Around Time at an Academic Hospital     

Kiran P. Chauhan  Amit P. Trivedi  Dharmik Patel  Bhakti Gami  N. Haridas 《Indian journal of clinical biochemistry : IJCB》2014,29(4):505-509

Quality can be defined as the ability of a product or service to satisfy the needs and expectations of the customer. Laboratories are more focusing on technical and analytical quality for reliability and accuracy of test results. Patients and clinicians however are interested in rapid, reliable and efficient service from laboratory. Turn around time (TAT), the timeliness with which laboratory personnel deliver test results, is one of the most noticeable signs of laboratory service and is often used as a key performance indicator of laboratory performance. This study is aims to provide clue for laboratory TAT monitoring and root cause analysis. In a 2 year period a total of 75,499 specimens of outdoor patient department were monitor, of this a total of 4,142 specimens exceeded TAT. With consistent efforts to monitor, root cause analysis and corrective measures, we are able to decreased the specimens exceeding TAT from 7–8 to 3.7 %. Though it is difficult task to monitor TAT with the help of laboratory information system, real time documentation and authentic data retrievable, along with identification of causes for delays and its remedial measures, improve laboratory TAT and thus patient satisfaction.  相似文献