Effects of purposeful soccer heading on circulating small extracellular vesicle concentration and cargo     

Eric R. Muoz  Jaclyn B. Caccese  Brittany E. Wilson  Kyle T. Shuler  Fernando V. Santos  Carolina T. Cabn  John J. Jeka  Dianne Langford  Matthew B. Hudson 《运动与健康科学(英文)》2021,10(2):122

BackgroundConsidering the potential cumulative effects of repetitive head impact (HI) exposure, we need sensitive biomarkers to track short- and long-term effects. Circulating small extracellular vesicles (sEVs) (<200 nm) traffic biological molecules throughout the body and may have diagnostic value as biomarkers for disease. The purpose of this study was to identify the microRNA (miRNA) profile in circulating sEVs derived from human plasma following repetitive HI exposure.MethodsHealthy adult (aged 18–35 years) soccer players were randomly assigned to one of 3 groups: the HI group performed 10 standing headers, the leg impact group performed 10 soccer ball trapping maneuvers over 10 min, and the control group did not participate in any soccer drills. Plasma was collected before testing and 24 h afterward, and sEVs were isolated and characterized via nanoparticle tracking analysis. Next-generation sequencing was utilized to identify candidate miRNAs isolated from sEVs, and candidate microRNAs were analyzed via quantitative polymerase chain reaction. In silico target prediction was performed using TargetScan (Version 7.0; targetscan.org) and miRWalk (http://mirwalk.umm.uni-heidelberg.de/) programs, and target validation was performed using luciferase reporter vectors with a miR-7844-5p mimic in human embryonic kidney (HEK) 293T/17 cells.ResultsPlasma sEV concentration and size were not affected across time and group following repetitive HI exposure. After 24 h, the HI read count from next-generation sequencing showed a 4-fold or greater increase in miR-92b-5p, miR-423-5p, and miR-24-3p and a 3-fold or greater decrease in miR-7844-5p, miR-144-5p, miR-221-5p, and miR-22-3p. Analysis of quantitative polymerase chain reaction revealed that leg impact did not alter the candidate miRNA levels. To our knowledge, miR-7844-5p is a previously unknown miRNA. We identified 8 miR-7844-5p mRNA targets: protein phosphatase 1 regulatory inhibitor subunit 1B (PPP1R1B), LIM and senescent cell antigen-like domains 1 (LIMS1), autophagy-related 12 (ATG12), microtubule-associated protein 1 light chain 3 beta (MAP1LC3B), integrin subunit alpha-1 (ITGA1), mitogen-activated protein kinase 1 (MAPK1), glycogen synthase kinase 3β (GSK3β), and mitogen-activated protein kinase 8 (MAPK8).ConclusionCollectively, these data indicate repetitive HI exposure alters plasma sEV miRNA content, but not sEV size or number. Furthermore, for the first time we demonstrate that previously unknown miR-7844-5p targets mRNAs known to be involved in mitochondrial apoptosis, autophagy regulation, mood disorders, and neurodegenerative disease.  相似文献   

TAM and stages of adoption of blended learning in higher education by application of data mining techniques     

Antonio Víctor Martín-García  Fernando Martínez-Abad  David Reyes-González 《British journal of educational technology : journal of the Council for Educational Technology》2019,50(5):2484-2500

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Handwriting production in Spanish children with dyslexia: spelling or motor difficulties?     

Martínez-García  Cristina  Afonso  Olivia  Cuetos  Fernando  Suárez-Coalla  Paz 《Reading and writing》2021,34(3):565-593

Reading and Writing - Spelling and handwriting are different processes; however, they are learned simultaneously, and numerous studies have shown that they interact. Besides the commonly reported...  相似文献   

Battles for technological dominance: an integrative framework   总被引：3，自引：0，他引：3  

Fernando F. Suarez 《Research Policy》2004,33(2):271-286

This paper proposes an integrative framework for understanding the process by which a technology achieves dominance when “battling” against other technological designs. We focus on describing the different stages of a dominance battle and propose five battle milestones that in turn define five key phases in the process. We review the literature from several disciplines to identify the key firm- and environment-level factors that affect the outcome of a technology battle and posit that the relative importance of each factor will vary depending on the phase considered. Our framework complements and extends existing literature and has implications both for theory and for management practice.  相似文献   

An instructional model for web-based e-learning education with a blended learning process approach     

Fernando Alonso  Genoveva López  Daniel Manrique  José M. Viñes 《British journal of educational technology : journal of the Council for Educational Technology》2005,36(2):217-235

Web‐based e‐learning education research and development now focuses on the inclusion of new technological features and the exploration of software standards. However, far less effort is going into finding solutions to psychopedagogical problems in this new educational category. This paper proposes a psychopedagogical instructional model based on content structure, the latest research into information processing psychology and social contructivism, and defines a blended approach to the learning process. Technologically speaking, the instructional model is supported by learning objects, a concept inherited from the object‐oriented paradigm.  相似文献   

Covariation between variables in a modelling process: The ACODESA (collaborative learning,scientific debate and self-reflection) method     

Fernando Hitt  Alejandro S. González-Martín 《Educational Studies in Mathematics》2015,88(2):201-219

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Different Argentine Rural Extensionists' Mindsets and Their Practical Implications     

Fernando Landini 《The Journal of Agricultural Education and Extension》2015,21(3):219-234

Purpose: This paper reflects upon the practice of Argentine rural extensionists working in the extension public system through the process of identifying different rural extensionists' types of mindsets and comparing them with transfer of technology extension approach, dialogical processes of horizontal knowledge exchange, participatory perspectives and innovation system approach.

Design/Methodology/approach: A quali-quantitative investigation was conducted. Surveys containing closed and open questions were sent via email to rural extensionists. This allowed the researcher to identify their beliefs about different issues connected to extension practice. The sample was incidental (n = 219; 143 men, 76 women). Qualitative data was categorized and quantified. Finally, a two-steps cluster analysis was implemented.

Findings: Two types of rural extensionists' mindsets were identified, one of which relates to the transfer of technology approach and other to the dialogical/horizontal model, yet neither of them fitting the ideal of the most important extension institution of the country, which supports an innovation system approach.

Practical implications: Extensionists' practices and institutional ideals do not coincide. Reflexive training processes are required to make beliefs about rural extension explicit in order to build a contextually suitable extension proposal. Originality/Value: Through a quali-quantitative approach to the issue of rural extensionists' mindsets, this paper contributes to a better understanding of Argentine rural extensionists' practices.  相似文献   

Do men and women perform academic work differently?     

González Ramos  Ana M.  Fernández Palacín  Fernando  Muñoz Márquez  Manuel 《Tertiary Education and Management》2015,21(4):263-276

Tertiary Education and Management - Why is the gender gap so large in researchers’ career progression? Do men and women have different priorities in their academic careers? This study...  相似文献   

The Impact of an Erasmus Placement in Students’ Perception of their Intercultural Communicative Competence     

Ramiro Durán Martínez  Gloria Gutiérrez  Fernando Beltrán Llavador  Fernando Martínez Abad 《Journal of Intercultural Communication Research》2016,45(4):338-354

Pre- and post-questionnaires answered by UK Nottingham Trent University (NTU) and Spain’s University of Salamanca (USAL) students on their placement abroad support a comparative analysis of its impact on their intercultural communicative competence, comprising the awareness, attitude, skills and knowledge dimensions. NTU students started their placements with better results in them all. Yet, while both groups completed their stay with a similarly increased awareness, the knowledge and skills of the USAL group matched the results of NTU students and, whereas the scores of the attitude dimension towards the host country of NTU students did not increase significantly, those of USAL students decreased.  相似文献   

Resting Bradycardia,Enhanced Postexercise Heart Rate Recovery and Cardiorespiratory Fitness in Recreational Ballroom Dancers     

Carlos Janssen Gomes da Cruz  Guilherme Eckhardt Molina  Luiz Guilherme Grossi Porto  Luiz Fernando Junqueira Jr 《Research quarterly for exercise and sport》2017,88(3):371-376

Purpose: In a cross-sectional study design, we evaluated the resting heart rate (HR_baseline) and exercise and postexercise stress test-related chronotropic responses in male practitioners of recreational ballroom dancing (BD; n = 25, M_age = 26.6 ± 6.1 years) compared to a control group of insufficiently active nondancers (CG; n = 25, M_age = 25.9 ± 4.5 years). Method: All participants underwent a submaximal exercise test. At 85% of the maximal predicted HR, the recovery protocol was started, and heart rate recovery (HRR) was recorded during 1-min intervals for 5 min. Results: Compared with CG, BD showed lower HR_baseline (70 beats per minute [bpm] vs. 62 bpm, respectively, U = 143, p < .05, ES = .46), lower preexercise HR (94 bpm vs. 86 bpm, U = 157, p < .05, ES = .42), longer exercise test duration (346 s vs. 420 s, U = 95.5, p < .05, ES = .59), and higher HRR for 5 min postexercise (U = 1.29–1.89, p < .05, ES = .33–.50) as follows: 1st min (32 bpm vs. 40 bpm), 2nd min (45 bpm vs. 53 bpm), 3rd min (51 bpm vs. 58 bpm), 4th min (55 bpm vs. 59 bpm), and 5th min (59 bpm vs. 63 bpm). The coefficient of HRR from the 1st min to the 5th min postexercise was similar in both groups (U = 229–311, p > .05, ES = < .10–.22). Conclusion: Heightened cardiovascular functional status characterized by favorable enhanced chronotropic dynamics appears to occur in practitioners of recreational ballroom dancing, which suggests that this modality of exercise may result in health benefits.  相似文献