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1.

Introduction

Total 25-hydroxyvitamin D [25(OH)D] is the most reliable indicator of vitamin D status. In this study, we compared two automated immunoassay methods, the Abbott Architect 25-OH Vitamin D assay and the Roche Cobas Vitamin D total assay, with the liquid chromatography-tandem mass spectrometry (LC-MS/MS).

Materials and methods

One hundred venous blood samples were randomly selected from routine vitamin D tests. Two of the serum aliquots were analyzed at the Abbott Architect i2000 and the Roche Cobas 6000’s module e601 in our laboratory within the same day. The other serum aliquots were analyzed at the LC-MS/MS in different laboratory. Passing-Bablok regression analysis and Bland-Altman plot were used to compare methods. Inter-rater agreement was analyzed using kappa (κ) analysis.

Results

The Roche assay showed acceptable agreement with the LC-MS/MS based on Passing-Bablok analysis (intercept: -5.23 nmol/L, 95% CI: -8.73 to 0.19; slope: 0.97, 95% CI: 0.77 to 1.15). The Abbott assay showed proportional (slope: 0.77, 95% CI: 0.67 to 0.85) and constant differences (intercept: 17.08 nmol/L; 95% CI: 12.98 to 21.39). A mean bias of 15.1% was observed for the Abbott and a mean bias of -14.1% was observed for the Roche based on the Bland-Altman plots. We found strong to nearly perfect agreement in vitamin D status between the immunoassays and LC-MS/MS. (κ: 0.83 for Abbott, κ: 0.93 for Roche) using kappa analysis.

Conclusion

Both immunoassays demonstrated acceptable performance, but the Roche Cobas assay demonstrated better performance than the Abbott Architect in the studied samples.Key words: 25-Hydroxyvitamin D, chromatography, immunoassay, methods, tandem mass spectrometry  相似文献   
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This paper is based on a quasi-experimental study which examines the effects of a school-based intervention on Omani girls’ attitudes towards the notion of gender equality. A questionnaire was administered before and after the intervention to 241 girls (116 in the experimental group; 125 in the control group). A semi-structured interview was conducted before and after the intervention with 16 participants (eight from the experimental group; eight from the control group). Analysis of quantitative data in the post-intervention round revealed statistically significant differences between the two groups in attitudes towards gender equality in favour of the experimental group. Similarly, the analysis of qualitative data from the post-intervention round showed that the experimental group differed significantly from the control group in its attitudes towards women’s roles and gender equality. The paper concludes by emphasising the critical role that education can play in challenging and transforming prejudicial and discriminatory attitudes.  相似文献   
4.
Adult overhead athletes without a history of shoulder injury show scapular adaptations. There is a lack of detailed assessment of scapular kinematics in junior overhead athletes. This study aims to investigate three-dimensional scapular kinematics in junior overhead athletes. We recruited a total of 20 junior tennis players and 20 healthy children without participation in any overhead sports in this study. Bilateral scapular kinematic data were recorded using an electromagnetic tracking device for scapular plane glenohumeral elevation. The data were further analysed at 30°, 45°, 60°, 90° and 120° during glenohumeral elevation and lowering. Statistical comparisons of the data between groups (junior overhead athletes and non-overhead athletes) and sides (serve dominant and non-dominant shoulders of the overhead athletes) were analysed with the ANOVA. Comparisons showed that, in general, the scapula was more upwardly rotated and anteriorly tilted in overhead athletes when compared to non-overhead athletes, however there was no side-to-side differences when serve dominant and non-dominant shoulders compared in junior overhead athletes. The serve dominant arm of junior overhead athletes had alternations in scapular kinematics when compared with the non-overhead athletes. These findings provide clinical evaluation implications and the need for clinicians to assess for potential adaptations in junior overhead athletes.  相似文献   
5.
ABSTRACT

The potential relationships between innovation and sustainability processes, in engineering education, is neither obvious nor simple, especially when innovation, generally speaking, is promoted regardless of sustainability. We used UniLaSalle (In January 2016, the ‘Institut Polytechnique LaSalle Beauvais’, a French engineering school, merged with another higher education engineering school (ESITPA, Rouen). Both campuses (Beauvais and Rouen) have a common name: UniLaSalle (www.unilasalle.fr). As the interview began during 2015, this article only describes the situation of the Beauvais campus.) as a ‘living Lab’ to identify and analyse the reasons for the integration of innovation and Sustainable Development (SD) in training programmes. The aim is to explore, in a perception and reflective approach, how SD can be understood as a driver for specific innovation attitudes in higher education engineering school. This paper adopts an empirical approach based on a qualitative study, using NVivo 9 software to analyse non-numerical data. Our research suggests that SD can be a structural driver for innovation if it is integrated in a transdisciplinarity approach and not just as a discipline per se.  相似文献   
6.

Introduction:

Today, the pneumatic tube transport system (PTS) is used frequently because of its advantages related to timing and speed. However, the impact of various types of PTS on blood components is unknown. The aim of this study was to examine the influence of PTS on the quality of routine blood cell counts, erythrocyte sedimentation, and certain blood coagulation tests.

Materials and methods:

Paired blood samples were obtained from each of 45 human volunteers and evaluated by blood cell count, erythrocyte sedimentation, and several coagulation tests, including prothrombin time (PT) and activated partial thromboplastin time (aPTT). Blood samples were divided into 2 groups: Samples from group 1 were transported to the laboratory via the PTS, and samples from group 2 were transported to the laboratory manually. Both groups were evaluated immediately by the tests listed above.

Results:

The blood sample test results from groups 1 and 2 were evaluated and compared. No statistically significant differences were observed (P = 0.069–0.977).

Conclusion:

The PTS yielded no observable effects on blood cell counts, erythrocyte sedimentation, or PT and aPTT test results. We concluded that the PTS can be used to transport blood samples and yield reliable results for blood cell counts, erythrocyte sedimentation, and several coagulation tests.  相似文献   
7.
This paper reports on an attempt to investigate Turkish primary school students’ interest in science by using their self-generated questions. We investigated students’ interest in science by analyzing 1704 self-generated science-related questions. Among them, 826 questions were submitted to a popular science magazine called Science and Children. Such a self-selected sample may represent a group of students who have a higher level of motivation to seek sources of information outside their formal education and have more access to resources than the students of low social classes. To overcome this problem, 739 students were asked to write a question that they wanted to learn from a scientist and as a result 878 questions were gathered. Those students were selected from 13 different schools at 9 cities in Turkey. These schools were selected to represent a mixture of socioeconomic areas and also to cover different students’ profile. Students’ questions were classified into two main categories: the field of interest and the cognitive level of the question. The results point to the popularity of biology, astrophysics, nature of scientific inquiry, technology and physics over other science areas, as well as indicating a difference in interest according to gender, grade level and the setting in which the questions were asked. However, our study suggests that only considering questions submitted to informal learning environments, such as popular science magazines or Ask-A-Scientist Internet sites has limitations and deficiencies. Other methodologies of data collection also need to be considered in designing teaching and school science curriculum to meet students’ needs and interest. The findings from our study tend to challenge existing thinking from other studies. Our results show that self-generated questions asked in an informal and a formal setting have different patterns. Some aspects of students’ self-generated questions and their implications for policy, science curriculum reform and teaching are discussed in this paper.  相似文献   
8.
Every aspect of teaching, including the instructional method, the course content, and the types of assessments, is influenced by teachers’ attitudes and beliefs. Teacher education programs play an important role in the development of beliefs regarding teaching and learning. The purpose of the study was to document pre-service teachers’ views on science, scientists, and science teaching as well as the relations between these views and the offered courses over several years spent in an elementary science teacher training program. The sample consisted of 145 pre-service elementary science teachers who were being trained to teach general science to students in the 6th through 8th grades. The research design was a cross-sectional study. Three different instruments were used to collect the data, namely, the “Draw a Scientist Test”, “Draw a Science Teacher Test”, and “Students’ Views about Science” tests. The elementary science teacher training program influenced pre-service science teachers’ views about science, scientists and science teaching to different degrees. The most pronounced impact of the program was on views about science teaching. Participants’ impressions of science teaching changed from teacher-centered views to student-centered ones. In contrast, participants’ views about scientists and science did not change much. This result could be interpreted as indicating that science teacher training programs do not change views about science and scientists but do change beliefs regarding teaching science.  相似文献   
9.
The aim of this study is to determine the relationship between levels of reading comprehension strategy use, reading attitudes, and the amount of reading per year among elementary school students. The study was conducted with 1316 students (649 girls and 667 boys) attending the fourth and fifth grades of 15 elementary schools in Denizli, Turkey. Stratified Sampling Method was employed to obtain data using Reading Attitude Scale (RAS) and Reading Comprehension Strategies Scale (RCSS). The analyses indicate that reading attitude is a significant predictor of the level of reading comprehension strategies used by students (R2 = 0.44). The findings show that there is a negative and low-level relationship (r = ?0.054) between daily time spent reading and the level of strategy used, and a positive and low-level relationship (r = 0.01) between the level of strategy used and the number of books that a child reads per year.  相似文献   
10.

The purpose of the current study is to investigate the effectiveness of unstructured, semi-structured, structured hands-on modeling activities and traditional teaching methods in developing academic achievement, problem-solving skills, and scientific creativity in prospective science teachers in the subject of the human circulatory and respiratory systems. A pre-test–post-test quasi-experimental design was used to investigate the treatment effect. There were three experimental groups and a control group in a total of 88 prospective science teachers who were enrolled in the Department of Science Education. The Academic Achievement Test (AAT), Problem-Solving Inventory (PSI), and Scientific Creativity Scale (SCS) were applied as data collection tools. The researchers employed two-way ANOVA and ANCOVA to analyze the data. Results revealed that all modeling activities were effective in enhancing participants’ AAT scores when compared with those of the control group. In addition, unstructured modeling and semi-structured modeling activities were more effective than structured modeling activities in improving AAT scores. For the AAT retention test, unstructured and semi-structured modeling groups showed better performance than the structured modeling group and control group. Moreover, there was a statistically significant difference in PSI scores of the participants in favor of unstructured and semi-structured modeling activities. Lastly, there was no statistically significant difference in SCS scores with the experimental groups and control group.

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